LOCUS       sequence-304293-       10327 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of cell-surface protein extracellular domain 
            fused to COMP5AP-AviTag-9xHis. Protein is secreted from cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10327)
  AUTHORS   Wojtowicz WM, Vielmetter J, Fernandes RA, Siepe DH, Eastman CL, 
            Chisholm GB, Cox S, Klock H, Anderson PW, Rue SM, Miller JJ, Glaser 
            SM, Bragstad ML, Vance J, Lam AW, Lesley SA, Zinn K, Garcia KC
  TITLE     A Human IgSF Cell-Surface Interactome Reveals a Complex Network of 
            Protein-Protein Interactions.
  JOURNAL   Cell. 2020 Aug 20;182(4):1027-1043.e17. doi: 
            10.1016/j.cell.2020.07.025.
  PUBMED    32822567
REFERENCE   2  (bases 1 to 10327)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2020.07"; journalName: "Cell"; date: "2020-08-20- 
            20"; volume: "182"; issue: "4"; pages: "1027-1043.e17"
COMMENT     Sequence Label: pD649-HAsp-CHL1-COMP5AP-AviTag-9xHis
FEATURES             Location/Qualifiers
     source          1..10327
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     124..143
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     149..168
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     rep_origin      254..842
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     743..762
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     996..1013
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     1111..1128
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     1112..1128
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    1584..1600
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     enhancer        1767..2146
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2147..2350
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2301..2321
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     2347..2371
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        2581..2599
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     2581..2598
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     2612..2631
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     CDS             5932..5967
                     /codon_start=1
                     /product="epitope tag derived from human Protein C (Stearns
                     et al., 1988)"
                     /label=Protein C tag
                     /translation="EDQVDPRLIDGK"
     CDS             5977..6000
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     CDS             7660..7704
                     /codon_start=1
                     /product="peptide tag that allows for enzymatic 
                     biotinylation"
                     /label=AviTag(TM)
                     /translation="GLNDIFEAQKIEWHE"
     CDS             7705..7731
                     /codon_start=1
                     /product="9xHis affinity tag"
                     /label=9xHis
                     /translation="HHHHHHHHH"
     primer_bind     complement(7871..7888)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7877..7988
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(8106..8126)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        8111..8440
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      8291..8426
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     8353..8372
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        8488..8535
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             8554..9153
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(8554..8573)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     9050..9070
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     polyA_signal    9283..9404
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9320..9339)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9374..9393
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     CDS             complement(join(9457..10248,10249..10317))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10080..10099
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(join(10318..10327,1..82))
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa
       61 tgtatttaga aaaataaaca aataggggtc agtgttacaa ccaattaacc aattctgaac
      121 attatcgcga gcccatttat acctgaatat ggctcataac accccttgct catgaccaaa
      181 atcccttaac gtgagttacg cgcgcgtcgt tccactgagc gtcagacccc gtagaaaaga
      241 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa
      301 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga
      361 aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt
      421 tagcccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt
      481 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat
      541 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct
      601 tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca
      661 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
      721 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
      781 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
      841 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
      901 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
      961 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg
     1021 aaggcgagag tagggaactg ccaggcatca aactaagcag aaggcccctg acggatggcc
     1081 tttttgcgtt tctacaaact ctttctgtgt tgtaaaacga cggccagtct taagctcggg
     1141 ccccctgggc ggttctgata acgagtaatc gttaatccgc aaataacgta aaaacccgct
     1201 tcggcgggtt tttttatggg gggagtttag ggaaagagca tttgtcagaa tatttaaggg
     1261 cgcctgtcac tttgcttgat atatgagaat tatttaacct tataaatgag aaaaaagcaa
     1321 cgcactttaa ataagatacg ttgctttttc gattgatgaa cacctataat taaactattc
     1381 atctattatt tatgattttt tgtatataca atatttctag tttgttaaag agaattaaga
     1441 aaataaatct cgaaaataat aaagggaaaa tcagtttttg atatcaaaat tatacatgtc
     1501 aacgataata caaaatataa tacaaactat aagatgttat cagtatttat tatcatttag
     1561 aataaatttt gtgtcgccct taattgtgag cggataacaa ttacgagctt catgcacagt
     1621 ggcgttgact caatattggc cattagccat attattcatt ggttatatag cataaatcaa
     1681 tattggctat tggccattgc atacgttgta tctatatcat aatatgtaca tttatattgg
     1741 ctcatgtcca atatgaccgc catgttggca ttgattattg actagttatt aatagtaatc
     1801 aattacgggg tcattagttc atagcccata tatggagttc cgcgttacat aacttacggt
     1861 aaatggcccg cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta
     1921 tgttcccata gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg
     1981 gtaaactgcc cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga
     2041 cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag tacatgacct tatgggactt
     2101 tcctacttgg cagtacatct acgtattagt catcgctatt accatggtga tgcggttttg
     2161 gcagtacatc aatgggcgtg gatagcggtt tgactcacgg ggatttccaa gtctccaccc
     2221 cattgacgtc aatgggagtt tgttttggca ccaaaatcaa cgggactttc caaaatgtcg
     2281 taacaactcc gccccattga cgcaaatggg cggtaggcgt gtacggtggg aggtctatat
     2341 aagcagagct cgtttagtga accgtcagat cgcctggaga cgccatccac gctgttttga
     2401 cctccataga agacaccggg accgatccag cctccgcggc cgggaacggt gcattggaac
     2461 gcggattccc cgtgccaaga gtgacgtaag taccgcctat agagtctata ggcccacccc
     2521 cttggcttcg ttagaacgcg gctacaatta atacataacc ttatgtatca tacacatacg
     2581 atttaggtga cactatagaa taacatccac tttgcctttc tctccacagg tgtccactcc
     2641 caggtccaac tgcacctcgg ttctatcgaa aacgcgcctg ctagcgccac catgaagacc
     2701 atcattgccc tgagctacat cttctgcctg gtgttcgctg cggccgccat tgagattcct
     2761 agctctgtgc agcaggtccc caccatcatc aagcagagca aggtgcaggt cgcctttcca
     2821 ttcgacgagt acttccagat cgagtgcgag gccaagggca accctgagcc tacctttagc
     2881 tggaccaagg acggcaaccc cttctacttc accgaccaca gaatcatccc cagcaacaac
     2941 agcggcacct tcagaatccc caacgagggc cacatcagcc acttccaggg caagtacaga
     3001 tgcttcgcct ccaacaagct gggaatcgcc atgagcgaag agatcgagtt catcgtgccc
     3061 agcgtgccca agtttcccaa agagaagatc gaccctctcg aggtggaaga gggcgaccct
     3121 attgtgctgc cctgcaatcc tcctaagggc ctgcctcctc tgcacatcta ctggatgaac
     3181 atcgagctgg aacacatcga gcaggacgag cgggtgtaca tgagccagaa gggcgacctg
     3241 tacttcgcca acgtggaaga gaaggacagc cggaacgact actgctgctt cgccgccttt
     3301 cctagactgc ggaccatcgt gcagaaaatg cccatgaagc tgaccgtgaa cagcagcaac
     3361 tctatcaagc agcggaagcc caagctgctg ctgcctccta cagagagcgg aagcgagagc
     3421 agcatcacca tcctgaaggg cgagatcctg ctgctggaat gctttgccga gggactgcct
     3481 acacctcagg tggactggaa caagatcggc ggcgatctgc ctaagggcag agagacaaaa
     3541 gagaactacg gcaagaccct gaagatcgag aacgtgtcct accaggacaa gggcaattac
     3601 cggtgtaccg ccagcaactt cctgggcaca gccacacacg acttccacgt gatcgtggaa
     3661 gaacctcctc ggtggaccaa gaaacctcag agcgccgtgt acagcaccgg cagcaatgga
     3721 attctgctgt gcgaagccga gggcgagcct cagccaacaa tcaagtggcg cgtgaacggc
     3781 agccccgtgg acaatcatcc ttttgctggc gacgtggtgt tccccagaga gatcagcttc
     3841 accaacctgc agcctaacca cacagccgtg taccagtgtg aagccagcaa tgtgcacggc
     3901 acaatcctgg ccaacgccaa cattgacgtg gtggatgtgc ggcccctgat ccagacaaag
     3961 gacggcgaga attacgccac cgtcgtgggc tactctgcct tcctgcactg cgagttcttc
     4021 gcctctcctg aggctgtggt gtcctggcag aaagtggaag aagtgaagcc cctcgaaggc
     4081 cgcagatacc acatctacga gaacggcacc ctgcagatca accggaccac agaggaagat
     4141 gccggcagct actcttgctg ggtcgagaat gccatcggca agacagccgt gaccgccaac
     4201 ctggacatca gaaacgccac caagctgcgg gtgtccccaa agaaccccag aattcctaag
     4261 ctgcacatgc tggaactgca ttgcgagagc aagtgcgaca gccacctgaa gcacagcctg
     4321 aagctgtcct ggtccaagga tggcgaggcc tttgagatca acggcaccga ggatggccgg
     4381 atcatcatcg atggcgccaa tctgaccatc agcaacgtga ccctcgagga ccagggcatc
     4441 tactgttgta gcgcccacac tgccctggac tctgccgccg atatcaccca agtgaccgtg
     4501 ctggatgtcc ccgatcctcc tgagaatctg cacctgagcg agcggcagaa tagaagcgtc
     4561 agactgacat gggaggctgg cgccgaccac aacagcaaca tctccgagta catcgtggaa
     4621 ttcgagggca acaaagagga acccggccgt tgggaagaac tgaccagagt gcagggaaag
     4681 aaaaccaccg tgatcctgcc tctggctccc ttcgtgcgct accagtttag agtgatcgcc
     4741 gtgaacgaag tgggcagaag ccagccttct cagcccagcg atcaccacga aacacctcca
     4801 gccgctccag acagaaaccc tcagaacatc cgcgtgcagg ccagccagcc taaagaaatg
     4861 atcatcaagt gggagcccct gaagtccatg gaacagaatg gccctgggct cgagtacaga
     4921 gtgacctgga aacctcaagg cgcccctgtg gaatgggaag aagagacagt caccaatcac
     4981 accctgagag tgatgacccc tgccgtgtac gccccttacg atgtgaaagt gcaggctatc
     5041 aaccagctcg gcagcggccc agatcctcag tctgtgacac tgtacagcgg cgaggactac
     5101 cccgatacag cccctgtgat tcatggcgtg gacgtgatca acagcaccct ggtcaaagtg
     5161 acatggtcca cagtgcccaa ggacagagtg cacggtagac tgaagggcta ccagatcaac
     5221 tggtggaaaa caaagagcct gctggacggc cggacacacc ctaaagaagt caacatcctg
     5281 cggttcagcg gccagagaaa ctccggcatg gtgcctagcc tggatgcctt cagcgagttc
     5341 cacctgacag tgctggccta caactccaaa ggcgccggac ctgagagcga gccctacatc
     5401 tttcagacac ctgagggcgt gccagagcag cccacctttc tgaaagtgat caaggtggac
     5461 aaggacaccg ccacactgtc ttggggcctg ccaaagaagc tgaacggcaa cctgacaggc
     5521 tatctgctgc agtatcagat catcaacgac acctacgaga tcggcgagct gaacgacatc
     5581 aacatcacca cacctagcaa gcccagctgg cacctgtcca acctgaatgc caccaccaag
     5641 tacaagttct acctgcgggc ctgcaccagc caaggctgtg gaaagcctat caccgaggaa
     5701 agcagcacac tcggcgaggg ctctaaaggc atcggaaaga tcagcggcgt gaacctgaca
     5761 cagaaaacac accccatcga ggtgttcgag ccaggcgccg aacacattgt gcggctgatg
     5821 accaagaact ggggcgacaa cgacagcatc ttccaggatg tgatcgagac aagaggcaga
     5881 gagtacgccg gcctgtacga cgacatcagc acacaaggat ggggcgcgcc ggaagatcaa
     5941 gttgatccaa gattgattga tggcaagggc ggatctctgg aggtgctgtt ccagggacca
     6001 ggcggaagcg gcaatcccgg cggcgatctg gccccccaga tgctgcgcga gctgcaggag
     6061 accaatgccg ccctgcagga cgtgcgcgag ctgctgcgcc accgcgtgaa ggagatcacc
     6121 ttcctgaaga acaccgtgat ggagtgcgac gcctgcggta tgcagccctc tagaggctcc
     6181 ggctccatca tcccagttga ggaggagaac ccggacttct ggaaccgcga ggcagccgag
     6241 gccctgggtg ccgccaagaa gctgcagcct gcacagacag ccgccaagaa cctcatcatc
     6301 ttcctgggcg atgggatggg ggtgtctacg gtgacagctg ccaggatcct aaaagggcag
     6361 aagaaggaca aactggggcc tgagataccc ctggccatgg accgcttccc atatgtggct
     6421 ctgtccaaga catacaatgt agacaaacat gtgccagaca gtggagccac agccacggcc
     6481 tacctgtgcg gggtcaaggg caacttccag accattggct tgagtgcagc cgcccgcttt
     6541 aaccagtgca acacgacacg cggcaacgag gtcatctccg tgatgaatcg ggccaagaaa
     6601 gcagggaagt cagtgggagt ggtaaccacc acacgagtgc agcacgcctc gccagccggc
     6661 acctacgccc acacggtgaa ccgcaactgg tactcggacg ccgacgtgcc tgcctcggcc
     6721 cgccaggagg ggtgccagga catcgctacg cagctcatct ccaacatgga cattgacgtg
     6781 atcctaggtg gaggccgaaa gtacatgttt cgcatgggaa ccccagaccc tgagtaccca
     6841 gatgactaca gccaaggtgg gaccaggctg gacgggaaga atctggtgca ggaatggctg
     6901 gcgaagcgcc agggtgcccg gtatgtgtgg aaccgcactg agctcatgca ggcttccctg
     6961 gacccgtctg tgacccatct catgggtctc tttgagcctg gagacatgaa atacgagatc
     7021 caccgagact ccacactgga cccctccctg atggagatga cagaggctgc cctgcgcctg
     7081 ctgagcagga acccccgcgg cttcttcctc ttcgtggagg gtggtcgcat cgaccatggt
     7141 catcatgaaa gcagggctta ccgggcactg actgagacga tcatgttcga cgacgccatt
     7201 gagagggcgg gccagctcac cagcgaggag gacacgctga gcctcgtcac tgccgaccac
     7261 tcccacgtct tctccttcgg aggctacccc ctgcgaggga gctccatctt cgggctggcc
     7321 cctggcaagg cccgggacag gaaggcctac acggtcctcc tatacggaaa cggtccaggc
     7381 tatgtgctca aggacggcgc ccggccggat gttaccgaga gcgagagcgg gagccccgag
     7441 tatcggcagc agtcagcagt gcccctggac gaagagaccc acgcaggcga ggacgtggcg
     7501 gtgttcgcgc gcggcccgca ggcgcacctg gttcacggcg tgcaggagca gaccttcata
     7561 gcgcacgtca tggccttcgc cgcctgcctg gagccctaca ccgcctgcga cctggcgccc
     7621 cccgccggca ccaccgacgc cgcgcacccg ggtggcggag gactgaacga catcttcgag
     7681 gctcagaaaa tcgaatggca cgaacatcac catcaccatc accatcacca ttaaggccgg
     7741 cctgtagtca ctggcacaac gcgttacccg gtaagccaat cgggtataca cggtcgtcat
     7801 actgcagaca gggttcttct actttgcaag atagtcttga gtagtaaaat aaatagatag
     7861 agaaaatcag cctcgactgt gccttctagt tgccagccat ctgttgtttg cccctccccc
     7921 gtgccttcct tgaccctgga aggtgccact cccactgtcc tttcctaata aaatgaggaa
     7981 attgcatcac aacactcaac cctatctcgg tctattcttt tgatttataa gggattttgc
     8041 cgatttcggc ctattggtta aaaaatgagc tgatttaaca aaaatttaac gcgaattaat
     8101 tctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag caggcagaag
     8161 tatgcaaagc atgcatctca attagtcagc aaccaggtgt ggaaagtccc caggctcccc
     8221 agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccatag tcccgcccct
     8281 aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg
     8341 actaattttt tttatttatg cagaggccga ggccgcctct gcctctgagc tattccagaa
     8401 gtagtgagga ggcttttttg gaggcctagg cttttgcaaa aagctcccgg gagcttgtat
     8461 atccattttc ggatctgatc agcacgtgtt gacaattaat catcggcata gtatatcggc
     8521 atagtataat acgacaaggt gaggaactaa accatgaccg agtacaagcc cacggtgcgc
     8581 ctcgccaccc gcgacgacgt ccccagggcc gtacgcaccc tcgccgccgc gttcgccgac
     8641 taccccgcca cgcgccacac cgtcgatccg gaccgccaca tcgagcgggt caccgagctg
     8701 caagaactct tcctcacgcg cgtcgggctc gacatcggca aggtgtgggt cgcggacgac
     8761 ggcgccgcgg tggcggtctg gaccacgccg gagagcgtcg aagcgggggc ggtgttcgcc
     8821 gagatcggcc cgcgcatggc cgagttgagc ggttcccggc tggccgcgca gcaacagatg
     8881 gaaggcctcc tggcgccgca ccggcccaag gagcccgcgt ggttcctggc caccgtcggc
     8941 gtctcgcccg accaccaggg caagggtctg ggcagcgccg tcgtgctccc cggagtggag
     9001 gcggccgagc gcgccggggt gcccgccttc ctggagacat ccgcgccccg caacctcccc
     9061 ttctacgagc ggctcggctt caccgtcacc gccgacgtcg aggtgcccga aggaccgcgc
     9121 acctggtgca tgacccgcaa gcccggtgcc tgacacgtgc tacgagattt cgattccacc
     9181 gccgccttct atgaaaggtt gggcttcgga atcgttttcc gggacgccgg ctggatgatc
     9241 ctccagcgcg gggatctcat gctggagttc ttcgcccacc ccaacttgtt tattgcagct
     9301 tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc atttttttca
     9361 ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctgtataccg
     9421 tcgacctcta gctagagctt ggcgtaatca tggtcattac caatgcttaa tcagtgaggc
     9481 acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta
     9541 gataactacg atacgggagg gcttaccatc tggccccagc gctgcgatga taccgcgaga
     9601 accacgctca ccggctccgg atttatcagc aataaaccag ccagccggaa gggccgagcg
     9661 cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc
     9721 tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccatcg ctacaggcat
     9781 cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag
     9841 gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat
     9901 cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa
     9961 ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa
    10021 gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga
    10081 taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg
    10141 gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc
    10201 acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg
    10261 aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatatt
    10321 cttcctt
//
