LOCUS       sequence-304354-        1305 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian cell surface expression of His- and FLAG-tagged 
            full-length protein for binding and signaling assays.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1305)
  AUTHORS   Wojtowicz WM, Vielmetter J, Fernandes RA, Siepe DH, Eastman CL, 
            Chisholm GB, Cox S, Klock H, Anderson PW, Rue SM, Miller JJ, Glaser 
            SM, Bragstad ML, Vance J, Lam AW, Lesley SA, Zinn K, Garcia KC
  TITLE     A Human IgSF Cell-Surface Interactome Reveals a Complex Network of 
            Protein-Protein Interactions.
  JOURNAL   Cell. 2020 Aug 20;182(4):1027-1043.e17. doi: 
            10.1016/j.cell.2020.07.025.
  PUBMED    32822567
REFERENCE   2  (bases 1 to 1305)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2020.07"; journalName: "Cell"; date: "2020-08-20- 
            20"; volume: "182"; issue: "4"; pages: "1027-1043.e17"
COMMENT     Sequence Label: pTT3-SP-6xHis-PVR(FL)-FLAG
FEATURES             Location/Qualifiers
     source          1..1305
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             79..96
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             1282..1305
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 atggctagag ctatggctgc tgcttggcct ctgctgctgg ttgctctgct ggttctttct
       61 tggcctccac ctggctctca ccaccaccat caccatggaa ctggggatgt tgtggtgcag
      121 gctcctacac aggtgccagg ctttctgggc gattctgtga ccctgccttg ctacctgcaa
      181 gtgcccaaca tggaagtgac ccacgtgtcc cagctgacat gggccagaca tggcgaatct
      241 ggcagcatgg ccgtgtttca ccagacacag ggccctagct acagcgagag caagagactg
      301 gaattcgtgg ccgccagact gggcgctgaa ctgagaaatg ccagcctgag aatgttcggc
      361 ctgagagtgg aagatgaggg caactacacc tgtctgttcg tgacctttcc acagggcagc
      421 agaagcgtgg acatctggct gagagtgctg gccaaacctc agaataccgc cgaggtgcag
      481 aaagtgcagc tgacaggcga acctgtgcct atggccagat gtgtgtctac tggtggcaga
      541 cctcctgctc agatcacctg gcattctgat ctcggcggca tgcccaatac ttctcaggtg
      601 cccggattcc tgagcggcac agtgacagtg acaagcctgt ggatcctggt gcctagctct
      661 caggtggacg gaaagaacgt gacctgcaag gtggaacacg agagcttcga gaagcctcag
      721 ctgctgaccg tgaacctgac cgtgtactat cctcctgagg tgtccatcag cggctacgac
      781 aacaactggt atctgggcca gaacgaggcc acactgacct gcgacgccag aagcaatcct
      841 gagcctaccg gctacaactg gtccaccaca atgggccctc tgcctccatt tgctgttgct
      901 caaggcgccc agctgctcat cagacccgtg gacaagccca tcaacacaac cctgatctgc
      961 aacgtgacaa acgccctggg agccagacag gctgagctga cagtgcaagt gaaagaggga
     1021 cctcctagcg agcacagcgg catcagcaga aacgccatca tctttctggt gctgggcatc
     1081 ctggtgtttc tgatcctgct cggcatcggc atctacttct actggtccaa gtgcagccgg
     1141 gaagtgctgt ggcattgtca cctgtgtcct agctctacag agcatgccag cgcctctgcc
     1201 aatggccatg tgtcttatag cgccgtgtcc agagagaaca gcagctccca ggatcctcag
     1261 acagagggca caagaggcag cgactacaag gacgacgacg acaag
//
