LOCUS       sequence-304360-        1098 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian cell surface expression of His- and FLAG-tagged 
            full-length protein for binding and signaling assays.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1098)
  AUTHORS   Wojtowicz WM, Vielmetter J, Fernandes RA, Siepe DH, Eastman CL, 
            Chisholm GB, Cox S, Klock H, Anderson PW, Rue SM, Miller JJ, Glaser 
            SM, Bragstad ML, Vance J, Lam AW, Lesley SA, Zinn K, Garcia KC
  TITLE     A Human IgSF Cell-Surface Interactome Reveals a Complex Network of 
            Protein-Protein Interactions.
  JOURNAL   Cell. 2020 Aug 20;182(4):1027-1043.e17. doi: 
            10.1016/j.cell.2020.07.025.
  PUBMED    32822567
REFERENCE   2  (bases 1 to 1098)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2020.07"; journalName: "Cell"; date: "2020-08-20- 
            20"; volume: "182"; issue: "4"; pages: "1027-1043.e17"
COMMENT     Sequence Label: pTT3-SP-6xHis-KIR2DL1(FL)-FLAG
FEATURES             Location/Qualifiers
     source          1..1098
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             70..90
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=7xHis
                     /translation="HHHHHHH"
     CDS             1075..1098
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 atgtctctgc tggttgtttc tatggcctgc gtgggattct tcctgctgca aggcgcttgg
       61 cctggatctc accaccatca ccatcatcac gagggcgtgc acagaaagcc tagcctgctt
      121 gctcatcccg gacctctggt caagagcgaa gagacagtga tcctgcagtg ttggagcgac
      181 gtgatgttcg agcactttct gctgcaccgc gagggcatgt tcaacgacac actgagactg
      241 atcggcgagc accacgatgg cgtgtccaag gccaatttca gcatcagcag aatgacccag
      301 gacctggccg gcacctacag atgttatggc tctgtgacac acagccccta ccaggtgtca
      361 gcccctagcg atcctctgga catcgtgatc atcggcctgt acgagaagcc aagcctgtct
      421 gcacagcctg gacctacagt gctggccggc gaaaatgtga ccctgagctg tagcagccgg
      481 tccagctacg atatgtacca cctgagcaga gagggcgaag cccacgaaag aaggctgcct
      541 gccggaccta aagtgaacgg caccttccag gccgattttc cactgggacc tgccacacac
      601 ggcggcacat atagatgctt cggcagcttc cacgacagcc cttacgagtg gtctaagagc
      661 agcgatccac tgctggtgtc cgtgaccggc aatcctagca atagctggcc ttctccaacc
      721 gagcctagca gcaagacagg caaccccaga catctgcaca tcctgatcgg caccagcgtg
      781 gtcatcatcc tgttcatcct gctgttcttt ctgctccaca gatggtgcag caacaagaaa
      841 aacgccgccg tgatggatca agagagcgcc ggcaatagaa ccgccaacag cgaggattcc
      901 gacgagcagg accctcaaga agtgacctac acacagctga accactgcgt gttcacccag
      961 cggaagatca ccagacctag ccagaggcct aagacacctc ctaccgacat catcgtgtac
     1021 accgagctgc ctaacgccga gagcagatcc aaggtggtgt cttgccctgg cagcgactac
     1081 aaggacgacg acgataag
//
