LOCUS       sequence-304325-       11887 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of cell-surface protein partial extracellular 
            domain fused to COMP5AP-AviTag-9xHis. Protein is secreted from 
            cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11887)
  AUTHORS   Wojtowicz WM, Vielmetter J, Fernandes RA, Siepe DH, Eastman CL, 
            Chisholm GB, Cox S, Klock H, Anderson PW, Rue SM, Miller JJ, Glaser 
            SM, Bragstad ML, Vance J, Lam AW, Lesley SA, Zinn K, Garcia KC
  TITLE     A Human IgSF Cell-Surface Interactome Reveals a Complex Network of 
            Protein-Protein Interactions.
  JOURNAL   Cell. 2020 Aug 20;182(4):1027-1043.e17. doi: 
            10.1016/j.cell.2020.07.025.
  PUBMED    32822567
REFERENCE   2  (bases 1 to 11887)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2020.07"; journalName: "Cell"; date: "2020-08-20- 
            20"; volume: "182"; issue: "4"; pages: "1027-1043.e17"
COMMENT     Sequence Label: pD649-HAsp-DSCAM(Trunc1)-COMP5AP-AviTag-9xHis
FEATURES             Location/Qualifiers
     source          1..11887
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     124..143
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     149..168
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     rep_origin      254..842
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     743..762
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     996..1013
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     1111..1128
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     1112..1128
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    1584..1600
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     enhancer        1767..2146
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2147..2350
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2301..2321
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     2347..2371
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        2581..2599
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     2581..2598
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     2612..2631
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     CDS             7492..7527
                     /codon_start=1
                     /product="epitope tag derived from human Protein C (Stearns
                     et al., 1988)"
                     /label=Protein C tag
                     /translation="EDQVDPRLIDGK"
     CDS             7537..7560
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     CDS             9220..9264
                     /codon_start=1
                     /product="peptide tag that allows for enzymatic 
                     biotinylation"
                     /label=AviTag(TM)
                     /translation="GLNDIFEAQKIEWHE"
     CDS             9265..9291
                     /codon_start=1
                     /product="9xHis affinity tag"
                     /label=9xHis
                     /translation="HHHHHHHHH"
     primer_bind     complement(9431..9448)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    9437..9548
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(9666..9686)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        9671..10000
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      9851..9986
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     9913..9932
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        10048..10095
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             10114..10713
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(10114..10133)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     10610..10630
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     polyA_signal    10843..10964
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10880..10899)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10934..10953
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     CDS             complement(join(11017..11808,11809..11877))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11640..11659
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(join(11878..11887,1..82))
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa
       61 tgtatttaga aaaataaaca aataggggtc agtgttacaa ccaattaacc aattctgaac
      121 attatcgcga gcccatttat acctgaatat ggctcataac accccttgct catgaccaaa
      181 atcccttaac gtgagttacg cgcgcgtcgt tccactgagc gtcagacccc gtagaaaaga
      241 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa
      301 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga
      361 aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt
      421 tagcccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt
      481 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat
      541 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct
      601 tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca
      661 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
      721 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
      781 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
      841 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
      901 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
      961 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg
     1021 aaggcgagag tagggaactg ccaggcatca aactaagcag aaggcccctg acggatggcc
     1081 tttttgcgtt tctacaaact ctttctgtgt tgtaaaacga cggccagtct taagctcggg
     1141 ccccctgggc ggttctgata acgagtaatc gttaatccgc aaataacgta aaaacccgct
     1201 tcggcgggtt tttttatggg gggagtttag ggaaagagca tttgtcagaa tatttaaggg
     1261 cgcctgtcac tttgcttgat atatgagaat tatttaacct tataaatgag aaaaaagcaa
     1321 cgcactttaa ataagatacg ttgctttttc gattgatgaa cacctataat taaactattc
     1381 atctattatt tatgattttt tgtatataca atatttctag tttgttaaag agaattaaga
     1441 aaataaatct cgaaaataat aaagggaaaa tcagtttttg atatcaaaat tatacatgtc
     1501 aacgataata caaaatataa tacaaactat aagatgttat cagtatttat tatcatttag
     1561 aataaatttt gtgtcgccct taattgtgag cggataacaa ttacgagctt catgcacagt
     1621 ggcgttgact caatattggc cattagccat attattcatt ggttatatag cataaatcaa
     1681 tattggctat tggccattgc atacgttgta tctatatcat aatatgtaca tttatattgg
     1741 ctcatgtcca atatgaccgc catgttggca ttgattattg actagttatt aatagtaatc
     1801 aattacgggg tcattagttc atagcccata tatggagttc cgcgttacat aacttacggt
     1861 aaatggcccg cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta
     1921 tgttcccata gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg
     1981 gtaaactgcc cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga
     2041 cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag tacatgacct tatgggactt
     2101 tcctacttgg cagtacatct acgtattagt catcgctatt accatggtga tgcggttttg
     2161 gcagtacatc aatgggcgtg gatagcggtt tgactcacgg ggatttccaa gtctccaccc
     2221 cattgacgtc aatgggagtt tgttttggca ccaaaatcaa cgggactttc caaaatgtcg
     2281 taacaactcc gccccattga cgcaaatggg cggtaggcgt gtacggtggg aggtctatat
     2341 aagcagagct cgtttagtga accgtcagat cgcctggaga cgccatccac gctgttttga
     2401 cctccataga agacaccggg accgatccag cctccgcggc cgggaacggt gcattggaac
     2461 gcggattccc cgtgccaaga gtgacgtaag taccgcctat agagtctata ggcccacccc
     2521 cttggcttcg ttagaacgcg gctacaatta atacataacc ttatgtatca tacacatacg
     2581 atttaggtga cactatagaa taacatccac tttgcctttc tctccacagg tgtccactcc
     2641 caggtccaac tgcacctcgg ttctatcgaa aacgcgcctg ctagcgccac catgaagacc
     2701 atcattgccc tgagctacat cttctgcctg gtgttcgctg cggccgccga agatctgcac
     2761 agcagcctgt acttcgtgaa cgccagcctg caagaggtgg tgttcgccag cacaacaggc
     2821 accctggttc cttgtcctgc cgccggaatt cctcctgtga cactgagatg gtatctggcc
     2881 accggcgaag agatctacga tgtgcctggc atcagacacg tgcaccccaa tggaaccctg
     2941 cagattttcc catttcctcc aagcagcttc agcaccctga tccacgacaa cacctactac
     3001 tgcaccgccg agaatcccag cggcaagatc agatcccagg acgtgcacat caaggccgtg
     3061 ctgagagaac cctacaccgt gcgcgtggaa gatcagaaaa ccatgagagg caacgtggcc
     3121 gtgttcaagt gcatcatccc cagcagcgtg gaagcctaca tcaccgtggt gtcctgggag
     3181 aaagacaccg tgtctctggt gtccggcagc cggtttctga tcacatctac tggcgccctg
     3241 tacatcaagg atgtgcagaa cgaggacggc ctgtacaact accggtgcat cacccggcac
     3301 cggtacacag gcgaaaccag acagagcaac agcgccagac tgttcgtgtc tgaccccgcc
     3361 aatagcgccc cttctatcct ggatggcttc gaccacagaa aggccatggc tggccagaga
     3421 gtggaactgc cttgtaaagc cctgggacac cccgagcctg actacagatg gctgaaggac
     3481 aacatgcccc tggaactgag cggccggttc cagaaaacag tgaccggact gctgatcgag
     3541 aacatcagac ccagcgacag cggcagctac gtgtgcgagg tgtccaatag atacggcacc
     3601 gccaaagtga tcggccggct gtatgtgaag cagcccctga aggccacaat cagccccaga
     3661 aaagtgaagt ccagcgtggg ctctcaggtg tccctgagct gttctgtgac aggcaccgag
     3721 gaccaagagc tgagctggta cagaaacggc gagattctga accccggcaa gaacgtgcgg
     3781 atcaccggca tcaaccacga gaacctgatc atggaccaca tggtcaagag cgacggcgga
     3841 gcctaccagt gctttgtgcg gaaggataag ctgagcgccc aggactatgt gcaggtcgtg
     3901 ctggaagatg gcacccctaa gatcatcagc gccttcagcg agaaggtggt gtctcctgcc
     3961 gaacctgtgt ctctgatgtg caacgtgaag ggcacccctc tgcctaccat cacctggaca
     4021 ctggacgacg accccatcct gaaaggcggc agccacagaa tcagccagat gatcaccagc
     4081 gagggcaatg tggtgtctta cctgaacatc agcagcagcc aagtccgaga tggcggcgtg
     4141 tacagatgta ccgccaacaa ttctgccggc gtggtgctgt accaggccag aatcaatgtt
     4201 cggggccctg ccagcatcag gcccatgaag aatatcaccg ccattgccgg cagagatacc
     4261 tacatccact gccgcgtgat cggctacccc tactacagca tcaagtggta caagaacagc
     4321 aacctgctgc cgttcaacca cagacaggtg gccttcgaga acaatggcac cctgaagctg
     4381 tccgacgtgc agaaagaagt ggacgagggc gagtacacct gtaacgtgct ggttcagcca
     4441 cagctgagca ccagccagtc tgtgcacgtg acagtgaagg tgccaccttt catccagcct
     4501 ttcgagttcc ccagattcag catcggccag cgggtgttca tcccttgtgt ggtcgttagc
     4561 ggcgacctgc ctatcaccat cacatggcag aaggacggca gacccattcc tggatctctg
     4621 ggcgtgacca tcgacaacat cgacttcacc tccagcctgc ggatctccaa cctgagcctg
     4681 atgcacaacg gcaactacac atgtatcgcc cggaatgagg ccgctgccgt ggaacatcag
     4741 tctcagctga tcgtcagagt gcctcctaag ttcgtggtgc agcctagaga tcaggacggc
     4801 atctacggaa aggccgtgat cctgaattgc agcgccgagg gctatcccgt gcctacaatc
     4861 gtgtggaagt tcagcaaagg cgctggcgtg ccccagttcc agcctatcgc tctgaatggc
     4921 agaatccagg tgctgagcaa cggcagcctg ctgatcaagc acgtggtgga agaggacagc
     4981 ggctactacc tgtgtaaagt gtccaacgac gtgggcgccg acgtgtccaa gagcatgtat
     5041 ctgaccgtga agatccccgc catgatcaca agctacccca acaccacact ggccacacag
     5101 ggccagaaaa aagagatgag ctgcacagcc cacggcgaga agcccatcat cgtcagatgg
     5161 gaaaaagagg accggatcat caaccctgag atggcccgct acctggtgtc caccaaagaa
     5221 gtcggcgagg aagtcatcag cacactgcag atcctgccta ccgtgcggga agatagcggc
     5281 ttctttagct gccacgccat caacagctac ggcgaggaca gaggcatcat ccagctgaca
     5341 gtgcaagagc ctcctgatcc tcctgagatc gagatcaagg acgtgaaggc ccggaccatc
     5401 acactgaggt ggaccatggg cttcgacggc aacagcccta tcaccggcta cgacatcgag
     5461 tgcaagaaca agtccgacag ctgggacagc gcccagagga caaaggatgt gtcccctcag
     5521 ctgaactccg ccaccatcat cgatattcac cccagctcca cctactccat caggatgtac
     5581 gccaagaacc ggatcggcaa gagcgagccc agcaacgagc tgacaatcac cgctgatgag
     5641 gctgcccctg atggccctcc tcaagaagtg cacctggaac ctatcagcag ccagagcatc
     5701 agagtgacct ggaaggcccc taagaagcac ctccagaacg gcatcatcag aggctaccag
     5761 atcggctata gagagtactc caccggcggc aacttccagt tcaacatcat ctccgtggat
     5821 accagcggcg acagcgaggt gtacacactg gacaacctga acaagttcac ccagtacggc
     5881 ctggtggtgc aggcctgtaa tagagccgga acaggccctt ccagccaaga gattatcacc
     5941 accacactcg aggacgtgcc cagctatcct ccagagaacg tgcaggccat tgccacctct
     6001 ccagagagca tctccatcag ctggtctacc ctgagcaaag aggccctgaa cggaatcctg
     6061 cagggcttca gagtgatcta ctgggccaac ctgatggacg gcgagctggg agagatcaag
     6121 aacattacca ccactcagcc cagcctcgag ctggatggcc tggaaaagta caccaattac
     6181 agcattcagg tgctggcctt caccagagct ggcgacggcg ttagaagcga gcagatcttt
     6241 acccggacca aagaggacgt ccccggacct cctgctggtg tcaaagctgc agcagcctct
     6301 gcctctatgg tgtttgtgtc ctggctgcct cctctgaagc tgaacgggat catccggaag
     6361 tacaccgtgt tttgctctca cccctatcct accgtgatca gcgagttcga ggcctctcct
     6421 gacagcttct cctacagaat ccccaacctg tccagaaaca gacagtacag cgtgtgggtc
     6481 gtcgccgtga catctgccgg aagaggcaat agcagcgaga tcatcacagt ggaacccctg
     6541 gccaaggctc ccgccagaat cctgacattt tccggcaccg tgaccacacc ttggatgaag
     6601 gacatcgtgc tgccctgcaa agccgtgggc gatccatctc ctgctgtgaa gtggatgaag
     6661 gatagcaacg gcacccctag cctggtcaca atcgacggca gaagatccat cttcagcaat
     6721 ggcagcttca tcatcaggac cgtgaaagcc gaggactccg gctactatag ctgcattgcc
     6781 aacaacaact ggggctccga tgagatcatc ctgaacctgc aagtgcaggt cccacctgac
     6841 cagcctagac tgaccgtgtc taagaccacc agctccagca tcaccctgtc ttggctgcca
     6901 ggcgataatg gcggcagctc tatcaggggc tacatcctgc agtactccga ggacaactct
     6961 gagcagtggg gcagcttccc tatcagccct agcgagagaa gctaccggct cgagaatctg
     7021 aagtgcggca catggtacaa gtttaccctg acagcccaga atggcgtcgg cccaggcaga
     7081 atctccgaga tcattgaggc caagacactg ggcaaagagc ctcagttctc caaagagcaa
     7141 gagctgttcg cctccatcaa caccaccaga gtgcgcctga atctgatcgg ctggaatgac
     7201 ggcggctgtc ccatcaccag ctttaccctg gaataccggc ctttcggcac caccgtgtgg
     7261 acaactgccc agagaacaag cctgagcaag tcttacatcc tgtacgacct ccaagaggcc
     7321 acttggtacg agctgcagat gagagtgtgt aacagcgccg gctgtgccga gaagcaggcc
     7381 aatttcgcca cactgaacta cgacggcagc acaatccctc cactgatcaa gtctgtggtg
     7441 cagaatgaag agggcctgac cacaaacgag ggactgaaga tgggcgcgcc ggaagatcaa
     7501 gttgatccaa gattgattga tggcaagggc ggatctctgg aggtgctgtt ccagggacca
     7561 ggcggaagcg gcaatcccgg cggcgatctg gccccccaga tgctgcgcga gctgcaggag
     7621 accaatgccg ccctgcagga cgtgcgcgag ctgctgcgcc accgcgtgaa ggagatcacc
     7681 ttcctgaaga acaccgtgat ggagtgcgac gcctgcggta tgcagccctc tagaggctcc
     7741 ggctccatca tcccagttga ggaggagaac ccggacttct ggaaccgcga ggcagccgag
     7801 gccctgggtg ccgccaagaa gctgcagcct gcacagacag ccgccaagaa cctcatcatc
     7861 ttcctgggcg atgggatggg ggtgtctacg gtgacagctg ccaggatcct aaaagggcag
     7921 aagaaggaca aactggggcc tgagataccc ctggccatgg accgcttccc atatgtggct
     7981 ctgtccaaga catacaatgt agacaaacat gtgccagaca gtggagccac agccacggcc
     8041 tacctgtgcg gggtcaaggg caacttccag accattggct tgagtgcagc cgcccgcttt
     8101 aaccagtgca acacgacacg cggcaacgag gtcatctccg tgatgaatcg ggccaagaaa
     8161 gcagggaagt cagtgggagt ggtaaccacc acacgagtgc agcacgcctc gccagccggc
     8221 acctacgccc acacggtgaa ccgcaactgg tactcggacg ccgacgtgcc tgcctcggcc
     8281 cgccaggagg ggtgccagga catcgctacg cagctcatct ccaacatgga cattgacgtg
     8341 atcctaggtg gaggccgaaa gtacatgttt cgcatgggaa ccccagaccc tgagtaccca
     8401 gatgactaca gccaaggtgg gaccaggctg gacgggaaga atctggtgca ggaatggctg
     8461 gcgaagcgcc agggtgcccg gtatgtgtgg aaccgcactg agctcatgca ggcttccctg
     8521 gacccgtctg tgacccatct catgggtctc tttgagcctg gagacatgaa atacgagatc
     8581 caccgagact ccacactgga cccctccctg atggagatga cagaggctgc cctgcgcctg
     8641 ctgagcagga acccccgcgg cttcttcctc ttcgtggagg gtggtcgcat cgaccatggt
     8701 catcatgaaa gcagggctta ccgggcactg actgagacga tcatgttcga cgacgccatt
     8761 gagagggcgg gccagctcac cagcgaggag gacacgctga gcctcgtcac tgccgaccac
     8821 tcccacgtct tctccttcgg aggctacccc ctgcgaggga gctccatctt cgggctggcc
     8881 cctggcaagg cccgggacag gaaggcctac acggtcctcc tatacggaaa cggtccaggc
     8941 tatgtgctca aggacggcgc ccggccggat gttaccgaga gcgagagcgg gagccccgag
     9001 tatcggcagc agtcagcagt gcccctggac gaagagaccc acgcaggcga ggacgtggcg
     9061 gtgttcgcgc gcggcccgca ggcgcacctg gttcacggcg tgcaggagca gaccttcata
     9121 gcgcacgtca tggccttcgc cgcctgcctg gagccctaca ccgcctgcga cctggcgccc
     9181 cccgccggca ccaccgacgc cgcgcacccg ggtggcggag gactgaacga catcttcgag
     9241 gctcagaaaa tcgaatggca cgaacatcac catcaccatc accatcacca ttaaggccgg
     9301 cctgtagtca ctggcacaac gcgttacccg gtaagccaat cgggtataca cggtcgtcat
     9361 actgcagaca gggttcttct actttgcaag atagtcttga gtagtaaaat aaatagatag
     9421 agaaaatcag cctcgactgt gccttctagt tgccagccat ctgttgtttg cccctccccc
     9481 gtgccttcct tgaccctgga aggtgccact cccactgtcc tttcctaata aaatgaggaa
     9541 attgcatcac aacactcaac cctatctcgg tctattcttt tgatttataa gggattttgc
     9601 cgatttcggc ctattggtta aaaaatgagc tgatttaaca aaaatttaac gcgaattaat
     9661 tctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag caggcagaag
     9721 tatgcaaagc atgcatctca attagtcagc aaccaggtgt ggaaagtccc caggctcccc
     9781 agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccatag tcccgcccct
     9841 aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg
     9901 actaattttt tttatttatg cagaggccga ggccgcctct gcctctgagc tattccagaa
     9961 gtagtgagga ggcttttttg gaggcctagg cttttgcaaa aagctcccgg gagcttgtat
    10021 atccattttc ggatctgatc agcacgtgtt gacaattaat catcggcata gtatatcggc
    10081 atagtataat acgacaaggt gaggaactaa accatgaccg agtacaagcc cacggtgcgc
    10141 ctcgccaccc gcgacgacgt ccccagggcc gtacgcaccc tcgccgccgc gttcgccgac
    10201 taccccgcca cgcgccacac cgtcgatccg gaccgccaca tcgagcgggt caccgagctg
    10261 caagaactct tcctcacgcg cgtcgggctc gacatcggca aggtgtgggt cgcggacgac
    10321 ggcgccgcgg tggcggtctg gaccacgccg gagagcgtcg aagcgggggc ggtgttcgcc
    10381 gagatcggcc cgcgcatggc cgagttgagc ggttcccggc tggccgcgca gcaacagatg
    10441 gaaggcctcc tggcgccgca ccggcccaag gagcccgcgt ggttcctggc caccgtcggc
    10501 gtctcgcccg accaccaggg caagggtctg ggcagcgccg tcgtgctccc cggagtggag
    10561 gcggccgagc gcgccggggt gcccgccttc ctggagacat ccgcgccccg caacctcccc
    10621 ttctacgagc ggctcggctt caccgtcacc gccgacgtcg aggtgcccga aggaccgcgc
    10681 acctggtgca tgacccgcaa gcccggtgcc tgacacgtgc tacgagattt cgattccacc
    10741 gccgccttct atgaaaggtt gggcttcgga atcgttttcc gggacgccgg ctggatgatc
    10801 ctccagcgcg gggatctcat gctggagttc ttcgcccacc ccaacttgtt tattgcagct
    10861 tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc atttttttca
    10921 ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctgtataccg
    10981 tcgacctcta gctagagctt ggcgtaatca tggtcattac caatgcttaa tcagtgaggc
    11041 acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta
    11101 gataactacg atacgggagg gcttaccatc tggccccagc gctgcgatga taccgcgaga
    11161 accacgctca ccggctccgg atttatcagc aataaaccag ccagccggaa gggccgagcg
    11221 cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc
    11281 tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccatcg ctacaggcat
    11341 cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag
    11401 gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat
    11461 cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa
    11521 ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa
    11581 gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga
    11641 taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg
    11701 gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc
    11761 acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg
    11821 aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatatt
    11881 cttcctt
//
