LOCUS       sequence-304622-        2901 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of synthetic protein composed of eGFP and 5 repeats of 
            BRD4 bromodomain #1 with N140A mutation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2901)
  AUTHORS   Gibson BA, Doolittle LK, Schneider MWG, Jensen LE, Gamarra N, Henry 
            L, Gerlich DW, Redding S, Rosen MK
  TITLE     Organization of Chromatin by Intrinsic and Regulated Phase 
            Separation.
  JOURNAL   Cell. 2019 Oct 3;179(2):470-484.e21. doi: 
            10.1016/j.cell.2019.08.037. Epub 2019 Sep 19.
  PUBMED    31543265
REFERENCE   2  (bases 1 to 2901)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2019.08.037"; journalName: "Cell"; date: "2019-10-3-
            3"; volume: "179"; issue: "2"; pages: "470-484.e21"
COMMENT     Sequence Label: pMTTH+eGFP-bromo5(N140A)
FEATURES             Location/Qualifiers
     source          1..2901
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             join(10..12,13..15,16..726)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(10..12,13..15,16..726)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(55..76)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(316..335)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     663..684
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 catatggcca tggtgagcaa gggcgaggag ctgttcaccg gggtggtgcc tatcctggtc
       61 gagctggacg gcgacgtaaa cggccacaag ttcagcgtgt ccggcgaggg cgagggcgat
      121 gccacctacg gcaagctgac cctgaagttc atctgcacca ccggcaagct gccagtgccg
      181 tggcctaccc tcgtgaccac cctgacctac ggcgtgcagt gcttcagccg ctacccagac
      241 cacatgaagc agcacgactt cttcaagtcc gccatgccgg aaggctacgt ccaggagcgc
      301 accatcttct tcaaggacga cggcaactac aagacccgcg ccgaggtgaa gttcgagggc
      361 gacaccctgg tgaaccgcat cgagctgaag ggcatcgact tcaaggagga cggcaacatc
      421 ctggggcaca agctggagta caactacaac agccacaacg tctatatcat ggccgacaag
      481 cagaagaacg gcatcaaggt gaacttcaag atccgccaca acatcgagga cggcagcgtg
      541 cagctcgccg accactacca gcagaacacc cctatcggcg acggcccagt gctgctgccg
      601 gacaaccact acctgagcac ccagtccgcc ctgagcaaag accctaacga gaagcgcgat
      661 cacatggtcc tgctggagtt cgtgaccgcc gccgggatca ctctcggcat ggacgagctg
      721 tacaagggtg gcagcggcgg gtcgggcggg agcggcggca gtggaggaag tgttgcctcg
      781 actaatcctc caccgccgga gaccagtaac ccaaacaagc ctaaacgtca aacgaaccaa
      841 ctccaatatc tgttgcgtgt tgttttgaaa acattgtgga aacaccaatt tgcatggcct
      901 tttcaacaac cggttgacgc ggtgaagtta aaccttccgg attattacaa aattatcaaa
      961 actccgatgg atatggggac tatcaaaaaa cggttggaga acaactacta ctggaatgcc
     1021 caagagtgta ttcaagattt caacaccatg ttcacaaact gttatatcta cgcaaaacca
     1081 ggagacgata tcgttctgat ggccgaggcc cttgagaaat tatttttaca gaaaatcaac
     1141 gaactcccga cagaggaggg cgggtccggt ggaagtggcg gtagtggtgg gagtggtgga
     1201 tcagccgcca gcacaaaccc acctccgcct gaaacgtcca acccgaacaa accgaaacgg
     1261 cagactaacc agctgcagta cttacttcgg gtagtattga agacactgtg gaagcatcag
     1321 ttcgcctggc cgttccaaca gccagtagat gcagttaaac ttaatttacc tgattattat
     1381 aaaatcatta aaacacctat ggatatggga actattaaga aacgcctgga gaataactat
     1441 tattggaatg ctcaagaatg tatccaggat ttcaatacca tgtttacgaa ctgttacatt
     1501 tacgcgaaac ctggtgatga tatcgtgctt atggcagaag cactggaaaa gctgtttttg
     1561 caaaaaatta atgagctgcc tactgaggaa gggggaagcg ggggctcggg ggggtcaggc
     1621 ggttctggag gatctgccgc atccactaac ccgcctccgc cagagacgag taatccgaac
     1681 aagccgaagc gccaaacgaa tcaattacag tatcttcttc gtgtcgtgtt aaaaacgctt
     1741 tggaagcacc agtttgcgtg gccattccag cagcctgtgg atgcggttaa attaaatctc
     1801 ccggactatt acaagatcat caaaacccct atggatatgg gcacgatcaa gaagcgcctc
     1861 gaaaacaatt actattggaa tgcgcaagaa tgcatccagg actttaatac gatgtttact
     1921 aactgctata tttatgctaa accgggcgat gatattgtat tgatggcgga agctcttgaa
     1981 aaattgtttc ttcagaagat caatgaactc cctaccgaag agggaggttc gggagggagc
     2041 ggtggatctg gaggctccgg agggtctgct gcgtctacca atccgccacc accggaaact
     2101 agcaatccta acaaaccaaa acgtcagacc aatcaactgc aatacctctt acgcgttgtg
     2161 cttaaaaccc tctggaaaca ccagtttgcc tggcctttcc agcaaccagt ggacgccgtg
     2221 aagttgaacc tgccagacta ctataagatc attaagacgc caatggatat gggtacaatt
     2281 aagaagcggt tggaaaacaa ctattattgg aatgcacagg aatgtatcca agacttcaat
     2341 actatgttta caaattgcta catctatgcc aagccgggag atgacattgt tttaatggct
     2401 gaggcattag aaaagttgtt cttacaaaag attaatgaat tgcctacgga agaaggtggc
     2461 tctgggggct caggtggtag tgggggttcc gggggctccg cagcctcgac caaccctcct
     2521 ccacctgaga cctctaatcc aaataaacct aagcggcaaa ccaatcagct ccagtacctg
     2581 cttcgcgtag tactgaagac cttatggaaa catcaattcg cttggccgtt tcaacagcct
     2641 gttgatgctg ttaagctgaa cttgcctgat tactacaaga ttatcaagac cccgatggac
     2701 atggggacga ttaaaaagcg gcttgaaaat aattattatt ggaacgccca ggagtgcatt
     2761 caggatttta acacgatgtt caccaattgt tatatttacg caaagccagg ggacgacatc
     2821 gtactcatgg cagaggcact tgagaaactt ttcctccaga aaattaacga gttaccaacc
     2881 gaggagggct cgagcggatc c
//
