LOCUS       sequence-304307-       10732 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of cell-surface protein extracellular domain 
            fused to COMP5AP-AviTag-9xHis. Protein is secreted from cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10732)
  AUTHORS   Wojtowicz WM, Vielmetter J, Fernandes RA, Siepe DH, Eastman CL, 
            Chisholm GB, Cox S, Klock H, Anderson PW, Rue SM, Miller JJ, Glaser 
            SM, Bragstad ML, Vance J, Lam AW, Lesley SA, Zinn K, Garcia KC
  TITLE     A Human IgSF Cell-Surface Interactome Reveals a Complex Network of 
            Protein-Protein Interactions.
  JOURNAL   Cell. 2020 Aug 20;182(4):1027-1043.e17. doi: 
            10.1016/j.cell.2020.07.025.
  PUBMED    32822567
REFERENCE   2  (bases 1 to 10732)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2020.07"; journalName: "Cell"; date: "2020-08-20- 
            20"; volume: "182"; issue: "4"; pages: "1027-1043.e17"
COMMENT     Sequence Label: pD649-HAsp-NFASC-COMP5AP-AviTag-9xHis
FEATURES             Location/Qualifiers
     source          1..10732
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     124..143
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     149..168
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     rep_origin      254..842
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     743..762
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     996..1013
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     1111..1128
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     1112..1128
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    1584..1600
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     enhancer        1767..2146
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2147..2350
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2301..2321
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     2347..2371
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        2581..2599
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     2581..2598
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     2612..2631
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     CDS             6337..6372
                     /codon_start=1
                     /product="epitope tag derived from human Protein C (Stearns
                     et al., 1988)"
                     /label=Protein C tag
                     /translation="EDQVDPRLIDGK"
     CDS             6382..6405
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     CDS             8065..8109
                     /codon_start=1
                     /product="peptide tag that allows for enzymatic 
                     biotinylation"
                     /label=AviTag(TM)
                     /translation="GLNDIFEAQKIEWHE"
     CDS             8110..8136
                     /codon_start=1
                     /product="9xHis affinity tag"
                     /label=9xHis
                     /translation="HHHHHHHHH"
     primer_bind     complement(8276..8293)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    8282..8393
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(8511..8531)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        8516..8845
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      8696..8831
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     8758..8777
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        8893..8940
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             8959..9558
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(8959..8978)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     9455..9475
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     polyA_signal    9688..9809
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9725..9744)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9779..9798
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     CDS             complement(join(9862..10653,10654..10722))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10485..10504
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(join(10723..10732,1..82))
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa
       61 tgtatttaga aaaataaaca aataggggtc agtgttacaa ccaattaacc aattctgaac
      121 attatcgcga gcccatttat acctgaatat ggctcataac accccttgct catgaccaaa
      181 atcccttaac gtgagttacg cgcgcgtcgt tccactgagc gtcagacccc gtagaaaaga
      241 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa
      301 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga
      361 aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt
      421 tagcccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt
      481 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat
      541 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct
      601 tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca
      661 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
      721 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
      781 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
      841 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
      901 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
      961 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg
     1021 aaggcgagag tagggaactg ccaggcatca aactaagcag aaggcccctg acggatggcc
     1081 tttttgcgtt tctacaaact ctttctgtgt tgtaaaacga cggccagtct taagctcggg
     1141 ccccctgggc ggttctgata acgagtaatc gttaatccgc aaataacgta aaaacccgct
     1201 tcggcgggtt tttttatggg gggagtttag ggaaagagca tttgtcagaa tatttaaggg
     1261 cgcctgtcac tttgcttgat atatgagaat tatttaacct tataaatgag aaaaaagcaa
     1321 cgcactttaa ataagatacg ttgctttttc gattgatgaa cacctataat taaactattc
     1381 atctattatt tatgattttt tgtatataca atatttctag tttgttaaag agaattaaga
     1441 aaataaatct cgaaaataat aaagggaaaa tcagtttttg atatcaaaat tatacatgtc
     1501 aacgataata caaaatataa tacaaactat aagatgttat cagtatttat tatcatttag
     1561 aataaatttt gtgtcgccct taattgtgag cggataacaa ttacgagctt catgcacagt
     1621 ggcgttgact caatattggc cattagccat attattcatt ggttatatag cataaatcaa
     1681 tattggctat tggccattgc atacgttgta tctatatcat aatatgtaca tttatattgg
     1741 ctcatgtcca atatgaccgc catgttggca ttgattattg actagttatt aatagtaatc
     1801 aattacgggg tcattagttc atagcccata tatggagttc cgcgttacat aacttacggt
     1861 aaatggcccg cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta
     1921 tgttcccata gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg
     1981 gtaaactgcc cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga
     2041 cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag tacatgacct tatgggactt
     2101 tcctacttgg cagtacatct acgtattagt catcgctatt accatggtga tgcggttttg
     2161 gcagtacatc aatgggcgtg gatagcggtt tgactcacgg ggatttccaa gtctccaccc
     2221 cattgacgtc aatgggagtt tgttttggca ccaaaatcaa cgggactttc caaaatgtcg
     2281 taacaactcc gccccattga cgcaaatggg cggtaggcgt gtacggtggg aggtctatat
     2341 aagcagagct cgtttagtga accgtcagat cgcctggaga cgccatccac gctgttttga
     2401 cctccataga agacaccggg accgatccag cctccgcggc cgggaacggt gcattggaac
     2461 gcggattccc cgtgccaaga gtgacgtaag taccgcctat agagtctata ggcccacccc
     2521 cttggcttcg ttagaacgcg gctacaatta atacataacc ttatgtatca tacacatacg
     2581 atttaggtga cactatagaa taacatccac tttgcctttc tctccacagg tgtccactcc
     2641 caggtccaac tgcacctcgg ttctatcgaa aacgcgcctg ctagcgccac catgaagacc
     2701 atcattgccc tgagctacat cttctgcctg gtgttcgctg cggccgccat tgagatccct
     2761 atggacccct ctatccagaa cgagctgacc cagcctccta ccatcacaaa gcagagcgcc
     2821 aaggaccaca tcgtggaccc cagagacaac atcctgatcg agtgcgaggc caagggcaac
     2881 cctgctccta gcttccactg gacccggaac agccggttct tcaatatcgc caaagatccc
     2941 cgggtgtcca tgagaaggcg ctctggcaca ctggtcatcg actttagaag cggcggcaga
     3001 cctgaggaat acgagggcga gtaccagtgc ttcgcccgga acaaatttgg cacagccctg
     3061 agcaaccgga tcagactcca ggtgtccaag tctcccctgt ggcctaaaga aaatctggac
     3121 cccgtggtgg tgcaagaggg tgctcctctg actctgcagt gcaatcctcc tcctggactg
     3181 ccctctcctg tgatcttttg gatgagcagc agcatggaac ccatcacaca ggacaagaga
     3241 gtgtcccagg gccacaacgg cgacctgtac ttcagcaacg tgatgctgca ggacatgcag
     3301 accgactaca gctgcaacgc cagattccac ttcacccaca ccatccagca gaagaacccc
     3361 ttcacactga aggtgctgac cacaagaggc gtggccgaga gaacccctag cttcatgtat
     3421 cctcagggca ccgccagcag ccagatggtg cttagaggaa tggacctgct gctggaatgt
     3481 atcgccagcg gcgtgccaac acctgatatc gcctggtaca agaaaggcgg cgatctgccc
     3541 agcgacaagg ccaagttcga gaacttcaac aaggccctgc ggatcaccaa cgtgtccgag
     3601 gaagatagcg gcgagtactt ctgcctggcc agcaacaaga tgggcagcat cagacacacc
     3661 atcagcgtca gagtgaaggc cgctccttac tggctggacg agcccaagaa tctgattctg
     3721 gcccctggcg aagatggcag actcgtgtgt agagccaacg gcaatcctaa gcctaccgtg
     3781 cagtggatgg tcaacggcga acctctgcag tctgcccctc ctaatcctaa tagagaggtg
     3841 gccggcgaca ccatcatctt cagggatacc cagatcagca gcagagccgt gtaccagtgt
     3901 aacaccagca acgagcacgg ctacctgctg gccaatgctt tcgtgtctgt gctggacgtg
     3961 ccacctagaa tgctgagccc cagaaaccag ctgatccgcg tgatcctgta caacagaacc
     4021 cggctggatt gcccattctt cggcagcccc attcctacac tgcggtggtt taagaacggc
     4081 cagggctcta acctggacgg cggcaattac cacgtgtacg agaacggcag cctggaaatc
     4141 aagatgatcc ggaaagagga ccagggcatc tacacatgcg tggccaccaa tatcctgggc
     4201 aaagccgaga atcaagtgcg gctggaagtg aaggacccca ccagaatcta cagaatgccc
     4261 gaggatcagg tggccagacg gggaacaaca gtgcagctgg aatgcagagt gaaacacgac
     4321 cccagcctga agctgaccgt gtcctggctg aaggacgacg agcctctgta catcggcaac
     4381 aggatgaaga aagaggatga cagcctgacc atcttcggag tggccgaaag agatcagggc
     4441 tcctatacct gtgtggccag caccgaactg gaccaggatc tggccaaagc ctatctgaca
     4501 gtgctggccg atcaggccac acctacaaat agactggccg ctctgcctaa gggcagaccc
     4561 gatagaccta gagatctgga actgaccgac ctggctgaga gatctgtgcg gcttacatgg
     4621 attcccggcg acgccaacaa cagccccatc acagattacg tggtgcagtt cgaagaggat
     4681 cagtttcagc ccggcgtgtg gcacgatcac agcaagtatc ctggctccgt gaacagcgcc
     4741 gtgctgagac tgagccctta cgtgaactac cagttcagag tgatcgccat caatgaagtg
     4801 ggcagctctc accctagcct gcctagcgag agatacagaa caagcggagc ccctcctgag
     4861 agcaaccctg gggatgtgaa aggcgagggc accagaaaga acaacatgga aatcacctgg
     4921 acacccatga acgccaccag cgcctttgga cccaacctgc ggtatattgt gaagtggcgg
     4981 cggagagaga cacgcgaggc ttggaacaat gtgaccgtgt ggggcagcag atacgtcgtg
     5041 ggacagacac ctgtgtacgt gccctacgag attagagtgc aggccgagaa cgatttcggc
     5101 aagggacctg agcctgagag cgtgatcgga tacagcggcg aggattatcc tagagccgct
     5161 cctaccgaag tgaaagtgcg cgtgatgaac agcacagcca tcagcctgca gtggaacaga
     5221 gtgtacagcg acacagtgca gggccagctg agagagtacc gggcctacta ttggagagag
     5281 agcagcctgc tgaagaacct gtgggtgtcc cagaagagac agcaggccag ctttcctggc
     5341 gatagactga gaggcgtcgt gtccagactg ttcccctact ccaactacaa gctggaaatg
     5401 gtggtcgtga acggcagagg cgacggccct agaagcgaga caaaagagtt taccacacct
     5461 gagggcgtgc ccagcgctcc tagaaggttt agagtgcggc agcccaacct ggaaaccatc
     5521 aatctggaat gggatcaccc tgagcacccc aacggcatca tgatcggcta caccctgaaa
     5581 tacgtggcct tcaacggcac caaagtgggc aagcagatcg tggaaaactt cagccccaac
     5641 cagaccaagt tcaccgtgca gagaaccgat ccagtgtcca gataccggtt caccctgtcc
     5701 gccagaacac aagttggatc tggcgaggcc gtgaccgagg aatctcctgc tcctccaaat
     5761 gaggccactc caacagctgc tccaccaaca ctgccaccta caacagttgg agccacaggc
     5821 gccgtgtcta gcacagatgc tacagccatt gccgccacca cagaggccac aaccgtgcct
     5881 attattccca cagtggcccc taccacaatc gccacaacaa ccaccgtggc taccaccaca
     5941 actaccacag ccgctgccac aaccacaaca gagagccctc caacaacaac ctccggcacc
     6001 aagatccacg agagcgcccc tgatgagcag tccatttgga atgtgactgt gctgcctaac
     6061 agcaagtggg ccaacatcac atggaagcac aacttcggcc ctggcaccga cttcgtggtc
     6121 gagtacatcg acagcaacca caccaaaaag accgtgcctg tgaaggccca ggctcagcct
     6181 attcagctga ccgatctgta ccccggcatg acctacacac tgcgggtgta cagcagagac
     6241 aatgagggca tcagctccac cgtgatcacc ttcatgacca gcaccgccta caccaacaac
     6301 caggccgata ttgccacaca aggctggggc gcgccggaag atcaagttga tccaagattg
     6361 attgatggca agggcggatc tctggaggtg ctgttccagg gaccaggcgg aagcggcaat
     6421 cccggcggcg atctggcccc ccagatgctg cgcgagctgc aggagaccaa tgccgccctg
     6481 caggacgtgc gcgagctgct gcgccaccgc gtgaaggaga tcaccttcct gaagaacacc
     6541 gtgatggagt gcgacgcctg cggtatgcag ccctctagag gctccggctc catcatccca
     6601 gttgaggagg agaacccgga cttctggaac cgcgaggcag ccgaggccct gggtgccgcc
     6661 aagaagctgc agcctgcaca gacagccgcc aagaacctca tcatcttcct gggcgatggg
     6721 atgggggtgt ctacggtgac agctgccagg atcctaaaag ggcagaagaa ggacaaactg
     6781 gggcctgaga tacccctggc catggaccgc ttcccatatg tggctctgtc caagacatac
     6841 aatgtagaca aacatgtgcc agacagtgga gccacagcca cggcctacct gtgcggggtc
     6901 aagggcaact tccagaccat tggcttgagt gcagccgccc gctttaacca gtgcaacacg
     6961 acacgcggca acgaggtcat ctccgtgatg aatcgggcca agaaagcagg gaagtcagtg
     7021 ggagtggtaa ccaccacacg agtgcagcac gcctcgccag ccggcaccta cgcccacacg
     7081 gtgaaccgca actggtactc ggacgccgac gtgcctgcct cggcccgcca ggaggggtgc
     7141 caggacatcg ctacgcagct catctccaac atggacattg acgtgatcct aggtggaggc
     7201 cgaaagtaca tgtttcgcat gggaacccca gaccctgagt acccagatga ctacagccaa
     7261 ggtgggacca ggctggacgg gaagaatctg gtgcaggaat ggctggcgaa gcgccagggt
     7321 gcccggtatg tgtggaaccg cactgagctc atgcaggctt ccctggaccc gtctgtgacc
     7381 catctcatgg gtctctttga gcctggagac atgaaatacg agatccaccg agactccaca
     7441 ctggacccct ccctgatgga gatgacagag gctgccctgc gcctgctgag caggaacccc
     7501 cgcggcttct tcctcttcgt ggagggtggt cgcatcgacc atggtcatca tgaaagcagg
     7561 gcttaccggg cactgactga gacgatcatg ttcgacgacg ccattgagag ggcgggccag
     7621 ctcaccagcg aggaggacac gctgagcctc gtcactgccg accactccca cgtcttctcc
     7681 ttcggaggct accccctgcg agggagctcc atcttcgggc tggcccctgg caaggcccgg
     7741 gacaggaagg cctacacggt cctcctatac ggaaacggtc caggctatgt gctcaaggac
     7801 ggcgcccggc cggatgttac cgagagcgag agcgggagcc ccgagtatcg gcagcagtca
     7861 gcagtgcccc tggacgaaga gacccacgca ggcgaggacg tggcggtgtt cgcgcgcggc
     7921 ccgcaggcgc acctggttca cggcgtgcag gagcagacct tcatagcgca cgtcatggcc
     7981 ttcgccgcct gcctggagcc ctacaccgcc tgcgacctgg cgccccccgc cggcaccacc
     8041 gacgccgcgc acccgggtgg cggaggactg aacgacatct tcgaggctca gaaaatcgaa
     8101 tggcacgaac atcaccatca ccatcaccat caccattaag gccggcctgt agtcactggc
     8161 acaacgcgtt acccggtaag ccaatcgggt atacacggtc gtcatactgc agacagggtt
     8221 cttctacttt gcaagatagt cttgagtagt aaaataaata gatagagaaa atcagcctcg
     8281 actgtgcctt ctagttgcca gccatctgtt gtttgcccct cccccgtgcc ttccttgacc
     8341 ctggaaggtg ccactcccac tgtcctttcc taataaaatg aggaaattgc atcacaacac
     8401 tcaaccctat ctcggtctat tcttttgatt tataagggat tttgccgatt tcggcctatt
     8461 ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttaattctgt ggaatgtgtg
     8521 tcagttaggg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca
     8581 tctcaattag tcagcaacca ggtgtggaaa gtccccaggc tccccagcag gcagaagtat
     8641 gcaaagcatg catctcaatt agtcagcaac catagtcccg cccctaactc cgcccatccc
     8701 gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat
     8761 ttatgcagag gccgaggccg cctctgcctc tgagctattc cagaagtagt gaggaggctt
     8821 ttttggaggc ctaggctttt gcaaaaagct cccgggagct tgtatatcca ttttcggatc
     8881 tgatcagcac gtgttgacaa ttaatcatcg gcatagtata tcggcatagt ataatacgac
     8941 aaggtgagga actaaaccat gaccgagtac aagcccacgg tgcgcctcgc cacccgcgac
     9001 gacgtcccca gggccgtacg caccctcgcc gccgcgttcg ccgactaccc cgccacgcgc
     9061 cacaccgtcg atccggaccg ccacatcgag cgggtcaccg agctgcaaga actcttcctc
     9121 acgcgcgtcg ggctcgacat cggcaaggtg tgggtcgcgg acgacggcgc cgcggtggcg
     9181 gtctggacca cgccggagag cgtcgaagcg ggggcggtgt tcgccgagat cggcccgcgc
     9241 atggccgagt tgagcggttc ccggctggcc gcgcagcaac agatggaagg cctcctggcg
     9301 ccgcaccggc ccaaggagcc cgcgtggttc ctggccaccg tcggcgtctc gcccgaccac
     9361 cagggcaagg gtctgggcag cgccgtcgtg ctccccggag tggaggcggc cgagcgcgcc
     9421 ggggtgcccg ccttcctgga gacatccgcg ccccgcaacc tccccttcta cgagcggctc
     9481 ggcttcaccg tcaccgccga cgtcgaggtg cccgaaggac cgcgcacctg gtgcatgacc
     9541 cgcaagcccg gtgcctgaca cgtgctacga gatttcgatt ccaccgccgc cttctatgaa
     9601 aggttgggct tcggaatcgt tttccgggac gccggctgga tgatcctcca gcgcggggat
     9661 ctcatgctgg agttcttcgc ccaccccaac ttgtttattg cagcttataa tggttacaaa
     9721 taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt
     9781 ggtttgtcca aactcatcaa tgtatcttat catgtctgta taccgtcgac ctctagctag
     9841 agcttggcgt aatcatggtc attaccaatg cttaatcagt gaggcaccta tctcagcgat
     9901 ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg
     9961 ggagggctta ccatctggcc ccagcgctgc gatgataccg cgagaaccac gctcaccggc
    10021 tccggattta tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc
    10081 aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc
    10141 gccagttaat agtttgcgca acgttgttgc catcgctaca ggcatcgtgg tgtcacgctc
    10201 gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc
    10261 ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa
    10321 gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat
    10381 gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata
    10441 gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca
    10501 tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag
    10561 gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc
    10621 agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc
    10681 aaaaaaggga ataagggcga cacggaaatg ttgaatactc atattcttcc tt
//
