LOCUS       sequence-304562-        6667 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of AuroraA kinase biosensor under CMV promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6667)
  AUTHORS   Bertolin G, Bulteau AL, Alves-Guerra MC, Burel A, Lavault MT, Gavard
            O, Le Bras S, Gagne JP, Poirier GG, Le Borgne R, Prigent C, Tramier 
            M
  TITLE     Aurora kinase A localises to mitochondria to control organelle 
            dynamics and energy production.
  JOURNAL   eLife. 2018 Aug 2;7. pii: 38111. doi: 10.7554/eLife.38111.
  PUBMED    30070631
REFERENCE   2  (bases 1 to 6667)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "eLife."; 
            date: "2018-08-2"; volume: "7. pii: 38111. doi"; pages: " 
            10.7554/eLife.38111"
COMMENT     Sequence Label: pCMV ShadowY-AURKA-mTurq2
FEATURES             Location/Qualifiers
     source          1..6667
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      36..171
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             220..1014
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             220..1014
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(274..293)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     complement(1202..1221)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    1246..1293
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      1622..2210
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2111..2130
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     enhancer        2385..2688
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2689..2892
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2843..2863
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     misc_feature    3660..3725
                     /label=MCS
                     /note="multiple cloning site"
     CDS             4641..4652
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             join(4971..4973,4974..4976,4977..5489)
                     /codon_start=1
                     /product="N-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN173
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLSWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYFSDNVYITADKQKNGIK
                     ANFKIRHNIE"
     polyA_signal    5779..5900
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5816..5835)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5870..5889
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(5907..6362)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6044..6065)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     6256..6275
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        6389..6493
                     /gene="bla"
                     /label=AmpR promoter
     promoter        join(6495..6667,1..185)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        join(6495..6667,1..19)
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(6518..6538)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
ORIGIN
        1 agtcagcaac catagtcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt
       61 ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg
      121 cctcggcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc ctaggctttt
      181 gcaaagatcg atcaagagac aggatgagga tcgtttcgca tgattgaaca agatggattg
      241 cacgcaggtt ctccggccgc ttgggtggag aggctattcg gctatgactg ggcacaacag
      301 acaatcggct gctctgatgc cgccgtgttc cggctgtcag cgcaggggcg cccggttctt
      361 tttgtcaaga ccgacctgtc cggtgccctg aatgaactgc aagacgaggc agcgcggcta
      421 tcgtggctgg ccacgacggg cgttccttgc gcagctgtgc tcgacgttgt cactgaagcg
      481 ggaagggact ggctgctatt gggcgaagtg ccggggcagg atctcctgtc atctcacctt
      541 gctcctgccg agaaagtatc catcatggct gatgcaatgc ggcggctgca tacgcttgat
      601 ccggctacct gcccattcga ccaccaagcg aaacatcgca tcgagcgagc acgtactcgg
      661 atggaagccg gtcttgtcga tcaggatgat ctggacgaag agcatcaggg gctcgcgcca
      721 gccgaactgt tcgccaggct caaggcgagc atgcccgacg gcgaggatct cgtcgtgacc
      781 catggcgatg cctgcttgcc gaatatcatg gtggaaaatg gccgcttttc tggattcatc
      841 gactgtggcc ggctgggtgt ggcggaccgc tatcaggaca tagcgttggc tacccgtgat
      901 attgctgaag agcttggcgg cgaatgggct gaccgcttcc tcgtgcttta cggtatcgcc
      961 gctcccgatt cgcagcgcat cgccttctat cgccttcttg acgagttctt ctgagcggga
     1021 ctctggggtt cgaaatgacc gaccaagcga cgcccaacct gccatcacga gatttcgatt
     1081 ccaccgccgc cttctatgaa aggttgggct tcggaatcgt tttccgggac gccggctgga
     1141 tgatcctcca gcgcggggat ctcatgctgg agttcttcgc ccaccctagg gggaggctaa
     1201 ctgaaacacg gaaggagaca ataccggaag gaacccgcgc tatgacggca ataaaaagac
     1261 agaataaaac gcacggtgtt gggtcgtttg ttcataaacg cggggttcgg tcccagggct
     1321 ggcactctgt cgatacccca ccgagacccc attggggcca atacgcccgc gtttcttcct
     1381 tttccccacc ccacccccca agttcgggtg aaggcccagg gctcgcagcc aacgtcgggg
     1441 cggcaggccc tgccatagcc tcaggttact catatatact ttagattgat ttaaaacttc
     1501 atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc
     1561 cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt
     1621 cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac
     1681 cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct
     1741 tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact
     1801 tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg
     1861 ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata
     1921 aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga
     1981 cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag
     2041 ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg
     2101 agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac
     2161 ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca
     2221 acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg
     2281 cgttatcccc tgattctgtg gataaccgta ttaccgccat gcattagtta ttaatagtaa
     2341 tcaattacgg ggtcattagt tcatagccca tatatggagt tccgcgttac ataacttacg
     2401 gtaaatggcc cgcctggctg accgcccaac gacccccgcc cattgacgtc aataatgacg
     2461 tatgttccca tagtaacgcc aatagggact ttccattgac gtcaatgggt ggagtattta
     2521 cggtaaactg cccacttggc agtacatcaa gtgtatcata tgccaagtac gccccctatt
     2581 gacgtcaatg acggtaaatg gcccgcctgg cattatgccc agtacatgac cttatgggac
     2641 tttcctactt ggcagtacat ctacgtatta gtcatcgcta ttaccatggt gatgcggttt
     2701 tggcagtaca tcaatgggcg tggatagcgg tttgactcac ggggatttcc aagtctccac
     2761 cccattgacg tcaatgggag tttgttttgg caccaaaatc aacgggactt tccaaaatgt
     2821 cgtaacaact ccgccccatt gacgcaaatg ggcggtaggc gtgtacggtg ggaggtctat
     2881 ataagcagag ctggtttagt gaaccgtcag atccgctagc gctaccggac tcagatctcg
     2941 agatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg gtcgagctgg
     3001 acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc gatgccacct
     3061 acggcaagct gaccctgaag ctcatctgca ccaccggcaa gctgcccgtg ccctggccca
     3121 ccctcgtgac caccttcggc tacggcctga tgtgcttcgc gcgctatcca gaccacatga
     3181 agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag cgcaccatct
     3241 tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag ggcgacaccc
     3301 tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac atcctggggc
     3361 acaagctcga gtacgcttgg cccgtggtga atgtctatat catggccgac aagcagaaga
     3421 acggcatcaa ggtgaacttc tctatctatc ataacatcga ggacggcagc gtgcagctcg
     3481 ccgaccacta ccagcagaac acccccatcg gcgacgggcc cgtgctgctg cccgacaacc
     3541 actacctgag ctactcggct aagctgagca aagaccccaa cgagaagcgc gatcacatgg
     3601 tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag ctgtacaagt
     3661 ccggactcag atctcgagct caagcttcga attctgcagt cgacggtacc gcgggcccgg
     3721 gatccggaag cttcgaattc atggaccgat ctaaagaaaa ctgcatttca ggacctgtta
     3781 aggctacagc tccagttgga ggtccaaaac gtgttctcgt gactcagcaa tttccttgtc
     3841 agaatccatt acctgtaaat agtggccagg ctcagcgggt cttgtgtcct tcaaattctt
     3901 cccagcgcat tcctttgcaa gcacaaaaac ttgtctccag tcacaagccg gttcagaatc
     3961 agaagcagaa gcaattgcag gcaaccagtg tacctcatcc tgtctccagg ccactgaata
     4021 acacccaaaa gagcaagcag cccctgccat cggcacctga aaataatcct gaggaggaac
     4081 tggcatcaaa acagaaaaat gaagaatcaa aaaagaggca gtgggctttg gaagactttg
     4141 aaattggtcg ccctctgggt aaaggaaagt ttggtaatgt ttatttggca agagaaaagc
     4201 aaagcaagtt tattctggct cttaaagtgt tatttaaagc tcagctggag aaagccggag
     4261 tggagcatca gctcagaaga gaagtagaaa tacagtccca ccttcggcat cctaatattc
     4321 ttagactgta tggttatttc catgatgcta ccagagtcta cctaattctg gaatatgcac
     4381 cacttggaac agtttataga gaacttcaga aactttcaaa gtttgatgag cagagaactg
     4441 ctacttatat aacagaattg gcaaatgcac tatcatattg ccattcgaag agagttattc
     4501 atagagacat taagccagag aacttacttc ttggatcagc tggagagctt aaaattgcag
     4561 attttgggtg gtcagtacat gctccatctt ccaggaggac cactctctgt ggcaccctgg
     4621 actacctgcc ccctgaaatg attgaaggtc ggatgcatga tgagaaggtg gatctctgga
     4681 gccttggagt tctttgctat gaatttttag ttgggaagcc tccttttgag gcaaacacat
     4741 accaagagac ctacaaaaga atatcacggg ttgagttcac attccctgac tttgtaacag
     4801 agggagccag ggacctcatt tcaagactgt tgaagcataa tcccagccag aggccaatgc
     4861 tcagagaagt acttgaacac ccctggatca cagcaaattc atcaaaacca tcaaattgcc
     4921 aaaacaaaga atcagctagc aaacagtctg gggatccgat ggcggccgca atggtgagca
     4981 agggcgagga gctgttcacc ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa
     5041 acggccacaa gttcagcgtg tccggcgagg gcgagggcga tgccacctac ggcaagctga
     5101 ccctgaagtt catctgcacc accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca
     5161 ccctgtcctg gggcgtgcag tgcttcgccc gctaccccga ccacatgaag cagcacgact
     5221 tcttcaagtc cgccatgccc gaaggctacg tccaggagcg caccatcttc ttcaaggacg
     5281 acggcaacta caagacccgc gccgaggtga agttcgaggg cgacaccctg gtgaaccgca
     5341 tcgagctgaa gggcatcgac ttcaaggagg acggcaacat cctggggcac aagctggagt
     5401 acaactactt tagcgacaac gtctatatca ccgccgacaa gcagaagaac ggcatcaagg
     5461 ccaacttcaa gatccgccac aacatcgagg acggcggcgt gcagctcgcc gaccactacc
     5521 agcagaacac ccccatcggc gacggccccg tgctgctgcc cgacaaccac tacctgagca
     5581 cccagtccaa gctgagcaaa gaccccaacg agaagcgcga tcacatggtc ctgctggagt
     5641 tcgtgaccgc cgcctaagcg gccgcgactc tagatcataa tcagccatac cacatttgta
     5701 gaggttttac ttgctttaaa aaacctccca cacctccccc tgaacctgaa acataaaatg
     5761 aatgcaattg ttgttgttaa cttgtttatt gcagcttata atggttacaa ataaagcaat
     5821 agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc
     5881 aaactcatca atgtatctta aggcgtaaat tgtaagcgtt aatattttgt taaaattcgc
     5941 gttaaatttt tgttaaatca gctcattttt taaccaatag gccgaaatcg gcaaaatccc
     6001 ttataaatca aaagaataga ccgagatagg gttgagtgtt gttccagttt ggaacaagag
     6061 tccactatta aagaacgtgg actccaacgt caaagggcga aaaaccgtct atcagggcga
     6121 tggcccacta cgtgaaccat caccctaatc aagttttttg gggtcgaggt gccgtaaagc
     6181 actaaatcgg aaccctaaag ggagcccccg atttagagct tgacggggaa agccggcgaa
     6241 cgtggcgaga aaggaaggga agaaagcgaa aggagcgggc gctagggcgc tggcaagtgt
     6301 agcggtcacg ctgcgcgtaa ccaccacacc cgccgcgctt aatgcgccgc tacagggcgc
     6361 gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat
     6421 acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg
     6481 aaaaaggaag agtcctgagg cggaaagaac cagctgtgga atgtgtgtca gttagggtgt
     6541 ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca
     6601 gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat
     6661 ctcaatt
//
