LOCUS       sequence-304484-       15759 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Baculovirus transfer vector for expression of huntingtin protein in 
            insect and mammalian cellscells as well as in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15759)
  AUTHORS   Harding RJ, Loppnau P, Ackloo S, Lemak A, Hutchinson A, Hunt B, 
            Holehouse AS, Ho JC, Fan L, Toledo-Sherman L, Seitova A, Arrowsmith 
            CH
  TITLE     Design and characterization of mutant and wildtype huntingtin 
            proteins produced from a toolkit of scalable eukaryotic expression 
            systems.
  JOURNAL   J Biol Chem. 2019 Apr 26;294(17):6986-7001. doi: 
            10.1074/jbc.RA118.007204. Epub 2019 Mar 6.
  PUBMED    30842263
REFERENCE   2  (bases 1 to 15759)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.RA118.007204"; journalName: "J Biol Chem"; date: 
            "2019-04-26- 26"; volume: "294"; issue: "17"; pages: "6986-7001"
COMMENT     Sequence Label: pBacMam2-DiEx-LIC-N-flag_huntingtin_full-lengt...
FEATURES             Location/Qualifiers
     source          1..15759
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      121..575
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(208..227)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     417..438
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        601..705
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(706..774,775..1566)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(924..943)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1737..2325
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2226..2245
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2479..2496
                     /label=L4440
                     /note="L4440 vector, forward primer"
     mobile_element  2630..2854
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7R
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
     CDS             complement(2921..3454)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     promoter        complement(join(3643..3648,3649..3665,3666..3671))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     polyA_signal    4179..4227
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     primer_bind     4252..4271
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     enhancer        4332..4711
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        4712..4915
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     4866..4886
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     promoter        4962..5071
                     /label=p10 promoter
                     /note="baculovirus promoter for expression in insect cells"
     CDS             5106..5129
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     regulatory      13867..13876
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             14571..14600
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
     CDS             14601..14624
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     primer_bind     complement(14714..14734)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(15132..15143)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    15368..15502
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(15405..15424)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     15459..15478
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     mobile_element  15531..15696
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
ORIGIN
        1 cgtcatcttc ggctactttt tctctgtcac agaatgaaaa tttttctgtc atctcttcgt
       61 tattaatgtt tgtaattgac tgaatatcaa cgcttatttg cagcctgaat ggcgaatggg
      121 acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg
      181 ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca
      241 cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta
      301 gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc
      361 catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc ttaatagtgg
      421 actcttgttc caaactggaa caacactcaa ccctatctcg gtctattctt ttgatttata
      481 agggattttg ccgatttcgg cctattggtt aaaaaatgag ctgatttaac aaaaatttaa
      541 cgcgaatttt aacaaaatat taacgcttac aatttaggtg gcacttttcg gggaaatgtg
      601 cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga
      661 caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat
      721 ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca
      781 gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc
      841 gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca
      901 atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtat tgacgccggg
      961 caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca
     1021 gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata
     1081 accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag
     1141 ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg
     1201 gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca
     1261 acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta
     1321 atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct
     1381 ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca
     1441 gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag
     1501 gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat
     1561 tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt
     1621 taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa
     1681 cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga
     1741 gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg
     1801 gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc
     1861 agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag
     1921 aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc
     1981 agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg
     2041 cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac
     2101 accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga
     2161 aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt
     2221 ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag
     2281 cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg
     2341 gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta
     2401 tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc
     2461 agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg
     2521 tattttctcc ttacgcatct gtgcggtatt tcacaccgca tagaccagcc gcgtaacctg
     2581 gcaaaatcgg ttacggttga gtaataaatg gatgccctgc gtaagcgggt gtgggcggac
     2641 aataaagtct taaactgaac aaaatagatc taaactatga caataaagtc ttaaactaga
     2701 cagaatagtt gtaaactgaa atcagtccag ttatgctgtg aaaaagcata ctggactttt
     2761 gttatggcta aagcaaactc ttcattttct gaagtgcaaa ttgcccgtcg tattaaagag
     2821 gggcgtggcc aagggcatgg taaagactat attcgcggcg ttgtgacaat ttaccgaaca
     2881 actccgcggc cgggaagccg atctcggctt gaacgaattg ttaggtggcg gtacttgggt
     2941 cgatatcaaa gtgcatcact tcttcccgta tgcccaactt tgtatagaga gccactgcgg
     3001 gatcgtcacc gtaatctgct tgcacgtaga tcacataagc accaagcgcg ttggcctcat
     3061 gcttcaagag attgatgagc gcggtggcaa tgccctgcct ccggtgctcg ccggagactg
     3121 cgagatcata gatatagatc tcactacgcg gctgctcaaa cttgggcaga acgtaagccg
     3181 cgagagcgcc aacaaccgct tcttggtcga aggcagcaag cgcgatgaat gtcttactac
     3241 ggagcaagtt cccgaggtaa tcggagtccg gctgatgttg ggagtaggtg gctacgtctc
     3301 cgaactcacg accgaaaaga tcaagagcag cccgcatgga tttgacttgg tcagggccga
     3361 gcctacatgt gcgaatgatg cccatacttg agccacctaa ctttgtttta gggcgactgc
     3421 cctgctgcgt aacatcgttg ctgctgcgta acatcgttgc tgctccataa catcaaacat
     3481 cgacccacgg cgtaacgcgc ttgctgcttg gatgcccgag gcatagactg tacaaaaaaa
     3541 cagtcataac aagccatgaa aaccgccact gcgccgttac caccgctgcg ttcggtcaag
     3601 gttctggacc agttgcgtga gcgcatacgc tacttgcatt acagtttacg aaccgaacag
     3661 gcttatgtca actgggttcg tgccttcatc cgtttccacg gtgtgcgtca cccggcaacc
     3721 ttgggcagca gcgaagtcga ggcatttctg tcctggctgg cgaacgagcg caaggtttcg
     3781 gtctccacgc atcgtcaggc attggcggcc ttgctgttct tctacggcaa ggtgctgtgc
     3841 acggatctgc ccttgcttca ggagatcggt agacctcggc cgtcgcggcg cttgccggtg
     3901 gtgctgaccc cggatgaagt ggttcgcatc ctcggttttc tggaaggcga gcatcgtttg
     3961 ttcgcccagg actctagcta tagttctagt ggttggctac gtacccgtag tggctatggc
     4021 agggcttgcc gccccgacgt tggctgcgag ccctgggcct tcacccgaac ttgggggttg
     4081 gggtggggaa aaggaagaaa cgcgggcgta ttggtcccaa tggggtctcg gtggggtatc
     4141 gacagagtgc cagccctggg accgaacccc gcgtttatga acaaacgacc caacacccgt
     4201 gcgttttatt ctgtcttttt attgccgtca tagcgcgggt tccttccggt attgtctcct
     4261 tccgtgtttc agttagcctc ccccatctcc cggtaccgca tgcggatctg tacgggccag
     4321 atatacgcgt tgacattgat tattgactag ttattaatag taatcaatta cggggtcatt
     4381 agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg gcccgcctgg
     4441 ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc ccatagtaac
     4501 gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt
     4561 ggcagtacat caagtgtatc atatgccaag tacgccccct attgacgtca atgacggtaa
     4621 atggcccgcc tggcattatg cccagtacat gaccttatgg gactttccta cttggcagta
     4681 catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt acatcaatgg
     4741 gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg acgtcaatgg
     4801 gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca actccgcccc
     4861 attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca gagctctctg
     4921 gctaactaga gaacccactg cttactggct tatcgaatac ggacctttaa ttcaacccaa
     4981 cacaatatat tatagttaaa taagaattat tatcaaatca tttgtatatt aattaaaata
     5041 ctatactgta aattacattt tatttacaat cactcgacgg atccttaaga aggagatata
     5101 ctatggacta caaagacgat gacgacaaga tggcgaccct ggaaaagctg atgaaggcct
     5161 tcgagtccct caagtccttc caacagcagc aacagcaaca acagcagcaa cagcaacaac
     5221 agcagcaaca gcaacaaccg ccaccgccgc cgccgccgcc gccgcctcct cagcttcctc
     5281 agccgccgcc gcaggcacag ccgctgctgc ctcagccgca gccgcccccg ccgccgcccc
     5341 cgccgccacc cggcccggct gtggctgagg agccgctgca ccgaccaaag aaagaacttt
     5401 cagctaccaa gaaagaccgt gtgaatcatt gtctgacaat atgtgaaaac atagtggcac
     5461 agtctgtcag aaattctcca gaatttcaga aacttctggg catcgctatg gaactttttc
     5521 tgctgtgcag tgatgacgca gagtcagatg tcaggatggt ggctgacgaa tgcctcaaca
     5581 aagttatcaa agctttgatg gattctaatc ttccaaggtt acagctcgag ctctataagg
     5641 aaattaaaaa gaatggtgcc cctcggagtt tgcgtgctgc cctgtggagg tttgctgagc
     5701 tggctcacct ggttcggcct cagaaatgca ggccttacct ggtgaacctt ctgccgtgcc
     5761 tgactcgaac aagcaagaga cccgaagaat cagtccagga gaccttggct gcagctgttc
     5821 ccaaaattat ggcttctttt ggcaattttg caaatgacaa tgaaattaag gttttgttaa
     5881 aggccttcat agcgaacctg aagtcaagct cccccaccat tcggcggaca gcggctggat
     5941 cagcagtgag catctgccag cactcaagaa ggacacaata tttctatagt tggctactaa
     6001 atgtgctctt aggcttactc gttcctgtcg aggatgaaca ctccactctg ctgattcttg
     6061 gcgtgctgct caccctgagg tatttggtgc ccttgctgca gcagcaggtc aaggacacaa
     6121 gcctgaaagg cagcttcgga gtgacaagga aagaaatgga agtctctcct tctgcagagc
     6181 agcttgtcca ggtttatgaa ctgacgttac atcatacaca gcaccaagac cacaatgttg
     6241 tgaccggagc cctggagctg ttgcagcagc tcttcagaac gcctccaccc gagcttctgc
     6301 aaaccctgac cgcagtcggg ggcattgggc agctcaccgc tgctaaggag gagtctggtg
     6361 gccgaagccg tagtgggagt attgtggaac ttatagctgg agggggttcc tcatgcagcc
     6421 ctgtcctttc aagaaaacaa aaaggcaaag tgctcttagg agaagaagaa gccttggagg
     6481 atgactctga atcgagatcg gatgtcagca gctctgcctt aacagcctca gtgaaggatg
     6541 agatcagtgg agagctggct gcttcttcag gggtttccac tccagggtca gcaggtcatg
     6601 acatcatcac agaacagcca cggtcacagc acacactgca ggcggactca gtggatctgg
     6661 ccagctgtga cttgacaagc tctgccactg atggggatga ggaggatatc ttgagccaca
     6721 gctccagcca ggtcagcgcc gtcccatctg accctgccat ggacctgaat gatgggaccc
     6781 aggcctcgtc gcccatcagc gacagctccc agaccaccac cgaagggcct gattcagctg
     6841 ttaccccttc agacagttct gaaattgtgt tagacggtac cgacaaccag tatttgggcc
     6901 tgcagattgg acagccccag gatgaagatg aggaagccac aggtattctt cctgatgaag
     6961 cctcggaggc cttcaggaac tcttccatgg cccttcaaca ggcacattta ttgaaaaaca
     7021 tgagtcactg caggcagcct tctgacagca gtgttgataa atttgtgttg agagatgaag
     7081 ctactgaacc gggtgatcaa gaaaacaagc cttgccgcat caaaggtgac attggacagt
     7141 ccactgatga tgactctgca cctcttgtcc attgtgtccg ccttttatct gcttcgtttt
     7201 tgctaacagg gggaaaaaat gtgctggttc cggacaggga tgtgagggtc agcgtgaagg
     7261 ccctggccct cagctgtgtg ggagcagctg tggccctcca cccggaatct ttcttcagca
     7321 aactctataa agttcctctt gacaccacgg aataccctga ggaacagtat gtctcagaca
     7381 tcttgaacta catcgatcat ggagacccac aggttcgagg agccactgcc attctctgtg
     7441 ggaccctcat ctgctccatc ctcagcaggt cccgcttcca cgtgggagat tggatgggca
     7501 ccattagaac cctcacagga aatacatttt ctttggcgga ttgcattcct ttgctgcgga
     7561 aaacactgaa ggatgagtct tctgttactt gcaagttagc ttgtacagct gtgaggaact
     7621 gtgtcatgag tctctgcagc agcagctaca gtgagttagg actgcagctg atcatcgatg
     7681 tgctgactct gaggaacagt tcctattggc tggtgaggac agagcttctg gaaacccttg
     7741 cagagattga cttcaggctg gtgagctttt tggaggcaaa agcagaaaac ttacacagag
     7801 gggctcatca ttatacaggg cttttaaaac tgcaagaacg agtgctcaat aatgttgtca
     7861 tccatttgct tggagatgaa gaccccaggg tgcgacatgt tgccgcagca tcactaatta
     7921 ggcttgtccc aaagctgttt tataaatgtg accaaggaca agctgatcca gtagtggccg
     7981 tggcaagaga tcaaagcagt gtttacctga aacttctcat gcatgagacg cagcctccat
     8041 ctcatttctc cgtcagcaca ataaccagaa tatatagagg ctataaccta ctaccaagca
     8101 taacagacgt cactatggaa aataaccttt caagagttat tgcagcagtt tctcatgaac
     8161 taatcacatc aaccaccaga gcactcacat ttggatgctg tgaagctttg tgtcttcttt
     8221 ccactgcctt cccagtttgc atttggagtt taggttggca ctgtggagtg cctccactga
     8281 gtgcctcaga tgagtctagg aagagctgta ccgttgggat ggccacaatg attctgaccc
     8341 tgctctcgtc agcttggttc ccattggatc tctcagccca tcaagatgct ttgattttgg
     8401 ccggaaactt gcttgcagcc agtgctccca aatctctgag aagttcatgg gcctctgaag
     8461 aagaagccaa cccagcagcc accaagcaag aggaggtctg gccagccctg ggggaccggg
     8521 ccctggtgcc catggtggag cagctcttct ctcacctgct gaaggtgatt aacatttgtg
     8581 cccacgtcct ggatgacgtg gctcctggac ccgcaataaa ggcagccttg ccttctctaa
     8641 caaacccccc ttctctaagt cccatccgac gaaaggggaa ggagaaagaa ccaggagaac
     8701 aagcatctgt accgttgagt cccaagaaag gcagtgaggc cagtgcagct tctagacaat
     8761 ctgatacctc aggtcctgtt acaacaagta aatcctcatc actggggagt ttctatcatc
     8821 ttccttcata cctcaaactg catgatgtcc tgaaagctac acacgctaac tacaaggtca
     8881 cgctggatct tcagaacagc acggaaaagt ttggagggtt tcttcgctca gccttggatg
     8941 ttctttctca gatactagag ctggccacac tgcaggacat tgggaagtgt gttgaagaga
     9001 tcctaggata cctgaaatcc tgctttagtc gagaaccaat gatggcaact gtttgtgttc
     9061 aacaattgtt gaagactctc tttggcacaa acttggcctc ccagtttgat ggcttatctt
     9121 ccaaccccag caagtcacaa ggccgagcac agcgccttgg ctcctccagt gtgaggccag
     9181 gcttgtacca ctactgcttc atggccccgt acacccactt cacccaggcc ctcgctgacg
     9241 ccagcctgag gaacatggtg caggcggagc aggagaacga cacctcggga tggtttgatg
     9301 tcctccagaa agtgtctacc cagttgaaga caaacctcac gagtgtcaca aagaaccgtg
     9361 cagataagaa tgctattcat aatcacattc gtttgtttga acctcttgtt ataaaagctt
     9421 taaaacagta cacgactaca acatgtgtgc agttacagaa gcaggtttta gatttgctgg
     9481 cgcagctggt tcagttacgg gttaattact gtcttctgga ttcagatcag gtgtttattg
     9541 gctttgtatt gaaacagttt gaatacattg aagtgggcca gttcagggaa tcagaggcaa
     9601 tcattccaaa catctttttc ttcttggtat tactatctta tgaacgctat cattcaaaac
     9661 agatcattgg aattcctaaa atcattcagc tctgtgatgg catcatggcc agtggaagga
     9721 aggctgtgac acatgccata ccggctctgc agcccatagt ccacgacctc tttgtattaa
     9781 gaggaacaaa taaagctgat gcaggaaaag agcttgaaac ccaaaaagag gtggtggtgt
     9841 caatgttact gagactcatc cagtaccatc aggtgttgga gatgttcatt cttgtcctgc
     9901 agcagtgcca caaggagaat gaagacaagt ggaagcgact gtctcgacag atagctgaca
     9961 tcatcctccc aatgttagcc aaacagcaga tgcacattga ctctcatgaa gcccttggag
    10021 tgttaaatac attatttgag attttggccc cttcctccct ccgtccggta gacatgcttt
    10081 tacggagtat gttcgtcact ccaaacacaa tggcgtccgt gagcactgtt caactgtgga
    10141 tatcgggaat tctggccatt ttgagggttc tgatttccca gtcaactgaa gatattgttc
    10201 tttctcgtat tcaggagctc tccttctctc cgtatttaat ctcctgtaca gtaattaata
    10261 ggttaagaga tggggacagt acttcaacgc tagaagaaca cagtgaaggg aaacaaataa
    10321 agaatttgcc agaagaaaca ttttcaaggt ttctattaca actggttggt attcttttag
    10381 aagacattgt tacaaaacag ctgaaggtgg aaatgagtga gcagcaacat actttctatt
    10441 gccaggaact aggcacactg ctaatgtgtc tgatccacat cttcaagtct ggaatgttcc
    10501 ggagaatcac agcagctgcc actaggctgt tccgcagtga tggctgtggc ggcagtttct
    10561 acaccctgga cagcttgaac ttgcgggctc gttccatgat caccacccac ccggccctgg
    10621 tgctgctctg gtgtcagata ctgctgcttg tcaaccacac cgactaccgc tggtgggcag
    10681 aagtgcagca gaccccgaaa agacacagtc tgtccagcac aaagttactt agtccccaga
    10741 tgtctggaga agaggaggat tctgacttgg cagccaaact tggaatgtgc aatagagaaa
    10801 tagtacgaag aggggctctc attctcttct gtgattatgt ctgtcagaac ctccatgact
    10861 ccgagcactt aacgtggctc attgtaaatc acattcaaga tctgatcagc ctttcccacg
    10921 agcctccagt acaggacttc atcagtgccg ttcatcggaa ctctgctgcc agcggcctgt
    10981 tcatccaggc aattcagtct cgttgtgaaa acctttcaac tccaaccatg ctgaagaaaa
    11041 ctcttcagtg cttggagggg atccatctca gccagtcggg agctgtgctc acgctgtatg
    11101 tggacaggct tctgtgcacc cctttccgtg tgctggctcg catggtcgac atccttgctt
    11161 gtcgccgggt agaaatgctt ctggctgcaa atttacagag cagcatggcc cagttgccaa
    11221 tggaagaact caacagaatc caggaatacc ttcagagcag cgggctcgct cagagacacc
    11281 aaaggctcta ttccctgctg gacaggtttc gtctctccac catgcaagac tcacttagtc
    11341 cctctcctcc agtctcttcc cacccgctgg acggggatgg gcacgtgtca ctggaaacag
    11401 tgagtccgga caaagactgg tacgttcatc ttgtcaaatc ccagtgttgg accaggtcag
    11461 attctgcact gctggaaggt gcagagctgg tgaatcggat tcctgctgaa gatatgaatg
    11521 ccttcatgat gaactcggag ttcaacctaa gcctgctagc tccatgctta agcctaggga
    11581 tgagtgaaat ttctggtggc cagaagagtg ccctttttga agcagcccgt gaggtgactc
    11641 tggcccgtgt gagcggcacc gtgcagcagc tccctgctgt ccatcatgtc ttccagcccg
    11701 agctgcctgc agaaccggcg gcctactgga gcaagttgaa tgatctgttt ggggatgctg
    11761 cactgtatca gtccctgccc actctggccc gggccctggc acagtacctg gtggtggtct
    11821 ccaaactgcc cagtcatttg caccttcctc ctgagaaaga gaaggacatt gtgaaattcg
    11881 tggtggcaac ccttgaggcc ctgtcctggc atttgatcca tgagcagatc ccgctgagtc
    11941 tggatctcca ggcagggctg gactgctgct gcctggccct gcagctgcct ggcctctgga
    12001 gcgtggtctc ctccacagag tttgtgaccc acgcctgctc cctcatccac tgtgtgcact
    12061 tcatcctgga ggccgttgca gtgcagcctg gagagcagct tcttagtcca gaaagaagga
    12121 caaatacccc aaaagccatc agcgaggagg aggaggaagt agatccaaac acacagaatc
    12181 ctaagtatat cactgcagcc tgtgagatgg tggcagaaat ggtggagtct ctgcagtcgg
    12241 tgttggcctt gggtcataaa aggaatagcg gcgtgccggc gtttctcacg ccattgctca
    12301 ggaacatcat catcagcctg gcccgcctgc cccttgtcaa cagctacaca cgtgtgcccc
    12361 cactggtgtg gaagcttgga tggtcaccca aaccgggagg ggattttggc acagcattcc
    12421 ctgagatccc cgtggagttc ctccaggaaa aggaagtctt taaggagttc atctaccgca
    12481 tcaacacact aggctggacc agtcgtactc agtttgaaga aacttgggcc accctccttg
    12541 gtgtcctggt gacgcagccc ctcgtgatgg agcaggagga gagcccacca gaagaagaca
    12601 cagagaggac ccagatcaac gtcctggccg tgcaggccat cacctcactg gtgctcagtg
    12661 caatgactgt gcctgtggcc ggcaacccag ctgtaagctg cttggagcag cagccccgga
    12721 acaagcctct gaaagctctc gacaccaggt ttgggaggaa gctgagcatt atcagaggga
    12781 ttgtggagca agagattcaa gcaatggttt caaagagaga gaatattgcc acccatcatt
    12841 tatatcaggc atgggatcct gtcccttctc tgtctccggc tactacaggt gccctcatca
    12901 gccacgagaa gctgctgctg cagatcaacc ccgagcggga gctggggagc atgagctaca
    12961 aactcggcca ggtgtccata cactccgtgt ggctagggaa cagcatcaca cccctgaggg
    13021 aggaggaatg ggacgaggaa gaggaggagg aggccgacgc ccctgcacct tcgtcaccac
    13081 ccacgtctcc agtcaactcc aggaaacacc gggctggagt tgacatccac tcctgttcgc
    13141 agtttttgct tgagttgtac agccgctgga tcctgccgtc cagctcagcc aggaggaccc
    13201 cggccatcct gatcagtgag gtggtcagat cccttctagt ggtctcagac ttgttcaccg
    13261 agcgcaacca gtttgagctg atgtatgtga cgctgacaga actgcgaagg gtgcaccctt
    13321 cagaagacga gatcctcgct cagtacctgg tgcctgccac ctgcaaggca gctgccgtcc
    13381 ttgggatgga caaggccgtg gcggagcctg tcagccgcct gctggagagc acgctcagga
    13441 gcagccacct gcccagcagg gttggagccc tgcacggcat cctctatgtg ctggagtgcg
    13501 acctgctgga cgacactgcc aagcagctca tcccggtcat cagcgactat ctcctctcca
    13561 acctgaaagg gatcgcccac tgcgtgaaca ttcacagcca gcagcacgta ctggtcatgt
    13621 gtgccactgc gttttacctc attgagaact atcctctgga cgtagggccg gaattttcag
    13681 catcaataat acagatgtgt ggggtgatgc tgtctggaag tgaggagtcc accccctcca
    13741 tcatttacca ctgtgccctc agaggcctgg agcgcctcct gctctctgag cagctctccc
    13801 gcctggatgc agaatcgctg gtcaagctga gtgtggacag agtgaacgtg cacagcccgc
    13861 accgggccat ggcggctctg ggcctgatgc tcacctgcat gtacacagga aaggagaaag
    13921 tcagtccggg tagaacttca gaccctaatc ctgcagcccc cgacagcgag tcagtgattg
    13981 ttgctatgga gcgggtatct gttctttttg ataggatcag gaaaggcttt ccttgtgaag
    14041 ccagagtggt ggccaggatc ctgccccagt ttctagacga cttcttccca ccccaggaca
    14101 tcatgaacaa agtcatcgga gagtttctgt ccaaccagca gccatacccc cagttcatgg
    14161 ccaccgtggt gtataaggtg tttcagactc tgcacagcac cgggcagtcg tccatggtcc
    14221 gggactgggt catgctgtcc ctctccaact tcacgcagag ggccccggtc gccatggcca
    14281 cgtggagcct ctcctgcttc tttgtcagcg cgtccaccag cccgtgggtc gcggcgatcc
    14341 tcccacatgt catcagcagg atgggcaagc tggagcaggt ggacgtgaac cttttctgcc
    14401 tggtcgccac agacttctac agacaccaga tagaggagga gctcgaccgc agggccttcc
    14461 agtctgtgct tgaggtggtt gcagccccag gaagcccata tcaccggctg ctgacttgtt
    14521 tacgaaatgt ccacaaggtc accacctgct aaaacctcta cttccaatcg caccatcatc
    14581 accatcacca tcaccaccat gattacaagg atgacgacga taagtgaggc ggccgccaac
    14641 ttgatatcaa gcttatcgat aatcaacctc tggattacaa aatttgtgaa agattgactg
    14701 gtattcttaa ctatgttgct ccttttacgc tatgtggata cgctgcttta atgcctttgt
    14761 atcatgctat tgcttcccgt atggctttca ttttctcctc cttgtataaa tcctggttgc
    14821 tgtctcttta tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt
    14881 ttgctgacgc aacccccact ggttggggca ttgccaccac ctgtcagctc ctttccggga
    14941 ctttcgcttt ccccctccct attgccacgg cggaactcat cgccgcctgc cttgcccgct
    15001 gctggacagg ggctcggctg ttgggcactg acaattccgt ggtgttgtcg gggaaatcat
    15061 cgtcctttcc ttggctgctc gcctgtgttg ccacctggat tctgcgcggg acgtccttct
    15121 gctacgtccc ttcggccctc aatccagcgg accttccttc ccgcgggcgc ccccctcctc
    15181 acggcgagcg aagcttgtcg agaagtacta gaggatctct agagcctgca gtctcgacaa
    15241 gcttgtcgag aagtactaga ggatcataat cagccatacc acatttgtag aggttttact
    15301 tgctttaaaa aacctcccac acctccccct gaacctgaaa cataaaatga atgcaattgt
    15361 tgttgttaac ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa
    15421 tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa
    15481 tgtatcttat catgtctgga tctgatcact gcttgagcct aggagatccg aaccagataa
    15541 gtgaaatcta gttccaaact attttgtcat ttttaatttt cgtattagct tacgacgcta
    15601 cacccagttc ccatctattt tgtcactctt ccctaaataa tccttaaaaa ctccatttcc
    15661 acccctccca gttcccaact attttgtccg cccacagcgg ggcatttttc ttcctgttat
    15721 gtttttaatc aaacatcctg ccaactccat gtgacaaac
//
