LOCUS       sequence-304327-       11965 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of cell-surface protein partial extracellular 
            domain fused to COMP5AP-AviTag-9xHis. Protein is secreted from 
            cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11965)
  AUTHORS   Wojtowicz WM, Vielmetter J, Fernandes RA, Siepe DH, Eastman CL, 
            Chisholm GB, Cox S, Klock H, Anderson PW, Rue SM, Miller JJ, Glaser 
            SM, Bragstad ML, Vance J, Lam AW, Lesley SA, Zinn K, Garcia KC
  TITLE     A Human IgSF Cell-Surface Interactome Reveals a Complex Network of 
            Protein-Protein Interactions.
  JOURNAL   Cell. 2020 Aug 20;182(4):1027-1043.e17. doi: 
            10.1016/j.cell.2020.07.025.
  PUBMED    32822567
REFERENCE   2  (bases 1 to 11965)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2020.07"; journalName: "Cell"; date: "2020-08-20- 
            20"; volume: "182"; issue: "4"; pages: "1027-1043.e17"
COMMENT     Sequence Label: pD649-HAsp-NOTCH3(Trunc1)-COMP5AP-AviTag-9xHis
FEATURES             Location/Qualifiers
     source          1..11965
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     124..143
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     149..168
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     rep_origin      254..842
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     743..762
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     996..1013
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     1111..1128
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     1112..1128
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    1584..1600
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     enhancer        1767..2146
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2147..2350
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2301..2321
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     2347..2371
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        2581..2599
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     2581..2598
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     2612..2631
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     regulatory      4292..4301
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      5547..5556
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             7570..7605
                     /codon_start=1
                     /product="epitope tag derived from human Protein C (Stearns
                     et al., 1988)"
                     /label=Protein C tag
                     /translation="EDQVDPRLIDGK"
     CDS             7615..7638
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     CDS             9298..9342
                     /codon_start=1
                     /product="peptide tag that allows for enzymatic 
                     biotinylation"
                     /label=AviTag(TM)
                     /translation="GLNDIFEAQKIEWHE"
     CDS             9343..9369
                     /codon_start=1
                     /product="9xHis affinity tag"
                     /label=9xHis
                     /translation="HHHHHHHHH"
     primer_bind     complement(9509..9526)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    9515..9626
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(9744..9764)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        9749..10078
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      9929..10064
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     9991..10010
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        10126..10173
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             10192..10791
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(10192..10211)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     10688..10708
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     polyA_signal    10921..11042
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10958..10977)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     11012..11031
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     CDS             complement(join(11095..11886,11887..11955))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11718..11737
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(join(11956..11965,1..82))
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa
       61 tgtatttaga aaaataaaca aataggggtc agtgttacaa ccaattaacc aattctgaac
      121 attatcgcga gcccatttat acctgaatat ggctcataac accccttgct catgaccaaa
      181 atcccttaac gtgagttacg cgcgcgtcgt tccactgagc gtcagacccc gtagaaaaga
      241 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa
      301 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga
      361 aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt
      421 tagcccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt
      481 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat
      541 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct
      601 tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca
      661 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
      721 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
      781 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
      841 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
      901 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
      961 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg
     1021 aaggcgagag tagggaactg ccaggcatca aactaagcag aaggcccctg acggatggcc
     1081 tttttgcgtt tctacaaact ctttctgtgt tgtaaaacga cggccagtct taagctcggg
     1141 ccccctgggc ggttctgata acgagtaatc gttaatccgc aaataacgta aaaacccgct
     1201 tcggcgggtt tttttatggg gggagtttag ggaaagagca tttgtcagaa tatttaaggg
     1261 cgcctgtcac tttgcttgat atatgagaat tatttaacct tataaatgag aaaaaagcaa
     1321 cgcactttaa ataagatacg ttgctttttc gattgatgaa cacctataat taaactattc
     1381 atctattatt tatgattttt tgtatataca atatttctag tttgttaaag agaattaaga
     1441 aaataaatct cgaaaataat aaagggaaaa tcagtttttg atatcaaaat tatacatgtc
     1501 aacgataata caaaatataa tacaaactat aagatgttat cagtatttat tatcatttag
     1561 aataaatttt gtgtcgccct taattgtgag cggataacaa ttacgagctt catgcacagt
     1621 ggcgttgact caatattggc cattagccat attattcatt ggttatatag cataaatcaa
     1681 tattggctat tggccattgc atacgttgta tctatatcat aatatgtaca tttatattgg
     1741 ctcatgtcca atatgaccgc catgttggca ttgattattg actagttatt aatagtaatc
     1801 aattacgggg tcattagttc atagcccata tatggagttc cgcgttacat aacttacggt
     1861 aaatggcccg cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta
     1921 tgttcccata gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg
     1981 gtaaactgcc cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga
     2041 cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag tacatgacct tatgggactt
     2101 tcctacttgg cagtacatct acgtattagt catcgctatt accatggtga tgcggttttg
     2161 gcagtacatc aatgggcgtg gatagcggtt tgactcacgg ggatttccaa gtctccaccc
     2221 cattgacgtc aatgggagtt tgttttggca ccaaaatcaa cgggactttc caaaatgtcg
     2281 taacaactcc gccccattga cgcaaatggg cggtaggcgt gtacggtggg aggtctatat
     2341 aagcagagct cgtttagtga accgtcagat cgcctggaga cgccatccac gctgttttga
     2401 cctccataga agacaccggg accgatccag cctccgcggc cgggaacggt gcattggaac
     2461 gcggattccc cgtgccaaga gtgacgtaag taccgcctat agagtctata ggcccacccc
     2521 cttggcttcg ttagaacgcg gctacaatta atacataacc ttatgtatca tacacatacg
     2581 atttaggtga cactatagaa taacatccac tttgcctttc tctccacagg tgtccactcc
     2641 caggtccaac tgcacctcgg ttctatcgaa aacgcgcctg ctagcgccac catgaagacc
     2701 atcattgccc tgagctacat cttctgcctg gtgttcgctg cggccgccgc tcctccttgt
     2761 cttgatggat ctccttgtgc caacggcggc agatgtacac agctgcctag cagagaagcc
     2821 gcctgtcttt gtcctcctgg ctgggttgga gaacggtgcc agctggaaga tccttgtcac
     2881 tctggacctt gtgccggcag aggcgtgtgt caatcttctg tggtggccgg cacagcccgg
     2941 ttcagctgta gatgtcctag aggcttcaga ggccccgact gtagcctgcc tgatccatgt
     3001 ctcagcagcc cttgtgctca tggcgccaga tgttctgtgg gccctgatgg cagattcctg
     3061 tgtagctgtc ctccaggcta ccagggcaga tcctgcagat ccgatgtgga tgagtgtaga
     3121 gtgggcgagc cttgtagaca cggcggcact tgtctgaata cccctggcag cttcagatgc
     3181 cagtgtcctg ccggatatac aggccctctg tgcgaaaatc ctgccgtgcc atgtgcaccc
     3241 tctccatgca gaaatggcgg cacctgtaga cagagcggcg acctgacata cgattgtgcc
     3301 tgtctgcctg gcttcgaggg ccagaactgc gaagtgaatg tggacgattg ccctggccac
     3361 agatgcctga atggcggaac atgtgtggac ggcgtgaaca cctacaactg tcagtgtcca
     3421 cctgagtgga ccggccagtt ctgtactgag gatgtcgacg agtgtcagct gcagcccaat
     3481 gcctgtcata acggcggaac ctgctttaac acactcggcg gccatagctg cgtgtgtgtg
     3541 aatggatgga caggcgagag ctgctcccag aacatcgatg attgtgccac cgccgtgtgc
     3601 tttcacggcg ccacatgtca cgatagagtg gccagctttt actgcgcctg tcctatgggc
     3661 aagaccggcc tgctgtgtca cctggatgac gcctgtgtgt ccaatccttg ccacgaggac
     3721 gccatctgcg acacaaaccc tgtgaacggc agagccatct gtacatgccc acctggcttt
     3781 accggcggag cctgtgatca ggacgtggac gagtgttcta tcggcgccaa tccatgcgag
     3841 cacctgggca gatgtgtgaa cacacagggc agctttctgt gtcagtgcgg cagaggatac
     3901 accggaccta gatgcgagac agatgtgaac gagtgcctga gcggcccctg cagaaatcag
     3961 gctacctgtc tggaccggat cggccagttt acctgtatct gcatggccgg cttcaccggc
     4021 acctattgcg aggtggacat tgatgagtgc cagagcagtc cttgcgtgaa cggcggagtg
     4081 tgcaaggaca gagtgaatgg cttcagctgc acatgcccaa gcggcttttc tggcagcacc
     4141 tgtcagctgg acgtcgacga atgtgccagc acaccatgta gaaacggcgc taagtgcgtg
     4201 gaccagcctg atggctacga gtgtagatgt gccgagggct ttgagggcac cctgtgcgac
     4261 agaaatgtcg atgactgcag ccccgatcca tgccaccatg gtagatgtgt ggatggaatc
     4321 gccagcttct cctgtgcttg tgcccctggc tatacaggca ccagatgcga atcccaggtg
     4381 gacgaatgca gaagccagcc ttgcaggcat ggcggcaaat gcctggacct ggtggacaag
     4441 tacctgtgta gatgcccttc tggcaccaca ggcgtgaact gtgaagtcaa catcgacgac
     4501 tgcgcctcta atccctgcac cttcggcgtg tgcagagatg gcatcaacag atacgactgc
     4561 gtgtgccagc caggcttcac aggacccctg tgcaacgtgg aaatcaacga gtgcgcttct
     4621 agcccatgtg gcgaaggcgg atcttgtgtc gatggcgaga acggctttcg gtgtctgtgc
     4681 cctcctggat ctctgcctcc tctgtgtctg cctccatctc acccatgtgc tcacgagcca
     4741 tgcagccacg gcatctgtta tgatgcccca ggcggattca gatgcgtgtg cgaacctgga
     4801 tggtccgggc ctagatgttc tcagtctctg gctagagatg cctgcgagtc tcagccatgt
     4861 agagctggcg gcacatgcag ctctgatggc atgggctttc actgcacgtg ccctccaggt
     4921 gttcagggca gacaatgcga gctgctgagc ccctgtacac ctaatccatg tgaacacggc
     4981 ggaagatgcg agtctgctcc tggacaactg ccagtctgtt cttgccctca aggatggcag
     5041 ggaccaagat gccagcagga tgttgatgaa tgcgctggcc ctgctccttg tggccctcac
     5101 ggaatctgta caaatctggc cggctccttt agctgtacct gccacggtgg ctataccgga
     5161 ccaagctgcg accaggacat caacgattgc gacccaaatc catgcctcaa cggcgggtct
     5221 tgtcaggatg gcgtgggcag cttctcttgt agctgcctgc ctggattcgc tggccccaga
     5281 tgtgcccgag atgtcgatga gtgcctgtct aatccttgcg gccctggcac ctgtacagac
     5341 cacgtggcaa gcttcacatg cacctgtccg cctggatacg gcggatttca ctgtgaacag
     5401 gacctgccag attgcagccc cagcagctgt tttaatggcg ggacttgcgt cgacggggtc
     5461 aacagctttt cctgtctgtg cagaccaggc tacactggcg cccactgtca gcatgaagct
     5521 gacccttgtc tgagcaggcc atgtctccat ggcggcgtgt gttctgccgc tcatccaggc
     5581 tttagatgca cttgcctgga atccttcaca ggcccacagt gccagacact ggtggattgg
     5641 tgtagcagac agccctgcca aaatggtggc agatgcgttc agacaggcgc ctactgtttg
     5701 tgtcctcctg gatggtctgg ccggctgtgc gatatcagaa gcctgccttg tagagaggcc
     5761 gctgctcaga tcggcgttag actggaacaa ctgtgtcagg ctggcggcca gtgtgttgat
     5821 gaggatagca gccactactg tgtgtgccct gagggaagaa ccggctctca ctgcgaacaa
     5881 gaggtggacc catgtctggc ccagccgtgt cagcacggtg gaacatgtag aggctacatg
     5941 ggcggctaca tgtgcgagtg tctgcccggc tacaacggcg acaattgcga ggatgatgtt
     6001 gacgagtgcg ccagccaacc ttgtcaacat ggcggctctt gcatcgatct ggtggccaga
     6061 tacctgtgca gttgcccacc aggcacactc ggagtgctgt gcgagattaa cgaggacgat
     6121 tgtggccccg gacctcctct ggatagtggc cctagatgtc tgcacaacgg gacctgtgtg
     6181 gatctcgtcg gaggcttccg atgcacatgt ccaccgggct atactggact gagatgcgag
     6241 gccgatatca atgagtgcag aagtggcgct tgtcacgccg ctcacaccag agattgtctg
     6301 caagatcccg gcggaggatt ccgctgtctc tgtcatgctg gattcagcgg ccctaggtgt
     6361 cagacagtgc tgtccccttg tgaaagccag ccatgccaac acggtggcca gtgcagacct
     6421 tctccaggac ctggcggagg cctgaccttt acatgtcact gtgcccagcc tttctggggc
     6481 ccaagatgcg aaagagtggc ccggtcctgt agagaactcc agtgtccagt gggagtgcct
     6541 tgccagcaaa cacctagagg acccagatgt gcttgccctc ctggcctctc tggacctagc
     6601 tgtagaagct ttccaggatc tccaccaggc gccagcaatg cctcttgtgc tgctgctcca
     6661 tgccttcatg gtggaagctg tagacctgct cctctggcac ccttcttcag atgtgcatgc
     6721 gcccaaggct ggactgggcc aagatgtgaa gcaccagctg ctgcccctga ggtgtccgaa
     6781 gaacctcgat gtccaagagc tgcctgtcag gccaaaaggg gcgaccagag atgcgacaga
     6841 gagtgcaatt ctcccggctg tggatgggat ggcggcgatt gcagtctgtc tgtgggagat
     6901 ccttggagac agtgcgaagc cctgcagtgc tggcggctgt tcaacaacag cagatgcgat
     6961 cctgcctgca gctccccagc ctgtctgtac gacaatttcg attgccacgc aggcggcaga
     7021 gagcggacct gtaatcctgt gtacgagaag tactgcgccg accacttcgc cgacggaaga
     7081 tgtgaccagg gctgcaacac cgaggaatgc ggctgggatg gactggattg tgcttccgaa
     7141 gttcccgctc tgcttgctag aggcgtgctg gttctgacag ttctgctgcc accagaggaa
     7201 ctgctgagaa gcagcgccga tttcctgcag agactgtccg ctatcctgag aaccagcctg
     7261 cggttcagac tggatgctca cggacaggcc atggtgttcc cttaccatag gccatctcca
     7321 ggcagcgagc ctagagccag aagagaactg gctcctgaag tgatcggcag cgtcgtgatg
     7381 ctggaaatcg acaacagact gtgcctgcag agccccgaga acgaccactg tttccctgat
     7441 gctcagagcg ccgctgatta tctgggagcc ctgtctgctg tggaacggct ggactttccc
     7501 tatcctctga gagatgtgcg gggcgagcca ctggaacctc cagaaccttc tgtgcccctt
     7561 ggcgcgccgg aagatcaagt tgatccaaga ttgattgatg gcaagggcgg atctctggag
     7621 gtgctgttcc agggaccagg cggaagcggc aatcccggcg gcgatctggc cccccagatg
     7681 ctgcgcgagc tgcaggagac caatgccgcc ctgcaggacg tgcgcgagct gctgcgccac
     7741 cgcgtgaagg agatcacctt cctgaagaac accgtgatgg agtgcgacgc ctgcggtatg
     7801 cagccctcta gaggctccgg ctccatcatc ccagttgagg aggagaaccc ggacttctgg
     7861 aaccgcgagg cagccgaggc cctgggtgcc gccaagaagc tgcagcctgc acagacagcc
     7921 gccaagaacc tcatcatctt cctgggcgat gggatggggg tgtctacggt gacagctgcc
     7981 aggatcctaa aagggcagaa gaaggacaaa ctggggcctg agatacccct ggccatggac
     8041 cgcttcccat atgtggctct gtccaagaca tacaatgtag acaaacatgt gccagacagt
     8101 ggagccacag ccacggccta cctgtgcggg gtcaagggca acttccagac cattggcttg
     8161 agtgcagccg cccgctttaa ccagtgcaac acgacacgcg gcaacgaggt catctccgtg
     8221 atgaatcggg ccaagaaagc agggaagtca gtgggagtgg taaccaccac acgagtgcag
     8281 cacgcctcgc cagccggcac ctacgcccac acggtgaacc gcaactggta ctcggacgcc
     8341 gacgtgcctg cctcggcccg ccaggagggg tgccaggaca tcgctacgca gctcatctcc
     8401 aacatggaca ttgacgtgat cctaggtgga ggccgaaagt acatgtttcg catgggaacc
     8461 ccagaccctg agtacccaga tgactacagc caaggtggga ccaggctgga cgggaagaat
     8521 ctggtgcagg aatggctggc gaagcgccag ggtgcccggt atgtgtggaa ccgcactgag
     8581 ctcatgcagg cttccctgga cccgtctgtg acccatctca tgggtctctt tgagcctgga
     8641 gacatgaaat acgagatcca ccgagactcc acactggacc cctccctgat ggagatgaca
     8701 gaggctgccc tgcgcctgct gagcaggaac ccccgcggct tcttcctctt cgtggagggt
     8761 ggtcgcatcg accatggtca tcatgaaagc agggcttacc gggcactgac tgagacgatc
     8821 atgttcgacg acgccattga gagggcgggc cagctcacca gcgaggagga cacgctgagc
     8881 ctcgtcactg ccgaccactc ccacgtcttc tccttcggag gctaccccct gcgagggagc
     8941 tccatcttcg ggctggcccc tggcaaggcc cgggacagga aggcctacac ggtcctccta
     9001 tacggaaacg gtccaggcta tgtgctcaag gacggcgccc ggccggatgt taccgagagc
     9061 gagagcggga gccccgagta tcggcagcag tcagcagtgc ccctggacga agagacccac
     9121 gcaggcgagg acgtggcggt gttcgcgcgc ggcccgcagg cgcacctggt tcacggcgtg
     9181 caggagcaga ccttcatagc gcacgtcatg gccttcgccg cctgcctgga gccctacacc
     9241 gcctgcgacc tggcgccccc cgccggcacc accgacgccg cgcacccggg tggcggagga
     9301 ctgaacgaca tcttcgaggc tcagaaaatc gaatggcacg aacatcacca tcaccatcac
     9361 catcaccatt aaggccggcc tgtagtcact ggcacaacgc gttacccggt aagccaatcg
     9421 ggtatacacg gtcgtcatac tgcagacagg gttcttctac tttgcaagat agtcttgagt
     9481 agtaaaataa atagatagag aaaatcagcc tcgactgtgc cttctagttg ccagccatct
     9541 gttgtttgcc cctcccccgt gccttccttg accctggaag gtgccactcc cactgtcctt
     9601 tcctaataaa atgaggaaat tgcatcacaa cactcaaccc tatctcggtc tattcttttg
     9661 atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg atttaacaaa
     9721 aatttaacgc gaattaattc tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg
     9781 ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg
     9841 aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc
     9901 aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca
     9961 ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg ccgcctctgc
    10021 ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct tttgcaaaaa
    10081 gctcccggga gcttgtatat ccattttcgg atctgatcag cacgtgttga caattaatca
    10141 tcggcatagt atatcggcat agtataatac gacaaggtga ggaactaaac catgaccgag
    10201 tacaagccca cggtgcgcct cgccacccgc gacgacgtcc ccagggccgt acgcaccctc
    10261 gccgccgcgt tcgccgacta ccccgccacg cgccacaccg tcgatccgga ccgccacatc
    10321 gagcgggtca ccgagctgca agaactcttc ctcacgcgcg tcgggctcga catcggcaag
    10381 gtgtgggtcg cggacgacgg cgccgcggtg gcggtctgga ccacgccgga gagcgtcgaa
    10441 gcgggggcgg tgttcgccga gatcggcccg cgcatggccg agttgagcgg ttcccggctg
    10501 gccgcgcagc aacagatgga aggcctcctg gcgccgcacc ggcccaagga gcccgcgtgg
    10561 ttcctggcca ccgtcggcgt ctcgcccgac caccagggca agggtctggg cagcgccgtc
    10621 gtgctccccg gagtggaggc ggccgagcgc gccggggtgc ccgccttcct ggagacatcc
    10681 gcgccccgca acctcccctt ctacgagcgg ctcggcttca ccgtcaccgc cgacgtcgag
    10741 gtgcccgaag gaccgcgcac ctggtgcatg acccgcaagc ccggtgcctg acacgtgcta
    10801 cgagatttcg attccaccgc cgccttctat gaaaggttgg gcttcggaat cgttttccgg
    10861 gacgccggct ggatgatcct ccagcgcggg gatctcatgc tggagttctt cgcccacccc
    10921 aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca
    10981 aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct
    11041 tatcatgtct gtataccgtc gacctctagc tagagcttgg cgtaatcatg gtcattacca
    11101 atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc
    11161 ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagcgc
    11221 tgcgatgata ccgcgagaac cacgctcacc ggctccggat ttatcagcaa taaaccagcc
    11281 agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat
    11341 taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt
    11401 tgccatcgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc
    11461 cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag
    11521 ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt
    11581 tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac
    11641 tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg
    11701 cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat
    11761 tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc
    11821 gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc
    11881 tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa
    11941 atgttgaata ctcatattct tcctt
//
