LOCUS       sequence-304590-        7841 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  PiggybacTransposon-based tunable and temporal expression control of 
            SARS-CoV2 N and nuclear eGFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7841)
  TITLE     Inducible COVID19 SARS-CoV2 Nucleocapsid Expressing Plasmid in 
            Mammalian Cells
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7841)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: XLone-Puro SARS-CoV2 N P2A eGFP-NLS
FEATURES             Location/Qualifiers
     source          1..7841
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..368
                     /label=TRE3GS promoter
                     /note="3rd-generation Tet-responsive promoter that can be 
                     activated by binding of Tet-On(R) 3G, modified to eliminate
                     binding sites for endogenous mammalian transcription 
                     factors"
     protein_bind    9..27
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    45..63
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    81..99
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    117..135
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    153..171
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    189..207
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    225..243
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     CDS             1671..1727
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             join(1728..1730,1731..1733,1734..2444)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(1728..1730,1731..1733,1734..2444)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1773..1794)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2034..2053)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2381..2402
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             2445..2465
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    2684..2818
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     repeat_region   3094..3128
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(3139..3156)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3139..3155)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3148..3170)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(3364..3383)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     3483..3505
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(3543..3561)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        3629..3733
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3734..3802,3803..4594)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3952..3971)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4765..5353
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5254..5273
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5507..5524
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    5641..5662
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(5677..5682,5683..5700,5701..5707)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5715..5731
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     5720..5742
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     5739..5755
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     5739..5755
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     repeat_region   5781..5843
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     polyA_signal    6019..6100
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             complement(6121..6720)
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(6204..6224)
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     6701..6720
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     CDS             complement(6730..6783)
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             complement(6793..7536)
                     /codon_start=1
                     /product="modified rtTA protein that binds tightly to 
                     promoters containing the tet operator in the presence of 
                     doxycycline"
                     /label=Tet-On(R) 3G
                     /translation="MSRLDKSKVINSALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHSCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLKQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     CDS             complement(7537..7560)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     regulatory      7560..7569
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(7589..7800)
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
ORIGIN
        1 gagtttactc cctatcagtg atagagaacg tatgaagagt ttactcccta tcagtgatag
       61 agaacgtatg cagactttac tccctatcag tgatagagaa cgtataagga gtttactccc
      121 tatcagtgat agagaacgta tgaccagttt actccctatc agtgatagag aacgtatcta
      181 cagtttactc cctatcagtg atagagaacg tatatccagt ttactcccta tcagtgatag
      241 agaacgtata agctttgctt atgtaaacca gggcgcctat aaaagagtgc tgattttttg
      301 agtaaacttc aattccacaa cacttttgtc ttataccaac tttccgtacc acttcctacc
      361 ctcgtaaagg taccgccacc atgagcgata acggccccca aaaccaacgg aatgcccctc
      421 ggataacatt tggtggccct agtgattcca caggaagtaa ccaaaacgga gaacggtctg
      481 gagcccgctc taaacagagg cgaccacagg gattgccaaa taacacagcg tcatggttta
      541 cagccctgac tcaacatgga aaggaggact tgaaatttcc acgaggacag ggtgtaccaa
      601 taaacacaaa cagtagtcca gacgatcaga tcgggtatta tagaagggcc acgagacgca
      661 tcagaggtgg agatggtaaa atgaaagatc tcagtccgcg ctggtacttt tattatttgg
      721 gaaccggccc agaagcgggc ctcccatatg gagccaataa ggacgggatt atatgggtgg
      781 ctacagaggg agccctcaat acgccaaaag atcacatcgg aacaagaaac cctgctaata
      841 atgccgcaat cgtgctgcag ttgcctcagg ggacgacgct tcctaaaggc ttttacgcag
      901 aaggatcacg cggcggcagc caagcatcct caaggtctag ttccagaagt cgaaacagct
      961 ccagaaactc cacaccaggg tccagtaggg gcacaagtcc ggcgcggatg gcgggcaacg
     1021 gcggagacgc cgcactcgct ctgttgctcc tggaccggct caaccaactt gaatccaaga
     1081 tgagtggtaa gggacagcag caacaaggtc aaaccgtaac caagaaaagc gctgcagaag
     1141 cttctaaaaa acctcgacaa aaacggaccg ctacgaaggc atataacgtt actcaagcct
     1201 tcgggagacg agggccggag caaacccagg ggaattttgg agatcaggag ctcatccggc
     1261 aagggacaga ttacaaacat tggcctcaaa tcgctcagtt cgcgccgtcc gcaagtgctt
     1321 ttttcggaat gtctcgcatc ggtatggaag taacaccatc cgggacatgg ctcacttata
     1381 ccggcgccat caagctggac gacaaagatc caaatttcaa agatcaggtg attctcttga
     1441 ataaacacat agacgcgtat aagacgtttc cacccaccga acccaagaag gacaaaaaga
     1501 aaaaagctga tgaaacacaa gcgttgcccc agcgacagaa gaagcagcaa accgtcaccc
     1561 tcctgccagc cgctgatctc gatgacttct ccaagcaact tcaacagtcc atgagctcag
     1621 ccgattctac tcaggcgctc gaaggcggcg ggggagctag cggcagcggc gccactaact
     1681 tctccctgtt gaaacaagca ggggatgtcg aagagaatcc cgggccaatg gtgagcaagg
     1741 gcgaggagct gttcaccggg gtggtgccca tcctggtcga gctggacggc gacgtaaacg
     1801 gccacaagtt cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc aagctgaccc
     1861 tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc gtgaccaccc
     1921 tgacctacgg cgtgcagtgc ttcagccgct accccgacca catgaagcag cacgacttct
     1981 tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac catcttcttc aaggacgacg
     2041 gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga caccctggtg aaccgcatcg
     2101 agctgaaggg catcgacttc aaggaggacg gcaacatcct ggggcacaag ctggagtaca
     2161 actacaacag ccacaacgtc tatatcatgg ccgacaagca gaagaacggc atcaaggtga
     2221 acttcaagat ccgccacaac atcgaggacg gcagcgtgca gctcgccgac cactaccagc
     2281 agaacacccc catcggcgac ggccccgtgc tgctgcccga caaccactac ctgagcaccc
     2341 agtccgccct gagcaaagac cccaacgaga agcgcgatca catggtcctg ctggagttcg
     2401 tgaccgccgc cgggatcact ctcggcatgg acgagctgta caagccaaag aagaagcgta
     2461 aggtataagt acaagtaaga attccgatca tattcaataa cccactagta gaccacctcc
     2521 cctgcgagct aagctggaca gccaatgacg ggtaagagag tgacattttt cactaaccta
     2581 agacaggagg gccgtcagag ctactgccta atccaaagac gggtaaaagt gataaaaatg
     2641 tatcactcca acctaagaca ggcgcagctt ccgagggatt tgagatccag acatgataag
     2701 atacattgat gagtttggac aaaccaaaac tagaatgcag tgaaaaaaat gccttatttg
     2761 tgaaatttgt gatgctattg ccttatttgt aaccattata agctgcaata aacaagtttg
     2821 atatctataa caagaaaata tatatataat aagttatcac gtaagtagaa catgaaataa
     2881 caatataatt atcgtatgag ttaaatctta aaagtcacgt aaaagataat catgcgtcat
     2941 tttgactcac gcggtcgtta tagttcaaaa tcagtgacac ttaccgcatt gacaagcacg
     3001 cctcacggga gctccaagcg gcgactgaga tgtcctaaat gcacagcgac ggattcgcgc
     3061 tatttagaaa gagagagcaa tatttcaaga atgcatgcgt caattttacg cagactatct
     3121 ttctagggtt aagaattcac tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg
     3181 cgttacccaa cttaatcgcc ttgcagcaca tccccctttc gccagctggc gtaatagcga
     3241 agaggcccgc accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg aatggcgcct
     3301 gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat ggtgcactct
     3361 cagtacaatc tgctctgatg ccgcatagtt aagccagccc cgacacccgc caacacccgc
     3421 tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt
     3481 ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgagacgaaa
     3541 gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac
     3601 gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat
     3661 acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg
     3721 aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc
     3781 attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga
     3841 tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga
     3901 gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg
     3961 cgcggtatta tcccgtattg acgccgggca agagcaactc ggtcgccgca tacactattc
     4021 tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac
     4081 agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact
     4141 tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca
     4201 tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg
     4261 tgacaccacg atgcctgtag caatggcaac aacgttgcgc aaactattaa ctggcgaact
     4321 acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg
     4381 accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg
     4441 tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat
     4501 cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc
     4561 tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat
     4621 actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt
     4681 tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc
     4741 cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt
     4801 gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac
     4861 tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt
     4921 gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct
     4981 gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga
     5041 ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac
     5101 acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg
     5161 agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt
     5221 cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc
     5281 tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg
     5341 gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc
     5401 ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc
     5461 ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag
     5521 cgaggaagcg gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt ggccgattca
     5581 ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat
     5641 taatgtgagt tagctcactc attaggcacc ccaggcttta cactttatgc ttccggctcg
     5701 tatgttgtgt ggaattgtga gcggataaca atttcacaca ggaaacagct atgaccatga
     5761 ttacgccaag gtcgacttaa ccctagaaag ataatcatat tgtgacgtac gttaaagata
     5821 atcatgcgta aaattgacgc atgtgtttta tcggtctgta tatcgaggtt tatttattaa
     5881 tttgaataga tattaagttt tattatattt acacttacat actaataata aattcaacaa
     5941 acaatttatt tatgtttatt tatttattaa aaaaaaacaa aaactcaaaa tttcttctat
     6001 aaagtaacaa aacttttagc agtgaaaaaa atgctttatt tgtgaaattt gtgatgctat
     6061 tgctttattt gtaaccatta taagctgcaa taaacaagtt aactaaacgg gccctctaga
     6121 tcaggcaccg ggcttgcggg tcatgcacca ggtgcgcggt ccttcgggca cctcgacgtc
     6181 ggcggtgacg gtgaagccga gccgctcgta gaaggggagg ttgcggggcg cggaggtctc
     6241 caggaaggcg ggcaccccgg cgcgctcggc cgcctccact ccggggagca cgacggcgct
     6301 gcccagaccc ttgccctggt ggtcgggcga gacgccgacg gtggccagga accacgcggg
     6361 ctccttgggc cggtgcggcg ccaggaggcc ttccatctgt tgctgcgcgg ccagccggga
     6421 accgctcaac tcggccatgc gcgggccgat ctcggcgaac accgcccccg cttcgacgct
     6481 ctccggcgtg gtccagaccg ccaccgcggc gccgtcgtcc gcgacccaca ccttgccgat
     6541 gtcgagcccg acgcgcgtga ggaagagttc ttgcagctcg gtgacccgct cgatgtggcg
     6601 gtccggatcg acggtgtggc gcgtggcggg gtagtcggcg aacgcggcgg cgagggtgcg
     6661 tacggccctg gggacgtcgt cgcgggtggc gaggcgcacc gtgggcttgt actcggtcat
     6721 ctcgagccta gggccgggat tctcctccac gtcaccgcat gttagaagac ttcctctgcc
     6781 ctctccgctg cccccgggga gcatgtcaag gtcaaaatcg tcaagagcgt cagcaggcag
     6841 catatcaagg tcaaagtcgt caagggcatc ggctgggagc atgtctaagt caaaatcgtc
     6901 aagggcgtcg gtcggcccgc cgctttcgca ctttagctgt ttctccaggc cacatatgat
     6961 tagttccagg ccgaaaagga aggcaggttc ggctccctgc cggtcgaaca gctcaattgc
     7021 ttgtttcaga agtgggggca tagaatcggt ggtaggtgtc tctctttcct cttttgctac
     7081 ttgatgctcc tgttcctcca atacgcagcc cagtgtaaag tggcccacgg cggacagagc
     7141 gtacagtgcg ttctccaggg agaagccttg ctgacacagg aacgcgagct gattttccag
     7201 ggtttcgtac tgtttctctg ttgggcgggt gccgagatgc actttagccc cgtcgcgatg
     7261 tgagaggaga gcacagcggt atgacttggc gttgttccgc agaaagtctt gccatgactc
     7321 gccttccagg gggcaggagt gggtatgatg cctgtccagc atctcgattg gcagggcatc
     7381 gagcagggcc cgcttgttct tcacgtgcca gtacagggta ggctgctcaa ctcccagctt
     7441 ttgagcgagt ttccttgtcg tcaggccttc gataccgact ccattgagta attccagagc
     7501 agagtttatg actttgctct tgtccagtct agacatctta tcgtcatcgt ctttgtaatc
     7561 catggtggcg gatcccgcgt cacgacacct gtgttctggc ggcaaacccg ttgcgaaaaa
     7621 gaacgttcac ggcgactact gcacttatat acggttctcc cccaccctcg ggaaaaaggc
     7681 ggagccagta cacgacatca ctttcccagt ttaccccgcg ccaccttctc taggcaccgg
     7741 ttcaattgcc gacccctccc cccaacttct cggggactgt gggcgatgtg cgctctgccc
     7801 actgacgggc accggagcca ctcgagtgga attatcacct c
//
