LOCUS       sequence-304617-         846 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of mEGFP and LANA peptide fusion protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 846)
  AUTHORS   Gibson BA, Doolittle LK, Schneider MWG, Jensen LE, Gamarra N, Henry 
            L, Gerlich DW, Redding S, Rosen MK
  TITLE     Organization of Chromatin by Intrinsic and Regulated Phase 
            Separation.
  JOURNAL   Cell. 2019 Oct 3;179(2):470-484.e21. doi: 
            10.1016/j.cell.2019.08.037. Epub 2019 Sep 19.
  PUBMED    31543265
REFERENCE   2  (bases 1 to 846)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2019.08.037"; journalName: "Cell"; date: "2019-10-3-
            3"; volume: "179"; issue: "2"; pages: "470-484.e21"
COMMENT     Sequence Label: pMTTH+mEGFP-LANA
FEATURES             Location/Qualifiers
     source          1..846
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             join(4..6,7..9,10..720)
                     /codon_start=1
                     /product="enhanced GFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(49..70)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(310..329)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     657..678
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 catatggtga gcaagggcga ggagctgttc accggggtgg tgcctatcct ggtcgagctg
       61 gacggcgacg taaacggcca caagttcagc gtgtccggcg agggcgaggg cgatgccacc
      121 tacggcaagc tgaccctgaa gttcatctgc accaccggca agctgccagt gccgtggcct
      181 accctcgtga ccaccctgac ctacggcgtg cagtgcttca gccgctaccc agaccacatg
      241 aagcagcacg acttcttcaa gtccgccatg ccggaaggct acgtccagga gcgcaccatc
      301 ttcttcaagg acgacggcaa ctacaagacc cgcgccgagg tgaagttcga gggcgacacc
      361 ctggtgaacc gcatcgagct gaagggcatc gacttcaagg aggacggcaa catcctgggg
      421 cacaagctgg agtacaacta caacagccac aacgtctata tcatggccga caagcagaag
      481 aacggcatca aggtgaactt caagatccgc cacaacatcg aggacggcag cgtgcagctc
      541 gccgaccact accagcagaa cacccctatc ggcgacggcc cagtgctgct gccggacaac
      601 cactacctga gcacccagtc caaactgagc aaagacccta acgagaagcg cgatcacatg
      661 gtcctgctgg agttcgtgac cgccgccggg atcactctcg gcatggacga gctgtacaag
      721 ggaggcagtg ggacgggagg tagtggcact ggaggttctg ggactatggc gcctcctggc
      781 atgcgtttgc gttccggccg tagtaccggg gcgccactta cgcgtggatc tggctcgagc
      841 ggatcc
//
