LOCUS       sequence-304550-        6455 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of AURKA without Nter in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6455)
  AUTHORS   Bertolin G, Bulteau AL, Alves-Guerra MC, Burel A, Lavault MT, Gavard
            O, Le Bras S, Gagne JP, Poirier GG, Le Borgne R, Prigent C, Tramier 
            M
  TITLE     Aurora kinase A localises to mitochondria to control organelle 
            dynamics and energy production.
  JOURNAL   eLife. 2018 Aug 2;7. pii: 38111. doi: 10.7554/eLife.38111.
  PUBMED    30070631
REFERENCE   2  (bases 1 to 6455)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "eLife."; 
            date: "2018-08-2"; volume: "7. pii: 38111. doi"; pages: " 
            10.7554/eLife.38111"
COMMENT     Sequence Label: pcDNA 3.1 AURKA deltaNter
FEATURES             Location/Qualifiers
     source          1..6455
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(29..614)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(109..128)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(785..1576,1577..1645))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1408..1427
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1646..1750)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(1825..1844)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        2016..2395
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2396..2599
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2550..2570
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     2644..2663
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        2644..2662
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             3451..3462
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(3830..3847)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    3836..4060
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      4106..4534
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4193..4212)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4403..4424
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4543..4563)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        4548..4877
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4728..4863
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     4790..4809
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             4944..5738
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4998..5017)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     polyA_signal    5912..6033
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5949..5968)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6003..6022
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6082..6098)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6082..6098)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6095..6117)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6106..6122
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6130..6136,6137..6154,6155..6160))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6175..6196
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6313..6330)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc
       61 acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg
      121 cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat
      181 acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt
      241 atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc
      301 agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg
      361 acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg
      421 gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg
      481 gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg
      541 gcaaacaaac caccgctggt agcggttttt ttgtttgcaa gcagcagatt acgcgcagaa
      601 aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg
      661 aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc
      721 ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg
      781 acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat
      841 ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg
      901 gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa
      961 taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca
     1021 tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc
     1081 gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt
     1141 cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa
     1201 aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat
     1261 cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct
     1321 tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga
     1381 gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag
     1441 tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga
     1501 gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca
     1561 ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg
     1621 cgacacggaa atgttgaata ctcatactct tcctttttca atattattga agcatttatc
     1681 agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag
     1741 gggttccgcg cacatttccc cgaaaagtgc cacctgacgt cgacggatcg ggagatctcc
     1801 cgatccccta tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta
     1861 tctgctccct gcttgtgtgt tggaggtcgc tgagtagtgc gcgagcaaaa tttaagctac
     1921 aacaaggcaa ggcttgaccg acaattgcat gaagaatctg cttagggtta ggcgttttgc
     1981 gctgcttcgc gatgtacggg ccagatatac gcgttgacat tgattattga ctagttatta
     2041 atagtaatca attacggggt cattagttca tagcccatat atggagttcc gcgttacata
     2101 acttacggta aatggcccgc ctggctgacc gcccaacgac ccccgcccat tgacgtcaat
     2161 aatgacgtat gttcccatag taacgccaat agggactttc cattgacgtc aatgggtgga
     2221 gtatttacgg taaactgccc acttggcagt acatcaagtg tatcatatgc caagtacgcc
     2281 ccctattgac gtcaatgacg gtaaatggcc cgcctggcat tatgcccagt acatgacctt
     2341 atgggacttt cctacttggc agtacatcta cgtattagtc atcgctatta ccatggtgat
     2401 gcggttttgg cagtacatca atgggcgtgg atagcggttt gactcacggg gatttccaag
     2461 tctccacccc attgacgtca atgggagttt gttttggcac caaaatcaac gggactttcc
     2521 aaaatgtcgt aacaactccg ccccattgac gcaaatgggc ggtaggcgtg tacggtggga
     2581 ggtctatata agcagagctc tctggctaac tagagaaccc actgcttact ggcttatcga
     2641 aattaatacg actcactata gggagaccca agctggctag cgtttaaact taagcttatg
     2701 tcaaattctt cccagcgcat tcctttgcaa gcacaaaaac ttgtctccag tcacaagccg
     2761 gttcagaatc agaagcagaa gcaattgcag gcaaccagtg tacctcatcc tgtctccagg
     2821 ccactgaata acacccaaaa gagcaagcag cccctgccat cggcacctga aaataatcct
     2881 gaggaggaac tggcatcaaa acagaaaaat gaagaatcaa aaaagaggca gtgggctttg
     2941 gaagactttg aaattggtcg ccctctgggt aaaggaaagt ttggtaatgt ttatttggca
     3001 agagaaaagc aaagcaagtt tattctggct cttaaagtgt tatttaaagc tcagctggag
     3061 aaagccggag tggagcatca gctcagaaga gaagtagaaa tacagtccca ccttcggcat
     3121 cctaatattc ttagactgta tggttatttc catgatgcta ccagagtcta cctaattctg
     3181 gaatatgcac cacttggaac agtttataga gaacttcaga aactttcaaa gtttgatgag
     3241 cagagaactg ctacttatat aacagaattg gcaaatgcac tatcatattg ccattcgaag
     3301 agagttattc atagagacat taagccagag aacttacttc ttggatcagc tggagagctt
     3361 aaaattgcag attttgggtg gtcagtacat gctccatctt ccaggaggac cactctctgt
     3421 ggcaccctgg actacctgcc ccctgaaatg attgaaggtc ggatgcatga tgagaaggtg
     3481 gatctctgga gccttggagt tctttgctat gaatttttag ttgggaagcc tccttttgag
     3541 gcaaacacat accaagagac ctacaaaaga atatcacggg ttgagttcac attccctgac
     3601 tttgtaacag agggagccag ggacctcatt tcaagactgt tgaagcataa tcccagccag
     3661 aggccaatgc tcagagaagt acttgaacac ccctggatca cagcaaattc atcaaaacca
     3721 tcaaattgcc aaaacaaaga atcagctagc aaacagtctg aattctgcag atatccagca
     3781 cagtggcggc cgctcgagtc tagagggccc gtttaaaccc gctgatcagc ctcgactgtg
     3841 ccttctagtt gccagccatc tgttgtttgc ccctcccccg tgccttcctt gaccctggaa
     3901 ggtgccactc ccactgtcct ttcctaataa aatgaggaaa ttgcatcgca ttgtctgagt
     3961 aggtgtcatt ctattctggg gggtggggtg gggcaggaca gcaaggggga ggattgggaa
     4021 gacaatagca ggcatgctgg ggatgcggtg ggctctatgg cttctgaggc ggaaagaacc
     4081 agctggggct ctagggggta tccccacgcg ccctgtagcg gcgcattaag cgcggcgggt
     4141 gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc
     4201 gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg
     4261 gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat
     4321 tagggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg
     4381 ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaaccct
     4441 atctcggtct attcttttga tttataaggg attttgccga tttcggccta ttggttaaaa
     4501 aatgagctga tttaacaaaa atttaacgcg aattaattct gtggaatgtg tgtcagttag
     4561 ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt
     4621 agtcagcaac caggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca
     4681 tgcatctcaa ttagtcagca accatagtcc cgcccctaac tccgcccatc ccgcccctaa
     4741 ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag
     4801 aggccgaggc cgcctctgcc tctgagctat tccagaagta gtgaggaggc ttttttggag
     4861 gcctaggctt ttgcaaaaag ctcccgggag cttgtatatc cattttcgga tctgatcaag
     4921 agacaggatg aggatcgttt cgcatgattg aacaagatgg attgcacgca ggttctccgg
     4981 ccgcttgggt ggagaggcta ttcggctatg actgggcaca acagacaatc ggctgctctg
     5041 atgccgccgt gttccggctg tcagcgcagg ggcgcccggt tctttttgtc aagaccgacc
     5101 tgtccggtgc cctgaatgaa ctgcaggacg aggcagcgcg gctatcgtgg ctggccacga
     5161 cgggcgttcc ttgcgcagct gtgctcgacg ttgtcactga agcgggaagg gactggctgc
     5221 tattgggcga agtgccgggg caggatctcc tgtcatctca ccttgctcct gccgagaaag
     5281 tatccatcat ggctgatgca atgcggcggc tgcatacgct tgatccggct acctgcccat
     5341 tcgaccacca agcgaaacat cgcatcgagc gagcacgtac tcggatggaa gccggtcttg
     5401 tcgatcagga tgatctggac gaagagcatc aggggctcgc gccagccgaa ctgttcgcca
     5461 ggctcaaggc gcgcatgccc gacggcgagg atctcgtcgt gacccatggc gatgcctgct
     5521 tgccgaatat catggtggaa aatggccgct tttctggatt catcgactgt ggccggctgg
     5581 gtgtggcgga ccgctatcag gacatagcgt tggctacccg tgatattgct gaagagcttg
     5641 gcggcgaatg ggctgaccgc ttcctcgtgc tttacggtat cgccgctccc gattcgcagc
     5701 gcatcgcctt ctatcgcctt cttgacgagt tcttctgagc gggactctgg ggttcgaaat
     5761 gaccgaccaa gcgacgccca acctgccatc acgagatttc gattccaccg ccgccttcta
     5821 tgaaaggttg ggcttcggaa tcgttttccg ggacgccggc tggatgatcc tccagcgcgg
     5881 ggatctcatg ctggagttct tcgcccaccc caacttgttt attgcagctt ataatggtta
     5941 caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag
     6001 ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tgtataccgt cgacctctag
     6061 ctagagcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac
     6121 aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt
     6181 gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc
     6241 gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg
     6301 ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt
     6361 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa
     6421 gaacatgtga gcaaaaggcc agcaaaaggc cagga
//
