LOCUS       sequence-304303-       10582 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of cell-surface protein extracellular domain 
            fused to COMP5AP-AviTag-9xHis. Protein is secreted from cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10582)
  AUTHORS   Wojtowicz WM, Vielmetter J, Fernandes RA, Siepe DH, Eastman CL, 
            Chisholm GB, Cox S, Klock H, Anderson PW, Rue SM, Miller JJ, Glaser 
            SM, Bragstad ML, Vance J, Lam AW, Lesley SA, Zinn K, Garcia KC
  TITLE     A Human IgSF Cell-Surface Interactome Reveals a Complex Network of 
            Protein-Protein Interactions.
  JOURNAL   Cell. 2020 Aug 20;182(4):1027-1043.e17. doi: 
            10.1016/j.cell.2020.07.025.
  PUBMED    32822567
REFERENCE   2  (bases 1 to 10582)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2020.07"; journalName: "Cell"; date: "2020-08-20- 
            20"; volume: "182"; issue: "4"; pages: "1027-1043.e17"
COMMENT     Sequence Label: pD649-HAsp-NRCAM-COMP5AP-AviTag-9xHis
FEATURES             Location/Qualifiers
     source          1..10582
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     124..143
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     149..168
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     rep_origin      254..842
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     743..762
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     996..1013
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     1111..1128
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     1112..1128
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    1584..1600
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     enhancer        1767..2146
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2147..2350
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2301..2321
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     2347..2371
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        2581..2599
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     2581..2598
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     2612..2631
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     CDS             6187..6222
                     /codon_start=1
                     /product="epitope tag derived from human Protein C (Stearns
                     et al., 1988)"
                     /label=Protein C tag
                     /translation="EDQVDPRLIDGK"
     CDS             6232..6255
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     CDS             7915..7959
                     /codon_start=1
                     /product="peptide tag that allows for enzymatic 
                     biotinylation"
                     /label=AviTag(TM)
                     /translation="GLNDIFEAQKIEWHE"
     CDS             7960..7986
                     /codon_start=1
                     /product="9xHis affinity tag"
                     /label=9xHis
                     /translation="HHHHHHHHH"
     primer_bind     complement(8126..8143)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    8132..8243
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(8361..8381)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        8366..8695
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      8546..8681
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     8608..8627
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        8743..8790
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             8809..9408
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(8809..8828)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     9305..9325
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     polyA_signal    9538..9659
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9575..9594)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9629..9648
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     CDS             complement(join(9712..10503,10504..10572))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10335..10354
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(join(10573..10582,1..82))
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa
       61 tgtatttaga aaaataaaca aataggggtc agtgttacaa ccaattaacc aattctgaac
      121 attatcgcga gcccatttat acctgaatat ggctcataac accccttgct catgaccaaa
      181 atcccttaac gtgagttacg cgcgcgtcgt tccactgagc gtcagacccc gtagaaaaga
      241 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa
      301 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga
      361 aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt
      421 tagcccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt
      481 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat
      541 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct
      601 tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca
      661 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
      721 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
      781 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
      841 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
      901 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
      961 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg
     1021 aaggcgagag tagggaactg ccaggcatca aactaagcag aaggcccctg acggatggcc
     1081 tttttgcgtt tctacaaact ctttctgtgt tgtaaaacga cggccagtct taagctcggg
     1141 ccccctgggc ggttctgata acgagtaatc gttaatccgc aaataacgta aaaacccgct
     1201 tcggcgggtt tttttatggg gggagtttag ggaaagagca tttgtcagaa tatttaaggg
     1261 cgcctgtcac tttgcttgat atatgagaat tatttaacct tataaatgag aaaaaagcaa
     1321 cgcactttaa ataagatacg ttgctttttc gattgatgaa cacctataat taaactattc
     1381 atctattatt tatgattttt tgtatataca atatttctag tttgttaaag agaattaaga
     1441 aaataaatct cgaaaataat aaagggaaaa tcagtttttg atatcaaaat tatacatgtc
     1501 aacgataata caaaatataa tacaaactat aagatgttat cagtatttat tatcatttag
     1561 aataaatttt gtgtcgccct taattgtgag cggataacaa ttacgagctt catgcacagt
     1621 ggcgttgact caatattggc cattagccat attattcatt ggttatatag cataaatcaa
     1681 tattggctat tggccattgc atacgttgta tctatatcat aatatgtaca tttatattgg
     1741 ctcatgtcca atatgaccgc catgttggca ttgattattg actagttatt aatagtaatc
     1801 aattacgggg tcattagttc atagcccata tatggagttc cgcgttacat aacttacggt
     1861 aaatggcccg cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta
     1921 tgttcccata gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg
     1981 gtaaactgcc cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga
     2041 cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag tacatgacct tatgggactt
     2101 tcctacttgg cagtacatct acgtattagt catcgctatt accatggtga tgcggttttg
     2161 gcagtacatc aatgggcgtg gatagcggtt tgactcacgg ggatttccaa gtctccaccc
     2221 cattgacgtc aatgggagtt tgttttggca ccaaaatcaa cgggactttc caaaatgtcg
     2281 taacaactcc gccccattga cgcaaatggg cggtaggcgt gtacggtggg aggtctatat
     2341 aagcagagct cgtttagtga accgtcagat cgcctggaga cgccatccac gctgttttga
     2401 cctccataga agacaccggg accgatccag cctccgcggc cgggaacggt gcattggaac
     2461 gcggattccc cgtgccaaga gtgacgtaag taccgcctat agagtctata ggcccacccc
     2521 cttggcttcg ttagaacgcg gctacaatta atacataacc ttatgtatca tacacatacg
     2581 atttaggtga cactatagaa taacatccac tttgcctttc tctccacagg tgtccactcc
     2641 caggtccaac tgcacctcgg ttctatcgaa aacgcgcctg ctagcgccac catgaagacc
     2701 atcattgccc tgagctacat cttctgcctg gtgttcgctg cggccgccca aatgatttct
     2761 gccctggaag tgcctctgga ccccaagctg ctggaagatc tggtgcagcc tcctaccatc
     2821 acacagcaga gccccaagga ctacatcatc gaccccagag aaaacatcgt gatccagtgc
     2881 gaggccaagg gcaagcctcc tccaagcttt agctggacca gaaacggcac ccacttcgat
     2941 attgacaagg accctctggt caccatgaag cccggaaccg gcacactgat catcaacatc
     3001 atgagcgagg gcaaagccga gacatacgag ggcgtgtacc agtgcaccgc cagaaatgaa
     3061 cggggagccg ccgtgtccaa caacatcgtc gttagaccca gcagatcccc tctgtggacc
     3121 aaagagaagc tggaacccat cactctgcag tccggccagt ctctggtgct gccttgtaga
     3181 cctcctatcg gcctgcctcc tcctatcatc ttctggatgg acaacagctt ccagcggctg
     3241 ccccagagcg aaagagttag ccaaggcctg aacggcgacc tgtacttcag caacgtgctg
     3301 cccgaggaca ccagagagga ttacatctgc tacgcccggt tcaatcacac ccagaccatc
     3361 cagcagaaac agcccatcag cgtgaaagtg atcagcgtgg acgagctgaa cgacacaatc
     3421 gccgccaacc tgagcgacac cgagttttac ggcgccaaga gcagcagaga gcggcctcca
     3481 acatttctga cccctgaggg caacgccagc aacaaagagg aactgcgggg aaacgtgctg
     3541 agcctggaat gtattgccga gggcctgcct acaccaatta tctactgggc caaagaggac
     3601 ggcatgctgc ccaagaaccg gaccgtgtac aagaacttcg agaaaaccct gcagatcatc
     3661 cacgtgtccg aggccgacag cggcaactat cagtgtatcg ccaagaatgc cctgggcgcc
     3721 atccaccaca caatcagcgt tagagtgaag gccgctcctt attggatcac agcccctcag
     3781 aacctggtgc tgtctcctgg cgaagatggc accctgatct gtagagccaa cggcaacccc
     3841 aagcctcgga tcagctggct gacaaatggc gtgcccatcg agatcgcccc tgacgaccct
     3901 agcagaaaga tcgacggcga caccatcatc ttttccaacg tgcaagagcg gagcagcgcc
     3961 gtctaccagt gtaatgccag caacgagtac ggctacctgc tggccaacgc cttcgtgaat
     4021 gtgctggccg agcctcctag aatcctgaca ccagccaaca cactgtacca agtgatcgcc
     4081 aacagacccg ctctgctgga ctgcgccttt tttggctctc ccctgcctac catcgagtgg
     4141 ttcaaaggcg ccaagggatc tgccctgcac gaggacatct atgtgctgca cgagaacgga
     4201 accctggaaa tccccgtggc tcagaaggat agcaccggca cctatacctg tgtggccaga
     4261 aacaagctcg gcatggccaa gaacgaagtg cacctcgaga tcaaggaccc cacctggatc
     4321 gtgaagcagc ctgagtatgc tgtggtgcag aggggctcca tggtgtcctt cgagtgcaaa
     4381 gtgaagcacg atcacaccct gagcctgacc gtgctgtggc tgaaggacaa tagagagctg
     4441 cccagcgacg agcggttcac cgtggataag gatcatctgg tggtggccga tgtgtccgac
     4501 gatgactctg gcacctacac atgcgtggca aacaccacac tggacagcgt gtcagcttcc
     4561 gccgtgctgt ctgtggttgc ccctacacct acaccagctc cagtgtacga cgtgcccaat
     4621 cctcctttcg acctggaact gaccgaccag ctggacaaga gcgtgcagct gtcttggaca
     4681 cccggcgacg acaacaacag ccccatcacc aagttcatca tcgagtacga ggacgccatg
     4741 cacaagcctg gactgtggca ccatcagacc gaagtgtctg gcacccagac aactgcccag
     4801 ctgaagctga gcccctacgt gaactactcc ttccgcgtga tggccgtgaa cagcatcggc
     4861 aagtctctgc cttctgaggc cagcgagcag tacctgacaa aggcctccga gcctgacaag
     4921 aaccctacag ccgttgaagg cctgggcagc gagcctgata atctggtcat cacctggaag
     4981 cccctgaatg gcttcgagag caatggccct ggcctgcagt ataaggtttc ctggcgacag
     5041 aaggatggcg acgacgagtg gacatctgtg gtggtcgcta acgtgtccaa gtatatcgtg
     5101 tccggcacac ccaccttcgt gccctacctg attaaggtgc aggccctgaa cgatatgggc
     5161 ttcgctcctg aacctgccgt ggtcatggga cacagcggag aggatctgcc tatggtggct
     5221 cctggcaatg tgcgcgtgaa cgtggtcaat agcacactgg ccgaagtgca ttgggacccc
     5281 gtgcctctga agtccatcag aggccatctc cagggctaca gaatctacta ctggaaaacc
     5341 cagagcagct ccaagcggaa cagacggcac atcgagaaga agatcctgac atttcagggc
     5401 agcaagaccc acggaatgct gcctggcctg gaacctttca gccactacac cctgaatgtg
     5461 cgggtcgtga acggcaaagg cgagggacct gcttctcccg acagagtgtt caatacccca
     5521 gagggcgtgc caagcgctcc ttcctctctg aaaatcgtga accctactct ggacagcctg
     5581 acactggaat gggaccctcc atctcacccc aacggcatcc tgaccgagta cacactgaag
     5641 taccagccta tcaacagcac ccacgagctg ggccctctgg tggatctgaa gatccctgcc
     5701 aacaagacca ggtggaccct gaagaatctg aacttcagca cccggtacaa gttctacttc
     5761 tacgcccaga caagcgccgg cagcggaagc cagattactg aagaggccgt gaccacagtg
     5821 gacgaggccg gaattctgcc tcctgatgtt ggagccggca aagtgcaggc tgtgaacccc
     5881 agaatcagca acctgacagc cgccgctgcc gaaacctacg ccaatatcag ctgggagtat
     5941 gagggccccg agcacgtgaa cttctacgtg gaatatggcg tggccggcag caaagaagag
     6001 tggcggaaag aaattgtgaa cggctctcgg agcttcttcg gcctgaaggg actgatgcct
     6061 ggcaccgcct acaaagttcg agtgggagcc gtgggcgata gcggctttgt gtctagcgag
     6121 gacgtgttcg agacaggccc tgccatggct tccagacagg tggacattgc cacacaaggc
     6181 gcgccggaag atcaagttga tccaagattg attgatggca agggcggatc tctggaggtg
     6241 ctgttccagg gaccaggcgg aagcggcaat cccggcggcg atctggcccc ccagatgctg
     6301 cgcgagctgc aggagaccaa tgccgccctg caggacgtgc gcgagctgct gcgccaccgc
     6361 gtgaaggaga tcaccttcct gaagaacacc gtgatggagt gcgacgcctg cggtatgcag
     6421 ccctctagag gctccggctc catcatccca gttgaggagg agaacccgga cttctggaac
     6481 cgcgaggcag ccgaggccct gggtgccgcc aagaagctgc agcctgcaca gacagccgcc
     6541 aagaacctca tcatcttcct gggcgatggg atgggggtgt ctacggtgac agctgccagg
     6601 atcctaaaag ggcagaagaa ggacaaactg gggcctgaga tacccctggc catggaccgc
     6661 ttcccatatg tggctctgtc caagacatac aatgtagaca aacatgtgcc agacagtgga
     6721 gccacagcca cggcctacct gtgcggggtc aagggcaact tccagaccat tggcttgagt
     6781 gcagccgccc gctttaacca gtgcaacacg acacgcggca acgaggtcat ctccgtgatg
     6841 aatcgggcca agaaagcagg gaagtcagtg ggagtggtaa ccaccacacg agtgcagcac
     6901 gcctcgccag ccggcaccta cgcccacacg gtgaaccgca actggtactc ggacgccgac
     6961 gtgcctgcct cggcccgcca ggaggggtgc caggacatcg ctacgcagct catctccaac
     7021 atggacattg acgtgatcct aggtggaggc cgaaagtaca tgtttcgcat gggaacccca
     7081 gaccctgagt acccagatga ctacagccaa ggtgggacca ggctggacgg gaagaatctg
     7141 gtgcaggaat ggctggcgaa gcgccagggt gcccggtatg tgtggaaccg cactgagctc
     7201 atgcaggctt ccctggaccc gtctgtgacc catctcatgg gtctctttga gcctggagac
     7261 atgaaatacg agatccaccg agactccaca ctggacccct ccctgatgga gatgacagag
     7321 gctgccctgc gcctgctgag caggaacccc cgcggcttct tcctcttcgt ggagggtggt
     7381 cgcatcgacc atggtcatca tgaaagcagg gcttaccggg cactgactga gacgatcatg
     7441 ttcgacgacg ccattgagag ggcgggccag ctcaccagcg aggaggacac gctgagcctc
     7501 gtcactgccg accactccca cgtcttctcc ttcggaggct accccctgcg agggagctcc
     7561 atcttcgggc tggcccctgg caaggcccgg gacaggaagg cctacacggt cctcctatac
     7621 ggaaacggtc caggctatgt gctcaaggac ggcgcccggc cggatgttac cgagagcgag
     7681 agcgggagcc ccgagtatcg gcagcagtca gcagtgcccc tggacgaaga gacccacgca
     7741 ggcgaggacg tggcggtgtt cgcgcgcggc ccgcaggcgc acctggttca cggcgtgcag
     7801 gagcagacct tcatagcgca cgtcatggcc ttcgccgcct gcctggagcc ctacaccgcc
     7861 tgcgacctgg cgccccccgc cggcaccacc gacgccgcgc acccgggtgg cggaggactg
     7921 aacgacatct tcgaggctca gaaaatcgaa tggcacgaac atcaccatca ccatcaccat
     7981 caccattaag gccggcctgt agtcactggc acaacgcgtt acccggtaag ccaatcgggt
     8041 atacacggtc gtcatactgc agacagggtt cttctacttt gcaagatagt cttgagtagt
     8101 aaaataaata gatagagaaa atcagcctcg actgtgcctt ctagttgcca gccatctgtt
     8161 gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg ccactcccac tgtcctttcc
     8221 taataaaatg aggaaattgc atcacaacac tcaaccctat ctcggtctat tcttttgatt
     8281 tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat
     8341 ttaacgcgaa ttaattctgt ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc
     8401 cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca ggtgtggaaa
     8461 gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac
     8521 catagtcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc
     8581 tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg cctctgcctc
     8641 tgagctattc cagaagtagt gaggaggctt ttttggaggc ctaggctttt gcaaaaagct
     8701 cccgggagct tgtatatcca ttttcggatc tgatcagcac gtgttgacaa ttaatcatcg
     8761 gcatagtata tcggcatagt ataatacgac aaggtgagga actaaaccat gaccgagtac
     8821 aagcccacgg tgcgcctcgc cacccgcgac gacgtcccca gggccgtacg caccctcgcc
     8881 gccgcgttcg ccgactaccc cgccacgcgc cacaccgtcg atccggaccg ccacatcgag
     8941 cgggtcaccg agctgcaaga actcttcctc acgcgcgtcg ggctcgacat cggcaaggtg
     9001 tgggtcgcgg acgacggcgc cgcggtggcg gtctggacca cgccggagag cgtcgaagcg
     9061 ggggcggtgt tcgccgagat cggcccgcgc atggccgagt tgagcggttc ccggctggcc
     9121 gcgcagcaac agatggaagg cctcctggcg ccgcaccggc ccaaggagcc cgcgtggttc
     9181 ctggccaccg tcggcgtctc gcccgaccac cagggcaagg gtctgggcag cgccgtcgtg
     9241 ctccccggag tggaggcggc cgagcgcgcc ggggtgcccg ccttcctgga gacatccgcg
     9301 ccccgcaacc tccccttcta cgagcggctc ggcttcaccg tcaccgccga cgtcgaggtg
     9361 cccgaaggac cgcgcacctg gtgcatgacc cgcaagcccg gtgcctgaca cgtgctacga
     9421 gatttcgatt ccaccgccgc cttctatgaa aggttgggct tcggaatcgt tttccgggac
     9481 gccggctgga tgatcctcca gcgcggggat ctcatgctgg agttcttcgc ccaccccaac
     9541 ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat
     9601 aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat
     9661 catgtctgta taccgtcgac ctctagctag agcttggcgt aatcatggtc attaccaatg
     9721 cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg
     9781 actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagcgctgc
     9841 gatgataccg cgagaaccac gctcaccggc tccggattta tcagcaataa accagccagc
     9901 cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa
     9961 ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc
    10021 catcgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg
    10081 ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc
    10141 cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat
    10201 ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg
    10261 tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc
    10321 ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg
    10381 aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat
    10441 gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg
    10501 gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg
    10561 ttgaatactc atattcttcc tt
//
