LOCUS       sequence-304323-       11872 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of cell-surface protein partial extracellular 
            domain fused to COMP5AP-AviTag-9xHis. Protein is secreted from 
            cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11872)
  AUTHORS   Wojtowicz WM, Vielmetter J, Fernandes RA, Siepe DH, Eastman CL, 
            Chisholm GB, Cox S, Klock H, Anderson PW, Rue SM, Miller JJ, Glaser 
            SM, Bragstad ML, Vance J, Lam AW, Lesley SA, Zinn K, Garcia KC
  TITLE     A Human IgSF Cell-Surface Interactome Reveals a Complex Network of 
            Protein-Protein Interactions.
  JOURNAL   Cell. 2020 Aug 20;182(4):1027-1043.e17. doi: 
            10.1016/j.cell.2020.07.025.
  PUBMED    32822567
REFERENCE   2  (bases 1 to 11872)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2020.07"; journalName: "Cell"; date: "2020-08-20- 
            20"; volume: "182"; issue: "4"; pages: "1027-1043.e17"
COMMENT     Sequence Label: pD649-HAsp-DSCAML1(Trunc1)-COMP5AP-AviTag-9xHis
FEATURES             Location/Qualifiers
     source          1..11872
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     124..143
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     149..168
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     rep_origin      254..842
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     743..762
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     996..1013
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     1111..1128
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     1112..1128
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    1584..1600
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     enhancer        1767..2146
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2147..2350
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2301..2321
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     2347..2371
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        2581..2599
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     2581..2598
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     2612..2631
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     CDS             7477..7512
                     /codon_start=1
                     /product="epitope tag derived from human Protein C (Stearns
                     et al., 1988)"
                     /label=Protein C tag
                     /translation="EDQVDPRLIDGK"
     CDS             7522..7545
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     CDS             9205..9249
                     /codon_start=1
                     /product="peptide tag that allows for enzymatic 
                     biotinylation"
                     /label=AviTag(TM)
                     /translation="GLNDIFEAQKIEWHE"
     CDS             9250..9276
                     /codon_start=1
                     /product="9xHis affinity tag"
                     /label=9xHis
                     /translation="HHHHHHHHH"
     primer_bind     complement(9416..9433)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    9422..9533
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(9651..9671)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        9656..9985
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      9836..9971
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     9898..9917
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        10033..10080
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             10099..10698
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(10099..10118)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     10595..10615
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     polyA_signal    10828..10949
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10865..10884)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10919..10938
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     CDS             complement(join(11002..11793,11794..11862))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11625..11644
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(join(11863..11872,1..82))
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa
       61 tgtatttaga aaaataaaca aataggggtc agtgttacaa ccaattaacc aattctgaac
      121 attatcgcga gcccatttat acctgaatat ggctcataac accccttgct catgaccaaa
      181 atcccttaac gtgagttacg cgcgcgtcgt tccactgagc gtcagacccc gtagaaaaga
      241 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa
      301 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga
      361 aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt
      421 tagcccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt
      481 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat
      541 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct
      601 tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca
      661 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
      721 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
      781 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
      841 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
      901 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
      961 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg
     1021 aaggcgagag tagggaactg ccaggcatca aactaagcag aaggcccctg acggatggcc
     1081 tttttgcgtt tctacaaact ctttctgtgt tgtaaaacga cggccagtct taagctcggg
     1141 ccccctgggc ggttctgata acgagtaatc gttaatccgc aaataacgta aaaacccgct
     1201 tcggcgggtt tttttatggg gggagtttag ggaaagagca tttgtcagaa tatttaaggg
     1261 cgcctgtcac tttgcttgat atatgagaat tatttaacct tataaatgag aaaaaagcaa
     1321 cgcactttaa ataagatacg ttgctttttc gattgatgaa cacctataat taaactattc
     1381 atctattatt tatgattttt tgtatataca atatttctag tttgttaaag agaattaaga
     1441 aaataaatct cgaaaataat aaagggaaaa tcagtttttg atatcaaaat tatacatgtc
     1501 aacgataata caaaatataa tacaaactat aagatgttat cagtatttat tatcatttag
     1561 aataaatttt gtgtcgccct taattgtgag cggataacaa ttacgagctt catgcacagt
     1621 ggcgttgact caatattggc cattagccat attattcatt ggttatatag cataaatcaa
     1681 tattggctat tggccattgc atacgttgta tctatatcat aatatgtaca tttatattgg
     1741 ctcatgtcca atatgaccgc catgttggca ttgattattg actagttatt aatagtaatc
     1801 aattacgggg tcattagttc atagcccata tatggagttc cgcgttacat aacttacggt
     1861 aaatggcccg cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta
     1921 tgttcccata gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg
     1981 gtaaactgcc cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga
     2041 cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag tacatgacct tatgggactt
     2101 tcctacttgg cagtacatct acgtattagt catcgctatt accatggtga tgcggttttg
     2161 gcagtacatc aatgggcgtg gatagcggtt tgactcacgg ggatttccaa gtctccaccc
     2221 cattgacgtc aatgggagtt tgttttggca ccaaaatcaa cgggactttc caaaatgtcg
     2281 taacaactcc gccccattga cgcaaatggg cggtaggcgt gtacggtggg aggtctatat
     2341 aagcagagct cgtttagtga accgtcagat cgcctggaga cgccatccac gctgttttga
     2401 cctccataga agacaccggg accgatccag cctccgcggc cgggaacggt gcattggaac
     2461 gcggattccc cgtgccaaga gtgacgtaag taccgcctat agagtctata ggcccacccc
     2521 cttggcttcg ttagaacgcg gctacaatta atacataacc ttatgtatca tacacatacg
     2581 atttaggtga cactatagaa taacatccac tttgcctttc tctccacagg tgtccactcc
     2641 caggtccaac tgcacctcgg ttctatcgaa aacgcgcctg ctagcgccac catgaagacc
     2701 atcattgccc tgagctacat cttctgcctg gtgttcgctg cggccgccga agatgtgggc
     2761 acaagcctgt acttcgtgaa cgacagcctg cagcaagtga cctttagcag ctctgtgggc
     2821 gtcgtggttc cttgtcctgc tgctggatct ccatctgccg ctctgagatg gtatctggcc
     2881 accggcgacg acatctacga tgtgcctcac attagacacg tgcacgccaa tggcaccctg
     2941 cagctgtacc cttttagccc cagcgccttc aacagcttca tccacgacaa cgactatttc
     3001 tgcaccgccg agaacgccgc tggcaagatc agatccccta acatcagagt gaaggccgtg
     3061 ttccgcgagc cttacaccgt cagagtggaa gatcagcgga gcatgagagg caacgtggcc
     3121 gtgtttaagt gtctgatccc cagcagcgtg caagagtacg tgtccgtggt gtcctgggag
     3181 aaagacaccg tgtctatcat ccccgagcac cggttcttca tcacatacca cggcggcctg
     3241 tacatcagcg acgtgcagaa agaagatgcc ctgagcacct accggtgcat caccaagcac
     3301 aagtacagcg gcgagacaag acagagcaat ggcgccagac tgagcgtgac agatcctgcc
     3361 gagagcatcc ctaccatcct ggatggcttc cacagccaag aagtgtgggc cggacatacc
     3421 gtggaactgc cttgtacagc cagcggctac cccattcctg ccatcagatg gctgaaggac
     3481 ggtagacctc tgcctgccga tagccggtgg acaaagagaa tcaccggcct gaccatctcc
     3541 gacctgagaa cagaggatag cggcacctac atctgcgaag tgaccaatac ctttggcagc
     3601 gccgaggcca caggcatcct gatggttatt gaccctctgc acgtgaccct gacacctaag
     3661 aagctgaaaa ccggcatcgg ctccaccgtg atcctgtctt gtgctctgac cggctctccc
     3721 gagttcacca tcaggtggta cagaaacacc gagctggtgc tgcctgacga ggccatctct
     3781 atcagaggcc tgagcaacga gacactgctg atcacaagcg cccagaagtc tcacagcggc
     3841 gcctaccagt gctttgccac aagaaaagcc cagacagccc aggacttcgc catcattgcc
     3901 ctggaagatg gcacccctag aatcgtgtcc agcttcagcg agaaggtggt caaccctggc
     3961 gagcagtttt ctctgatgtg tgccgctaaa ggcgcccctc cacctacagt gacatgggcc
     4021 ctcgatgatg agcccatcgt cagagatggc agccacagga ccaaccagta caccatgtcc
     4081 gatggcacca ccatcagcca catgaacgtg acaggcccac agataagaga tggcggcgtg
     4141 tacagatgca cagccagaaa tctcgtgggc tccgccgagt accaggccag aatcaatgtt
     4201 cggggccctc ctagcatccg ggccatgaga aatattacag ccgtggccgg cagagacacc
     4261 ctgatcaatt gcagagtgat cggctaccct tactacagca tcaagtggta taaggacgct
     4321 ctgctgctgc ccgacaacca cagacaggtg gtgttcgaga acggcacact gaagctgacc
     4381 gatgtccaga aaggcatgga cgagggcgag tacctgtgct ctgttctgat ccagccacag
     4441 ctgagcatca gccagtctgt gcatgtggcc gtgaaggtgc cacctctgat tcagcctttc
     4501 gagttccctc ctgcctctat cggccagctg ctgtacatcc cttgtgtggt gtctagcggc
     4561 gacatgccca tcagaatcac atggcggaag gatggccaag tgatcatcag cggctctggc
     4621 gtgaccatcg agagcaaaga attcatgtct agcctgcaga tctccagcgt gtccctgaag
     4681 cacaacggca actacacctg tatcgccagc aatgccgccg ctaccgttag cagagaaagg
     4741 cagctgatcg tgcgggtgcc accaagattt gtggtgcagc ccaacaacca ggacggcatc
     4801 tatggaaagg ccggcgtgct gaactgtagc gtggacggct atccacctcc taaagtgatg
     4861 tggaagcacg ccaaaggctc cggcaatcct cagcagtatc accctgtgcc actgaccggc
     4921 agaattcaga ttctgcccaa cagcagcctg ctgatccggc acgtgctgga agaggatatc
     4981 ggctactatc tgtgccaggc ctctaacggc gtgggcaccg atatcagcaa gagcatgttc
     5041 ctgaccgtga agatccccgc catgatcacc agccatccta acaccacaat cgccatcaag
     5101 ggccatgcca aagagctgaa ttgcaccgcc agaggcgagc ggcccatcat cattagatgg
     5161 gagaagggcg ataccgtgat cgaccccgac agagtgatga gatacgctat cgccaccaag
     5221 gacaacggcg acgaagtggt gtcaacactg aaactgaagc ccgccgacag aggcgacagc
     5281 gtgttcttta gctgccacgc catcaactcc tacggcgagg atagaggact catccagctg
     5341 acagtgcaag agcctcctga tcctcctgag ctggaaatca gagaagtgaa agcccggtcc
     5401 atgaacctgc ggtggaccca gagattcgac ggcaatagca tcatcaccgg cttcgacatc
     5461 gagtacaaga acaagagcga cagctgggac ttcaagcaga gcaccagaaa catcagcccc
     5521 accatcaacc aggccaacat cgtggatctg caccctgcct ccgtgtacag cattagaatg
     5581 tacagcttca acaagatcgg ccgcagcgag cccagcaaag aactgaccat tagcaccgag
     5641 gaagctgccc ctgacggacc tcctatggat gtgacactgc agcctgtgac cagccagagc
     5701 atccaagtta cctggaaggc ccctaagaaa gaactgcaga acggcgtgat ccggggctac
     5761 cagatcggct atagagagaa cagccctggc agcaacggcc agtacagcat cgtggaaatg
     5821 aaggccactg gcgactccga ggtgtacaca ctggacaacc tgaagaagtt cgcccagtac
     5881 ggcgtggtgg tgcaggcctt taatagagcc ggaacaggcc ctagcagctc cgagatcaat
     5941 gccaccacac tcgaggatgt gcctagccag cctccagaaa atgtgcgggc cctgtctatc
     6001 acctccgatg tggctgtgat ctcttggagc gagcctccta gaagcaccct gaatggggtg
     6061 ctgaaggggt atagagtgat cttctggtcc ctgtatgtgg acggcgagtg gggcgagatg
     6121 cagaacatca ccaccaccag agaaagagtg gaactgagag gcatggaaaa gttcaccaac
     6181 tacagcgtgc aggtcctggc ctatacacaa gctggcgacg gcgtcagatc ctccgtgctg
     6241 tatatccaga ccaaagagga cgtcccagga cctcctgccg gaatcaaagc tgtgccttct
     6301 agcgcctctt ctgtggtggt ttcctggctg cctcctacca agcctaatgg cgtgatcaga
     6361 aagtatacca tcttctgcag cagccccgga agcggacaac cagctcctag cgagtatgag
     6421 acaagccccg agcagctgtt ctaccggatc gcccatctga acagaggcca gcagtacctg
     6481 ctgtgggttg ccgctgttac aagcgccgga agaggcaaca gctctgagaa agtgacaatc
     6541 gagcctgccg gcaaggcccc tgccaagatc atctcttttg gcggcaccgt gaccacacct
     6601 tggatgaagg atgtgcggct gccttgcaac agcgtgggag atcctgctcc tgccgtgaaa
     6661 tggaccaagg atagcgagga cagcgctatc ccagtgtcca tggatggcca cagactgatc
     6721 cacaccaacg gaacactgct gctgagagcc gtgaaagccg aggactccgg ctactacaca
     6781 tgcactgcca ccaataccgg cggcttcgat accatcatcg tgaacctgct ggtccaggtt
     6841 ccacctgacc agcctagact gacagtgtct aagaccagcg cctccagcat caccctgacc
     6901 tggattccag gcgataatgg cggcagctcc atcagaggct ttgtgctgca gtacagcgtg
     6961 gacaacagcg aggaatggaa ggacgtgttc atctccagca gcgagcggag cttcaagctg
     7021 gatagcctga agtgcggcac ctggtacaaa gtgaagctgg ccgccaagaa tagcgtcggc
     7081 agcggaagaa tcagcgagat cattgaggcc aagacacacg gcagagagcc tagcttcagc
     7141 aaggaccagc acctgttcac ccacatcaac agcacccatg ccaggctgaa cctgcaaggc
     7201 tggaacaatg gcggctgtcc tattaccgcc atcgtgctcg agtacagacc taaaggcaca
     7261 tgggcctggc agggcctgag agccaatagc tctggcgaag tgtttctgac cgagctgaga
     7321 gaggccactt ggtacgaact gcggatgaga gcctgtaata gcgccggctg tggcaatgag
     7381 actgcccagt ttgccacact ggattacgac ggcagcacaa tccctccaat caagtctgct
     7441 caaggcgagg gcgacgacgt gaagaaaggc gcgccggaag atcaagttga tccaagattg
     7501 attgatggca agggcggatc tctggaggtg ctgttccagg gaccaggcgg aagcggcaat
     7561 cccggcggcg atctggcccc ccagatgctg cgcgagctgc aggagaccaa tgccgccctg
     7621 caggacgtgc gcgagctgct gcgccaccgc gtgaaggaga tcaccttcct gaagaacacc
     7681 gtgatggagt gcgacgcctg cggtatgcag ccctctagag gctccggctc catcatccca
     7741 gttgaggagg agaacccgga cttctggaac cgcgaggcag ccgaggccct gggtgccgcc
     7801 aagaagctgc agcctgcaca gacagccgcc aagaacctca tcatcttcct gggcgatggg
     7861 atgggggtgt ctacggtgac agctgccagg atcctaaaag ggcagaagaa ggacaaactg
     7921 gggcctgaga tacccctggc catggaccgc ttcccatatg tggctctgtc caagacatac
     7981 aatgtagaca aacatgtgcc agacagtgga gccacagcca cggcctacct gtgcggggtc
     8041 aagggcaact tccagaccat tggcttgagt gcagccgccc gctttaacca gtgcaacacg
     8101 acacgcggca acgaggtcat ctccgtgatg aatcgggcca agaaagcagg gaagtcagtg
     8161 ggagtggtaa ccaccacacg agtgcagcac gcctcgccag ccggcaccta cgcccacacg
     8221 gtgaaccgca actggtactc ggacgccgac gtgcctgcct cggcccgcca ggaggggtgc
     8281 caggacatcg ctacgcagct catctccaac atggacattg acgtgatcct aggtggaggc
     8341 cgaaagtaca tgtttcgcat gggaacccca gaccctgagt acccagatga ctacagccaa
     8401 ggtgggacca ggctggacgg gaagaatctg gtgcaggaat ggctggcgaa gcgccagggt
     8461 gcccggtatg tgtggaaccg cactgagctc atgcaggctt ccctggaccc gtctgtgacc
     8521 catctcatgg gtctctttga gcctggagac atgaaatacg agatccaccg agactccaca
     8581 ctggacccct ccctgatgga gatgacagag gctgccctgc gcctgctgag caggaacccc
     8641 cgcggcttct tcctcttcgt ggagggtggt cgcatcgacc atggtcatca tgaaagcagg
     8701 gcttaccggg cactgactga gacgatcatg ttcgacgacg ccattgagag ggcgggccag
     8761 ctcaccagcg aggaggacac gctgagcctc gtcactgccg accactccca cgtcttctcc
     8821 ttcggaggct accccctgcg agggagctcc atcttcgggc tggcccctgg caaggcccgg
     8881 gacaggaagg cctacacggt cctcctatac ggaaacggtc caggctatgt gctcaaggac
     8941 ggcgcccggc cggatgttac cgagagcgag agcgggagcc ccgagtatcg gcagcagtca
     9001 gcagtgcccc tggacgaaga gacccacgca ggcgaggacg tggcggtgtt cgcgcgcggc
     9061 ccgcaggcgc acctggttca cggcgtgcag gagcagacct tcatagcgca cgtcatggcc
     9121 ttcgccgcct gcctggagcc ctacaccgcc tgcgacctgg cgccccccgc cggcaccacc
     9181 gacgccgcgc acccgggtgg cggaggactg aacgacatct tcgaggctca gaaaatcgaa
     9241 tggcacgaac atcaccatca ccatcaccat caccattaag gccggcctgt agtcactggc
     9301 acaacgcgtt acccggtaag ccaatcgggt atacacggtc gtcatactgc agacagggtt
     9361 cttctacttt gcaagatagt cttgagtagt aaaataaata gatagagaaa atcagcctcg
     9421 actgtgcctt ctagttgcca gccatctgtt gtttgcccct cccccgtgcc ttccttgacc
     9481 ctggaaggtg ccactcccac tgtcctttcc taataaaatg aggaaattgc atcacaacac
     9541 tcaaccctat ctcggtctat tcttttgatt tataagggat tttgccgatt tcggcctatt
     9601 ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttaattctgt ggaatgtgtg
     9661 tcagttaggg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca
     9721 tctcaattag tcagcaacca ggtgtggaaa gtccccaggc tccccagcag gcagaagtat
     9781 gcaaagcatg catctcaatt agtcagcaac catagtcccg cccctaactc cgcccatccc
     9841 gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat
     9901 ttatgcagag gccgaggccg cctctgcctc tgagctattc cagaagtagt gaggaggctt
     9961 ttttggaggc ctaggctttt gcaaaaagct cccgggagct tgtatatcca ttttcggatc
    10021 tgatcagcac gtgttgacaa ttaatcatcg gcatagtata tcggcatagt ataatacgac
    10081 aaggtgagga actaaaccat gaccgagtac aagcccacgg tgcgcctcgc cacccgcgac
    10141 gacgtcccca gggccgtacg caccctcgcc gccgcgttcg ccgactaccc cgccacgcgc
    10201 cacaccgtcg atccggaccg ccacatcgag cgggtcaccg agctgcaaga actcttcctc
    10261 acgcgcgtcg ggctcgacat cggcaaggtg tgggtcgcgg acgacggcgc cgcggtggcg
    10321 gtctggacca cgccggagag cgtcgaagcg ggggcggtgt tcgccgagat cggcccgcgc
    10381 atggccgagt tgagcggttc ccggctggcc gcgcagcaac agatggaagg cctcctggcg
    10441 ccgcaccggc ccaaggagcc cgcgtggttc ctggccaccg tcggcgtctc gcccgaccac
    10501 cagggcaagg gtctgggcag cgccgtcgtg ctccccggag tggaggcggc cgagcgcgcc
    10561 ggggtgcccg ccttcctgga gacatccgcg ccccgcaacc tccccttcta cgagcggctc
    10621 ggcttcaccg tcaccgccga cgtcgaggtg cccgaaggac cgcgcacctg gtgcatgacc
    10681 cgcaagcccg gtgcctgaca cgtgctacga gatttcgatt ccaccgccgc cttctatgaa
    10741 aggttgggct tcggaatcgt tttccgggac gccggctgga tgatcctcca gcgcggggat
    10801 ctcatgctgg agttcttcgc ccaccccaac ttgtttattg cagcttataa tggttacaaa
    10861 taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt
    10921 ggtttgtcca aactcatcaa tgtatcttat catgtctgta taccgtcgac ctctagctag
    10981 agcttggcgt aatcatggtc attaccaatg cttaatcagt gaggcaccta tctcagcgat
    11041 ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg
    11101 ggagggctta ccatctggcc ccagcgctgc gatgataccg cgagaaccac gctcaccggc
    11161 tccggattta tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc
    11221 aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc
    11281 gccagttaat agtttgcgca acgttgttgc catcgctaca ggcatcgtgg tgtcacgctc
    11341 gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc
    11401 ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa
    11461 gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat
    11521 gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata
    11581 gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca
    11641 tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag
    11701 gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc
    11761 agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc
    11821 aaaaaaggga ataagggcga cacggaaatg ttgaatactc atattcttcc tt
//
