LOCUS       sequence-304356-        1179 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian cell surface expression of His- and FLAG-tagged 
            full-length protein for binding and signaling assays.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1179)
  AUTHORS   Wojtowicz WM, Vielmetter J, Fernandes RA, Siepe DH, Eastman CL, 
            Chisholm GB, Cox S, Klock H, Anderson PW, Rue SM, Miller JJ, Glaser 
            SM, Bragstad ML, Vance J, Lam AW, Lesley SA, Zinn K, Garcia KC
  TITLE     A Human IgSF Cell-Surface Interactome Reveals a Complex Network of 
            Protein-Protein Interactions.
  JOURNAL   Cell. 2020 Aug 20;182(4):1027-1043.e17. doi: 
            10.1016/j.cell.2020.07.025.
  PUBMED    32822567
REFERENCE   2  (bases 1 to 1179)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2020.07"; journalName: "Cell"; date: "2020-08-20- 
            20"; volume: "182"; issue: "4"; pages: "1027-1043.e17"
COMMENT     Sequence Label: pTT3-SP-6xHis-KIR2DL5(FL)-FLAG
FEATURES             Location/Qualifiers
     source          1..1179
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             70..90
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=7xHis
                     /translation="HHHHHHH"
     CDS             1156..1179
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 atgtctctga tggttatttc tatggcctgc gtgggattct tcctgctgca aggcgcttgg
       61 acaggctctc accaccatca ccatcatcac gaaggcggcc aggataagcc tctgctgtct
      121 gcttggcctt ctgctgtggt tcctagaggc ggacacgtga ccctgctgtg tagaagcaga
      181 ctgggcttca ccatcttcag cctgtacaaa gaggacggcg tccccgttcc tgagctgtac
      241 aacaagatct tctggaagtc catcctgatg ggccccgtga caccagctca cgccggcaca
      301 tatagatgca gaggcagcca tcctagaagc cccattgagt ggtccgctcc tagcaaccct
      361 ctggtcatcg tggtcaccgg cctgtttggc aaaccttctc tgtctgccca gcctggacct
      421 acagtgcgga caggcgaaaa tgtgaccctg agctgcagca gcagatccag cttcgacatg
      481 taccacctga gcagagaggg cagagcccac gaacctagac tgcctgctgt gccttccgtg
      541 aatggcacct tccaggccga ttttccactg ggacctgcca cacacggcgg cacctacaca
      601 tgttttggca gcctgcacga cagcccctac gagtggtctg atccttctga tccactgctg
      661 gtgtccgtga ccggcaatag cagtagcagc agctctagcc ctaccgagcc tagcagcaag
      721 accggcattc ggagacatct gcacatcctg atcggcacct ctgtggccat catcctgttt
      781 attatcctgt tcttctttct gctccactgt tgctgctcca acaagaaaaa cgccgccgtg
      841 atggatcaag agcctgccgg cgatagaacc gtgaacagag aggactctga cgaccaggat
      901 cctcaagaag tgacatacgc ccagctggat cactgcgtgt tcacccagac caagatcaca
      961 agccccagcc agaggcctaa gacacctcct accgacacca caatgtacat ggaactgccc
     1021 aacgccaagc ctagatctct gagccctgct cacaaacacc acagccaggc tctgagaggc
     1081 agctccagag aaacaacagc cctgagccag aacagagtgg ccagctctca tgtgcctgcc
     1141 gccggaatcg gcagcgacta caaggacgac gacgacaag
//
