LOCUS       sequence-304584-        3741 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway-compatible Entry vector, with a fragmented insert of S 
            gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3741)
  AUTHORS   Kim DK, Knapp JJ, Kuang D, Chawla A, Cassonnet P, Lee H, 
            Sheykhkarimli D, Samavarchi-Tehrani P, Abdouni H, Rayhan A, Li R, 
            Pogoutse O, Coyaud E, van der Werf S, Demeret C, Gingras AC, Taipale
            M, Raught B, Jacob Y, Roth FP
  TITLE     A Comprehensive, Flexible Collection of SARS-CoV-2 Coding Regions.
  JOURNAL   G3 (Bethesda). 2020 Aug 6. pii: g3.120.401554. doi: 
            10.1534/g3.120.401554.
  PUBMED    32763951
REFERENCE   2  (bases 1 to 3741)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "G3 
            (Bethesda). 2020 Aug 6. pii: g3.120.401554. doi: 
            10.1534/g3.120.401554."
COMMENT     Sequence Label: pDONR221 SARS-CoV-2 S-frag6_nostop
FEATURES             Location/Qualifiers
     source          1..3741
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(127..154)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(246..332)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     381..403
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     395..412
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     396..412
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    428..527
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1725..1824)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1841..1860)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1842..1860)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1865..1881)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1865..1881)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1983..2002)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             1994..2803
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(2061..2080)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      2950..3538
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3439..3458
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3692..3709
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 tctccccgcg cgttggccga ttcattaatg cagctggcac gacaggtttc ccgactggaa
       61 agcgggcagt gagcgcaacg caattaatac gcgtaccgct agccaggaag agtttgtaga
      121 aacgcaaaaa ggccatccgt caggatggcc ttctgcttag tttgatgcct ggcagtttat
      181 ggcgggcgtc ctgcccgcca ccctccgggc cgttgcttca caacgttcaa atccgctccc
      241 ggcggatttg tcctactcag gagagcgttc accgacaaac aacagataaa acgaaaggcc
      301 cagtcttccg actgagcctt tcgttttatt tgatgcctgg cagttcccta ctctcgcgtt
      361 aacgctagca tggatgtttt cccagtcacg acgttgtaaa acgacggcca gtcttaagct
      421 cgggccccaa ataatgattt tattttgact gatagtgacc tgttcgttgc aacaaattga
      481 tgagcaatgc ttttttataa tgccaacttt gtacaaaaaa gttggcatgt ctttcatcga
      541 agacctgctg ttcaacaagg tgaccctggc cgacgccggt ttcatcaagc agtacggtga
      601 ctgcctggga gacatcgccg ctagagacct gatctgcgcc cagaagttca acggtctgac
      661 cgtgctgcca ccactgctga ccgacgagat gatcgcccag tacacctctg ccctgctggc
      721 tggaaccatc acctctggtt ggaccttcgg agctggcgcc gccctgcaga tcccattcgc
      781 tatgcagatg gcctaccgtt tcaacggtat cggagtgacc cagaacgtgc tgtacgaaaa
      841 ccagaagctg atcgctaacc agttcaactc tgctatcgga aagatccagg actccctgtc
      901 ctctaccgct tctgccctgg gaaagctgca ggacgtggtg aaccagaacg cccaggccct
      961 gaacaccctg gtgaagcagc tgtcttctaa cttcggagcc atctcctctg tgctgaacga
     1021 catcctgtct aggctggaca aggtggaagc cgaggtgcag atcgacagac tgatcaccgg
     1081 aagactgcag tctctgcaga cctacgtgac ccagcagctg atcagggccg ctgaaatcag
     1141 agcttctgcc aacctggctg ccaccaagat gtccgaatgc gtgctgggcc agtctaagag
     1201 ggtggacttc tgcggaaagg gataccacct gatgtccttc ccacagtctg ctccacacgg
     1261 agtggtgttc ctgcacgtga cctacgtgcc cgcccaggaa aagaacttca ccaccgcccc
     1321 agctatctgc cacgacggaa aggctcactt ccccagggag ggagtgttcg tgtctaacgg
     1381 aacccactgg ttcgtgaccc agaggaactt ctacgagcca cagatcatca ccaccgacaa
     1441 caccttcgtg tctggaaact gcgacgtggt gatcggaatc gtgaacaaca ccgtgtacga
     1501 cccactgcag ccagaactgg actctttcaa ggaggaactg gacaagtact tcaagaacca
     1561 cacctctcca gacgtggacc tgggagacat ctccggaatc aacgcctctg tggtgaacat
     1621 ccagaaggaa atcgacagac tgaacgaggt ggccaagaac ctgaacgaat ctctgatcga
     1681 cctgcaggaa ctgggaaagt acgagcagta catcaagtgg ccctacccaa ctttcttgta
     1741 caaagttggc attataagaa agcattgctt atcaatttgt tgcaacgaac aggtcactat
     1801 cagtcaaaat aaaatcatta tttgccatcc agctgatatc ccctatagtg agtcgtatta
     1861 catggtcata gctgtttcct ggcagctctg gcccgtgtct caaaatctct gatgttacat
     1921 tgcacaagat aaaaatatat catcatgaac aataaaactg tctgcttaca taaacagtaa
     1981 tacaaggggt gttatgagcc atattcaacg ggaaacgtcg aggccgcgat taaattccaa
     2041 catggatgct gatttatatg ggtataaatg ggctcgcgat aatgtcgggc aatcaggtgc
     2101 gacaatctat cgcttgtatg ggaagcccga tgcgccagag ttgtttctga aacatggcaa
     2161 aggtagcgtt gccaatgatg ttacagatga gatggtcaga ctaaactggc tgacggaatt
     2221 tatgcctctt ccgaccatca agcattttat ccgtactcct gatgatgcat ggttactcac
     2281 cactgcgatc cccggaaaaa cagcattcca ggtattagaa gaatatcctg attcaggtga
     2341 aaatattgtt gatgcgctgg cagtgttcct gcgccggttg cattcgattc ctgtttgtaa
     2401 ttgtcctttt aacagcgatc gcgtatttcg tctcgctcag gcgcaatcac gaatgaataa
     2461 cggtttggtt gatgcgagtg attttgatga cgagcgtaat ggctggcctg ttgaacaagt
     2521 ctggaaagaa atgcataaac ttttgccatt ctcaccggat tcagtcgtca ctcatggtga
     2581 tttctcactt gataacctta tttttgacga ggggaaatta ataggttgta ttgatgttgg
     2641 acgagtcgga atcgcagacc gataccagga tcttgccatc ctatggaact gcctcggtga
     2701 gttttctcct tcattacaga aacggctttt tcaaaaatat ggtattgata atcctgatat
     2761 gaataaattg cagtttcatt tgatgctcga tgagtttttc taatcagaat tggttaattg
     2821 gttgtaacac tggcagagca ttacgctgac ttgacgggac ggcgcaagct catgaccaaa
     2881 atcccttaac gtgagttacg cgtcgttcca ctgagcgtca gaccccgtag aaaagatcaa
     2941 aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc
     3001 accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt
     3061 aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg
     3121 ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc
     3181 agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt
     3241 accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga
     3301 gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct
     3361 tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg
     3421 cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca
     3481 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa
     3541 cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt
     3601 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
     3661 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
     3721 gcgcccaata cgcaaaccgc c
//
