LOCUS       sequence-304385-       11799 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  CRISPR reporter for mRNA quantification, integrative plasmid into 
            NPR2 gene.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11799)
  AUTHORS   Brion C, Lutz SM, Albert FW
  TITLE     Simultaneous quantification of mRNA and protein in single cells 
            reveals post-transcriptional effects of genetic variation.
  JOURNAL   Elife. 2020 Nov 16;9. pii: 60645. doi: 10.7554/eLife.60645.
  PUBMED    33191917
REFERENCE   2  (bases 1 to 11799)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2020-11-16"; volume: "9. pii: 60645. doi"; pages: " 
            10.7554/eLife.60645"
COMMENT     Sequence Label: Plas-CRISPR_reporter
FEATURES             Location/Qualifiers
     source          1..11799
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    complement(9..42)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     gene            complement(105..1461)
                     /label=kanMX
                     /note="yeast selectable marker conferring kanamycin 
                     resistance (Wach et al., 1994)"
     terminator      complement(105..302)
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
     CDS             complement(308..1117)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MGKEKTHVSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     1031..1050
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     promoter        complement(1118..1461)
                     /label=TEF promoter
                     /note="Ashbya gossypii TEF promoter"
     protein_bind    complement(1516..1549)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        1601..1705
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1706..1774,1775..2566)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1924..1943)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2737..3325
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3479..3496
                     /label=L4440
                     /note="L4440 vector, forward primer"
     promoter        join(3649..3654,3655..3672,3673..3679)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3687..3703
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     3692..3714
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     3711..3727
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3711..3727
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        3748..3766
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     terminator      complement(3790..4037)
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     primer_bind     4003..4021
                     /label=CYC1
                     /note="CYC1 transcription termination signal, reverse 
                     primer"
     CDS             complement(4053..4202)
                     /codon_start=1
                     /product="tetrameric repeat of the minimal activation 
                     domain of herpes simplex virus VP16 (Beerli et al., 1998)"
                     /label=VP64
                     /translation="DALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDALDDF
                     DLDML"
     CDS             complement(join(4239..5822,5823..5825,5826..8312,8313..8315,
                     8316..8339))
                     /codon_start=1
                     /product="catalytically dead mutant of the Cas9 
                     endonuclease from the Streptococcus pyogenes Type II 
                     CRISPR/Cas system"
                     /label=dCas9
                     /note="RNA-guided DNA-binding protein that lacks 
                     endonuclease activity due to the D10A mutation in the RuvC 
                     catalytic domain and the H840A mutation in the HNH 
                     catalytic domain"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             complement(8349..8369)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(9077..9787)
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(9355..9374)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     9624..9642
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
ORIGIN
        1 atcacctaat aacttcgtat agcatacatt atacgaagtt atattaaggg ttctcgagag
       61 ctcgttttcg acactggatg gcggcgttag tatcgaatcg acagcagtat agcgaccagc
      121 attcacatac gattgacgca tgatattact ttctgcgcac ttaacttcgc atctgggcag
      181 atgatgtcga ggcgaaaaaa aatataaatc acgctaacat ttgattaaaa tagaacaact
      241 acaatataaa aaaactatac aaatgacaag ttcttgaaaa caagaatctt tttattgtca
      301 gtactgatta gaaaaactca tcgagcatca aatgaaactg caatttattc atatcaggat
      361 tatcaatacc atatttttga aaaagccgtt tctgtaatga aggagaaaac tcaccgaggc
      421 agttccatag gatggcaaga tcctggtatc ggtctgcgat tccgactcgt ccaacatcaa
      481 tacaacctat taatttcccc tcgtcaaaaa taaggttatc aagtgagaaa tcaccatgag
      541 tgacgactga atccggtgag aatggcaaaa gcttatgcat ttctttccag acttgttcaa
      601 caggccagcc attacgctcg tcatcaaaat cactcgcatc aaccaaaccg ttattcattc
      661 gtgattgcgc ctgagcgaga cgaaatacgc gatcgctgtt aaaaggacaa ttacaaacag
      721 gaatcgaatg caaccggcgc aggaacactg ccagcgcatc aacaatattt tcacctgaat
      781 caggatattc ttctaatacc tggaatgctg ttttgccggg gatcgcagtg gtgagtaacc
      841 atgcatcatc aggagtacgg ataaaatgct tgatggtcgg aagaggcata aattccgtca
      901 gccagtttag tctgaccatc tcatctgtaa catcattggc aacgctacct ttgccatgtt
      961 tcagaaacaa ctctggcgca tcgggcttcc catacaatcg atagattgtc gcacctgatt
     1021 gcccgacatt atcgcgagcc catttatacc catataaatc agcatccatg ttggaattta
     1081 atcgcggcct cgaaacgtga gtcttttcct tacccatggt tgtttatgtt cggatgtgat
     1141 gtgagaactg tatcctagca agattttaaa aggaagtata tgaaagaaga acctcagtgg
     1201 caaatcctaa ccttttatat ttctctacag gggcgcggcg tggggacaat tcaacgcgtc
     1261 tgtgagggga gcgtttccct gctcgcaggt ctgcagcgag gagccgtaat ttttgcttcg
     1321 cgccgtgcgg ccatcaaaat gtatggatgc aaatgattat acatggggat gtatgggcta
     1381 aatgtacggg cgacagtcac atcatgcccc tgagctgcgc acgtcaagac tgtcaaggag
     1441 ggtattctgg gcctccatgt cgctggccgg gtgacccggc ggggacgagg caagctaaac
     1501 agatctctag acctaataac ttcgtatagc atacattata cgaagttata ttaagggttg
     1561 tcgacgctcg acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt
     1621 atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct
     1681 tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc
     1741 cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa
     1801 agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg
     1861 taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt
     1921 tctgctatgt ggcgcggtat tatcccgtat tgacgccggg caagagcaac tcggtcgccg
     1981 catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac
     2041 ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc
     2101 ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa
     2161 catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc
     2221 aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt
     2281 aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga
     2341 taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa
     2401 atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa
     2461 gccctcccgt atcgtagtta tctacacgac gggcagtcag gcaactatgg atgaacgaaa
     2521 tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt
     2581 ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt
     2641 gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg
     2701 agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt
     2761 aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca
     2821 agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac
     2881 tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac
     2941 atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct
     3001 taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg
     3061 gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca
     3121 gcgtgagcat tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt
     3181 aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggga acgcctggta
     3241 tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc
     3301 gtcagggggg ccgagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc
     3361 cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa
     3421 ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag
     3481 cgagtcagtg agcgaggaag cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg
     3541 ttggccgatt cattaatgca gctggcacga caggtttccc gactggaaag cgggcagtga
     3601 gcgcaacgca attaatgtga gttacctcac tcattaggca ccccaggctt tacactttat
     3661 gcttccggct cctatgttgt gtggaattgt gagcggataa caatttcaca caggaaacag
     3721 ctatgaccat gattacgcca agctcggaat taaccctcac taaagggaac aaaagctggg
     3781 taccgggccg caaattaaag ccttcgagcg tcccaaaacc ttctcaagca aggttttcag
     3841 tataatgtta catgcgtaca cgcgtctgta cagaaaaaaa agaaaaattt gaaatataaa
     3901 taacgttctt aatactaaca taactataaa aaaataaata gggacctaga cttcaggttg
     3961 tctaactcct tccttttcgg ttagagcgga tgtgggggaa gggcgtgaat gtaagcgtga
     4021 cataactaat tacatgactc gagttcctag gaaagcatgt ctaagtcgaa atcgtccaga
     4081 gcgtctgatc caagcatatc caggtcaaaa tcatctaagg cgtcacttcc aagcatgtcg
     4141 aggtcaaagt catctagcgc atcagaaccc agcatatcca agtcgaagtc gtccaaagcg
     4201 tcagaacctc caccgtcgac tccagatcta ccagaaccat caccacctaa ttgagacaag
     4261 tcgattctag tttcgtacaa accagtaata gattggtgga tcaaagtagc gtccaaaact
     4321 tctttggtag aggtgtatct ctttctgtcg atagtggtat cgaaatactt aaaagcagct
     4381 ggagcaccca agttagtcaa agtgaacaag tgaatgatgt tttcagcttg ttctctaatt
     4441 ggcttatctc tgtgcttgtt gtaagctgac aaaactttgt ccaaattggc gtcagccaaa
     4501 atgactcttt tggaaaattc ggaaatttgt tcaatgattt cgtccaagta gtgcttgtgt
     4561 tgttcgacaa ataattgctt ttgttcgtta tcttcaggag aacctttcaa tttttcgtag
     4621 tgggaggcca agtataagaa attaacgtac ttggatggca aagccaattc attacccttt
     4681 tgtaattcac cggcagaagc caacattctc tttctaccat tttccaattc gaacaaactg
     4741 tactttggca acttaatgat caagtccttc ttgacttctt tgtaaccctt ggcttccaaa
     4801 aagtcgatag gattcttttc gaatgaggat ctttccatga tagtgatacc taataattct
     4861 ttgacggact tcaacttctt actcttaccc ttttcgacct tagcaacaac caaaacagag
     4921 taagcaacag ttggagaatc aaaaccaccg tacttttttg ggtcccagtc tttttttcta
     4981 gcgatcaact tatcggagtt tctcttaggc aaaatggatt ctttggagaa accaccagtt
     5041 tgaacttcgg tctttttgac gatgtttact tgtggcatgg acaatacctt tctaacagtg
     5101 gcaaaatctc tacccttatc ccaaacgatt tcacctgttt caccattagt ttcaatcaat
     5161 ggtctttttc taatttcacc gttggccaaa gtgatttcgg tcttaaagaa attcataata
     5221 ttggagtaga agaagtattt ggcagtagct ttaccaattt cttgttcgga cttggcgatc
     5281 atctttctaa cgtcgtaaac cttgtaatca ccgtagacaa attcggattc caactttgga
     5341 tacttcttaa tcaaggcagt accaacaaca gcattcaagt aagcatcatg tgcatgatgg
     5401 tagttgttga tttctctgac cttgtagaat tggaagtcct ttctgaaatc ggaaaccaac
     5461 ttggacttca aggtaatgac cttaacttct ctgatcaatt tgtcgttttc gtcgtactta
     5521 gtgttcattc tactatccaa aatttgagca acgtgcttgg tgatttgtct ggtttctact
     5581 aattgtcttt tgatgaaacc tgccttatcc aattcagaca aaccacctct ttcagccttg
     5641 gtcaaattgt caaactttct ttgggtgatc aacttggcgt tcaataattg tctccaatag
     5701 ttcttcattt tcttaacgac ttcttcagat ggaacgttat cggacttacc tctattctta
     5761 tcggatctag tcaagacctt gttgtcaatg gagtcatcct tcaaaaaaga ttgtggaacg
     5821 atagcgtcaa catcgtaatc agacaatctg ttgatgtcca attcttggtc aacgtacata
     5881 tctctaccgt tttgcaagta gtacaagtac aacttttcgt tttgtaattg ggtgttttca
     5941 actgggtgtt ctttcaaaat ttgggaaccc aattctttga taccttcttc gattctcttc
     6001 attctttctc tggagttctt ttgacccttt tgggtggttt gattttctct agccatttcg
     6061 ataacgatgt tttctggctt atgtctaccc ataactttga ccaattcgtc aacgacctta
     6121 acggtttgta aaataccctt cttaatagct ggagaaccag ccaagttagc gatatgttca
     6181 tgtaaagaat caccttgacc agagacttgg gccttttgta tatcttcttt gaaagtcaag
     6241 gaatcgtcgt ggattaattg catgaagttt ctgttagcga aaccatcaga cttcaaaaag
     6301 tccaaaatgg tcttaccgga ttgcttgtct ctgataccgt taatcaattt tctggacaat
     6361 ctaccccaac cagtgtatct tcttctcttt aattgcttca taaccttatc atcgaacaag
     6421 tgggcgtagg ttttcaatct ttcttcaatc atttctctat cttcaaacaa ggtcaatgtc
     6481 aagacaatat cttccaaaat atcttcgttt tcttcgttgt ccaagaaatc cttgtccttg
     6541 atgattttca acaagtcatg gtaagtacct aaagaggcat tgaatctatc ttcgacacca
     6601 gaaatttcaa cggagtcgaa gcattcgatt ttcttgaagt aatcttcttt taattgcttg
     6661 acagtaacct ttctgttggt cttgaacaac aaatcgacga tagccttctt ttgttcaccg
     6721 gataaaaaag ctggttttct cataccttcg gtgacgtact taaccttagt caattcgttg
     6781 tagacggtga agtattcgta caacaaagag tgctttggca aaaccttttc gtttggcaag
     6841 ttcttgtcga agttggtcat tctttcaata aaggattggg cagaagcacc cttatctaca
     6901 acttcttcga agttccaagg agtaatagtt tcttcggact ttctagtcat ccaggcaaat
     6961 cttgaattac ctctagccaa tggaccaacg tagtaaggga ttctgaaggt cagaatcttt
     7021 tcgatctttt ctctgttgtc cttcaagaat ggataaaaat cttcttgtct tctcaaaatt
     7081 gcgtgcaatt cacccaagtg aatttgatgt ggaatagaac cgttgtcgaa ggttctttgc
     7141 tttctcaaca aatcttctct gttcaacttg accaataatt cttcagtacc gtccatcttt
     7201 tccaagattg gcttgatgaa cttgtaaaat tcttcttgag aagcaccacc atcaatataa
     7261 ccggcgtaac cgttcttgga ttgatcgaag aaaatttctt tgtacttttc aggtaattgt
     7321 tgtctgacca aagccttcaa caaagtcaag tcttggtggt gttcatcgta tcttttgatc
     7381 atagaggcag acaatggagc cttagtaatt tcggtgttga ctctcaaaat atcggacaac
     7441 aaaatggcat cggacaagtt cttagcagcc aaaaacaaat cagcgtattg atcaccgatt
     7501 tgagccaaca agttatccaa atcgtcatcg taggtatcct tagataattg caacttggca
     7561 tcttcagcca aatcgaaatt ggacttgaag tttggagtca aacccaaaga caaggcaatc
     7621 aaattaccga acaaaccatt cttcttttca cctggtaatt gggcgatcaa attttctaat
     7681 cttctggact tggacaatct ggcagacaaa atagctttag catcaacacc agaggcgttg
     7741 attgggtttt cttcgaataa ttgattgtag gtttggacta attggatgaa caacttatca
     7801 acatcggagt tatctgggtt caaatcacct tcgatcaaga aatgacctct gaacttaatc
     7861 atgtgggcca aagccaaata gatcaatctc aaatcagcct tatcggtaga gtcaaccaac
     7921 ttctttctca aatggtagat agttgggtac ttttcgtggt aagcaacttc atcaacgatg
     7981 ttaccgaaga ttggatgtct ttcgtgcttc ttatcttctt cgaccaagaa ggattcttcc
     8041 aatctgtgga agaatgaatc atcaaccttg gccatttcgt tggagaagat ttcttgcaag
     8101 tagcagattc tgttctttct tctggtgtat cttcttctag cggttctttt caatctagta
     8161 gcttcagcag tttcaccaga atcgaacaac aaggcaccaa tcaagttctt tttgatggag
     8221 tgtctatcag tgttacccaa aaccttgaac ttcttggatg gaaccttgta ttcatcagta
     8281 ataacagccc aaccaacaga gtttgtaccg atagccaaac caatagagta tttcttatcc
     8341 ccgggtctaa cttttctctt cttctttggg gctctagaca ttcttttaat cgtggatcct
     8401 gttaattcag taaattttcg atcttgggaa gaaaaaagca gtaagcgtga aaaatctaaa
     8461 agctgatgta gtagaagatc ctattcttta acaaagattg accttttctt tttcttcttg
     8521 gtttgagtag aaaggggaag gaagaataca agagagagga aaaaaaggaa gataaaaaga
     8581 gagcgtgata taaatgaata tatattaaac aagagagatt gggaaggaaa ggatcaaaca
     8641 aacccaaaaa tatttcaaaa aggagagaga gaggcgagtt tggtttcaaa acggtttatt
     8701 tatttatgca agaggacgtg gaagaaaaag aagaaggaag aaaaaaattt gaaagaaaaa
     8761 aacgcgtggc gggtaaagaa gaaaatggaa aatagaggcc gggtgacaga gaaatattga
     8821 gggttaattg gaaaatatgt tagggtgagg catatgtttt taagggtttt gaggatccga
     8881 taaggaagaa tgtaggttaa atgttgtgca ttaattgctg tggcagctta cccgcttccc
     8941 cacacatttt ggtagtatct gtcctcttgt tattgttact gtaattgtgt atatatgttc
     9001 tcgcgtgtgt cttatttact tatttagtta ttatattata tgggtctgca aggtagaggc
     9061 ggcgcgtggc ggccgctcac ttgtacagct cgtccatgcc gccggtggag tggcggccct
     9121 cggcgcgttc gtactgttcc acgatggtgt agtcctcgtt gtgggaggtg atgtccaact
     9181 tgatgttgac gttgtaggcg ccgggcagct gcacgggctt cttggccttg taggtggtct
     9241 tgacctcagc gtcgtagtgg ccgccgtcct tcagcttcag cctctgcttg atctcgccct
     9301 tcagggcgcc gtcctcgggg tacatccgct cggaggaggc ctcccagccc atggtcttct
     9361 tctgcattac ggggccgtcg gaggggaagt tggtgccgcg cagcttcacc ttgtagatga
     9421 actcgccgtc ctgcagggag gagtcctggg tcacggtcac cacgccgccg tcctcgaagt
     9481 tcatcacgcg ctcccacttg aagccctcgg ggaaggacag cttcaagtag tcggggatgt
     9541 cggcggggtg cttcacgtag gccttggagc cgtacatgaa ctgaggggac aggatgtccc
     9601 aggcgaaggg cagggggcca cccttggtca ccttcagctt ggcggtctgg gtgccctcgt
     9661 aggggcggcc ctcgccctcg ccctcgatct cgaactcgtg gccgttcacg gagccctcca
     9721 tgtgcacctt gaagcgcatg aactccttga tgatggccat gttatcctcc tcgcccttgc
     9781 tcaccatcat tttaatatct agattagtgt gtgtatttgt gtttgtgtgt ctatagaagt
     9841 atagtaattt atgctgcaaa ggtcctaatg tataaggaaa gaatatttag agaaaagaag
     9901 aaaacaagag ttttatatac atacagagca catgcatgcc ataggatcct actgtatgta
     9961 catacagtac ccgggctgca ggaattcgat ggatccaacc atgatgagta tagtccctta
    10021 cattgatggc ttaaacagca ttgccaaaat ttctaaacta agcaacagtg atcctggcct
    10081 agtgatagag tgcatacgtc acttaatata ctataagtgt gttacattat cagatatttt
    10141 ccaattttct aacatatacg ctccttcttc attaattagg aattttttga ctgatccact
    10201 aatggctagt gactgtcaat cttatgttac gttcccagaa gtgtctaaaa tatcgaactt
    10261 gcccttaaac aaaagtctgg gttcaggtga ccaagactca ccatcatttt ctgtacgaag
    10321 aaaatccaaa tcatctagca taccgtcaaa cccggattct agaactacat cttttagttc
    10381 taccagtaga gtttctcaaa actcctcctt aaattcctct ttttcatcaa tttataaaga
    10441 ctggaggcaa tcacaaacct cctgctcgag ttcaaatatt catgtcatca acaatcgtaa
    10501 ccgattccta ccaacaagat catgcctatt tgacctttat aggtcacttt cacaaggtca
    10561 aacgctgaaa acatggtatg agtcgaaata tatgatttta aaagaaaata acattgatat
    10621 aagaaggttc ataacgtttg gcctggaaaa acgtatcatt tacaggtgct actcttttcc
    10681 tgtaatgata aatgcaggtt cacgtgaacc aaaagaaatg acaccaataa taacaaaaga
    10741 tttagtgaat aatgataagt tacttgaaaa aagaaatcac aatcatttgc tttcagccac
    10801 agggtctaga aatacggcac aatccggcaa cttgaaacca gaaagaccat caaaggtttc
    10861 atttgaaatg caaagggtaa gttcgttggc aacaggcaaa agtactatgc cgaaactcag
    10921 cgacgaagaa gaaggtattc ttgaagagtc tattaggaat gccgaaacat ttgataaaat
    10981 ttgcgtttta ctgagcaagc ctaaattaga agtcgaaagc tacctaaacg aattaggaga
    11041 atttaaatgc taagctattt ccaagggttt gtgcccatac acacaatatt ttactccgtg
    11101 ttccatccta cagaaggttc taagattaaa tacgagtttc cgccaaataa tttgaaaaat
    11161 catggtatta atttcaacac atttaaaaac tacatcatac cgaagcctat attatgccac
    11221 aagttgatca cttttaagta tggtacatat aggattgtat gttatcctgt gacaatcaat
    11281 tctccaatat atgctaggaa tttttttagt tttaattttg tatttgtatt cccatatgat
    11341 tgtgagacgt ctccctacga accagctatt actaggctgg gcaaaatgtt caaagttctt
    11401 gaagaacaaa atcaattgct atccaaatcg gaaagagatc cagttttctt cgatttgaag
    11461 gtgttggaaa actccacaac aacaccctcc actgcaggtc cttcatccac gccaaatcct
    11521 agtagtaaca ccacaccaac tcatcctaca tccgaaaagg atacaaagga tatgagaagc
    11581 agcagataca gtgaccttat caaggatttg ggccttccgc aatccgcatt ttccatacaa
    11641 gatttattga tgagaatttt ccaagacctg aataactatt ccgaatgcct cataccaatt
    11701 gatgagggaa atgcagtaga cataaagatt tttccgctgt tgagaccacc gacaacttgt
    11761 gtctcattag aagatgtgcc cctgtcgtct gtaaatttg
//
