LOCUS       sequence-304350-        2442 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian cell surface expression of His- and FLAG-tagged 
            full-length protein for binding and signaling assays.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2442)
  AUTHORS   Wojtowicz WM, Vielmetter J, Fernandes RA, Siepe DH, Eastman CL, 
            Chisholm GB, Cox S, Klock H, Anderson PW, Rue SM, Miller JJ, Glaser 
            SM, Bragstad ML, Vance J, Lam AW, Lesley SA, Zinn K, Garcia KC
  TITLE     A Human IgSF Cell-Surface Interactome Reveals a Complex Network of 
            Protein-Protein Interactions.
  JOURNAL   Cell. 2020 Aug 20;182(4):1027-1043.e17. doi: 
            10.1016/j.cell.2020.07.025.
  PUBMED    32822567
REFERENCE   2  (bases 1 to 2442)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2020.07"; journalName: "Cell"; date: "2020-08-20- 
            20"; volume: "182"; issue: "4"; pages: "1027-1043.e17"
COMMENT     Sequence Label: pTT3-SP-6xHis-NTRK1(FL)-FLAG
FEATURES             Location/Qualifiers
     source          1..2442
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             106..123
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             2419..2442
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 atgcttagag gtggaagaag aggacagctc ggctggcatt cttgggctgc tggacctgga
       61 tctctgctgg cttggctgat tcttgcttct gccggcgctg gaagccacca ccatcaccat
      121 catgctccct gtcctgatgc ctgttgtcct cacggaagca gcggcctgag atgtacaaga
      181 gatggcgccc tggacagcct gcatcatctg cctggtgccg agaatctgac cgagctgtac
      241 atcgagaacc agcagcatct gcagcacctg gaactgagag atctgagagg cctgggcgag
      301 ctgagaaacc tgacaatcgt gaagtccggg ctgagattcg tggcccctga cgcctttcac
      361 ttcaccccta gactgagccg gctgaacctg agcttcaatg ccctggaatc cctgagctgg
      421 aaaaccgtgc agggcctgtc tctgcaagag ctggtgctgt ctggcaatcc tctgcactgt
      481 agctgtgccc tgagatggct gcagagatgg gaagaagaag gcctcggcgg agtccctgag
      541 cagaaactgc aatgtcatgg ccagggacct ctggctcaca tgcctaatgc aagctgtggc
      601 gtgcccacac tgaaggtgca ggttccaaat gcctctgtgg acgtgggaga tgacgtgctg
      661 ctgagatgcc aggttgaagg cagaggactg gaacaggccg gatggatcct gacagagctg
      721 gaacagagcg ccaccgtgat gaagtctggt ggactgcctt ctctgggcct gacactggcc
      781 aatgtgacca gcgacctgaa ccggaagaat gtgacctgct gggccgagaa cgatgtgggc
      841 agagctgaag tgtccgtgca agtgaacgtg tcattccctg ccagcgttca gctgcacaca
      901 gccgtggaaa tgcaccactg gtgtatccct ttcagcgtgg acggacagcc agcacctagc
      961 ctgcgttggc tgtttaacgg cagcgtgctg aacgagacaa gcttcatctt caccgagttt
     1021 ctggaaccag ccgccaacga aacagtgcgg catggctgtc tgagactgaa ccagcctaca
     1081 cacgtgaaca acggcaacta caccctgctg gccgccaatc cttttggaca ggcctctgcc
     1141 tctatcatgg ccgccttcat ggacaacccc ttcgagttca accccgagga tcccattcct
     1201 gtgtctttca gccccgtgga caccaatagc acaagcggcg accccgtgga aaagaaggat
     1261 gagacacctt ttggcgtgtc cgtggccgtt ggactggctg tgtttgcctg tctgttcctg
     1321 agcacactgc tgcttgtgct gaacaagtgc ggcagaagaa acaagttcgg catcaacaga
     1381 cctgccgtgc tggctcctga agatggactg gcaatgagcc tgcactttat gaccctcggc
     1441 ggcagcagcc tgtctcctac tgaaggcaaa ggttctggac tgcagggcca catcatcgag
     1501 aatccccagt acttcagcga cgcctgcgtg caccacatca agagaagaga catcgtgctg
     1561 aagtgggaac tcggcgaggg cgcctttgga aaagtgtttc tggccgagtg ccacaacctg
     1621 ctgcctgagc aggataagat gctggtggcc gtgaaggccc tgaaagaagc ctctgaaagc
     1681 gccagacagg acttccagag agaggccgaa ctgctgacaa tgctgcaaca ccagcacatc
     1741 gtgcggttct tcggcgtgtg tacagaaggc agacccctgc tgatggtgtt cgagtacatg
     1801 cggcacggcg atctgaacag attcctgaga agccacggac ctgacgccaa acttctggct
     1861 ggcggagaag atgtggctcc tggacctctt ggactgggac agctgctggc agttgcttct
     1921 caagtggccg ctggaatggt gtatctggcc ggactgcact tcgtgcacag agatctggcc
     1981 accagaaatt gcctcgtcgg acagggcctc gtggtcaaga tcggcgattt cggcatgagc
     2041 cgggacatct acagcaccga ctattacaga gtcggcggac ggaccatgct gcccattaga
     2101 tggatgcctc cagagagcat cctgtaccgg aagttcacca ccgagagcga cgtgtggtct
     2161 ttcggagtgg tgctgtggga gatcttcacc tacggcaagc agccctggta tcagctgagc
     2221 aacacagagg ccatcgactg catcacccag ggaagagaac tggaaagacc cagagcctgt
     2281 cctcctgagg tgtacgccat catgagaggc tgttggcaga gagagcctca gcagagacac
     2341 agcatcaagg acgtgcacgc tagactgcag gcactggctc aggctcctcc agtgtacctg
     2401 gatgtgctcg gcggctccga ctacaaggac gacgatgata ag
//
