LOCUS       sequence-304319-       11473 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of cell-surface protein partial extracellular 
            domain fused to COMP5AP-AviTag-9xHis. Protein is secreted from 
            cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11473)
  AUTHORS   Wojtowicz WM, Vielmetter J, Fernandes RA, Siepe DH, Eastman CL, 
            Chisholm GB, Cox S, Klock H, Anderson PW, Rue SM, Miller JJ, Glaser 
            SM, Bragstad ML, Vance J, Lam AW, Lesley SA, Zinn K, Garcia KC
  TITLE     A Human IgSF Cell-Surface Interactome Reveals a Complex Network of 
            Protein-Protein Interactions.
  JOURNAL   Cell. 2020 Aug 20;182(4):1027-1043.e17. doi: 
            10.1016/j.cell.2020.07.025.
  PUBMED    32822567
REFERENCE   2  (bases 1 to 11473)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2020.07"; journalName: "Cell"; date: "2020-08-20- 
            20"; volume: "182"; issue: "4"; pages: "1027-1043.e17"
COMMENT     Sequence Label: pD649-HAsp-PXDNL(Trunc1)-COMP5AP-AviTag-9xHis
FEATURES             Location/Qualifiers
     source          1..11473
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     124..143
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     149..168
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     rep_origin      254..842
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     743..762
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     996..1013
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     1111..1128
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     1112..1128
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    1584..1600
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     enhancer        1767..2146
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2147..2350
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2301..2321
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     2347..2371
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        2581..2599
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     2581..2598
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     2612..2631
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     CDS             7078..7113
                     /codon_start=1
                     /product="epitope tag derived from human Protein C (Stearns
                     et al., 1988)"
                     /label=Protein C tag
                     /translation="EDQVDPRLIDGK"
     CDS             7123..7146
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     CDS             8806..8850
                     /codon_start=1
                     /product="peptide tag that allows for enzymatic 
                     biotinylation"
                     /label=AviTag(TM)
                     /translation="GLNDIFEAQKIEWHE"
     CDS             8851..8877
                     /codon_start=1
                     /product="9xHis affinity tag"
                     /label=9xHis
                     /translation="HHHHHHHHH"
     primer_bind     complement(9017..9034)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    9023..9134
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(9252..9272)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        9257..9586
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      9437..9572
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     9499..9518
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        9634..9681
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             9700..10299
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(9700..9719)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     10196..10216
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     polyA_signal    10429..10550
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10466..10485)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10520..10539
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     CDS             complement(join(10603..11394,11395..11463))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11226..11245
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(join(11464..11473,1..82))
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa
       61 tgtatttaga aaaataaaca aataggggtc agtgttacaa ccaattaacc aattctgaac
      121 attatcgcga gcccatttat acctgaatat ggctcataac accccttgct catgaccaaa
      181 atcccttaac gtgagttacg cgcgcgtcgt tccactgagc gtcagacccc gtagaaaaga
      241 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa
      301 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga
      361 aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt
      421 tagcccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt
      481 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat
      541 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct
      601 tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca
      661 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
      721 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
      781 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
      841 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
      901 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
      961 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg
     1021 aaggcgagag tagggaactg ccaggcatca aactaagcag aaggcccctg acggatggcc
     1081 tttttgcgtt tctacaaact ctttctgtgt tgtaaaacga cggccagtct taagctcggg
     1141 ccccctgggc ggttctgata acgagtaatc gttaatccgc aaataacgta aaaacccgct
     1201 tcggcgggtt tttttatggg gggagtttag ggaaagagca tttgtcagaa tatttaaggg
     1261 cgcctgtcac tttgcttgat atatgagaat tatttaacct tataaatgag aaaaaagcaa
     1321 cgcactttaa ataagatacg ttgctttttc gattgatgaa cacctataat taaactattc
     1381 atctattatt tatgattttt tgtatataca atatttctag tttgttaaag agaattaaga
     1441 aaataaatct cgaaaataat aaagggaaaa tcagtttttg atatcaaaat tatacatgtc
     1501 aacgataata caaaatataa tacaaactat aagatgttat cagtatttat tatcatttag
     1561 aataaatttt gtgtcgccct taattgtgag cggataacaa ttacgagctt catgcacagt
     1621 ggcgttgact caatattggc cattagccat attattcatt ggttatatag cataaatcaa
     1681 tattggctat tggccattgc atacgttgta tctatatcat aatatgtaca tttatattgg
     1741 ctcatgtcca atatgaccgc catgttggca ttgattattg actagttatt aatagtaatc
     1801 aattacgggg tcattagttc atagcccata tatggagttc cgcgttacat aacttacggt
     1861 aaatggcccg cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta
     1921 tgttcccata gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg
     1981 gtaaactgcc cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga
     2041 cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag tacatgacct tatgggactt
     2101 tcctacttgg cagtacatct acgtattagt catcgctatt accatggtga tgcggttttg
     2161 gcagtacatc aatgggcgtg gatagcggtt tgactcacgg ggatttccaa gtctccaccc
     2221 cattgacgtc aatgggagtt tgttttggca ccaaaatcaa cgggactttc caaaatgtcg
     2281 taacaactcc gccccattga cgcaaatggg cggtaggcgt gtacggtggg aggtctatat
     2341 aagcagagct cgtttagtga accgtcagat cgcctggaga cgccatccac gctgttttga
     2401 cctccataga agacaccggg accgatccag cctccgcggc cgggaacggt gcattggaac
     2461 gcggattccc cgtgccaaga gtgacgtaag taccgcctat agagtctata ggcccacccc
     2521 cttggcttcg ttagaacgcg gctacaatta atacataacc ttatgtatca tacacatacg
     2581 atttaggtga cactatagaa taacatccac tttgcctttc tctccacagg tgtccactcc
     2641 caggtccaac tgcacctcgg ttctatcgaa aacgcgcctg ctagcgccac catgaagacc
     2701 atcattgccc tgagctacat cttctgcctg gtgttcgctg cggccgcctg tcctagcaga
     2761 tgcctgtgtt ttaagagcac cgtgcgctgc atgcacctga tgctggatca cattcctcag
     2821 gtgccacagc agaccaccgt gctggacctg cggttcaaca gaatcagaga gatccccggc
     2881 agcgccttca agaagctgaa gaacctgaac accctgctgc tgaacaacaa ccacatccgg
     2941 aagatcagcc ggaacgcctt tgaaggcctg gaaaacctgc tgtacctgta cctctacaag
     3001 aacgagattc acgccctgga caagcagacc ttcaagggcc tgatttccct ggaacacctg
     3061 tacatccact tcaaccagct ggaaatgctg cagcccgaga cattcggcga cctgctgaga
     3121 ctggaacggc tgttcctgca caacaacaag ctgagcaaga tcccagccgg cagcttcagc
     3181 aacctggaca gcctgaaaag actgcggctg gacagcaatg ccctcgtgtg cgattgtgat
     3241 ctgatgtggc tgggagagct gctgcaggga tttgcccagc atggacatac acaggccgct
     3301 gccacctgtg aataccctag aaggctgcat ggcagagccg tggcctctgt gaccgtggaa
     3361 gagttcaact gtcagagccc cagaatcacc ttcgagcccc aagatgtgga agtgcccagc
     3421 ggcaacaccg tgtactttac atgcagagcc gagggcaacc ccaagcctga gatcatctgg
     3481 attcacaaca atcacagcct ggacctggaa gatgacaccc ggctgaacgt gttcgacgac
     3541 ggcaccctga tgatccggaa caccagagaa agcgatcagg gcgtgtacca gtgcatggcc
     3601 agaaattctg ccggcgaggc caagacacag agcgccatgc tgagatacag cagcctgcct
     3661 gccaagccta gcttcgtgat tcagcctcag gacaccgagg tgctgatcgg cacaagcacc
     3721 acactggaat gcatggctac cggccatcct catcctctga tcacctggac cagagacaac
     3781 ggcctggaac tggatggaag cagacacgtg gcaaccagct ctggcctgta cctgcagaac
     3841 atcacccaac gcgaccacgg cagattcacc tgtcacgcca acaatagcca cggcacagtg
     3901 caggctgccg ccaatattat cgttcaggcc cctcctcagt tcaccgtgac acctaaagat
     3961 caggtggtgc tggaagaaca cgccgtggaa tggctgtgcg aggccgatgg aaatcctcct
     4021 cctgtgatcg tgtggaccaa gacaggtgga cagctgccag tggaaggaca gcacacagtg
     4081 ctgtctagcg gcacactgag aatcgacaga gccgctcagc acgaccaggg ccagtatgaa
     4141 tgtcaagccg tgtccagcct gggcgtgaag aaagtgtctg tgcagctgac cgtgaagccc
     4201 aaggctctgg ctgtgtttac ccagctgcct caggatacaa gcgtggaagt gggcaagaac
     4261 atcaacatca gctgccacgc tcagggcgag cctcagccta tcatcacatg gaacaaagaa
     4321 ggcgtgcaga tcaccgagag cggcaagttt catgtggacg acgagggaac cctgaccatc
     4381 tacgatgccg gctttccaga ccagggcaga tacgagtgtg tggcccggaa ttcttttggc
     4441 ctggccgtga ccaacatgtt cctgacagtg accgccattc agggcagaca ggccggcgac
     4501 gattttgtgg aaagcagcat tctggacgcc gtgcagagag tggatagcgc catcaatagc
     4561 accagacggc acctgttcag ccagaagcct cacacatcta gcgatctgct ggcccagttt
     4621 cactacccta gggaccctct gatcgtggaa atggccagag ccggcgagat ctttgagcac
     4681 acactgcagc tgatccgcga gagagtgaag cagggactga cagtggatct ggaaggcaaa
     4741 gagttccggt acaacgacct ggtgtcccct agaagcctga gcctgatcgc caatctgagc
     4801 ggatgtactg ccagaaggcc cctgcctaac tgcagcaata gatgcttcca cgccaagtac
     4861 agagcccacg acggaacctg caacaacctc cagcaaccta catggggagc cgctctgact
     4921 gcctttgcta gactgctcca gccagcctac agagatggaa tcagagcacc tagaggcctg
     4981 ggactgcctg ttggaagtag acaacctctg cctcctccta gactggtggc cacagtttgg
     5041 gctagagctg ccgctgtgac acccgatcac agctacacaa gaatgctgat gcactggggc
     5101 tggtttctgg aacacgatct ggaccatacc gtgcctgctc tgagcaccgc cagattttcc
     5161 gatggcagac cttgcagcag cgtgtgcacc aacgatcctc catgcttccc catgaacacc
     5221 cggcacgctg atcctagagg aacacacgcc ccttgcatgc tgtttgccag atcttctcct
     5281 gcctgtgcct ctggcagacc aagcgctaca gtggattctg tgtacgccag agagcagatc
     5341 aaccagcaga ccgcctacat cgacggcagc aatgtgtacg gcagcagcga gagagaatct
     5401 caggcactga gagatcctag cgtgcccaga ggcctgctga aaacaggatt tccttggcca
     5461 cctagcggca agcccctgct gccattttct acaggccctc caaccgagtg cgccagacaa
     5521 gagcaagaga gcccttgttt tctggccggc gatcacagag ccaatgagca tctggcactg
     5581 gccgccatgc acaccctgtg gttcagagaa cacaaccgga tggccaccga gctgagcgca
     5641 ctgaatcctc actgggaggg aaataccgtg taccaagagg ccagaaagat cgtgggagcc
     5701 gagctgcagc acattaccta ctctcactgg ctgcccaagg tgctgggcga tcctggaact
     5761 agaatgctca gaggctaccg gggctacaac cccaatgtga acgccggcat catcaacagc
     5821 tttgccaccg ccgccttcag attcggacac accctgatca accccatcct gtacagactg
     5881 aacgccacac tgggagagat cagcgagggc catctgcctt tccataaggc cctgtttagc
     5941 cccagcagaa tcatcaaaga aggtggcatc gaccccgtgc tgagaggact gtttggagtg
     6001 gctgctaagt ggcgggctcc tagctatctc ctgtctcctg agctgaccca gagactgttt
     6061 agcgccgcct attctgctgc tgtggattcc gccgccacaa tcatccagag aggcagagat
     6121 cacggcatcc ctccatacgt ggacttcaga gtgttctgca acctgacctc cgtgaagaac
     6181 ttcgaggatc tgcagaatga gatcaaggac agcgagatcc ggcagaagct gcggaagctt
     6241 tacggcagcc ctggcgatat tgatctgtgg ccagcactga tggtggaaga tctgatccct
     6301 ggcacaagag tgggccccac actgatgtgt ctgttcgtga cacagttcca gcggctgcgc
     6361 gacggcgata gattttggta cgagaaccct ggcgtgttca cccctgctca gctgacacag
     6421 ctgaaacagg cttccctgag cagagtgctg tgcgacaatg gcgatagcat ccagcaggtt
     6481 caggccgacg tgttcgtgaa ggccgagtat ccccaggact acctgaactg cagcgagatt
     6541 cccaaggtgg acctgagagt gtggcaggac tgttgcgccg attgcagaag cagaggccag
     6601 ttcagagcag tgacccaaga gtcccagaag aagagaagcg cccagtacag ctaccccgtg
     6661 gacaaggaca tggaactgag ccacctgaga agcaggcagc aggacaaaat ctacgtgggc
     6721 gaagatgccc ggaatgtgac tgtgctggct aagaccaagt tctcccagga cttcagcacc
     6781 ttcgccgccg agatccaaga aaccatcaca gccctgcggg aacagattaa caagctggaa
     6841 gccagactga ggcaggccgg ttgtacagat gttcggggag tgcctcggaa ggccgaagaa
     6901 cggtggatga aggaagattg cacccactgc atctgcgagt ccggccaagt gacctgcgtg
     6961 gtggaaattt gccctcctgc tccatgtcca tcacctgagc tggtcaaggg cacatgctgc
     7021 cccgtgtgta gagatagagg catgccaagc gacagccccg agaagcgagg cgcgccggaa
     7081 gatcaagttg atccaagatt gattgatggc aagggcggat ctctggaggt gctgttccag
     7141 ggaccaggcg gaagcggcaa tcccggcggc gatctggccc cccagatgct gcgcgagctg
     7201 caggagacca atgccgccct gcaggacgtg cgcgagctgc tgcgccaccg cgtgaaggag
     7261 atcaccttcc tgaagaacac cgtgatggag tgcgacgcct gcggtatgca gccctctaga
     7321 ggctccggct ccatcatccc agttgaggag gagaacccgg acttctggaa ccgcgaggca
     7381 gccgaggccc tgggtgccgc caagaagctg cagcctgcac agacagccgc caagaacctc
     7441 atcatcttcc tgggcgatgg gatgggggtg tctacggtga cagctgccag gatcctaaaa
     7501 gggcagaaga aggacaaact ggggcctgag atacccctgg ccatggaccg cttcccatat
     7561 gtggctctgt ccaagacata caatgtagac aaacatgtgc cagacagtgg agccacagcc
     7621 acggcctacc tgtgcggggt caagggcaac ttccagacca ttggcttgag tgcagccgcc
     7681 cgctttaacc agtgcaacac gacacgcggc aacgaggtca tctccgtgat gaatcgggcc
     7741 aagaaagcag ggaagtcagt gggagtggta accaccacac gagtgcagca cgcctcgcca
     7801 gccggcacct acgcccacac ggtgaaccgc aactggtact cggacgccga cgtgcctgcc
     7861 tcggcccgcc aggaggggtg ccaggacatc gctacgcagc tcatctccaa catggacatt
     7921 gacgtgatcc taggtggagg ccgaaagtac atgtttcgca tgggaacccc agaccctgag
     7981 tacccagatg actacagcca aggtgggacc aggctggacg ggaagaatct ggtgcaggaa
     8041 tggctggcga agcgccaggg tgcccggtat gtgtggaacc gcactgagct catgcaggct
     8101 tccctggacc cgtctgtgac ccatctcatg ggtctctttg agcctggaga catgaaatac
     8161 gagatccacc gagactccac actggacccc tccctgatgg agatgacaga ggctgccctg
     8221 cgcctgctga gcaggaaccc ccgcggcttc ttcctcttcg tggagggtgg tcgcatcgac
     8281 catggtcatc atgaaagcag ggcttaccgg gcactgactg agacgatcat gttcgacgac
     8341 gccattgaga gggcgggcca gctcaccagc gaggaggaca cgctgagcct cgtcactgcc
     8401 gaccactccc acgtcttctc cttcggaggc taccccctgc gagggagctc catcttcggg
     8461 ctggcccctg gcaaggcccg ggacaggaag gcctacacgg tcctcctata cggaaacggt
     8521 ccaggctatg tgctcaagga cggcgcccgg ccggatgtta ccgagagcga gagcgggagc
     8581 cccgagtatc ggcagcagtc agcagtgccc ctggacgaag agacccacgc aggcgaggac
     8641 gtggcggtgt tcgcgcgcgg cccgcaggcg cacctggttc acggcgtgca ggagcagacc
     8701 ttcatagcgc acgtcatggc cttcgccgcc tgcctggagc cctacaccgc ctgcgacctg
     8761 gcgccccccg ccggcaccac cgacgccgcg cacccgggtg gcggaggact gaacgacatc
     8821 ttcgaggctc agaaaatcga atggcacgaa catcaccatc accatcacca tcaccattaa
     8881 ggccggcctg tagtcactgg cacaacgcgt tacccggtaa gccaatcggg tatacacggt
     8941 cgtcatactg cagacagggt tcttctactt tgcaagatag tcttgagtag taaaataaat
     9001 agatagagaa aatcagcctc gactgtgcct tctagttgcc agccatctgt tgtttgcccc
     9061 tcccccgtgc cttccttgac cctggaaggt gccactccca ctgtcctttc ctaataaaat
     9121 gaggaaattg catcacaaca ctcaacccta tctcggtcta ttcttttgat ttataaggga
     9181 ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga
     9241 attaattctg tggaatgtgt gtcagttagg gtgtggaaag tccccaggct ccccagcagg
     9301 cagaagtatg caaagcatgc atctcaatta gtcagcaacc aggtgtggaa agtccccagg
     9361 ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc
     9421 gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca
     9481 tggctgacta atttttttta tttatgcaga ggccgaggcc gcctctgcct ctgagctatt
     9541 ccagaagtag tgaggaggct tttttggagg cctaggcttt tgcaaaaagc tcccgggagc
     9601 ttgtatatcc attttcggat ctgatcagca cgtgttgaca attaatcatc ggcatagtat
     9661 atcggcatag tataatacga caaggtgagg aactaaacca tgaccgagta caagcccacg
     9721 gtgcgcctcg ccacccgcga cgacgtcccc agggccgtac gcaccctcgc cgccgcgttc
     9781 gccgactacc ccgccacgcg ccacaccgtc gatccggacc gccacatcga gcgggtcacc
     9841 gagctgcaag aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg
     9901 gacgacggcg ccgcggtggc ggtctggacc acgccggaga gcgtcgaagc gggggcggtg
     9961 ttcgccgaga tcggcccgcg catggccgag ttgagcggtt cccggctggc cgcgcagcaa
    10021 cagatggaag gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc
    10081 gtcggcgtct cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt gctccccgga
    10141 gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg agacatccgc gccccgcaac
    10201 ctccccttct acgagcggct cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga
    10261 ccgcgcacct ggtgcatgac ccgcaagccc ggtgcctgac acgtgctacg agatttcgat
    10321 tccaccgccg ccttctatga aaggttgggc ttcggaatcg ttttccggga cgccggctgg
    10381 atgatcctcc agcgcgggga tctcatgctg gagttcttcg cccaccccaa cttgtttatt
    10441 gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt
    10501 ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgt
    10561 ataccgtcga cctctagcta gagcttggcg taatcatggt cattaccaat gcttaatcag
    10621 tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt
    10681 cgtgtagata actacgatac gggagggctt accatctggc cccagcgctg cgatgatacc
    10741 gcgagaacca cgctcaccgg ctccggattt atcagcaata aaccagccag ccggaagggc
    10801 cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg
    10861 ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccatcgctac
    10921 aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg
    10981 atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc
    11041 tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact
    11101 gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc
    11161 aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat
    11221 acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc
    11281 ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac
    11341 tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa
    11401 aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact
    11461 catattcttc ctt
//
