LOCUS       sequence-30451-e         684 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 684)
  AUTHORS   Wilson AA, Kwok LW, Hovav AH, Ohle SJ, Little FF, Fine A, Kotton DN
  TITLE     Sustained expression of alpha1-antitrypsin after transplantation of 
            manipulated hematopoietic stem cells.
  JOURNAL   Am J Respir Cell Mol Biol. 2008 Aug . 39(2):133-41.
  PUBMED    18323534
REFERENCE   2  (bases 1 to 684)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Am J Respir
            Cell Mol Biol. 2008 Aug . 39(2):133-41."
COMMENT     Sequence Label: pHAGE-CMV-dsRed-UBC-GFP-W
FEATURES             Location/Qualifiers
     source          1..684
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    30..618
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(83..103)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(501..512)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 gagagctgta cagtaaatcg atagatccta atcaacctct ggattacaaa atttgtgaaa
       61 gattgactgg tattcttaac tatgttgctc cttttacgct atgtggatac gctgctttaa
      121 tgcctttgta tcatgctatt gcttcccgta tggctttcat tttctcctcc ttgtataaat
      181 cctggttgct gtctctttat gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt
      241 gcactgtgtt tgctgacgca acccccactg gttggggcat tgccaccacc tgtcagctcc
      301 tttccgggac tttcgctttc cccctcccta ttgccacggc ggaactcatc gccgcctgcc
      361 ttgcccgctg ctggacaggg gctcggctgt tgggcactga caattccgtg gtgttgtcgg
      421 ggaaatcatc gtcctttcct tggctgctcg cctgtgttgc cacctggatt ctgcgcggga
      481 cgtccttctg ctacgtccct tcggccctca atccagcgga ccttccttcc cgcggcctgc
      541 tgccggctct gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt cggatctccc
      601 tttgggccgc ctccccgcct gagatccttt aagaccaatg acttacaagg cagctgtaga
      661 tcttagccac tttttaaaag aaaa
//
