LOCUS       sequence-304309-       10855 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of cell-surface protein extracellular domain 
            fused to COMP5AP-AviTag-9xHis. Protein is secreted from cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10855)
  AUTHORS   Wojtowicz WM, Vielmetter J, Fernandes RA, Siepe DH, Eastman CL, 
            Chisholm GB, Cox S, Klock H, Anderson PW, Rue SM, Miller JJ, Glaser 
            SM, Bragstad ML, Vance J, Lam AW, Lesley SA, Zinn K, Garcia KC
  TITLE     A Human IgSF Cell-Surface Interactome Reveals a Complex Network of 
            Protein-Protein Interactions.
  JOURNAL   Cell. 2020 Aug 20;182(4):1027-1043.e17. doi: 
            10.1016/j.cell.2020.07.025.
  PUBMED    32822567
REFERENCE   2  (bases 1 to 10855)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2020.07"; journalName: "Cell"; date: "2020-08-20- 
            20"; volume: "182"; issue: "4"; pages: "1027-1043.e17"
COMMENT     Sequence Label: pD649-HAsp-PTPRF-COMP5AP-AviTag-9xHis
FEATURES             Location/Qualifiers
     source          1..10855
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     124..143
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     149..168
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     rep_origin      254..842
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     743..762
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     996..1013
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     1111..1128
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     1112..1128
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    1584..1600
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     enhancer        1767..2146
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2147..2350
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2301..2321
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     2347..2371
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        2581..2599
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     2581..2598
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     2612..2631
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     CDS             6460..6495
                     /codon_start=1
                     /product="epitope tag derived from human Protein C (Stearns
                     et al., 1988)"
                     /label=Protein C tag
                     /translation="EDQVDPRLIDGK"
     CDS             6505..6528
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     CDS             8188..8232
                     /codon_start=1
                     /product="peptide tag that allows for enzymatic 
                     biotinylation"
                     /label=AviTag(TM)
                     /translation="GLNDIFEAQKIEWHE"
     CDS             8233..8259
                     /codon_start=1
                     /product="9xHis affinity tag"
                     /label=9xHis
                     /translation="HHHHHHHHH"
     primer_bind     complement(8399..8416)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    8405..8516
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(8634..8654)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        8639..8968
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      8819..8954
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     8881..8900
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        9016..9063
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             9082..9681
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(9082..9101)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     9578..9598
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     polyA_signal    9811..9932
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9848..9867)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9902..9921
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     CDS             complement(join(9985..10776,10777..10845))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10608..10627
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(join(10846..10855,1..82))
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa
       61 tgtatttaga aaaataaaca aataggggtc agtgttacaa ccaattaacc aattctgaac
      121 attatcgcga gcccatttat acctgaatat ggctcataac accccttgct catgaccaaa
      181 atcccttaac gtgagttacg cgcgcgtcgt tccactgagc gtcagacccc gtagaaaaga
      241 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa
      301 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga
      361 aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt
      421 tagcccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt
      481 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat
      541 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct
      601 tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca
      661 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
      721 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
      781 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
      841 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
      901 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
      961 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg
     1021 aaggcgagag tagggaactg ccaggcatca aactaagcag aaggcccctg acggatggcc
     1081 tttttgcgtt tctacaaact ctttctgtgt tgtaaaacga cggccagtct taagctcggg
     1141 ccccctgggc ggttctgata acgagtaatc gttaatccgc aaataacgta aaaacccgct
     1201 tcggcgggtt tttttatggg gggagtttag ggaaagagca tttgtcagaa tatttaaggg
     1261 cgcctgtcac tttgcttgat atatgagaat tatttaacct tataaatgag aaaaaagcaa
     1321 cgcactttaa ataagatacg ttgctttttc gattgatgaa cacctataat taaactattc
     1381 atctattatt tatgattttt tgtatataca atatttctag tttgttaaag agaattaaga
     1441 aaataaatct cgaaaataat aaagggaaaa tcagtttttg atatcaaaat tatacatgtc
     1501 aacgataata caaaatataa tacaaactat aagatgttat cagtatttat tatcatttag
     1561 aataaatttt gtgtcgccct taattgtgag cggataacaa ttacgagctt catgcacagt
     1621 ggcgttgact caatattggc cattagccat attattcatt ggttatatag cataaatcaa
     1681 tattggctat tggccattgc atacgttgta tctatatcat aatatgtaca tttatattgg
     1741 ctcatgtcca atatgaccgc catgttggca ttgattattg actagttatt aatagtaatc
     1801 aattacgggg tcattagttc atagcccata tatggagttc cgcgttacat aacttacggt
     1861 aaatggcccg cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta
     1921 tgttcccata gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg
     1981 gtaaactgcc cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga
     2041 cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag tacatgacct tatgggactt
     2101 tcctacttgg cagtacatct acgtattagt catcgctatt accatggtga tgcggttttg
     2161 gcagtacatc aatgggcgtg gatagcggtt tgactcacgg ggatttccaa gtctccaccc
     2221 cattgacgtc aatgggagtt tgttttggca ccaaaatcaa cgggactttc caaaatgtcg
     2281 taacaactcc gccccattga cgcaaatggg cggtaggcgt gtacggtggg aggtctatat
     2341 aagcagagct cgtttagtga accgtcagat cgcctggaga cgccatccac gctgttttga
     2401 cctccataga agacaccggg accgatccag cctccgcggc cgggaacggt gcattggaac
     2461 gcggattccc cgtgccaaga gtgacgtaag taccgcctat agagtctata ggcccacccc
     2521 cttggcttcg ttagaacgcg gctacaatta atacataacc ttatgtatca tacacatacg
     2581 atttaggtga cactatagaa taacatccac tttgcctttc tctccacagg tgtccactcc
     2641 caggtccaac tgcacctcgg ttctatcgaa aacgcgcctg ctagcgccac catgaagacc
     2701 atcattgccc tgagctacat cttctgcctg gtgttcgctg cggccgccga tagcaagcct
     2761 gtgtttatca aggtgcccga ggaccagact ggactgtctg gcggagttgc cagctttgtg
     2821 tgtcaggcca caggcgagcc taagcctaga atcacctgga tgaagaaagg caagaaggtg
     2881 tccagccagc gcttcgaagt gatcgagttt gatgatggcg ccggaagcgt gctgagaatc
     2941 cagcctctga gagtgcagag agatgaggcc atctacgagt gcaccgccac aaatagcctg
     3001 ggcgagatca ataccagcgc caagctgagc gtgctggaag aggaacagct gcctcctggc
     3061 ttcccctcta tcgatatggg acctcagctg aaggtggtgg aaaaggccag aaccgccact
     3121 atgctttgtg ccgctggcgg caatcctgat cctgagatca gctggttcaa ggacttcctg
     3181 cctgtggacc ctgccaccag caatggcaga atcaagcagc tgagatctgg cgccctgcag
     3241 atcgagagca gcgaggaaag cgatcagggc aaatacgagt gcgtggccac caatagcgcc
     3301 ggcacaagat attctgcccc tgccaacctg tacgtcagag tgcgaagagt ggcccctaga
     3361 ttcagcatcc ctcctagcag ccaagaagtg atgcctggcg gctccgtgaa tctgacatgt
     3421 gtggctgttg gcgcccctat gccttacgtg aaatggatga tgggagccga ggaactgacc
     3481 aaagaggacg agatgcccgt gggcagaaat gtgctggaac tgagcaatgt cgtgcggagc
     3541 gccaattaca cctgtgtggc catcagcagc ctgggcatga ttgaggccac agctcaagtg
     3601 acagtgaagg ccctgcctaa gccacctatc gacctggtgg ttaccgagac aaccgccacc
     3661 tctgtgaccc tgacatggga tagcggcaat agcgagccag tgacctacta cggcatccag
     3721 tatagagccg ccggaacaga gggccctttt caagaagttg acggcgtcgc caccaccaga
     3781 tatagcattg gcggactgag ccccttcagc gagtacgcct ttagagtgct ggccgtgaac
     3841 tctatcggca gaggacctcc atctgaggcc gttagagcta gaacaggcga acaggcccct
     3901 tctagccctc ctagaagagt gcaggctaga atgctgagcg ccagcacaat gctggtgcag
     3961 tgggaacctc ctgaggaacc caatggcctc gtgcggggct acagagtgta ctacacaccc
     4021 gatagcagac ggcctcctaa cgcctggcac aagcacaata ctgatgccgg cctgctgaca
     4081 accgtgggat ctctgctgcc tggcatcacc tatagcctgc gcgtgctggc ttttaccgcc
     4141 gttggagatg gaccaccttc tccaaccatc caagtgaaaa cccagcaggg cgttccagct
     4201 cagcctgccg attttcaggc cgaggtggaa agcgacaccc gcattcaact tagctggctg
     4261 ctgcctccac aagagcggat cattatgtac gagctggtgt actgggccgc tgaggatgag
     4321 gatcagcagc acaaagtgac cttcgatccc accagcagct acaccctgga agatctgaag
     4381 cccgacacac tgtacagatt ccagctggcc gctagaagcg atatgggcgt gggagtgttt
     4441 acccctacca tcgaagccag aacagcccag agcacaccta gcgctcctcc acagaaagtg
     4501 atgtgcgtgt ccatgggcag caccacagtc agagtgtctt gggtgccacc tcctgccgac
     4561 agcagaaatg gcgtgatcac acagtacagc gtggcctatg aagccgtgga tggcgaggat
     4621 agaggcagac acgtggtgga tggaatcagc agagagcaca gctcttggga tctcgtgggc
     4681 ctcgagaagt ggaccgagta tagagtgtgg gtccgagcac acacagatgt cggacctgga
     4741 cctgaaagca gccctgtgct cgtgcggaca gatgaggatg ttcctagcgg acctcctcgg
     4801 aaggtggaag tggaacctct gaatagcacc gccgtgcacg tgtactggaa gctgcctgtg
     4861 ccttctaagc agcacggcca gatcagaggc taccaagtga catacgtgcg gctggaaaat
     4921 ggcgagccaa gaggcctgcc tatcatccag gatgtgatgc tggctgaagc ccagtggcgg
     4981 cctgaggaat ctgaggacta cgaaaccacc atcagcgggc tgacccctga gacaacatac
     5041 tctgtgacag tggccgccta caccacaaaa ggcgacggcg ctagatccaa gcctaagatc
     5101 gtgaccacaa caggcgctgt gcctggcaga cctaccatga tgatcagcac caccgccatg
     5161 aacacagccc tgctgcagtg gcaccctcct aaagaactgc ctggcgagct gctgggctat
     5221 agactgcagt actgtagagc cgacgaggcc agacctaaca ccatcgactt cggcaaggac
     5281 gaccagcact ttacagtgac cggcctgcac aagggcacca cctacatttt tagactggcc
     5341 gccaagaaca gagccggcct gggagaagag ttcgagaaag agatcagaac cccagaggat
     5401 ctgcccagcg gcttccctca gaatctgcac gtgacaggcc tgaccacctc taccacagaa
     5461 cttgcttggg accctccagt gctggcagag agaaatggcc ggatcatctc ctacaccgtg
     5521 gtgttcagag acatcaacag ccagcaagag ctgcagaaca tcaccaccga caccagattc
     5581 accctgaccg gactgaagcc tgataccacc tacgacatca aagtccgcgc ctggaccagc
     5641 aaaggctctg gacctctgag ccctagcatc cagagcagaa ccatgccagt ggaacaggtg
     5701 ttcgccaaga atttcagagt ggccgctgcc atgaagacca gcgtgctgtt gtcttgggaa
     5761 gtgcccgaca gctacaagag cgccgtgcca ttcaagatcc tgtacaacgg ccagagcgtt
     5821 gaggtggacg gccacagcat gagaaagctg atcgccgacc tgcagcctaa caccgagtac
     5881 agcttcgtgc tgatgaacag aggaagctct gccggcggac tgcagcacct ggtgtctatc
     5941 agaacagctc ccgacctgct gccacacaaa cctctgcctg ccagcgccta tatcgaggac
     6001 ggcagattcg atctgagcat gccacacgtg caggaccctt ctctcgtgcg gtggttttac
     6061 atcgtggtgg tgcctatcga cagagtcggc ggatctatgc tgaccccaag atggtccaca
     6121 ccagaggaac tggaactgga cgaactgctg gaagccatcg agcaaggcgg cgaggaacaa
     6181 cgcagaagaa gaaggcaggc cgagcgcctg aaaccttatg tggctgctca actggacgtg
     6241 ctgcccgaga cattcacact gggcgacaag aagaactacc ggggcttcta caaccggcca
     6301 ctgagccctg atctgagcta ccagtgtttc gtgctggcct ctctgaaaga acccatggac
     6361 cagaagagat acgccagctc tccctacagc gacgagatcg tggtgcaagt gactcccgct
     6421 cagcagcaag aggaacccga aatgctttgg ggcgcgccgg aagatcaagt tgatccaaga
     6481 ttgattgatg gcaagggcgg atctctggag gtgctgttcc agggaccagg cggaagcggc
     6541 aatcccggcg gcgatctggc cccccagatg ctgcgcgagc tgcaggagac caatgccgcc
     6601 ctgcaggacg tgcgcgagct gctgcgccac cgcgtgaagg agatcacctt cctgaagaac
     6661 accgtgatgg agtgcgacgc ctgcggtatg cagccctcta gaggctccgg ctccatcatc
     6721 ccagttgagg aggagaaccc ggacttctgg aaccgcgagg cagccgaggc cctgggtgcc
     6781 gccaagaagc tgcagcctgc acagacagcc gccaagaacc tcatcatctt cctgggcgat
     6841 gggatggggg tgtctacggt gacagctgcc aggatcctaa aagggcagaa gaaggacaaa
     6901 ctggggcctg agatacccct ggccatggac cgcttcccat atgtggctct gtccaagaca
     6961 tacaatgtag acaaacatgt gccagacagt ggagccacag ccacggccta cctgtgcggg
     7021 gtcaagggca acttccagac cattggcttg agtgcagccg cccgctttaa ccagtgcaac
     7081 acgacacgcg gcaacgaggt catctccgtg atgaatcggg ccaagaaagc agggaagtca
     7141 gtgggagtgg taaccaccac acgagtgcag cacgcctcgc cagccggcac ctacgcccac
     7201 acggtgaacc gcaactggta ctcggacgcc gacgtgcctg cctcggcccg ccaggagggg
     7261 tgccaggaca tcgctacgca gctcatctcc aacatggaca ttgacgtgat cctaggtgga
     7321 ggccgaaagt acatgtttcg catgggaacc ccagaccctg agtacccaga tgactacagc
     7381 caaggtggga ccaggctgga cgggaagaat ctggtgcagg aatggctggc gaagcgccag
     7441 ggtgcccggt atgtgtggaa ccgcactgag ctcatgcagg cttccctgga cccgtctgtg
     7501 acccatctca tgggtctctt tgagcctgga gacatgaaat acgagatcca ccgagactcc
     7561 acactggacc cctccctgat ggagatgaca gaggctgccc tgcgcctgct gagcaggaac
     7621 ccccgcggct tcttcctctt cgtggagggt ggtcgcatcg accatggtca tcatgaaagc
     7681 agggcttacc gggcactgac tgagacgatc atgttcgacg acgccattga gagggcgggc
     7741 cagctcacca gcgaggagga cacgctgagc ctcgtcactg ccgaccactc ccacgtcttc
     7801 tccttcggag gctaccccct gcgagggagc tccatcttcg ggctggcccc tggcaaggcc
     7861 cgggacagga aggcctacac ggtcctccta tacggaaacg gtccaggcta tgtgctcaag
     7921 gacggcgccc ggccggatgt taccgagagc gagagcggga gccccgagta tcggcagcag
     7981 tcagcagtgc ccctggacga agagacccac gcaggcgagg acgtggcggt gttcgcgcgc
     8041 ggcccgcagg cgcacctggt tcacggcgtg caggagcaga ccttcatagc gcacgtcatg
     8101 gccttcgccg cctgcctgga gccctacacc gcctgcgacc tggcgccccc cgccggcacc
     8161 accgacgccg cgcacccggg tggcggagga ctgaacgaca tcttcgaggc tcagaaaatc
     8221 gaatggcacg aacatcacca tcaccatcac catcaccatt aaggccggcc tgtagtcact
     8281 ggcacaacgc gttacccggt aagccaatcg ggtatacacg gtcgtcatac tgcagacagg
     8341 gttcttctac tttgcaagat agtcttgagt agtaaaataa atagatagag aaaatcagcc
     8401 tcgactgtgc cttctagttg ccagccatct gttgtttgcc cctcccccgt gccttccttg
     8461 accctggaag gtgccactcc cactgtcctt tcctaataaa atgaggaaat tgcatcacaa
     8521 cactcaaccc tatctcggtc tattcttttg atttataagg gattttgccg atttcggcct
     8581 attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattaattc tgtggaatgt
     8641 gtgtcagtta gggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat
     8701 gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag caggcagaag
     8761 tatgcaaagc atgcatctca attagtcagc aaccatagtc ccgcccctaa ctccgcccat
     8821 cccgccccta actccgccca gttccgccca ttctccgccc catggctgac taattttttt
     8881 tatttatgca gaggccgagg ccgcctctgc ctctgagcta ttccagaagt agtgaggagg
     8941 cttttttgga ggcctaggct tttgcaaaaa gctcccggga gcttgtatat ccattttcgg
     9001 atctgatcag cacgtgttga caattaatca tcggcatagt atatcggcat agtataatac
     9061 gacaaggtga ggaactaaac catgaccgag tacaagccca cggtgcgcct cgccacccgc
     9121 gacgacgtcc ccagggccgt acgcaccctc gccgccgcgt tcgccgacta ccccgccacg
     9181 cgccacaccg tcgatccgga ccgccacatc gagcgggtca ccgagctgca agaactcttc
     9241 ctcacgcgcg tcgggctcga catcggcaag gtgtgggtcg cggacgacgg cgccgcggtg
     9301 gcggtctgga ccacgccgga gagcgtcgaa gcgggggcgg tgttcgccga gatcggcccg
     9361 cgcatggccg agttgagcgg ttcccggctg gccgcgcagc aacagatgga aggcctcctg
     9421 gcgccgcacc ggcccaagga gcccgcgtgg ttcctggcca ccgtcggcgt ctcgcccgac
     9481 caccagggca agggtctggg cagcgccgtc gtgctccccg gagtggaggc ggccgagcgc
     9541 gccggggtgc ccgccttcct ggagacatcc gcgccccgca acctcccctt ctacgagcgg
     9601 ctcggcttca ccgtcaccgc cgacgtcgag gtgcccgaag gaccgcgcac ctggtgcatg
     9661 acccgcaagc ccggtgcctg acacgtgcta cgagatttcg attccaccgc cgccttctat
     9721 gaaaggttgg gcttcggaat cgttttccgg gacgccggct ggatgatcct ccagcgcggg
     9781 gatctcatgc tggagttctt cgcccacccc aacttgttta ttgcagctta taatggttac
     9841 aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt
     9901 tgtggtttgt ccaaactcat caatgtatct tatcatgtct gtataccgtc gacctctagc
     9961 tagagcttgg cgtaatcatg gtcattacca atgcttaatc agtgaggcac ctatctcagc
    10021 gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat
    10081 acgggagggc ttaccatctg gccccagcgc tgcgatgata ccgcgagaac cacgctcacc
    10141 ggctccggat ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc
    10201 tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag
    10261 ttcgccagtt aatagtttgc gcaacgttgt tgccatcgct acaggcatcg tggtgtcacg
    10321 ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg
    10381 atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag
    10441 taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt
    10501 catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga
    10561 atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc
    10621 acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc
    10681 aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc
    10741 ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc
    10801 cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatattct tcctt
//
