LOCUS       sequence-304305-       10702 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of cell-surface protein partial extracellular 
            domain fused to COMP5AP-AviTag-9xHis. Protein is secreted from 
            cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10702)
  AUTHORS   Wojtowicz WM, Vielmetter J, Fernandes RA, Siepe DH, Eastman CL, 
            Chisholm GB, Cox S, Klock H, Anderson PW, Rue SM, Miller JJ, Glaser 
            SM, Bragstad ML, Vance J, Lam AW, Lesley SA, Zinn K, Garcia KC
  TITLE     A Human IgSF Cell-Surface Interactome Reveals a Complex Network of 
            Protein-Protein Interactions.
  JOURNAL   Cell. 2020 Aug 20;182(4):1027-1043.e17. doi: 
            10.1016/j.cell.2020.07.025.
  PUBMED    32822567
REFERENCE   2  (bases 1 to 10702)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2020.07"; journalName: "Cell"; date: "2020-08-20- 
            20"; volume: "182"; issue: "4"; pages: "1027-1043.e17"
COMMENT     Sequence Label: pD649-HAsp-MXRA5(Trunc2)-COMP5AP-AviTag-9xHis
FEATURES             Location/Qualifiers
     source          1..10702
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     124..143
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     149..168
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     rep_origin      254..842
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     743..762
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     996..1013
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     1111..1128
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     1112..1128
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    1584..1600
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     enhancer        1767..2146
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2147..2350
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2301..2321
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     2347..2371
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        2581..2599
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     2581..2598
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     2612..2631
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     CDS             6307..6342
                     /codon_start=1
                     /product="epitope tag derived from human Protein C (Stearns
                     et al., 1988)"
                     /label=Protein C tag
                     /translation="EDQVDPRLIDGK"
     CDS             6352..6375
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     CDS             8035..8079
                     /codon_start=1
                     /product="peptide tag that allows for enzymatic 
                     biotinylation"
                     /label=AviTag(TM)
                     /translation="GLNDIFEAQKIEWHE"
     CDS             8080..8106
                     /codon_start=1
                     /product="9xHis affinity tag"
                     /label=9xHis
                     /translation="HHHHHHHHH"
     primer_bind     complement(8246..8263)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    8252..8363
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(8481..8501)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        8486..8815
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      8666..8801
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        8863..8910
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             8929..9528
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(8929..8948)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     9425..9445
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     polyA_signal    9658..9779
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9695..9714)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9749..9768
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     CDS             complement(join(9832..10623,10624..10692))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10455..10474
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(join(10693..10702,1..82))
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa
       61 tgtatttaga aaaataaaca aataggggtc agtgttacaa ccaattaacc aattctgaac
      121 attatcgcga gcccatttat acctgaatat ggctcataac accccttgct catgaccaaa
      181 atcccttaac gtgagttacg cgcgcgtcgt tccactgagc gtcagacccc gtagaaaaga
      241 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa
      301 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga
      361 aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt
      421 tagcccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt
      481 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat
      541 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct
      601 tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca
      661 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
      721 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
      781 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
      841 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
      901 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
      961 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg
     1021 aaggcgagag tagggaactg ccaggcatca aactaagcag aaggcccctg acggatggcc
     1081 tttttgcgtt tctacaaact ctttctgtgt tgtaaaacga cggccagtct taagctcggg
     1141 ccccctgggc ggttctgata acgagtaatc gttaatccgc aaataacgta aaaacccgct
     1201 tcggcgggtt tttttatggg gggagtttag ggaaagagca tttgtcagaa tatttaaggg
     1261 cgcctgtcac tttgcttgat atatgagaat tatttaacct tataaatgag aaaaaagcaa
     1321 cgcactttaa ataagatacg ttgctttttc gattgatgaa cacctataat taaactattc
     1381 atctattatt tatgattttt tgtatataca atatttctag tttgttaaag agaattaaga
     1441 aaataaatct cgaaaataat aaagggaaaa tcagtttttg atatcaaaat tatacatgtc
     1501 aacgataata caaaatataa tacaaactat aagatgttat cagtatttat tatcatttag
     1561 aataaatttt gtgtcgccct taattgtgag cggataacaa ttacgagctt catgcacagt
     1621 ggcgttgact caatattggc cattagccat attattcatt ggttatatag cataaatcaa
     1681 tattggctat tggccattgc atacgttgta tctatatcat aatatgtaca tttatattgg
     1741 ctcatgtcca atatgaccgc catgttggca ttgattattg actagttatt aatagtaatc
     1801 aattacgggg tcattagttc atagcccata tatggagttc cgcgttacat aacttacggt
     1861 aaatggcccg cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta
     1921 tgttcccata gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg
     1981 gtaaactgcc cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga
     2041 cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag tacatgacct tatgggactt
     2101 tcctacttgg cagtacatct acgtattagt catcgctatt accatggtga tgcggttttg
     2161 gcagtacatc aatgggcgtg gatagcggtt tgactcacgg ggatttccaa gtctccaccc
     2221 cattgacgtc aatgggagtt tgttttggca ccaaaatcaa cgggactttc caaaatgtcg
     2281 taacaactcc gccccattga cgcaaatggg cggtaggcgt gtacggtggg aggtctatat
     2341 aagcagagct cgtttagtga accgtcagat cgcctggaga cgccatccac gctgttttga
     2401 cctccataga agacaccggg accgatccag cctccgcggc cgggaacggt gcattggaac
     2461 gcggattccc cgtgccaaga gtgacgtaag taccgcctat agagtctata ggcccacccc
     2521 cttggcttcg ttagaacgcg gctacaatta atacataacc ttatgtatca tacacatacg
     2581 atttaggtga cactatagaa taacatccac tttgcctttc tctccacagg tgtccactcc
     2641 caggtccaac tgcacctcgg ttctatcgaa aacgcgcctg ctagcgccac catgaagacc
     2701 atcattgccc tgagctacat cttctgcctg gtgttcgctg cggccgccaa aaaaggctct
     2761 ggcctgcctt ctaagagagg cagaaggcct ggcgccaaag ctctgagtag agtgcgggaa
     2821 gatatcgtgg aagatgaagg cggctctggc atgggcgacg aggaaaacac aagcagaagg
     2881 ctgctgcacc ccaaggacca agaggtgttc ctgaaaacaa aggacgacgc catcaacggc
     2941 gacaagaagg ccaagaaagg cagacggaag ctgaagctgt ggaagcacag cgagaaagaa
     3001 cccgagacaa acgtggccga gggcagaaga gtgtttgaga gccggcggag aatcaacatg
     3061 gccaacaagc agatcaaccc cgagagatgg gccgacattc tggccaaagt gcggggcaag
     3121 aatctgccta agggaaccga agtgcctcct ctgatcaaga ccacctctcc acctagcctg
     3181 agcctggaag tgacccctcc ttttcctgcc atctcacctc caagcgcctc tccagtgcag
     3241 acagtgacaa gcgccgagga atcttctgcc gatgtgcctc tgctgggaga agaggaacac
     3301 gtgctgggaa caatcagcag cgcctctatg ggcctcgagc acaaccataa tggcgtgatc
     3361 ctggtggaac ccgaagtgac cagcacacct ctggaagagg tggtggacga cctgagcgag
     3421 aaaaccgagg aaatcaccag caccgagggc gaccttaagg gaacagccgc tcctacactg
     3481 atcagcgagc cttacgagcc ctctccaaca ctgcacaccc tggacacagt gtacgagaag
     3541 cccacacacg aggaaacagc cactgaaggc tggtccgctg cagatgtggg aagctctcct
     3601 gagcctacaa gcagcgagta cgaacctcct ctggatgccg tgtctctggc cgagtctgag
     3661 cccatgcagt acttcgaccc cgacctggaa acaaagagcc agcctgacga ggacaaaatg
     3721 aaggaagata cgttcgccca cctgacgcct acacctacca tctgggtcaa cgatagcagc
     3781 accagccagc tgttcgagga tagcacaatt ggagagcctg gcgtgccagg acagtctcat
     3841 ctgcaaggcc tgaccgacaa catccacctg gtcaagagca gcctgtctac ccaggacacc
     3901 ctgctgatca aaaagggcat gaaggaaatg agccagacac tgcaaggcgg caacatgctg
     3961 gaaggcgacc ctacacacag cagatctagc gagagcgagg gccaagagag caagagcatc
     4021 accctgcctg atagcaccct gggcatcatg agcagcatga gccctgtgaa gaagcccgcc
     4081 gagacaacag tgggcacact gctggataag gataccacca ccgccaccac cacacctaga
     4141 cagaaagtgg cccctagctc caccatgagc acacacccta gcagaagaag gcctaacgga
     4201 aggcggagac tgcggcccaa caagttcaga cacagacaca aacagacccc tccaaccaca
     4261 ttcgccccta gcgagacatt cagcacccag cctacacagg cccctgacat caagatcagc
     4321 agccaggtgg aaagcagcct ggtgcctaca gcctgggtcg acaataccgt gaacacccct
     4381 aagcagctcg agatggaaaa gaacgccgag cctaccagca agggcacccc tagaagaaag
     4441 cacggcaagc ggcctaacaa gcacagatac acccctagca ccgtgtctag cagagccagc
     4501 ggaagcaagc cttctccatc tcctgagaac aagcaccgga acatcgtgac cccttccagc
     4561 gagacaatcc tgctgcctcg gacagtgtct ctgaaaacag agggccccta cgacagcctg
     4621 gactacatga ccaccaccag aaagatctac agcagctacc ccaaggtgca agagactctg
     4681 cccgtgacct acaagccaac cagcgacggc aaagaaatca aggacgatgt ggccaccaac
     4741 gtggacaagc acaagagcga tatcctggtc accggcgagt ccatcaccaa cgccattcca
     4801 acctccagat ctctggtgtc caccatgggc gagttcaaag aagagagcag cccagtcggc
     4861 ttccccggca cacctacatg gaatccaagc agaacagccc agcctggcag actgcagaca
     4921 ggcatccctg tgacaaccag cggcgagaat ctgaccgatc ctccactgct gaaagaactc
     4981 gaggacgtgg acttcaccag cgagtttctg tcctctctga ccgtgtccac acctttccac
     5041 caagaggaag ccggcagcag cacaaccctg tcctctatta aggtggaagt ggcctccagc
     5101 caggccgaga ctacaacact ggatcaggac catctggaaa ccaccgtggc catcctgctg
     5161 agcgaaacca gacctcagaa tcacacccct accgccgcta ggatgaagga acctgccagc
     5221 agctctccct ccaccatcct gatgtctctg ggccagacca caaccaccaa gcctgctctc
     5281 ccatctccaa gaatctccca ggccagccgg gacagcaaag aaaatgtgtt tctgaactac
     5341 gtgggcaacc ccgaaaccga ggccacacct gtgaacaatg agggcacaca gcacatgagc
     5401 ggccccaatg agctgagcac acctagcagc gaccaggacg ccttcaatct gagcaccaag
     5461 ctggaactgg aaaaacaggt gttcggcagc agaagcctgc ctagaggccc tgactctcag
     5521 agacaggatg gaagagtgca cgccagccac cagcttacca gagtgcctgc caaacctatc
     5581 ctgccaacag ctacagtgcg gctgcctgag atgtctacac agagcgccag ccggtacttc
     5641 gtgacctctc agtctcctcg gcactggacc aacaagcccg agatcacaac ataccctagc
     5701 ggagccctgc cagagaacaa acagttcaca acccctcggc tgtccagcac cacaattcct
     5761 ctgcctctgc acatgtccaa gcctagcatc cccagcaagt tcaccgacag acggaccgac
     5821 cagttcaacg gctacagcaa agtgttcgga aacaacaaca tccccgaggc cagaaatccc
     5881 gtgggcaagc caccttctcc tagaatccct cactacagca acggccggct gccattcttc
     5941 accaacaaga ccctgagctt ccctcagctg ggagtgacca gaaggccaca gatccctaca
     6001 tctcccgctc cagtgatgcg cgagagaaaa gtgatccccg gcagctacaa cagaatccac
     6061 agccacagca cctttcacct ggactttgga cctccagctc ctcctctgct gcacacacct
     6121 cagacaacag gcagcccaag caccaacctg cagaacatcc ctatggtgtc ctccacacag
     6181 agcagcatca gcttcatcac cagctctgtg cagagcagcg gctcctttca ccagagcagc
     6241 tccaagtttt ttgccggcgg acctcctgcc tccaagttct ggtccctggg agagaaaggc
     6301 gcgccggaag atcaagttga tccaagattg attgatggca agggcggatc tctggaggtg
     6361 ctgttccagg gaccaggcgg aagcggcaat cccggcggcg atctggcccc ccagatgctg
     6421 cgcgagctgc aggagaccaa tgccgccctg caggacgtgc gcgagctgct gcgccaccgc
     6481 gtgaaggaga tcaccttcct gaagaacacc gtgatggagt gcgacgcctg cggtatgcag
     6541 ccctctagag gctccggctc catcatccca gttgaggagg agaacccgga cttctggaac
     6601 cgcgaggcag ccgaggccct gggtgccgcc aagaagctgc agcctgcaca gacagccgcc
     6661 aagaacctca tcatcttcct gggcgatggg atgggggtgt ctacggtgac agctgccagg
     6721 atcctaaaag ggcagaagaa ggacaaactg gggcctgaga tacccctggc catggaccgc
     6781 ttcccatatg tggctctgtc caagacatac aatgtagaca aacatgtgcc agacagtgga
     6841 gccacagcca cggcctacct gtgcggggtc aagggcaact tccagaccat tggcttgagt
     6901 gcagccgccc gctttaacca gtgcaacacg acacgcggca acgaggtcat ctccgtgatg
     6961 aatcgggcca agaaagcagg gaagtcagtg ggagtggtaa ccaccacacg agtgcagcac
     7021 gcctcgccag ccggcaccta cgcccacacg gtgaaccgca actggtactc ggacgccgac
     7081 gtgcctgcct cggcccgcca ggaggggtgc caggacatcg ctacgcagct catctccaac
     7141 atggacattg acgtgatcct aggtggaggc cgaaagtaca tgtttcgcat gggaacccca
     7201 gaccctgagt acccagatga ctacagccaa ggtgggacca ggctggacgg gaagaatctg
     7261 gtgcaggaat ggctggcgaa gcgccagggt gcccggtatg tgtggaaccg cactgagctc
     7321 atgcaggctt ccctggaccc gtctgtgacc catctcatgg gtctctttga gcctggagac
     7381 atgaaatacg agatccaccg agactccaca ctggacccct ccctgatgga gatgacagag
     7441 gctgccctgc gcctgctgag caggaacccc cgcggcttct tcctcttcgt ggagggtggt
     7501 cgcatcgacc atggtcatca tgaaagcagg gcttaccggg cactgactga gacgatcatg
     7561 ttcgacgacg ccattgagag ggcgggccag ctcaccagcg aggaggacac gctgagcctc
     7621 gtcactgccg accactccca cgtcttctcc ttcggaggct accccctgcg agggagctcc
     7681 atcttcgggc tggcccctgg caaggcccgg gacaggaagg cctacacggt cctcctatac
     7741 ggaaacggtc caggctatgt gctcaaggac ggcgcccggc cggatgttac cgagagcgag
     7801 agcgggagcc ccgagtatcg gcagcagtca gcagtgcccc tggacgaaga gacccacgca
     7861 ggcgaggacg tggcggtgtt cgcgcgcggc ccgcaggcgc acctggttca cggcgtgcag
     7921 gagcagacct tcatagcgca cgtcatggcc ttcgccgcct gcctggagcc ctacaccgcc
     7981 tgcgacctgg cgccccccgc cggcaccacc gacgccgcgc acccgggtgg cggaggactg
     8041 aacgacatct tcgaggctca gaaaatcgaa tggcacgaac atcaccatca ccatcaccat
     8101 caccattaag gccggcctgt agtcactggc acaacgcgtt acccggtaag ccaatcgggt
     8161 atacacggtc gtcatactgc agacagggtt cttctacttt gcaagatagt cttgagtagt
     8221 aaaataaata gatagagaaa atcagcctcg actgtgcctt ctagttgcca gccatctgtt
     8281 gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg ccactcccac tgtcctttcc
     8341 taataaaatg aggaaattgc atcacaacac tcaaccctat ctcggtctat tcttttgatt
     8401 tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat
     8461 ttaacgcgaa ttaattctgt ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc
     8521 cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca ggtgtggaaa
     8581 gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac
     8641 catagtcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc
     8701 tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg cctctgcctc
     8761 tgagctattc cagaagtagt gaggaggctt ttttggaggc ctaggctttt gcaaaaagct
     8821 cccgggagct tgtatatcca ttttcggatc tgatcagcac gtgttgacaa ttaatcatcg
     8881 gcatagtata tcggcatagt ataatacgac aaggtgagga actaaaccat gaccgagtac
     8941 aagcccacgg tgcgcctcgc cacccgcgac gacgtcccca gggccgtacg caccctcgcc
     9001 gccgcgttcg ccgactaccc cgccacgcgc cacaccgtcg atccggaccg ccacatcgag
     9061 cgggtcaccg agctgcaaga actcttcctc acgcgcgtcg ggctcgacat cggcaaggtg
     9121 tgggtcgcgg acgacggcgc cgcggtggcg gtctggacca cgccggagag cgtcgaagcg
     9181 ggggcggtgt tcgccgagat cggcccgcgc atggccgagt tgagcggttc ccggctggcc
     9241 gcgcagcaac agatggaagg cctcctggcg ccgcaccggc ccaaggagcc cgcgtggttc
     9301 ctggccaccg tcggcgtctc gcccgaccac cagggcaagg gtctgggcag cgccgtcgtg
     9361 ctccccggag tggaggcggc cgagcgcgcc ggggtgcccg ccttcctgga gacatccgcg
     9421 ccccgcaacc tccccttcta cgagcggctc ggcttcaccg tcaccgccga cgtcgaggtg
     9481 cccgaaggac cgcgcacctg gtgcatgacc cgcaagcccg gtgcctgaca cgtgctacga
     9541 gatttcgatt ccaccgccgc cttctatgaa aggttgggct tcggaatcgt tttccgggac
     9601 gccggctgga tgatcctcca gcgcggggat ctcatgctgg agttcttcgc ccaccccaac
     9661 ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat
     9721 aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat
     9781 catgtctgta taccgtcgac ctctagctag agcttggcgt aatcatggtc attaccaatg
     9841 cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg
     9901 actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagcgctgc
     9961 gatgataccg cgagaaccac gctcaccggc tccggattta tcagcaataa accagccagc
    10021 cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa
    10081 ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc
    10141 catcgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg
    10201 ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc
    10261 cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat
    10321 ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg
    10381 tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc
    10441 ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg
    10501 aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat
    10561 gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg
    10621 gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg
    10681 ttgaatactc atattcttcc tt
//
