LOCUS       sequence-304544-       14788 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Non-infectious HIV-1 Clone with stop codon in Env (see NIH AIDS 
            Reagent Program Cat #11051) and M66I mutation in capsid (CA M66I)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14788)
  AUTHORS   Christensen DE, Ganser-Pornillos BK, Johnson JS, Pornillos O, 
            Sundquist WI
  TITLE     Reconstitution and visualization of HIV-1 capsid-dependent 
            replication and integration in vitro.
  JOURNAL   Science. 2020 Oct 9;370(6513). pii: 370/6513/eabc8420. doi: 
            10.1126/science.abc8420.
  PUBMED    33033190
REFERENCE   2  (bases 1 to 14788)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science."; 
            date: "2020-10-9"; volume: "370(6513). pii: 370/6513/eabc8420. doi";
            pages: " 10.1126/science.abc8420"
COMMENT     Sequence Label: SG3-Delta-ENV CA M66I
FEATURES             Location/Qualifiers
     source          1..14788
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    47..172
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     CDS             1451..4462
                     /codon_start=1
                     /gene="pol"
                     /product="pol protein from human immunodeficiency virus 1"
                     /label=HIV-1 pol
                     /translation="FFREDLAFPQGKAREFSSEQTRANSSTRRELQVWGGDNNSPSEAG
                     AGRQGNVSLSFPQITLWQRPLVTIKIGGQLKEALLDTGADDTVLEEMNLPGRWKPKMIG
                     GIGGFIKVRQYDQIPIEICGHKAIGTVLVGPTPVNIIGRNLLTQIGCTLNFPISPIETV
                     PVKLKPGMDGPKVKQWPLTEEKIKALVEICTEMEKEGKISKIGPENPYNTPVFAIKKKD
                     STKWRKLVDFRELNKRTQDFWEVQLGIPHPAGLKKKKSVTVLDVGDAYFSVPLDKDFRK
                     YTAFTIPSINNETPGIRYQYNVLPQGWKGSPAIFQSSMTKILEPFRKQNPDIVIYQYMD
                     DLYVGSDLEIGQHRTKIEELRQHLLRWGFTTPDKKHQKEPPFLWMGYELHPDKWTVQPI
                     VLPEKDSWTVNDIQKLVGKLNWASQIYSGIKVKQLCKLLRGTKALTEVVTLTEEAELEL
                     AENREILKEPVHGVYYDPSKDLIAEIQKQGQGQWTYQIYQEPFKNLKTGKYARTRGAHT
                     NDVKQLTEAVQKITTECIIIWGKTPKFRLPIQKETWETWWTEYWQATWIPEWEFVNTPP
                     LVKLWYQLEKEPIEGAETFYVDGAANRETKLGKAGYVTNKGRQKVVTLTDTTNQKTELE
                     AIHLALQDSGLEVNIVTDSQYALGIIQAQPDKSESELVSQIIEQLIKKEKVYLAWVPAH
                     KGIGGNEQVDKLVSAGVRKVLFLDGIDKAQEEHEKYHSNWRAMASDFNLPPVVAKEIVA
                     SCDKCQLKGEAMHGQVDCSPGIWQLDCTHLEGKIILVAVHVASGYIEAEVIPAETGQET
                     AYFLLKLAGRWPVKTIHTDNGSNFTSTTVKAACWWAGIKQEFGIPYNPQSQGVVESMNK
                     ELKKIIGQVRDQAEHLKTAVQMAVFIHNFKRKGGIGGYSAGERIVDIIATDIQTKELQK
                     QITKIQNFRVYYRDNKDPLWKGPAKLLWKGEGAVVIQDNSDIKVVPRRKAKIIRDYGKQ
                     MAGDDCVASRQDED"
     misc_feature    4147..4264
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     RBS             6054..6065
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     misc_feature    7147..7380
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             7565..7609
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     LTR             8841..9097
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     RBS             9298..9306
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     primer_bind     complement(9586..9605)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(9587..9605)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(9624..9640)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9624..9640)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9637..9659)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9648..9664
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9672..9678,9679..9696,9697..9702))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9717..9738
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9855..9872)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(10026..10614)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10106..10125)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10785..11576,11577..11645))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11408..11427
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11646..11750)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      11777..12232
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(11864..11883)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     12074..12095
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     12358..12380
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     12372..12389
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     12373..12389
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     LTR             14532..14788
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
ORIGIN
        1 gtggcgcccg aacagggacc ggaaagcgaa agagaaacca gaggagctct ctcgacgcag
       61 gactcggctt gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc
      121 aaaaattttg actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattga
      181 gcgggggaaa attggataag tgggagaaaa ttcggttaag gccaggggga aagaaaaaat
      241 ataaattaaa acatctagta tgggcaagca gggagctaga acgattcgca gttaatcccg
      301 gcctgttaga aacagcagaa ggctgtagac aaatactggg acagctacaa ccgtcccttc
      361 agacaggatc agaagaactt aaatcattat ataatacaat agcagtcctc tattgtgtgc
      421 atcaaatgat agatgtaaaa gacaccaagg aagctttaga gaagatagag gaagagcaaa
      481 acaacagtaa gaaaaaagca cagcaagcag cagctgacac aggaaacagc agccaggtca
      541 gccgaaatta ccctatagtg cagaacatcc aggggcaaat ggtacatcag gccatatcac
      601 ccagaacttt aaatgcatgg gtaaaagtag tagaagagaa ggctttcagc ccagaagtaa
      661 tacccatgtt ttcagcatta tcagaaggag ccaccccaca agatttaaac accatgctaa
      721 acacagtggg gggacatcaa gcagctatcc aaatgttaaa agagaccatc aatgaggaag
      781 ctgcagaatg ggatagattg catccagtgc aagcagggcc tgttgcacca ggccagatga
      841 gagaaccaag gggaagtgac atagcaggaa ctactagtac ccttcaggaa caaataggat
      901 ggatgacaaa taatccacct atcccagtag gagaaatcta taaaagatgg ataatcctgg
      961 gattaaataa aatagtaagg atgtatagtc cttctagcat tctggacata agacaaggac
     1021 caaaggaatc ctttagagac tatgtagaca ggttctataa aactttaaga gccgagcaag
     1081 cttcacagga ggtaaaaaat tggatgacag aaaccttgtt ggtccaaaat gcgaacccag
     1141 attgtaagac cattttaaaa gcattgggac caggagctac actagaggaa atgatgacag
     1201 catgtcaggg agtgggagga cccggccaca aagcaagagt tttggcggaa gcaatgagcc
     1261 aagtaacaaa ttcagccacc ataatgatgc agagaggcaa ttttaggaac caaagaaaga
     1321 ctgttaagtg tttcaattgt ggcaaagaag ggcatatagc cagaaattgc aaggccccta
     1381 ggaaaagggg ctgctggaaa tgtggaaagg aaggacacca aatgaaagat tgtacagaga
     1441 gacaggctaa ttttttaggg aagatctggc cttcccacaa gggaaggcca gggaattttc
     1501 ctcagagcag actagagcca acagctccac cagaagagag cttcaggttt ggggaggaga
     1561 caacaactcc ccctcagaag caggagcggg aagacaagga aatgtatccc ttagcttccc
     1621 tcagatcact ctttggcaac gacccctcgt cacaataaag ataggggggc aactaaagga
     1681 ggctctatta gatacaggag cagatgatac agtattagaa gaaatgaatt tgccagggag
     1741 atggaagcca aaaatgatag ggggaattgg aggttttatc aaagtaagac agtatgatca
     1801 gatacccata gaaatctgtg gacataaagc tataggtaca gtattagtag gacctacacc
     1861 tgtcaacata attggaagaa atctgttgac tcagattggt tgcactctaa attttcccat
     1921 tagtcctatt gaaactgtac cagtaaaatt aaagccagga atggatggcc caaaagttaa
     1981 acaatggcca ttgacagaag aaaaaataaa agcattagta gaaatttgta cagaaatgga
     2041 aaaggaaggg aagatttcaa aaattgggcc tgaaaatcca tacaatactc cagtatttgc
     2101 tataaagaaa aaagacagta ctaaatggag aaaattagta gatttcagag aacttaataa
     2161 gaggactcaa gacttctggg aagttcaatt aggaatacca catcccgctg gattaaaaaa
     2221 gaaaaaatca gtaacagtac tagatgtggg tgatgcatat ttctcagttc ccttagataa
     2281 agacttcagg aaatatactg catttaccat acctagtata aacaatgaga caccagggat
     2341 tagatatcag tacaatgtgc tcccacaggg atggaaagga tcaccagcaa tattccaaag
     2401 tagcatgaca aaaatcttag agccttttag aaagcaaaat ccagacatag ttatctatca
     2461 gtacatggat gatttgtatg taggatctga cttagaaata gggcagcata gaacaaaaat
     2521 agaggaactg agacaacatc tgttaaggtg gggatttacc acaccagaca aaaaacatca
     2581 gaaagaacct ccattccttt ggatgggtta tgaactccat cctgataaat ggacagtaca
     2641 gcctatagtg ctgccagaaa aagacagctg gactgtcaat gacatacaga agttagtggg
     2701 aaaattgaat tgggcaagtc agatttactc agggatcaaa gtgaagcagt tatgtaaact
     2761 ccttagggga accaaagcac taacagaagt agtaacacta acagaagaag cagagctaga
     2821 actggcagaa aacagggaaa ttctaaaaga accagtacat ggagtgtatt atgacccatc
     2881 aaaagactta atagcagaaa tacagaaaca ggggcaaggc caatggacat atcaaattta
     2941 tcaagagcca tttaaaaatc tgaaaacagg aaaatatgca agaacgaggg gtgcccacac
     3001 taatgatgta aaacaattaa cagaggcagt gcaaaaaata accacagaat gcataataat
     3061 atggggaaaa actcctaaat ttagactgcc catacaaaaa gaaacatggg aaacatggtg
     3121 gacagagtat tggcaagcca cctggattcc tgaatgggag tttgtcaata cccctccctt
     3181 agtgaaatta tggtaccagt tagagaaaga gcccatagaa ggcgcagaaa ctttctatgt
     3241 agatggagca gctaacaggg agactaaatt aggaaaagca ggatatgtta ctaacaaagg
     3301 aagacaaaaa gttgtcaccc taactgacac aacaaatcag aagactgagt tagaagcaat
     3361 tcatctagct ttgcaggatt ctggattaga agtaaacata gtaacagact cacaatatgc
     3421 attaggaatc attcaagcac aaccagataa aagtgaatca gaattagtca gtcaaataat
     3481 agagcagtta ataaaaaagg aaaaggtcta cctggcatgg gtaccagcac acaaaggaat
     3541 tggaggaaat gaacaagtag ataaattagt cagtgctggg gtcaggaaag tactattttt
     3601 agatggaata gataaggccc aagaagaaca tgagaaatat cacagtaatt ggagagccat
     3661 ggctagtgat tttaacttac cacctgtagt agcaaaagaa atagtagcca gctgtgataa
     3721 atgtcagcta aaaggagaag ccatgcatgg acaagtagac tgtagtccag gaatatggca
     3781 actagattgc acacatctag aaggaaaaat tatcctggtg gcggttcatg tagccagtgg
     3841 atatatagaa gcagaagtta ttccagcaga gacagggcag gaaacagcat actttctctt
     3901 aaaattagca ggaagatggc cagtaaaaac aatacataca gacaatggca gcaatttcac
     3961 cagtaccacg gttaaggccg cctgttggtg ggcagggatc aagcaggaat ttggcattcc
     4021 ctacaatccc caaagtcaag gagtagtaga atctatgaat aaagaattaa agaaaattat
     4081 aggacaggta agagatcagg ctgaacatct taaaacagca gtacaaatgg cagtatttat
     4141 ccacaatttt aaaagaaaag gggggattgg ggggtacagt gcaggggaaa gaatagtaga
     4201 cataatagca acagacatac aaactaaaga actacaaaaa caaattacaa aaattcaaaa
     4261 ttttcgggtt tattacaggg acaacaaaga tccactttgg aaaggaccag caaagcttct
     4321 ctggaaaggt gaaggggcag tagtaataca agataatagt gacataaaag tagtgccaag
     4381 aagaaaagca aagatcatta gagattatgg aaaacagatg gcaggtgatg attgtgtggc
     4441 aagtagacag gatgaggatt agaacatgga taagtttagt aaaacaccat atgtatattt
     4501 caaggaaagc aaagggatgg ttttatagac atcactatga aagcactcac ccaaaaataa
     4561 gttcagaagt acacatccca ctaggggatg ctagattggt aataacaaca tattggggtc
     4621 tgcatacagg agaaagagat tggcatttgg gtcatggagt ctccgtagaa tggaggaaaa
     4681 agagatatag cacacaagta gaccctgacc tagcagacca actaattcat ctgtattact
     4741 ttgattgttt ttcagaatct gccataagaa atgccatatt aggacatata gttagtccta
     4801 ggtgtgaata tcaagcagga cataacaagg taggatctct acagtaccta gcactagcag
     4861 cattaataac accaaaaagg ataaagccac ctttgcctag tgttacaaaa ctaacagagg
     4921 atagatggaa caagccccag aagaccaagg gccacagagg gagccataca atgaatggac
     4981 actagagctt ttagaagaac ttaagaatga agctgttaga cattttccta ggatatggct
     5041 ccatggctta gggcaatata tctatgaaac ttatggggat acttgggcag gagtggaagc
     5101 cctagtaaga actctgcaac aactgctgtt tactcttttc agaattgggt gtcgacatag
     5161 cagaataggc attactcaac gaagaagagc aagaaatgga gccagtagat cctagactag
     5221 agccctggaa gcatccagga agccagccta aaactgcttg taccaaatgc tattgtaaaa
     5281 agtgttgctt acattgccaa gtttgtttca tgacaaaagg cttaggcatc tcctatggca
     5341 ggaagaagcg gagacagcga cgaagagctc ctcaagacag tcaggctcat caagcttctc
     5401 tatcaaagca gtaagtagtg catgtaatgc aacctataca aatagcagca atagtagcat
     5461 tagtagtggt aggaataata gcaatagttg tgtggtccat agtattaata gaatatagga
     5521 aaatattaag acaaagaaaa atagacaggt taattaaaag aataagtaaa agagcagaag
     5581 acagtggcaa tgagagtgaa ggagatcagg aagaattatc agcacttgtg gagatggggc
     5641 accatgctct ttgggatatt gatgatctat agtgctgcag ggaatttgtg ggtcacagta
     5701 tattatgggg tacctgtatg gaaagaagca accaccactc tattttgtgc atcagatgct
     5761 aaagcatatg atacagaggt acataatgtt tgggccacac atgcctgtat acccacagac
     5821 cccaacccac aagagatagt attagaaaat gtgacagaag attttaacat gtggaaaaat
     5881 aacatggtag aacagatgca ggaggatata atcagtttat gggatcaaag cctaaagcca
     5941 tgtgtaaaat taaccccact ctgtgttact ctaaattgca ctgatgagtt gaagaatgct
     6001 actaatacca ctagctagta ctaatacccc tagtggtagc tggaaaaaga tggaaagagg
     6061 agaaataaag aactgctctt tcaatgtctt aggagataag aagcagaaag catatgcact
     6121 cttttataaa cttgatgtag taccaataga taatgataag aatagtacca aatataggtt
     6181 gataagttgt aacacctcag tcactacaca ggcctgtcca aagatatcct ttgagccaat
     6241 tcccatacat tattgtgccc cggctggttt tgcgattcta aagtgtaaag ataagaagtt
     6301 caatggaaca ggatcatgta aaaatgtcag cacagtacaa tgtacacatg gaattaagcc
     6361 agtagtatca actcaactgc tgttaaatgg cagcctagca gaagaagagg tagtaattag
     6421 atctgccaat ttctcggaca atgctaaaac cataatagta cagctgaaag aagctgtaga
     6481 aattaattgc acaagaccca acaaaaaaac aagaaaacgt ataactacgg gaccagggag
     6541 agtatattat acaacaggag aaatagtagg agatataaga caagcacatt gtaaccttag
     6601 tagagcaaaa tggaatgaca ctttaagaca gatagttata aaattaagag aacaatttga
     6661 gaataaaaca atagtcttta atcaatcctc aggaggggac ccagaaattg taatgcatag
     6721 ttttaattgt ggaggggaat ttttctactg taagtcaaca caactgttta atagtacttg
     6781 ggctgggaat aatacttgga atagtagtgc tgaaaggtca gatgacactg gaggaaatat
     6841 cacactccca tgcagaataa aacaaattat aaacatgtgg caggaagtag gaaaagcaat
     6901 gtatgcccct cccatcagcg gacaaattag atgtacatca aatattacag ggctgctatt
     6961 aacaagagat ggtggtacga gcgataccaa taccaccgag accttcagac ctggaggagg
     7021 agatatgagg gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc
     7081 attaggagta gcacccacca cggcaaagag aagagtggtg caaagagaaa aaagagcagt
     7141 gggaatagga gctctgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc
     7201 gtcaatgacg ttgacggtac aggccagaca attattgtct ggtatagtgc aacagcagaa
     7261 caatttgctg agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat
     7321 caagcagctc caggcaagag tcctggctgt ggaaagatac ctaaaggatc aacagctcct
     7381 ggggatttgg ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag
     7441 ttggagtaat aaatctctga atcagatttg ggataacatg acttggatgc agtgggaaag
     7501 agaaattgaa aattacacag acttaatata caacttaatt gaagaatcgc agaaccagca
     7561 agaaaagaat gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt
     7621 tacaataaca aactggctgt ggtatataaa aatattcata atgatagtag gaggcttgat
     7681 aggtttaaga atagttttta ctgtactttc tatagtgaat agagttaggc agggatactc
     7741 accattgtcg tttcagaccc acctcccaac cccgagggga cccgacaggc ccgaaggaat
     7801 cgaagaagaa ggtggagaga gagacagaga cagatccggt cgattagtga acggattctt
     7861 agcacttttc tgggacgatc tgcggagcct gtgcctcttc agctaccacc gcttgagaga
     7921 cttaatcttg gttgtaacga ggattgtgga acttctggga cgcagggggt gggaagccct
     7981 caagtattgg tggagtctcc tacagtattg gagccaggaa ctaaagaata gtgctgttaa
     8041 cttgcttaat gtcacagcca tagcagtagc tgagggaaca gatagggtta tagaagtagt
     8101 acaaagaact tatagagcta ttctccacat acctagaaga ataagacagg gcttggaaag
     8161 gcttttgcta taagatgggt ggcaagtggt caaaacgtat ggagggtgga tggcatgctg
     8221 taagggaaag aatgagacga gctgagccag cagcagatgg ggtgggagca gtatctagag
     8281 acctggaaaa acatggagca atcacaagta gcaatacagc agctaacaat gctgattgtg
     8341 cctggctgga agcacaagag gaggaggagg tgggttttcc agtcagacct caggtacctt
     8401 taagaccaat aacttacaag gcagctgtag atattagcca ctttttaaaa gaaaaggggg
     8461 gactggaagg gctaattttc tcccaaagaa gacaagatat ccttgatctg tggacctacc
     8521 acacacaagg ctacttccct gattggcaga actacacacc agggccaggg atcagatatc
     8581 cactgacctt tggatggtgt ttcaagctag taccagttga tccagagaag atagaagagg
     8641 ccaatgaagg agaaaacaac agcttgctac accctatgag ccagcatggg atggatgacc
     8701 cggagagaga agtgctagag tggaggtttg acagccgcct agcatttcat cacatggccc
     8761 gagagctgca tccggagtac tacaagaacc gatgacatcg cgctttctac aagggacttt
     8821 ccgctgggga ctttccaggg gaggcgtggc ctgggcggga ccggggagtg gcgagccctc
     8881 agatgctgca tataagcagc tgcttttgcc tgtactgggt ctctctggtt agaccagatc
     8941 tgagcctggg agctctctgg ctagctaggg aacccactgc ttaagcctca ataaagcttg
     9001 ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtag ctagagatcc
     9061 ctcataccct tttagtcagt gtggaaaatc tctagcaaaa attcctagct gcctagaaat
     9121 aaaacactat gcttttgaat aacatatgac ttaaaagaag aatttatgat ggctattaga
     9181 aaatatttta aactgattga aaatgaaggt gaactacaat gatctagatt gctgctgctg
     9241 aaatgctaag atgaaattta tagccttaag tctatgcatt ggaaaagaaa gcttttttaa
     9301 ggaggtagaa aaggaatagg aaatcaaacc taaagacagt agaagtaaag aaataatgaa
     9361 gacaagagca gaaattagtg aatagaaagt aatcttacat agagaaaatt gacaaagcta
     9421 agaaaaagtt ggttctttga aaagattaat gtaattgacc acttagcaaa actgataaaa
     9481 ggaggatggg gagggggaga gagaacataa atttaaataa taggaattga aaaatgtcac
     9541 atcactacgg atcctctaga gtcgacctgc aggcatgcaa gctttcccta tagtgagtcg
     9601 tattagagct tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc
     9661 acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga
     9721 gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg
     9781 tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg
     9841 cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg
     9901 gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga
     9961 aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg
    10021 gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag
    10081 aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc
    10141 gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg
    10201 ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt
    10261 cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc
    10321 ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc
    10381 actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg
    10441 tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca
    10501 gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc
    10561 ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat
    10621 cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt
    10681 ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt
    10741 tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc
    10801 agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc
    10861 gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata
    10921 ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg
    10981 gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc
    11041 cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct
    11101 acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa
    11161 cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt
    11221 cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca
    11281 ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac
    11341 tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca
    11401 atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt
    11461 tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc
    11521 actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca
    11581 aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata
    11641 ctcatactct tcctttttca atattattga agcatttatc agggttattg tctcatgagc
    11701 ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc
    11761 cgaaaagtgc cacctgacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt
    11821 acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc
    11881 ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct
    11941 ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat
    12001 ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc
    12061 acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc tatctcggtc
    12121 tattcttttg atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg
    12181 atttaacaaa aatttaacgc gaattttaac aaaatattaa cgcttacaat ttccattcgc
    12241 cattcaggct gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc
    12301 agctggcgaa agggggatgt gctgcaaggc gattaagttg ggtaacgcca gggttttccc
    12361 agtcacgacg ttgtaaaacg acggccagtg aattccttct atggaatact ttttaaatat
    12421 atagtctgat aatatataac tgacacgttt tctgttgttt cataatggtt gattttctca
    12481 gggctacgtt tctctatttt ttcctgttct caaatcttaa ctctccatct ctgtcctcaa
    12541 gtggtaagtg atcttgtgtc ttattttgaa attaaagtca tcaacaaatt atttcaactc
    12601 atagatttat agttgtggaa gacttactat tcaaatggaa ttagaacgtt ttgggggata
    12661 ggatgcagta gttcttggta gtccaagact cttaataaaa caactagaaa agccaggtaa
    12721 tttacaaaat ttatatattt agcgatagag aacaatgggt gtaaaaatga ttaaagaaag
    12781 caaaattttg caccctgggg gaataccttt cacaagtgag ctgagaggtc tgcagctgat
    12841 cattgttttt cctgggtaca gagtagagga ggcctttggg ctgcagcctg agtggcagat
    12901 tggaaatatg aggggccttt aaacacagag aacgccctca gtatattcct aaattctgta
    12961 gttcagctgg gcggaagaga taaaccaaaa tctcagaaaa ggagattggg atattttcta
    13021 gtcctatggt gttgagataa agattctcta ggggaagcga cttgagaaca ataggtgaga
    13081 gttgaaagcg tttgaggaag tggtgaatac acactgatga ttttcaacac acccagtttc
    13141 ttcccccgta gacatttgcc aaattctgaa gctgcttagg ctagagagct aaaccagaag
    13201 tctcttaaaa acaaagcaat tcttatagta tctaggtacc tagaagataa cgatctgcta
    13261 gcttttgaaa tgaaggctct gcagagtctc atcctaggaa cagggacaaa ccagaggtag
    13321 tctccttcat agcaaaactg ctactcagca gtgttctatg attctggcat tatagaggaa
    13381 gtagtaaaag tatatgtatg aaaattgtgt taagtgaagg atgaataaga aaatatctag
    13441 ggtaaccact aaaagaaaag aatgtgtaac aaatgaatac agaaaaaaat tggatataga
    13501 atatgtgata aattcagaag aagacaagaa agaaaatata aaacaagcag gcaactaaat
    13561 tagtaattat gttacatgga aatgggctaa atatttcaag taaatggcaa aaatcatcag
    13621 actggacaaa taataattac ctaaatataa gtacctagaa agattgagag aaaatgatgc
    13681 gaaaaggtat accatccaaa tgctaactga aggaaataag tgtggctata ccaatcaggc
    13741 aaaggagact ttaatacaat aaacagtagt agaaaaagag aactacattt tatagtgtaa
    13801 ggggattgat ccaccagagt taccacaatc ctttatgtgt acccagtaaa atagcccaaa
    13861 catctataaa ccaaaaattt tattttgaac taaaagaaac agacaaatat gccctcacaa
    13921 tggatgcaca tatataggac atacatacat cacaattagc aggcttgatc taattgtcat
    13981 acgtagaaca ttgaaactat taatggtggt attaaccatc ttttcccttg cacatggaaa
    14041 ttgtactaga attgaccata agctagggcc atgaacaaag cctcagcaaa tatcattgat
    14101 tttaataatt gaatatgttg tttgtattat agtaaataaa aataactaga aaattggaag
    14161 ggctaattat ctcccaaaga agacaagata tccttgatct gtggacctac cacacacaag
    14221 gctacttccc tgattggcag aactacacac cagggccagg gatcagatat ccactgacct
    14281 ttggatggtg tttcaagcta gtaccagttg atccagagaa gatagaagag gccaatgaag
    14341 gagaaaacaa cagcttgcta caccctatga gccagcatgg gatggatgac ccggagagag
    14401 aagtgctaga gtggaggttt gacagccgcc tagcatttca tcacatggcc cgagagctgc
    14461 atccggagta ctacaagaac cgatgacatc gcgctttcta caagggactt tccgctgggg
    14521 actttccagg ggaggcgtgg cctgggcggg accggggagt ggcgagccct cagatgctgc
    14581 atataagcag ctgcttttgc ctgtactggg tctctctggt tagaccagat ctgagcctgg
    14641 gagctctctg gctagctagg gaacccactg cttaagcctc aataaagctt gccttgagtg
    14701 cttcaagtag tgtgtgcccg tctgttgtgt gactctggta gctagagatc cctcataccc
    14761 ttttagtcag tgtggaaaat ctctagca
//
