LOCUS       sequence-304498-       13777 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  express CARPID dCasRx-BASU fusion protein in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13777)
  AUTHORS   Yi W, Li J, Zhu X, Wang X, Fan L, Sun W, Liao L, Zhang J, Li X, Ye 
            J, Chen F, Taipale J, Chan KM, Zhang L, Yan J
  TITLE     CRISPR-assisted detection of RNA-protein interactions in living 
            cells.
  JOURNAL   Nat Methods. 2020 Jun 22. pii: 10.1038/s41592-020-0866-0. doi: 
            10.1038/s41592-020-0866-0.
  PUBMED    32572232
REFERENCE   2  (bases 1 to 13777)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2020 Jun 22. pii: 10.1038/s41592-020-0866-0. doi: 
            10.1038/s41592-020-0866-0."
COMMENT     Sequence Label: CARPID dCasRx-BASU
FEATURES             Location/Qualifiers
     source          1..13777
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..181
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    228..353
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    846..1079
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1264..1308
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1606..1723
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        1848..3026
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          2079..3017
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     2974..2994
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             3045..3065
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             3075..5972
                     /codon_start=1
                     /product="RNA-targeting Type VI CRISPR protein from 
                     Ruminococcus flavefaciens strain XPD3002 (Konermann et al.,
                     2018)"
                     /label=RfxCas13d
                     /translation="IEKKKSFAKGMGVKSTLVSGSKVYMTTFAEGSDARLEKIVEGDSI
                     RSVNEGEAFSAEMADKNAGYKIGNAKFSHPKGYAVVANNPLYTGPVQQDMLGLKETLEK
                     RYFGESADGNDNICIQVIHNILDIEKILAEYITNAAYAVNNISGLDKDIIGFGKFSTVY
                     TYDEFKDPEHHRAAFNNNDKLINAIKAQYDEFDNFLDNPRLGYFGQAFFSKEGRNYIIN
                     YGNECYDILALLSGLAHWVVANNEEESRISRTWLYNLDKNLDNEYISTLNYLYDRITNE
                     LTNSFSKNSAANVNYIAETLGINPAEFAEQYFRFSIMKEQKNLGFNITKLREVMLDRKD
                     MSEIRKNHKVFDSIRTKVYTMMDFVIYRYYIEEDAKVAAANKSLPDNEKSLSEKDIFVI
                     NLRGSFNDDQKDALYYDEANRIWRKLENIMHNIKEFRGNKTREYKKKDAPRLPRILPAG
                     RDVSAFSKLMYALTMFLDGKEINDLLTTLINKFDNIQSFLKVMPLIGVNAKFVEEYAFF
                     KDSAKIADELRLIKSFARMGEPIADARRAMYIDAIRILGTNLSYDELKALADTFSLDEN
                     GNKLKKGKHGMRNFIINNVISNKRFHYLIRYGDPAHLHEIAKNEAVVKFVLGRIADIQK
                     KQGQNGKNQIDRYYETCIGKDKGKSVSEKVDALTKIITGMNYDQFDKKRSVIEDTGREN
                     AEREKFKKIISLYLTVIYHILKNIVNINARYVIGFHCVERDAQLYKEKGYDINLKKLEE
                     KGFSSVTKLCAGIDETAPDKRKDVEKEMAERAKESIDSLESANPKLYANYIKYSDEKKA
                     EEFTRQINREKAKTALNAYLRNTKWNVIIREDLLRIDNKTCTLFANKAVALEVARYVHA
                     YINDIAEVNSYFQLYHYIMQRIIMNERYEKSSGKVSEYFDAVNDEKKYNDRLLKLLCVP
                     FGYCIPRFKNLSIEALFDRNEAAKFDKEKKKVSGNS"
     CDS             5982..6002
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             6012..6038
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             6840..6893
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             join(6894..6896,6897..7610)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     CDS             join(6894..6896,6897..7607)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(6936..6957)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(7197..7216)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     7544..7565
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    7635..8223
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7688..7708)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8106..8117)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(8226..8242)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8227..8243)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             8748..8928
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(8954..8971)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    8960..9184
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      9230..9658
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9317..9336)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9527..9548
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(9667..9687)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        9672..10001
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      9852..9987
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     9914..9933
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        10049..10096
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             10115..10489
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    10619..10740
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10656..10675)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10710..10729
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(10789..10805)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10789..10805)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(10802..10824)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    10813..10829
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(10837..10843,10844..10861,10862..10867))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10882..10903
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(11020..11037)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(11191..11779)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(11271..11290)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(11950..12741,12742..12810))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     12573..12592
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(12811..12915)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(12990..13009)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        13181..13560
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        13562..13760
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     13715..13735
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
ORIGIN
        1 gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca
       61 ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg
      121 tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc
      181 agtggcgccc gaacagggac ttgaaagcga aagggaaacc agaggagctc tctcgacgca
      241 ggactcggct tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc
      301 caaaaatttt gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta
      361 agcgggggag aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa
      421 aatataaatt aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc
      481 ctggcctgtt agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc
      541 ttcagacagg atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg
      601 tgcatcaaag gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc
      661 aaaacaaaag taagaccacc gcacagcaag cggccgctga tcttcagacc tggaggagga
      721 gatatgaggg acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca
      781 ttaggagtag cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg
      841 ggaataggag ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcg
      901 tcaatgacgc tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac
      961 aatttgctga gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc
     1021 aagcagctcc aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg
     1081 gggatttggg gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt
     1141 tggagtaata aatctctgga acagatttgg aatcacacga cctggatgga gtgggacaga
     1201 gaaattaaca attacacaag cttaatacac tccttaattg aagaatcgca aaaccagcaa
     1261 gaaaagaatg aacaagaatt attggaatta gataaatggg caagtttgtg gaattggttt
     1321 aacataacaa attggctgtg gtatataaaa ttattcataa tgatagtagg aggcttggta
     1381 ggtttaagaa tagtttttgc tgtactttct atagtgaata gagttaggca gggatattca
     1441 ccattatcgt ttcagaccca cctcccaacc ccgaggggac ccgacaggcc cgaaggaata
     1501 gaagaagaag gtggagagag agacagagac agatccattc gattagtgaa cggatcggca
     1561 ctgcgtgcgc caattctgca gacaaatggc agtattcatc cacaatttta aaagaaaagg
     1621 ggggattggg gggtacagtg caggggaaag aatagtagac ataatagcaa cagacataca
     1681 aactaaagaa ttacaaaaac aaattacaaa aattcaaaat tttcgggttt attacaggga
     1741 cagcagagat ccagtttggt taattaatgc aaagatggat aaagttttaa acagagagga
     1801 atctttgcag ctaatggacc ttctaggtct tgaaaggagt gggaattggc tccggtgccc
     1861 gtcagtgggc agagcgcaca tcgcccacag tccccgagaa gttgggggga ggggtcggca
     1921 attgaaccgg tgcctagaga aggtggcgcg gggtaaactg ggaaagtgat gtcgtgtact
     1981 ggctccgcct ttttcccgag ggtgggggag aaccgtatat aagtgcagta gtcgccgtga
     2041 acgttctttt tcgcaacggg tttgccgcca gaacacaggt aagtgccgtg tgtggttccc
     2101 gcgggcctgg cctctttacg ggttatggcc cttgcgtgcc ttgaattact tccacctggc
     2161 tgcagtacgt gattcttgat cccgagcttc gggttggaag tgggtgggag agttcgaggc
     2221 cttgcgctta aggagcccct tcgcctcgtg cttgagttga ggcctggcct gggcgctggg
     2281 gccgccgcgt gcgaatctgg tggcaccttc gcgcctgtct cgctgctttc gataagtctc
     2341 tagccattta aaatttttga tgacctgctg cgacgctttt tttctggcaa gatagtcttg
     2401 taaatgcggg ccaagatctg cacactggta tttcggtttt tggggccgcg ggcggcgacg
     2461 gggcccgtgc gtcccagcgc acatgttcgg cgaggcgggg cctgcgagcg cggccaccga
     2521 gaatcggacg ggggtagtct caagctggcc ggcctgctct ggtgcctggc ctcgcgccgc
     2581 cgtgtatcgc cccgccctgg gcggcaaggc tggcccggtc ggcaccagtt gcgtgagcgg
     2641 aaagatggcc gcttcccggc cctgctgcag ggagctcaaa atggaggacg cggcgctcgg
     2701 gagagcgggc gggtgagtca cccacacaaa ggaaaagggc ctttccgtcc tcagccgtcg
     2761 cttcatgtga ctccacggag taccgggcgc cgtccaggca cctcgattag ttctcgagct
     2821 tttggagtac gtcgtcttta ggttgggggg aggggtttta tgcgatggag tttccccaca
     2881 ctgagtgggt ggagactgaa gttaggccag cttggcactt gatgtaattc tccttggaat
     2941 ttgccctttt tgagtttgga tcttggttca ttctcaagcc tcagacagtg gttcaaagtt
     3001 tttttcttcc atttcaggtg tcgtgacgta cggccaccat gagccccaag aagaagagaa
     3061 aggtggaggc cagcatcgaa aaaaaaaagt ccttcgccaa gggcatgggc gtgaagtcca
     3121 cactcgtgtc cggctccaaa gtgtacatga caaccttcgc cgaaggcagc gacgccaggc
     3181 tggaaaagat cgtggagggc gacagcatca ggagcgtgaa tgagggcgag gccttcagcg
     3241 ctgaaatggc cgataaaaac gccggctata agatcggcaa cgccaaattc agccatccta
     3301 agggctacgc cgtggtggct aacaaccctc tgtatacagg acccgtccag caggatatgc
     3361 tcggcctgaa ggaaactctg gaaaagaggt acttcggcga gagcgctgat ggcaatgaca
     3421 atatttgtat ccaggtgatc cataacatcc tggacattga aaaaatcctc gccgaataca
     3481 ttaccaacgc cgcctacgcc gtcaacaata tctccggcct ggataaggac attattggat
     3541 tcggcaagtt ctccacagtg tatacctacg acgaattcaa agaccccgag caccataggg
     3601 ccgctttcaa caataacgat aagctcatca acgccatcaa ggcccagtat gacgagttcg
     3661 acaacttcct cgataacccc agactcggct atttcggcca ggcctttttc agcaaggagg
     3721 gcagaaatta catcatcaat tacggcaacg aatgctatga cattctggcc ctcctgagcg
     3781 gactggcgca ctgggtggtc gctaacaacg aagaagagtc caggatctcc aggacctggc
     3841 tctacaacct cgataagaac ctcgacaacg aatacatctc caccctcaac tacctctacg
     3901 acaggatcac caatgagctg accaactcct tctccaagaa ctccgccgcc aacgtgaact
     3961 atattgccga aactctggga atcaaccctg ccgaattcgc cgaacaatat ttcagattca
     4021 gcattatgaa agagcagaaa aacctcggat tcaatatcac caagctcagg gaagtgatgc
     4081 tggacaggaa ggatatgtcc gagatcagga aaaatcataa ggtgttcgac tccatcagga
     4141 ccaaggtcta caccatgatg gactttgtga tttataggta ttacatcgaa gaggatgcca
     4201 aggtggctgc cgccaataag tccctccccg ataatgagaa gtccctgagc gagaaggata
     4261 tctttgtgat taacctgagg ggctccttca acgacgacca gaaggatgcc ctctactacg
     4321 atgaagctaa tagaatttgg agaaagctcg aaaatatcat gcacaacatc aaggaattta
     4381 ggggaaacaa gacaagagag tataagaaga aggacgcccc tagactgccc agaatcctgc
     4441 ccgctggccg tgatgtttcc gccttcagca aactcatgta tgccctgacc atgttcctgg
     4501 atggcaagga gatcaacgac ctcctgacca ccctgattaa taaattcgat aacatccaga
     4561 gcttcctgaa ggtgatgcct ctcatcggag tcaacgctaa gttcgtggag gaatacgcct
     4621 ttttcaaaga ctccgccaag atcgccgatg agctgaggct gatcaagtcc ttcgctagaa
     4681 tgggagaacc tattgccgat gccaggaggg ccatgtatat cgacgccatc cgtattttag
     4741 gaaccaacct gtcctatgat gagctcaagg ccctcgccga caccttttcc ctggacgaga
     4801 acggaaacaa gctcaagaaa ggcaagcacg gcatgagaaa tttcattatt aataacgtga
     4861 tcagcaataa aaggttccac tacctgatca gatacggtga tcctgcccac ctccatgaga
     4921 tcgccaaaaa cgaggccgtg gtgaagttcg tgctcggcag gatcgctgac atccagaaaa
     4981 aacagggcca gaacggcaag aaccagatcg acaggtacta cgaaacttgt atcggaaagg
     5041 ataagggcaa gagcgtgagc gaaaaggtgg acgctctcac aaagatcatc accggaatga
     5101 actacgacca attcgacaag aaaaggagcg tcattgagga caccggcagg gaaaacgccg
     5161 agagggagaa gtttaaaaag atcatcagcc tgtacctcac cgtgatctac cacatcctca
     5221 agaatattgt caatatcaac gccaggtacg tcatcggatt ccattgcgtc gagcgtgatg
     5281 ctcaactgta caaggagaaa ggctacgaca tcaatctcaa gaaactggaa gagaagggat
     5341 tcagctccgt caccaagctc tgcgctggca ttgatgaaac tgcccccgat aagagaaagg
     5401 acgtggaaaa ggagatggct gaaagagcca aggagagcat tgacagcctc gagagcgcca
     5461 accccaagct gtatgccaat tacatcaaat acagcgacga gaagaaagcc gaggagttca
     5521 ccaggcagat taacagggag aaggccaaaa ccgccctgaa cgcctacctg aggaacacca
     5581 agtggaatgt gatcatcagg gaggacctcc tgagaattga caacaagaca tgtaccctgt
     5641 tcgcaaacaa ggccgtcgcc ctggaagtgg ccaggtatgt ccacgcctat atcaacgaca
     5701 ttgccgaggt caattcctac ttccaactgt accattacat catgcagaga attatcatga
     5761 atgagaggta cgagaaaagc agcggaaagg tgtccgagta cttcgacgct gtgaatgacg
     5821 agaagaagta caacgatagg ctcctgaaac tgctgtgtgt gcctttcggc tactgtatcc
     5881 ccaggtttaa gaacctgagc atcgaggccc tgttcgatag gaacgaggcc gccaagttcg
     5941 acaaggagaa aaagaaggtg tccggcaatt ccggatccgg acctaagaaa aagaggaagg
     6001 tggcggccgc ttacccatac gatgttccag attacgctgc tagcggcaag ttgtctgaat
     6061 cagagattag gtttggactg aaaacggagg tgatgggcca acatcttatc tatcacgatg
     6121 ttttgtccag cacacaaaag acagcccatg aattggcaaa taacaatgcc cccgagggta
     6181 ccctcgtggt tgcagataaa cagacagccg gtaggggcgg gatgagccga gtgtggcatt
     6241 ctcaagaagg aaacggagta tggatgagtt tgatattgcg cccggacatt ccgctccaaa
     6301 agacgcccca acttaccttg ctggcagctg tagcggtggt tcagggaata gaagaggccg
     6361 cgggcataca gactgatata aagtggccga acgacatact cattaacggg aaaaaaacag
     6421 tagggatact taccgaaatg caagctgagg aggatcgagt gcgctccgtg attattggta
     6481 ttggcattaa tgtaaatcag caacccaatg atttccccga tgagctcaag gacattgcga
     6541 cgagtctgtc ccaggcagct ggggaaaaga tcgacagggc aggtgtaatc cagcatatct
     6601 tgctttgttt cgaaaagcga taccgagact acatgaccca cggtttcacg cccatcaagc
     6661 tcttgtggga gtcatacgcc ctcggtattg gcacgaacat gagggcgaga actctcaacg
     6721 ggacatttta cggtaaggcg ttgggtattg acgacgaagg ggtacttttg ttggaaacta
     6781 atgaaggcat taagaagata tacagcgcgg acatcagcct tagggctagc ggcagtggag
     6841 agggcagagg aagtctgcta acatgcggtg acgtcgagga gaatcctggc ccagtgagca
     6901 agggcgagga gctgttcacc ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa
     6961 acggccacaa gttcagcgtg tccggcgagg gcgagggcga tgccacctac ggcaagctga
     7021 ccctgaagtt catctgcacc accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca
     7081 ccctgaccta cggcgtgcag tgcttcagcc gctaccccga ccacatgaag cagcacgact
     7141 tcttcaagtc cgccatgccc gaaggctacg tccaggagcg caccatcttc ttcaaggacg
     7201 acggcaacta caagacccgc gccgaggtga agttcgaggg cgacaccctg gtgaaccgca
     7261 tcgagctgaa gggcatcgac ttcaaggagg acggcaacat cctggggcac aagctggagt
     7321 acaactacaa cagccacaac gtctatatca tggccgacaa gcagaagaac ggcatcaagg
     7381 tgaacttcaa gatccgccac aacatcgagg acggcagcgt gcagctcgcc gaccactacc
     7441 agcagaacac ccccatcggc gacggccccg tgctgctgcc cgacaaccac tacctgagca
     7501 cccagtccgc cctgagcaaa gaccccaacg agaagcgcga tcacatggtc ctgctggagt
     7561 tcgtgaccgc cgccgggatc actctcggca tggacgagct gtacaagtaa gaattcgata
     7621 tcaagcttat cgataatcaa cctctggatt acaaaatttg tgaaagattg actggtattc
     7681 ttaactatgt tgctcctttt acgctatgtg gatacgctgc tttaatgcct ttgtatcatg
     7741 ctattgcttc ccgtatggct ttcattttct cctccttgta taaatcctgg ttgctgtctc
     7801 tttatgagga gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg
     7861 acgcaacccc cactggttgg ggcattgcca ccacctgtca gctcctttcc gggactttcg
     7921 ctttccccct ccctattgcc acggcggaac tcatcgccgc ctgccttgcc cgctgctgga
     7981 caggggctcg gctgttgggc actgacaatt ccgtggtgtt gtcggggaaa tcatcgtcct
     8041 ttccttggct gctcgcctgt gttgccacct ggattctgcg cgggacgtcc ttctgctacg
     8101 tcccttcggc cctcaatcca gcggaccttc cttcccgcgg cctgctgccg gctctgcggc
     8161 ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat ctccctttgg gccgcctccc
     8221 cgcatcgata ccgtcgacct cgagacctag aaaaacatgg agcaatcaca agtagcaata
     8281 cagcagctac caatgctgat tgtgcctggc tagaagcaca agaggaggag gaggtgggtt
     8341 ttccagtcac acctcaggta cctttaagac caatgactta caaggcagct gtagatctta
     8401 gccacttttt aaaagaaaag gggggactgg aagggctaat tcactcccaa cgaagacaag
     8461 atatccttga tctgtggatc taccacacac aaggctactt ccctgattgg cagaactaca
     8521 caccagggcc agggatcaga tatccactga cctttggatg gtgctacaag ctagtaccag
     8581 ttgagcaaga gaaggtagaa gaagccaatg aaggagagaa cacccgcttg ttacaccctg
     8641 tgagcctgca tgggatggat gacccggaga gagaagtatt agagtggagg tttgacagcc
     8701 gcctagcatt tcatcacatg gcccgagagc tgcatccgga ctgtactggg tctctctggt
     8761 tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc
     8821 aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta
     8881 actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagg gcccgtttaa
     8941 acccgctgat cagcctcgac tgtgccttct agttgccagc catctgttgt ttgcccctcc
     9001 cccgtgcctt ccttgaccct ggaaggtgcc actcccactg tcctttccta ataaaatgag
     9061 gaaattgcat cgcattgtct gagtaggtgt cattctattc tggggggtgg ggtggggcag
     9121 gacagcaagg gggaggattg ggaagacaat agcaggcatg ctggggatgc ggtgggctct
     9181 atggcttctg aggcggaaag aaccagctgg ggctctaggg ggtatcccca cgcgccctgt
     9241 agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc
     9301 agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc
     9361 tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg
     9421 cacctcgacc ccaaaaaact tgattagggt gatggttcac gtagtgggcc atcgccctga
     9481 tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc
     9541 caaactggaa caacactcaa ccctatctcg gtctattctt ttgatttata agggattttg
     9601 ccgatttcgg cctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaattaa
     9661 ttctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca gcaggcagaa
     9721 gtatgcaaag catgcatctc aattagtcag caaccaggtg tggaaagtcc ccaggctccc
     9781 cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccata gtcccgcccc
     9841 taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct
     9901 gactaatttt ttttatttat gcagaggccg aggccgcctc tgcctctgag ctattccaga
     9961 agtagtgagg aggctttttt ggaggcctag gcttttgcaa aaagctcccg ggagcttgta
    10021 tatccatttt cggatctgat cagcacgtgt tgacaattaa tcatcggcat agtatatcgg
    10081 catagtataa tacgacaagg tgaggaacta aaccatggcc aagttgacca gtgccgttcc
    10141 ggtgctcacc gcgcgcgacg tcgccggagc ggtcgagttc tggaccgacc ggctcgggtt
    10201 ctcccgggac ttcgtggagg acgacttcgc cggtgtggtc cgggacgacg tgaccctgtt
    10261 catcagcgcg gtccaggacc aggtggtgcc ggacaacacc ctggcctggg tgtgggtgcg
    10321 cggcctggac gagctgtacg ccgagtggtc ggaggtcgtg tccacgaact tccgggacgc
    10381 ctccgggccg gccatgaccg agatcggcga gcagccgtgg gggcgggagt tcgccctgcg
    10441 cgacccggcc ggcaactgcg tgcacttcgt ggccgaggag caggactgac acgtgctacg
    10501 agatttcgat tccaccgccg ccttctatga aaggttgggc ttcggaatcg ttttccggga
    10561 cgccggctgg atgatcctcc agcgcgggga tctcatgctg gagttcttcg cccaccccaa
    10621 cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa
    10681 taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta
    10741 tcatgtctgt ataccgtcga cctctagcta gagcttggcg taatcatggt catagctgtt
    10801 tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa
    10861 gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact
    10921 gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc
    10981 ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg
    11041 ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc
    11101 cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag
    11161 gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca
    11221 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca
    11281 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg
    11341 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag
    11401 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt
    11461 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca
    11521 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg
    11581 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt
    11641 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc
    11701 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg
    11761 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg
    11821 gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta
    11881 gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg
    11941 gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg
    12001 ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc
    12061 atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc
    12121 agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc
    12181 ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag
    12241 tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat
    12301 ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg
    12361 caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt
    12421 gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag
    12481 atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg
    12541 accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt
    12601 aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct
    12661 gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac
    12721 tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat
    12781 aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat
    12841 ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca
    12901 aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtcgacg gatcgggaga
    12961 tctcccgatc ccctatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc
    13021 cagtatctgc tccctgcttg tgtgttggag gtcgctgagt agtgcgcgag caaaatttaa
    13081 gctacaacaa ggcaaggctt gaccgacaat tgcatgaaga atctgcttag ggttaggcgt
    13141 tttgcgctgc ttcgcgatgt acgggccaga tatacgcgtt gacattgatt attgactagt
    13201 tattaatagt aatcaattac ggggtcatta gttcatagcc catatatgga gttccgcgtt
    13261 acataactta cggtaaatgg cccgcctggc tgaccgccca acgacccccg cccattgacg
    13321 tcaataatga cgtatgttcc catagtaacg ccaataggga ctttccattg acgtcaatgg
    13381 gtggagtatt tacggtaaac tgcccacttg gcagtacatc aagtgtatca tatgccaagt
    13441 acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc ccagtacatg
    13501 accttatggg actttcctac ttggcagtac atctacgtat tagtcatcgc tattaccatg
    13561 gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc acggggattt
    13621 ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac
    13681 tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag gcgtgtacgg
    13741 tgggaggtct atataagcag cgcgttttgc ctgtact
//
