LOCUS       sequence-304352-        3468 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian cell surface expression of His- and FLAG-tagged 
            full-length protein for binding and signaling assays.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3468)
  AUTHORS   Wojtowicz WM, Vielmetter J, Fernandes RA, Siepe DH, Eastman CL, 
            Chisholm GB, Cox S, Klock H, Anderson PW, Rue SM, Miller JJ, Glaser 
            SM, Bragstad ML, Vance J, Lam AW, Lesley SA, Zinn K, Garcia KC
  TITLE     A Human IgSF Cell-Surface Interactome Reveals a Complex Network of 
            Protein-Protein Interactions.
  JOURNAL   Cell. 2020 Aug 20;182(4):1027-1043.e17. doi: 
            10.1016/j.cell.2020.07.025.
  PUBMED    32822567
REFERENCE   2  (bases 1 to 3468)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2020.07"; journalName: "Cell"; date: "2020-08-20- 
            20"; volume: "182"; issue: "4"; pages: "1027-1043.e17"
COMMENT     Sequence Label: pTT3-SP-6xHis-TIE1(FL)-FLAG
FEATURES             Location/Qualifiers
     source          1..3468
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             70..87
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             3445..3468
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 atggtttgga gagttcctcc attcctgctg cctatcctgt tcctggcctc tcacgttgga
       61 gccggatctc accatcacca ccatcatgcc gtggatctga ccctgctggc caacctgaga
      121 ctgaccgatc ctcagagatt cttcctgacc tgtgtgtctg gcgaagccgg cgctggaaga
      181 ggatctgatg cttggggacc tcctctgctg ctggaaaagg acgacagaat cgtgcggaca
      241 cctcctggac ctccactgag actggccaga aatggcagcc accaagtgac cctgagaggc
      301 ttcagcaagc cttctgatct cgtgggcgtg ttctcttgtg ttggaggcgc tggcgctcgg
      361 agaaccagag tgatctatgt gcacaacagc cctggcgctc atctgctgcc tgacaaagtg
      421 acccacaccg tgaacaaggg cgatacagcc gtgctgtccg ccagagtgca caaagaaaag
      481 cagaccgacg tgatctggaa gtccaacggc agctacttct acaccctgga ttggcacgag
      541 gcccaggacg gaagatttct gctgcagctg cctaacgtgc agcctcctag ctctggcatc
      601 tacagcgcca catacctgga agcaagccct ctgggcagcg ccttcttcag actgatcgtt
      661 agaggatgcg gagccggcag atggggccct ggatgtacaa aagaatgccc tggctgtctg
      721 cacggcggcg tgtgtcacga tcatgatggc gagtgtgtgt gcccacctgg ctttacaggc
      781 accagatgtg aacaggcctg cagagagggc agattcggcc agtcttgtca agagcagtgt
      841 cccggcatct ctggctgcag aggcctgacc ttttgcctgc ctgatcctta cggctgtagc
      901 tgtggatctg gatggcgggg ctctcagtgt caagaggctt gtgcccctgg acactttggc
      961 gctgattgca gactgcagtg ccagtgccag aatggcggca cctgtgatag attcagcggc
     1021 tgtgtgtgtc cttctggctg gcatggcgtg cactgcgaga agtccgatag aatccctcag
     1081 atcctgaaca tggccagcga gctggaattc aacctggaaa ccatgcctcg gatcaactgc
     1141 gccgctgccg gaaatccatt tcctgtgcgg ggatccatcg agctgagaaa gcctgatggc
     1201 accgtgctgc tgagcaccaa ggccatcgtg gaacctgaga aaaccaccgc cgagttcgag
     1261 gtgccaagac tggttctggc cgatagcggc ttctgggagt gtagagtgtc tacctctggc
     1321 ggccaggaca gcagacggtt caaagtgaat gtgaaggtgc cacctgtgcc tctggcagcc
     1381 cctagactgc tgacaaaaca gagcagacag ctggtggtgt cccctctggt gtcttttagc
     1441 ggagatggcc ctatcagcac agtgcggctg cactacagac cccaggatag cacaatggac
     1501 tggtctacca tcgtggtgga ccccagcgag aacgtgacac tgatgaacct gaggcctaag
     1561 accggctaca gcgtcagagt tcaactgagc agacctggcg aaggcggaga aggcgcatgg
     1621 ggaccaccta ctctgatgac cacagattgc cctgagcctc tgctccagcc ttggctggaa
     1681 ggatggcatg tggaaggcac cgacagactg agagtgtctt ggagtctgcc actggtgcca
     1741 ggacctctcg ttggagatgg ctttctgctg agactgtggg acggcaccag aggccaagag
     1801 agaagagaga acgtcagcag ccctcaggcc agaacagctc tgctgacagg actgacacct
     1861 ggcacacact accagctgga tgtgcagctg taccactgta cactgctggg acctgcctct
     1921 cctcctgctc atgttctgtt gcctccatct ggccctccag ctcctagaca tctgcatgcc
     1981 caggctctga gcgatagcga gatccagctg acatggaagc acccagaagc tctgcccgga
     2041 ccaatcagca aatacgtggt ggaagtgcag gttgccggcg gagctggcga tcctctgtgg
     2101 attgatgtgg acagacccga ggaaaccagc accatcatca ggggcctgaa cgccagcacc
     2161 agatacctgt tcagaatgcg ggcctctatc caaggcctcg gcgattggtc taacaccgtg
     2221 gaagagtcta ccctcggcaa cggactgcag gctgaaggac ctgtgcaaga aagcagagcc
     2281 gccgaggaag gactggacca gcaactgatt ctggctgtcg tgggctccgt gtctgccaca
     2341 tgtctgacaa ttctggccgc actgctgacc ctcgtgtgca tcagaagaag ctgcctgcac
     2401 agacggcgga cctttaccta tcagtctggc tccggcgagg aaacaatcct gcagtttagc
     2461 agcggcaccc tgacactgac cagaaggcca aaactgcagc ccgagccact gagctatcct
     2521 gtgctggaat gggaagatat caccttcgag gacctgatcg gcgagggcaa ctttggccaa
     2581 gtgatccggg ccatgatcaa gaaagacggc ctgaagatga acgccgccat caagatgctg
     2641 aaagagtacg cctccgagaa cgaccacaga gactttgccg gcgaactgga agtgctgtgc
     2701 aagctgggcc accatcctaa catcatcaat ctgctgggcg cctgcaagaa ccggggctac
     2761 ctgtatatcg ccatcgagta cgccccttac ggaaacctgc tggactttct gcggaagtcc
     2821 agagtgctgg aaacagaccc tgcctttgcc agagagcacg gcacagcttc tacactgagt
     2881 agccggcagc tgctgagatt tgcctctgat gccgccaacg gcatgcagta cctgagcgag
     2941 aagcagttca ttcaccgcga cctggccgct agaaatgtgc tcgtgggaga gaacctggcc
     3001 agcaagatcg ccgattttgg cctgagtaga ggcgaagagg tgtacgtgaa gaaaaccatg
     3061 ggcagactgc ccgtgcggtg gatggccatt gagagcctga attacagcgt gtacaccacc
     3121 aagagcgacg tgtggtcctt tggagtgctg ctgtgggaga tcgtgtctct cggcggcaca
     3181 ccttactgtg gcatgacatg tgccgagctg tacgagaaac tgccccaggg ctacagaatg
     3241 gaacagccca gaaactgcga cgacgaagtg tacgagctga tgcggcagtg ttggagagac
     3301 agaccttacg agaggcctcc attcgctcag atcgctctgc agctgggaag aatgctggaa
     3361 gccagaaagg cctacgtgaa catgtccctg ttcgagaact tcacctacgc cggaatcgac
     3421 gccacagccg aagaggccgg cagcgactac aaggacgacg acgataag
//
