LOCUS       sequence-30453-e         696 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral overexpression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 696)
  AUTHORS   Wilson AA, Murphy GJ, Hamakawa H, Kwok LW, Srinivasan S, Hovav AH, 
            Mulligan RC, Amar S, Suki B, Kotton DN
  TITLE     Amelioration of emphysema in mice through lentiviral transduction of
            long-lived pulmonary alveolar macrophages.
  JOURNAL   J Clin Invest. 2010 Jan . 120(1):379-89.
  PUBMED    20038801
REFERENCE   2  (bases 1 to 696)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Clin 
            Invest. 2010 Jan . 120(1):379-89."
COMMENT     Sequence Label: pHAGE-EF1alphaL-hAAT-W
FEATURES             Location/Qualifiers
     source          1..696
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(194..214)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(612..623)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 cgtttttaga ggcataccca tgtctatccc ccccgaggtc aagttcaaca aaccctttgt
       61 cttcttaatg attgaacaaa ataccaagtc tcccctcttc atgggaaaag tggtgaatcc
      121 cacccaaaaa taaagatcct aatcaacctc tggattacaa aatttgtgaa agattgactg
      181 gtattcttaa ctatgttgct ccttttacgc tatgtggata cgctgcttta atgcctttgt
      241 atcatgctat tgcttcccgt atggctttca ttttctcctc cttgtataaa tcctggttgc
      301 tgtctcttta tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt
      361 ttgctgacgc aacccccact ggttggggca ttgccaccac ctgtcagctc ctttccggga
      421 ctttcgcttt ccccctccct attgccacgg cggaactcat cgccgcctgc cttgcccgct
      481 gctggacagg ggctcggctg ttgggcactg acaattccgt ggtgttgtcg gggaaatcat
      541 cgtcctttcc ttggctgctc gcctgtgttg ccacctggat tctgngcggg acgtccttct
      601 gctacgtccc ttcggccctc aatccagcgg acctnccttc ccgcggcctg ctgccggctc
      661 tgcggcctct tccgcgtctt cgccttcgcc ctcaga
//
