LOCUS       sequence-304362-        2289 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian cell surface expression of His- and FLAG-tagged 
            full-length protein for binding and signaling assays.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2289)
  AUTHORS   Wojtowicz WM, Vielmetter J, Fernandes RA, Siepe DH, Eastman CL, 
            Chisholm GB, Cox S, Klock H, Anderson PW, Rue SM, Miller JJ, Glaser 
            SM, Bragstad ML, Vance J, Lam AW, Lesley SA, Zinn K, Garcia KC
  TITLE     A Human IgSF Cell-Surface Interactome Reveals a Complex Network of 
            Protein-Protein Interactions.
  JOURNAL   Cell. 2020 Aug 20;182(4):1027-1043.e17. doi: 
            10.1016/j.cell.2020.07.025.
  PUBMED    32822567
REFERENCE   2  (bases 1 to 2289)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2020.07"; journalName: "Cell"; date: "2020-08-20- 
            20"; volume: "182"; issue: "4"; pages: "1027-1043.e17"
COMMENT     Sequence Label: pTT3-SP-6xHis-ISLR2(FL)-FLAG
FEATURES             Location/Qualifiers
     source          1..2289
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             61..78
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             2266..2289
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 atgtttccac tgagagcact ttggctcgtg tgggcccttc ttggagttgc cggcggatct
       61 caccaccacc atcaccactc ttgtcctgag ccttgcgcct gcgtggacaa atatgcccac
      121 cagttcgccg actgcgccta caaagaactg agagaggtgc cagagggcct gcctgccaat
      181 gtgacaacac tgagcctgag cgccaacaag atcaccgtgc tgagaagagg cgccttcgcc
      241 gatgtgacac aagtgacatc tctgtggctg gcccacaacg aagtgcgaac agttgaacct
      301 ggtgctctgg ccgtgctgtc ccagctgaag aatctggacc tgagccacaa cttcatcagc
      361 agcttccctt ggagcgacct gagaaatctg tctgccctgc agctgctgaa gatgaaccac
      421 aacagactgg gcagcctgcc tagagatgct ctgggagcac tgcctgacct gcggagcctg
      481 agaatcaaca acaaccggct gagaacactg gccccaggca cctttgatgc cctgtctgct
      541 ctgagccatc tgcagctgta tcacaaccct ttccactgcg gctgtggact tgtgtggctt
      601 caagcctggg ccgcctctac aagagtgtct ctgccagagc ctgactctat cgcctgtgct
      661 tctccaccag ctctgcaggg cgttccagtg tatagactgc ctgctctgcc ttgcgctcct
      721 ccttctgtgc atctttctgc cgagcctcca ctggaagcac ctggaacacc tcttagagcc
      781 ggactggcct tcgtgctgca ctgtattgcc gatggacacc ccacacctag actgcagtgg
      841 cagcttcaga ttcctggcgg cacagtggtg ctggaacctc cagttctgtc tggcgaagat
      901 gatggcgtgg gagcagaaga aggcgaaggc gaaggcgacg gggatctgct gacacagaca
      961 caggctcaga cacccacacc agctccagct tggcctgctc ctcctgccac accaagattt
     1021 ctggccctgg ccaatggaag cctgctggtt cctctgctgt ctgccaaaga ggccggcgtc
     1081 tacacctgta gagcccacaa tgagctgggc gccaatagca cctctattag agtggccgtg
     1141 gccgccacag gacctccaaa acatgctcct ggtgctggcg gagaacctga tggacaggcc
     1201 cctacaagcg agagaaagag cactgctaaa ggcagaggca acagcgtgct gccttctaag
     1261 cccgagggca agatcaaagg ccagggactc gccaaagtgt ccatcctggg agagacagag
     1321 actgagcccg aagaggatac ctctgagggc gaagaggccg aggatcagat tctggctgat
     1381 cccgccgaag aacagagatg cggcaatggc gatcccagca gatacgtgtc caaccacgcc
     1441 ttcaatcaga gcgccgagct gaagcctcac gtgttcgaac tgggagtgat tgccctggat
     1501 gtggccgaaa gggaagccag agttcagctg acaccactgg ctgctagatg gggacctgga
     1561 ccaggtggcg caggcggagc acctagacct ggaagaaggc ctctgagact gctgtatctg
     1621 tgtccagctg gtggcggagc tgctgtgcaa tggtcaagag tggaagaggg cgtgaacgcc
     1681 tactggttta gaggcctgag gcctggcacc aactacagcg tttgtctggc tcttgctggc
     1741 gaggcctgtc acgtgcaagt ggtgttcagc accaagaaag agctgcccag cctgctcgtg
     1801 attgtggccg tgtctgtttt tctgctggtc ctggctacag tgccactgct gggagctgct
     1861 tgttgccatc tgctggccaa acatcccggc aagccctaca gactgattct gaggccacag
     1921 gctcccgatc ctatggaaaa gagaatcgcc gccgacttcg accccagagc cagctacctg
     1981 gaaagcgaga agtcttatcc agccggcgga gaagctggcg gcgaagaacc agaagatgtg
     2041 caaggcgagg gcctcgatga agatgctgaa caaggcgatc ctagcggcga cctgcagaga
     2101 gaagaatctc tggccgcctg tagcctggtg gaaagccaga gcaaggccaa tcaagaggaa
     2161 ttcgaggccg gcagcgagta ctctgataga cttccactgg gagccgaggc cgtgaatatc
     2221 gcccaagaga tcaacggcaa ctacagacag accgctggcg gcagcgacta caaggacgac
     2281 gatgataag
//
