LOCUS       sequence-304321-       11512 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of cell-surface protein partial extracellular 
            domain fused to COMP5AP-AviTag-9xHis. Protein is secreted from 
            cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11512)
  AUTHORS   Wojtowicz WM, Vielmetter J, Fernandes RA, Siepe DH, Eastman CL, 
            Chisholm GB, Cox S, Klock H, Anderson PW, Rue SM, Miller JJ, Glaser 
            SM, Bragstad ML, Vance J, Lam AW, Lesley SA, Zinn K, Garcia KC
  TITLE     A Human IgSF Cell-Surface Interactome Reveals a Complex Network of 
            Protein-Protein Interactions.
  JOURNAL   Cell. 2020 Aug 20;182(4):1027-1043.e17. doi: 
            10.1016/j.cell.2020.07.025.
  PUBMED    32822567
REFERENCE   2  (bases 1 to 11512)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2020.07"; journalName: "Cell"; date: "2020-08-20- 
            20"; volume: "182"; issue: "4"; pages: "1027-1043.e17"
COMMENT     Sequence Label: pD649-HAsp-PXDN(Trunc1)-COMP5AP-AviTag-9xHis
FEATURES             Location/Qualifiers
     source          1..11512
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     124..143
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     149..168
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     rep_origin      254..842
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     743..762
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     996..1013
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     1111..1128
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     1112..1128
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    1584..1600
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     enhancer        1767..2146
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2147..2350
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2301..2321
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     2347..2371
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        2581..2599
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     2581..2598
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     2612..2631
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     CDS             7117..7152
                     /codon_start=1
                     /product="epitope tag derived from human Protein C (Stearns
                     et al., 1988)"
                     /label=Protein C tag
                     /translation="EDQVDPRLIDGK"
     CDS             7162..7185
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     CDS             8845..8889
                     /codon_start=1
                     /product="peptide tag that allows for enzymatic 
                     biotinylation"
                     /label=AviTag(TM)
                     /translation="GLNDIFEAQKIEWHE"
     CDS             8890..8916
                     /codon_start=1
                     /product="9xHis affinity tag"
                     /label=9xHis
                     /translation="HHHHHHHHH"
     primer_bind     complement(9056..9073)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    9062..9173
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(9291..9311)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        9296..9625
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      9476..9611
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     9538..9557
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        9673..9720
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             9739..10338
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(9739..9758)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     10235..10255
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     polyA_signal    10468..10589
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10505..10524)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10559..10578
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     CDS             complement(join(10642..11433,11434..11502))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11265..11284
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(join(11503..11512,1..82))
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa
       61 tgtatttaga aaaataaaca aataggggtc agtgttacaa ccaattaacc aattctgaac
      121 attatcgcga gcccatttat acctgaatat ggctcataac accccttgct catgaccaaa
      181 atcccttaac gtgagttacg cgcgcgtcgt tccactgagc gtcagacccc gtagaaaaga
      241 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa
      301 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga
      361 aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt
      421 tagcccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt
      481 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat
      541 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct
      601 tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca
      661 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
      721 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
      781 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
      841 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
      901 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
      961 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg
     1021 aaggcgagag tagggaactg ccaggcatca aactaagcag aaggcccctg acggatggcc
     1081 tttttgcgtt tctacaaact ctttctgtgt tgtaaaacga cggccagtct taagctcggg
     1141 ccccctgggc ggttctgata acgagtaatc gttaatccgc aaataacgta aaaacccgct
     1201 tcggcgggtt tttttatggg gggagtttag ggaaagagca tttgtcagaa tatttaaggg
     1261 cgcctgtcac tttgcttgat atatgagaat tatttaacct tataaatgag aaaaaagcaa
     1321 cgcactttaa ataagatacg ttgctttttc gattgatgaa cacctataat taaactattc
     1381 atctattatt tatgattttt tgtatataca atatttctag tttgttaaag agaattaaga
     1441 aaataaatct cgaaaataat aaagggaaaa tcagtttttg atatcaaaat tatacatgtc
     1501 aacgataata caaaatataa tacaaactat aagatgttat cagtatttat tatcatttag
     1561 aataaatttt gtgtcgccct taattgtgag cggataacaa ttacgagctt catgcacagt
     1621 ggcgttgact caatattggc cattagccat attattcatt ggttatatag cataaatcaa
     1681 tattggctat tggccattgc atacgttgta tctatatcat aatatgtaca tttatattgg
     1741 ctcatgtcca atatgaccgc catgttggca ttgattattg actagttatt aatagtaatc
     1801 aattacgggg tcattagttc atagcccata tatggagttc cgcgttacat aacttacggt
     1861 aaatggcccg cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta
     1921 tgttcccata gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg
     1981 gtaaactgcc cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga
     2041 cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag tacatgacct tatgggactt
     2101 tcctacttgg cagtacatct acgtattagt catcgctatt accatggtga tgcggttttg
     2161 gcagtacatc aatgggcgtg gatagcggtt tgactcacgg ggatttccaa gtctccaccc
     2221 cattgacgtc aatgggagtt tgttttggca ccaaaatcaa cgggactttc caaaatgtcg
     2281 taacaactcc gccccattga cgcaaatggg cggtaggcgt gtacggtggg aggtctatat
     2341 aagcagagct cgtttagtga accgtcagat cgcctggaga cgccatccac gctgttttga
     2401 cctccataga agacaccggg accgatccag cctccgcggc cgggaacggt gcattggaac
     2461 gcggattccc cgtgccaaga gtgacgtaag taccgcctat agagtctata ggcccacccc
     2521 cttggcttcg ttagaacgcg gctacaatta atacataacc ttatgtatca tacacatacg
     2581 atttaggtga cactatagaa taacatccac tttgcctttc tctccacagg tgtccactcc
     2641 caggtccaac tgcacctcgg ttctatcgaa aacgcgcctg ctagcgccac catgaagacc
     2701 atcattgccc tgagctacat cttctgcctg gtgttcgctg cggccgccgt tgttgctcaa
     2761 aaacctggcg ctggctgccc tagcagatgc ctgtgtttta gaaccaccgt gcggtgcatg
     2821 catctgctgc tggaagctgt tcctgccgtg gctcctcaga cctccatcct ggacctgcgg
     2881 ttcaaccgga tcagagaaat ccagcctggc gccttcagac ggctgagaaa cctgaatacc
     2941 ctgctgctga acaacaacca gatcaagaga atccccagcg gcgccttcga ggacctggaa
     3001 aatctgaagt acctgtacct ctacaagaac gagatccaga gcatcgaccg gcaggccttc
     3061 aaaggactgg cttccctgga acagctgtac ctgcacttca atcagatcga gacactggac
     3121 cccgacagct ttcagcatct gcccaagctg gaacggctgt ttctgcacaa caacagaatc
     3181 acccacctgg tgccaggcac cttcaaccac ctggaatcca tgaagcggct gcggctggat
     3241 agcaacaccc tgcactgtga ttgcgagatc ctgtggctgg ccgacctgct gaaaacatac
     3301 gccgagtctg gaaatgccca ggccgctgcc atctgcgagt accctagaag aatccagggc
     3361 agaagcgtgg ccaccatcac acccgaggaa ctgaattgcg agaggcccag aatcacaagc
     3421 gagccccagg atgccgatgt gacaagcggc aacaccgtgt acttcacctg tagagccgag
     3481 ggcaacccca agcctgagat catctggctg cgcaacaaca acgagctgag catgaagacc
     3541 gacagcagac tgaacctgct ggacgacggc accctgatga tccagaacac ccaagagaca
     3601 gaccagggca tctaccagtg catggccaag aatgtggccg gcgaagtgaa aacccaagaa
     3661 gtgaccctga gatacttcgg cagccccgcc agacctacct ttgtgatcca gcctcagaac
     3721 accgaggtgc tcgtgggaga gtctgtgaca ctggaatgta gcgccacagg acaccctcca
     3781 cctagaatca gctggaccag aggcgataga acccctctgc ctgtggaccc cagagtgaat
     3841 atcacacctt ctggcggcct gtacatccag aacgtggtgc aaggcgatag cggcgagtat
     3901 gcctgcagcg ccaccaacaa tatcgattct gtgcacgcca ccgccttcat cattgtgcag
     3961 gccctgcctc agttcaccgt gacacctcag gatagagtgg tcatcgaggg ccagaccgtg
     4021 gattttcagt gcgaggccaa gggcaatcct cctcctgtga tcgcttggac aaaaggcggc
     4081 tcccagctgt ccgtggatag aaggcatctg gtgctgagca gcggcacact gagaattagc
     4141 ggagtggccc tgcacgatca gggccagtat gagtgtcagg ccgtgaacat catcggcagc
     4201 cagaaagtgg tggcccacct gacagtgcag cctagagtga cccctgtgtt cgccagcatt
     4261 cctagcgata ccacagtgga agtgggcgcc aatgtgcagc tgccttgttc tagtcagggc
     4321 gagcctgagc ctgccatcac atggaacaaa gacggcgtgc aagtgaccga gagcggcaag
     4381 tttcatatca gccctgaggg cttcctgacc atcaatgatg tgggacctgc cgacgccggc
     4441 agatatgagt gtgtggccag aaataccatc ggctccgcct ccgtgtctat ggtgctgtct
     4501 gtgaatgtgc ccgacgtgtc cagaaacggc gatccttttg tggccacctc tatcgtggaa
     4561 gccattgcca ccgtggaccg ggccatcaat agcaccagaa cacacctgtt cgacagccgg
     4621 cctagaagcc ctaatgacct gctggccctg ttcagatacc ccagagatcc ctataccgtg
     4681 gaacaggcca gagccggcga gatctttgag agaaccctgc agctgatcca agagcacgtg
     4741 cagcacggac tgatggtgga tctgaatggc accagctacc actacaacga cctggtgtcc
     4801 ccacagtacc tgaacctgat cgccaatctg tctggctgca cagcccacag aagagtgaac
     4861 aactgcagcg acatgtgctt ccaccagaag tacagaaccc acgacggaac ctgcaacaac
     4921 ctgcagcatc ctatgtgggg agccagcctg accgcctttg aaagactgct gaagtccgtg
     4981 tacgagaacg gcttcaacac ccctagaggc atcaaccctc accggctgta caatggacat
     5041 gccctgccta tgcctcggct ggtgtctaca acactgatcg gcaccgagac agtgacccca
     5101 gatgagcagt tcacccacat gctgatgcag tggggccagt tcctggatca cgatctggat
     5161 tctaccgtgg tggctctgag ccaggccaga ttttctgatg gccagcactg cagcaacgtg
     5221 tgcagcaatg atcctccttg cttcagcgtg atgatccctc ctaacgactc cagagctaga
     5281 agcggagccc ggtgcatgtt ttttgtgcgg tctagccctg tgtgcggcag cggaatgaca
     5341 agcctgctga tgaatagcgt gtaccccagg gaacagatca accagctgac ctcctacatc
     5401 gacgcctcca atgtgtacgg cagcacagag cacgaggccc gctctattag agatctggcc
     5461 tctcacagag gcctgctgag acagggaatt gtgcagagaa gcggcaagcc tctgctgcct
     5521 tttgccaccg gacctccaac cgagtgcatg agagatgaga acgagagccc cattccttgc
     5581 tttctggccg gggatcacag agccaatgaa cagctgggcc tgaccagcat gcacaccctg
     5641 tggttcagag agcacaatcg gatcgccaca gagctgctga aactgaaccc tcactgggac
     5701 ggcgacacca tctactacga gacaagaaag atcgtgggcg ccgagattca gcacatcacc
     5761 tatcagcact ggctgcctaa gatcctgggc gaagtgggca tgagaacact gggcgagtac
     5821 cacggctacg accctggaat caacgccggc atctttaacg ccttcgccac cgctgccttc
     5881 agattcggac acaccctggt caaccctctg ctgtaccggc tggacgagaa cttccagcct
     5941 atcgctcagg accatctgcc actgcacaag gcctttttca gccccttccg gatcgtgaac
     6001 gaaggcggaa tcgatccact gctgagaggc ctgtttggcg tggccggaaa gatgagagtt
     6061 cccagccagc tgctcaatac cgagctgacc gaaagactgt tcagcatggc ccacactgtg
     6121 gccctggatc tggccgccat taacatccag agaggcagag atcacggcat ccctccatac
     6181 cacgactacc gggtgtactg caatctgagc gccgctcaca cctttgagga tctgaagaat
     6241 gagatcaaga accccgagat cagagagaag ctgaagagac tgtacggctc taccctgaac
     6301 atcgatctgt ttcccgctct ggtggtggaa gatctggtgc ctggaagcag actgggccct
     6361 acactgatgt gtctgctgag cacccagttc aagcggctga gagatggcga ccggctttgg
     6421 tacgaaaacc ctggcgtgtt cagccctgct cagctgaccc agattaagca gactagcctg
     6481 gccagaatcc tgtgcgacaa cgccgacaac atcaccagag tgcagagcga cgtgttcaga
     6541 gtggccgagt ttcctcacgg ctatggcagc tgtgacgaga tcccaagagt ggacctgaga
     6601 gtgtggcagg actgctgcga ggattgcaga accagaggcc agttcaacgc cttcagctat
     6661 cacttccggg gcagaagaag cctggaattc agctaccaag aggacaagcc caccaagaaa
     6721 acccggccta ggaagatccc ttctgtcggc agacagggcg aacacctgag caattctacc
     6781 agcgccttct ccaccagatc cgacgccagc ggaacaaacg acttccgcga gttcgtgctc
     6841 gagatgcaga aaaccatcac cgacctgcgg acccagatca aaaagctgga aagcaggctg
     6901 tccaccaccg aatgtgtgga tgctggcgga gagtcccacg ccaacaacac caagtggaag
     6961 aaggacgcct gcaccatttg cgagtgcaag gatggccaag tgacctgctt tgtggaagcc
     7021 tgtcctcctg ctacatgcgc cgtgcctgtt aatatccctg gcgcttgttg tcccgtgtgc
     7081 ctgcagaaga gagccgaaga gaaacctggc gcgccggaag atcaagttga tccaagattg
     7141 attgatggca agggcggatc tctggaggtg ctgttccagg gaccaggcgg aagcggcaat
     7201 cccggcggcg atctggcccc ccagatgctg cgcgagctgc aggagaccaa tgccgccctg
     7261 caggacgtgc gcgagctgct gcgccaccgc gtgaaggaga tcaccttcct gaagaacacc
     7321 gtgatggagt gcgacgcctg cggtatgcag ccctctagag gctccggctc catcatccca
     7381 gttgaggagg agaacccgga cttctggaac cgcgaggcag ccgaggccct gggtgccgcc
     7441 aagaagctgc agcctgcaca gacagccgcc aagaacctca tcatcttcct gggcgatggg
     7501 atgggggtgt ctacggtgac agctgccagg atcctaaaag ggcagaagaa ggacaaactg
     7561 gggcctgaga tacccctggc catggaccgc ttcccatatg tggctctgtc caagacatac
     7621 aatgtagaca aacatgtgcc agacagtgga gccacagcca cggcctacct gtgcggggtc
     7681 aagggcaact tccagaccat tggcttgagt gcagccgccc gctttaacca gtgcaacacg
     7741 acacgcggca acgaggtcat ctccgtgatg aatcgggcca agaaagcagg gaagtcagtg
     7801 ggagtggtaa ccaccacacg agtgcagcac gcctcgccag ccggcaccta cgcccacacg
     7861 gtgaaccgca actggtactc ggacgccgac gtgcctgcct cggcccgcca ggaggggtgc
     7921 caggacatcg ctacgcagct catctccaac atggacattg acgtgatcct aggtggaggc
     7981 cgaaagtaca tgtttcgcat gggaacccca gaccctgagt acccagatga ctacagccaa
     8041 ggtgggacca ggctggacgg gaagaatctg gtgcaggaat ggctggcgaa gcgccagggt
     8101 gcccggtatg tgtggaaccg cactgagctc atgcaggctt ccctggaccc gtctgtgacc
     8161 catctcatgg gtctctttga gcctggagac atgaaatacg agatccaccg agactccaca
     8221 ctggacccct ccctgatgga gatgacagag gctgccctgc gcctgctgag caggaacccc
     8281 cgcggcttct tcctcttcgt ggagggtggt cgcatcgacc atggtcatca tgaaagcagg
     8341 gcttaccggg cactgactga gacgatcatg ttcgacgacg ccattgagag ggcgggccag
     8401 ctcaccagcg aggaggacac gctgagcctc gtcactgccg accactccca cgtcttctcc
     8461 ttcggaggct accccctgcg agggagctcc atcttcgggc tggcccctgg caaggcccgg
     8521 gacaggaagg cctacacggt cctcctatac ggaaacggtc caggctatgt gctcaaggac
     8581 ggcgcccggc cggatgttac cgagagcgag agcgggagcc ccgagtatcg gcagcagtca
     8641 gcagtgcccc tggacgaaga gacccacgca ggcgaggacg tggcggtgtt cgcgcgcggc
     8701 ccgcaggcgc acctggttca cggcgtgcag gagcagacct tcatagcgca cgtcatggcc
     8761 ttcgccgcct gcctggagcc ctacaccgcc tgcgacctgg cgccccccgc cggcaccacc
     8821 gacgccgcgc acccgggtgg cggaggactg aacgacatct tcgaggctca gaaaatcgaa
     8881 tggcacgaac atcaccatca ccatcaccat caccattaag gccggcctgt agtcactggc
     8941 acaacgcgtt acccggtaag ccaatcgggt atacacggtc gtcatactgc agacagggtt
     9001 cttctacttt gcaagatagt cttgagtagt aaaataaata gatagagaaa atcagcctcg
     9061 actgtgcctt ctagttgcca gccatctgtt gtttgcccct cccccgtgcc ttccttgacc
     9121 ctggaaggtg ccactcccac tgtcctttcc taataaaatg aggaaattgc atcacaacac
     9181 tcaaccctat ctcggtctat tcttttgatt tataagggat tttgccgatt tcggcctatt
     9241 ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttaattctgt ggaatgtgtg
     9301 tcagttaggg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca
     9361 tctcaattag tcagcaacca ggtgtggaaa gtccccaggc tccccagcag gcagaagtat
     9421 gcaaagcatg catctcaatt agtcagcaac catagtcccg cccctaactc cgcccatccc
     9481 gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat
     9541 ttatgcagag gccgaggccg cctctgcctc tgagctattc cagaagtagt gaggaggctt
     9601 ttttggaggc ctaggctttt gcaaaaagct cccgggagct tgtatatcca ttttcggatc
     9661 tgatcagcac gtgttgacaa ttaatcatcg gcatagtata tcggcatagt ataatacgac
     9721 aaggtgagga actaaaccat gaccgagtac aagcccacgg tgcgcctcgc cacccgcgac
     9781 gacgtcccca gggccgtacg caccctcgcc gccgcgttcg ccgactaccc cgccacgcgc
     9841 cacaccgtcg atccggaccg ccacatcgag cgggtcaccg agctgcaaga actcttcctc
     9901 acgcgcgtcg ggctcgacat cggcaaggtg tgggtcgcgg acgacggcgc cgcggtggcg
     9961 gtctggacca cgccggagag cgtcgaagcg ggggcggtgt tcgccgagat cggcccgcgc
    10021 atggccgagt tgagcggttc ccggctggcc gcgcagcaac agatggaagg cctcctggcg
    10081 ccgcaccggc ccaaggagcc cgcgtggttc ctggccaccg tcggcgtctc gcccgaccac
    10141 cagggcaagg gtctgggcag cgccgtcgtg ctccccggag tggaggcggc cgagcgcgcc
    10201 ggggtgcccg ccttcctgga gacatccgcg ccccgcaacc tccccttcta cgagcggctc
    10261 ggcttcaccg tcaccgccga cgtcgaggtg cccgaaggac cgcgcacctg gtgcatgacc
    10321 cgcaagcccg gtgcctgaca cgtgctacga gatttcgatt ccaccgccgc cttctatgaa
    10381 aggttgggct tcggaatcgt tttccgggac gccggctgga tgatcctcca gcgcggggat
    10441 ctcatgctgg agttcttcgc ccaccccaac ttgtttattg cagcttataa tggttacaaa
    10501 taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt
    10561 ggtttgtcca aactcatcaa tgtatcttat catgtctgta taccgtcgac ctctagctag
    10621 agcttggcgt aatcatggtc attaccaatg cttaatcagt gaggcaccta tctcagcgat
    10681 ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg
    10741 ggagggctta ccatctggcc ccagcgctgc gatgataccg cgagaaccac gctcaccggc
    10801 tccggattta tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc
    10861 aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc
    10921 gccagttaat agtttgcgca acgttgttgc catcgctaca ggcatcgtgg tgtcacgctc
    10981 gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc
    11041 ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa
    11101 gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat
    11161 gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata
    11221 gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca
    11281 tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag
    11341 gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc
    11401 agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc
    11461 aaaaaaggga ataagggcga cacggaaatg ttgaatactc atattcttcc tt
//
