LOCUS       sequence-304636-       10117 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector expressing a truncated form of human BHLHE40 (aa 
            1-297).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10117)
  AUTHORS   Emming S, Bianchi N, Polletti S, Balestrieri C, Leoni C, Montagner 
            S, Chirichella M, Delaleu N, Natoli G, Monticelli S
  TITLE     A molecular network regulating the proinflammatory phenotype of 
            human memory T lymphocytes.
  JOURNAL   Nat Immunol. 2020 Apr;21(4):388-399. doi: 10.1038/s41590-020-0622-8.
            Epub 2020 Mar 16.
  PUBMED    32205878
REFERENCE   2  (bases 1 to 10117)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41590-020-0622-8"; journalName: "Nat Immunol"; date: 
            "2020-04"; volume: "21"; issue: "4"; pages: "388-399"
COMMENT     Sequence Label: pLVX-EF1-alpha-BHLHE40 aa1-297-IRES-ZsGreen1
FEATURES             Location/Qualifiers
     source          1..10117
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1283..1855
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    1306..1857
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(1450..1467)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     CDS             1857..2552
                     /codon_start=1
                     /product="Zoanthus green fluorescent protein"
                     /label=ZsGreen1
                     /note="mammalian codon-optimized"
                     /translation="MAQSKHGLTKEMTMKYRMEGCVDGHKFVITGEGIGYPFKGKQAIN
                     LCVVEGGPLPFAEDILSAAFMYGNRVFTEYPQDIVDYFKNSCPAGYTWDRSFLFEDGAV
                     CICNADITVSVEENCMYHESKFYGVNFPADGPVMKKMTDNWEPSCEKIIPVPKQGILKG
                     DVSMYLLLKDGGRLRCQFDTVYKAKSVPRKMPDWHFIQHKLTREDRSDAKNQKWHLTEH
                     AIASGSALP"
     misc_feature    2566..3154
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2619..2639)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3037..3048)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             3361..3994
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(4122..4138)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4122..4138)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4135..4157)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4146..4162
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4170..4176,4177..4194,4195..4200))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4215..4236
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(4353..4370)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4524..5112)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4604..4623)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5283..6074,6075..6143))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5906..5925
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6144..6248)
                     /gene="bla"
                     /label=AmpR promoter
     polyA_signal    6296..6430
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6333..6352)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6387..6406
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             6598..7231
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    7278..7403
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    7901..8134
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8319..8363
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    8626..8741
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1 (lacking the first T)"
     promoter        8935..10117
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          9166..10108
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     10065..10085
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
ORIGIN
        1 ggatctattt ccggtgaatt catggagcgg atccccagcg cgcaaccacc ccccgcctgc
       61 ctgcccaaag caccgggact ggagcacgga gacctaccag ggatgtaccc tgcccacatg
      121 taccaagtgt acaagtcaag acggggaata aagcggagcg aggacagcaa ggagacctac
      181 aaattgccgc accggctcat cgagaaaaag agacgtgacc ggattaacga gtgcatcgcc
      241 cagctgaagg atctcctacc cgaacatctc aaacttacaa ctttgggtca cttggaaaaa
      301 gcagtggttc ttgaacttac cttgaagcat gtgaaagcac taacaaacct aattgatcag
      361 cagcagcaga aaatcattgc cctgcagagt ggtttacaag ctggtgagct gtcagggaga
      421 aatgtcgaaa caggtcaaga gatgttctgc tcaggtttcc agacatgtgc ccgggaggtg
      481 cttcagtatc tggccaagca cgagaacact cgggacctga agtcttcgca gcttgtcacc
      541 cacctccacc gggtggtctc ggagctgctg cagggtggta cctccaggaa gccatcagac
      601 ccagctccca aagtgatgga cttcaaggaa aaacccagct ctccggccaa aggttcggaa
      661 ggtcctggga aaaactgcgt gccagtcatc cagcggactt tcgctcactc gagtggggag
      721 cagagcggca gcgacacgga cacagacagt ggctatggag gagaatcgga gaagggcgac
      781 ttgcgcagtg agcagccgtg cttcaaaagt gaccacggac gcaggttcac gatgggagaa
      841 aggatcggcg caattaagca agagtccgaa gaacccccca caaaaaagaa ccggatgcag
      901 ctttcggatg attaaggcca tttctagagc agtgacctga tcagctcccc gttcctgggc
      961 ccacacccac accagcctcc tttctgcctg cccttctacc tgatcccacc ttcagcgact
     1021 gcctacctgc ccatgctgga gaagtgctgg tatcccacct cagtgccagt gctataccca
     1081 ggcctcaacg cctctgccgc agccctctct agcttcatga acccagacaa gatctcggct
     1141 cccttgctca tgccccagag actcccttct cccttgccag ctcatccgtc cgtcgactct
     1201 tctgtcttgc tccaagctct gaagccaatc ccccctttaa acttagaaac caaagactaa
     1261 tctagagcgg ccgcggatcc cgcccctctc cctccccccc ccctaacgtt actggccgaa
     1321 gccgcttgga ataaggccgg tgtgcgtttg tctatatgtt attttccacc atattgccgt
     1381 cttttggcaa tgtgagggcc cggaaacctg gccctgtctt cttgacgagc attcctaggg
     1441 gtctttcccc tctcgccaaa ggaatgcaag gtctgttgaa tgtcgtgaag gaagcagttc
     1501 ctctggaagc ttcttgaaga caaacaacgt ctgtagcgac cctttgcagg cagcggaacc
     1561 ccccacctgg cgacaggtgc ctctgcggcc aaaagccacg tgtataagat acacctgcaa
     1621 aggcggcaca accccagtgc cacgttgtga gttggatagt tgtggaaaga gtcaaatggc
     1681 tcacctcaag cgtattcaac aaggggctga aggatgccca gaaggtaccc cattgtatgg
     1741 gatctgatct ggggcctcgg tgcacatgct ttacatgtgt ttagtcgagg ttaaaaaacg
     1801 tctaggcccc ccgaaccacg gggacgtggt tttcctttga aaaacacgat gataatatgg
     1861 cccagtccaa gcacggcctg accaaggaga tgaccatgaa gtaccgcatg gagggctgcg
     1921 tggacggcca caagttcgtg atcaccggcg agggcatcgg ctaccccttc aagggcaagc
     1981 aggccatcaa cctgtgcgtg gtggagggcg gccccttgcc cttcgccgag gacatcttgt
     2041 ccgccgcctt catgtacggc aaccgcgtgt tcaccgagta cccccaggac atcgtcgact
     2101 acttcaagaa ctcctgcccc gccggctaca cctgggaccg ctccttcctg ttcgaggacg
     2161 gcgccgtgtg catctgcaac gccgacatca ccgtgagcgt ggaggagaac tgcatgtacc
     2221 acgagtccaa gttctacggc gtgaacttcc ccgccgacgg ccccgtgatg aagaagatga
     2281 ccgacaactg ggagccctcc tgcgagaaga tcatccccgt gcccaagcag ggcatcttga
     2341 agggcgacgt gagcatgtac ctgctgctga aggacggtgg ccgcttgcgc tgccagttcg
     2401 acaccgtgta caaggccaag tccgtgcccc gcaagatgcc cgactggcac ttcatccagc
     2461 acaagctgac ccgcgaggac cgcagcgacg ccaagaacca gaagtggcac ctgaccgagc
     2521 acgccatcgc ctccggctcc gccttgccct gaacgcgtct ggaacaatca acctctggat
     2581 tacaaaattt gtgaaagatt gactggtatt cttaactatg ttgctccttt tacgctatgt
     2641 ggatacgctg ctttaatgcc tttgtatcat gctattgctt cccgtatggc tttcattttc
     2701 tcctccttgt ataaatcctg gttgctgtct ctttatgagg agttgtggcc cgttgtcagg
     2761 caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg gggcattgcc
     2821 accacctgtc agctcctttc cgggactttc gctttccccc tccctattgc cacggcggaa
     2881 ctcatcgccg cctgccttgc ccgctgctgg acaggggctc ggctgttggg cactgacaat
     2941 tccgtggtgt tgtcggggaa gctgacgtcc tttccatggc tgctcgcctg tgttgccacc
     3001 tggattctgc gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc agcggacctt
     3061 ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag
     3121 acgagtcgga tctccctttg ggccgcctcc ccgcctggaa ttaattctgc agtcgagacc
     3181 tagaaaaaca tggagcaatc acaagtagca atacagcagc taccaatgct gattgtgcct
     3241 ggctagaagc acaagaggag gaggaggtgg gttttccagt cacacctcag gtacctttaa
     3301 gaccaatgac ttacaaggca gctgtagatc ttagccactt tttaaaagaa aagaggggac
     3361 tggaagggct aattcactcc caacgaagac aagatatcct tgatctgtgg atctaccaca
     3421 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
     3481 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
     3541 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
     3601 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
     3661 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
     3721 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
     3781 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
     3841 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
     3901 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
     3961 agaccctttt agtcagtgtg gaaaatctct agcagtagta gttcatgtca tcttattatt
     4021 cagtatttat aacttgcaaa gaaatgaata tcagagagtg agaggccttg acattgctag
     4081 cgtttaccgt cgacctctag ctagagcttg gcgtaatcat ggtcatagct gtttcctgtg
     4141 tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa
     4201 gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct
     4261 ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga
     4321 ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc
     4381 gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa
     4441 tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt
     4501 aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa
     4561 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt
     4621 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg
     4681 tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc
     4741 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc
     4801 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta
     4861 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct
     4921 acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc
     4981 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa
     5041 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa
     5101 aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa
     5161 aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt
     5221 ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac
     5281 agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc
     5341 atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt accatctggc
     5401 cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata
     5461 aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc
     5521 cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc
     5581 aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca
     5641 ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa
     5701 gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca
     5761 ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt
     5821 tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt
     5881 tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg
     5941 ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga
     6001 tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc
     6061 agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg
     6121 acacggaaat gttgaatact catactcttc ctttttcaat attattgaag catttatcag
     6181 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg
     6241 gttccgcgca catttccccg aaaagtgcca cctgacgtcg acggatcggg agatcaactt
     6301 gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa
     6361 agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca
     6421 tgtctggatc aactggataa ctcaagctaa ccaaaatcat cccaaacttc ccaccccata
     6481 ccctattacc actgccaatt acctgtggtt tcatttactc taaacctgtg attcctctga
     6541 attattttca ttttaaagaa attgtatttg ttaaatatgt actacaaact tagtagttgg
     6601 aagggctaat tcactcccaa agaagacaag atatccttga tctgtggatc taccacacac
     6661 aaggctactt ccctgattag cagaactaca caccagggcc aggggtcaga tatccactga
     6721 cctttggatg gtgctacaag ctagtaccag ttgagccaga taaggtagaa gaggccaata
     6781 aaggagagaa caccagcttg ttacaccctg tgagcctgca tgggatggat gacccggaga
     6841 gagaagtgtt agagtggagg tttgacagcc gcctagcatt tcatcacgtg gcccgagagc
     6901 tgcatccgga gtacttcaag aactgctgat atcgagcttg ctacaaggga ctttccgctg
     6961 gggactttcc agggaggcgt ggcctgggcg ggactgggga gtggcgagcc ctcagatcct
     7021 gcatataagc agctgctttt tgcctgtact gggtctctct ggttagacca gatctgagcc
     7081 tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga
     7141 gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga
     7201 cccttttagt cagtgtggaa aatctctagc agtggcgccc gaacagggac ttgaaagcga
     7261 aagggaaacc agaggagctc tctcgacgca ggactcggct tgctgaagcg cgcacggcaa
     7321 gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt gactagcgga ggctagaagg
     7381 agagagatgg gtgcgagagc gtcagtatta agcgggggag aattagatcg cgatgggaaa
     7441 aaaattcggt taaggccagg gggaaagaaa aaatataaat taaaacatat agtatgggca
     7501 agcagggagc tagaacgatt cgcagttaat cctggcctgt tagaaacatc agaaggctgt
     7561 agacaaatac tgggacagct acaaccatcc cttcagacag gatcagaaga acttagatca
     7621 ttatataata cagtagcaac cctctattgt gtgcatcaaa ggatagagat aaaagacacc
     7681 aaggaagctt tagacaagat agaggaagag caaaacaaaa gtaagaccac cgcacagcaa
     7741 gcggccggcc gctgatcttc agacctggag gaggagatat gagggacaat tggagaagtg
     7801 aattatataa atataaagta gtaaaaattg aaccattagg agtagcaccc accaaggcaa
     7861 agagaagagt ggtgcagaga gaaaaaagag cagtgggaat aggagctttg ttccttgggt
     7921 tcttgggagc agcaggaagc actatgggcg cagcgtcaat gacgctgacg gtacaggcca
     7981 gacaattatt gtctggtata gtgcagcagc agaacaattt gctgagggct attgaggcgc
     8041 aacagcatct gttgcaactc acagtctggg gcatcaagca gctccaggca agaatcctgg
     8101 ctgtggaaag atacctaaag gatcaacagc tcctggggat ttggggttgc tctggaaaac
     8161 tcatttgcac cactgctgtg ccttggaatg ctagttggag taataaatct ctggaacaga
     8221 tttggaatca cacgacctgg atggagtggg acagagaaat taacaattac acaagcttaa
     8281 tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa gaatgaacaa gaattattgg
     8341 aattagataa atgggcaagt ttgtggaatt ggtttaacat aacaaattgg ctgtggtata
     8401 taaaattatt cataatgata gtaggaggct tggtaggttt aagaatagtt tttgctgtac
     8461 tttctatagt gaatagagtt aggcagggat attcaccatt atcgtttcag acccacctcc
     8521 caaccccgag gggacccgac aggcccgaag gaatagaaga agaaggtgga gagagagaca
     8581 gagacagatc cattcgatta gtgaacggat ctcgacggta tcgcctttaa aagaaaaggg
     8641 gggattgggg ggtacagtgc aggggaaaga atagtagaca taatagcaac agacatacaa
     8701 actaaagaat tacaaaaaca aattacaaaa attcaaaatt ttcgggttta ttacagggac
     8761 agcagagatc cagtttatcg atgagtaatt catacaaaag gactcgcccc tgccttgggg
     8821 aatcccaggg accgtcgtta aactcccact aacgtagaac ccagagatcg ctgcgttccc
     8881 gccccctcac ccgcccgctc tcgtcatcac tgaggtggag aagagcatgc gtgaggctcc
     8941 ggtgcccgtc agtgggcaga gcgcacatcg cccacagtcc ccgagaagtt ggggggaggg
     9001 gtcggcaatt gaaccggtgc ctagagaagg tggcgcgggg taaactggga aagtgatgtc
     9061 gtgtactggc tccgcctttt tcccgagggt gggggagaac cgtatataag tgcagtagtc
     9121 gccgtgaacg ttctttttcg caacgggttt gccgccagaa cacaggtaag tgccgtgtgt
     9181 ggttcccgcg ggcctggcct ctttacgggt tatggccctt gcgtgccttg aattacttcc
     9241 acgcccctgg ctgcagtacg tgattcttga tcccgagctt cgggttggaa gtgggtggga
     9301 gagttcgagg ccttgcgctt aaggagcccc ttcgcctcgt gcttgagttg aggcctggct
     9361 tgggcgctgg ggccgccgcg tgcgaatctg gtggcacctt cgcgcctgtc tcgctgcttt
     9421 cgataagtct ctagccattt aaaatttttg atgacctgct gcgacgcttt ttttctggca
     9481 agatagtctt gtaaatgcgg gccaagatct gcacactggt atttcggttt ttggggccgc
     9541 gggcggcgac ggggcccgtg cgtcccagcg cacatgttcg gcgaggcggg gcctgcgagc
     9601 gcggccaccg agaatcggac gggggtagtc tcaagctggc cggcctgctc tggtgcctgg
     9661 cctcgcgccg ccgtgtatcg ccccgccctg ggcggcaagg ctggcccggt cggcaccagt
     9721 tgcgtgagcg gaaagatggc cgcttcccgg ccctgctgca gggagctcaa aatggaggac
     9781 gcggcgctcg ggagagcggg cgggtgagtc acccacacaa aggaaaaggg cctttccgtc
     9841 ctcagccgtc gcttcatgtg actccacgga gtaccgggcg ccgtccaggc acctcgatta
     9901 gttctcgagc ttttggagta cgtcgtcttt aggttggggg gaggggtttt atgcgatgga
     9961 gtttccccac actgagtggg tggagactga agttaggcca gcttggcact tgatgtaatt
    10021 ctccttggaa tttgcccttt ttgagtttgg atcttggttc attctcaagc ctcagacagt
    10081 ggttcaaagt ttttttcttc catttcaggt gtcgtga
//
