LOCUS       sequence-304637-        7907 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Human IL-22 3'UTR region cloned downstream of luciferase reporter 
            gene.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7907)
  AUTHORS   Emming S, Bianchi N, Polletti S, Balestrieri C, Leoni C, Montagner 
            S, Chirichella M, Delaleu N, Natoli G, Monticelli S
  TITLE     A molecular network regulating the proinflammatory phenotype of 
            human memory T lymphocytes.
  JOURNAL   Nat Immunol. 2020 Apr;21(4):388-399. doi: 10.1038/s41590-020-0622-8.
            Epub 2020 Mar 16.
  PUBMED    32205878
REFERENCE   2  (bases 1 to 7907)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41590-020-0622-8"; journalName: "Nat Immunol"; date: 
            "2020-04"; volume: "21"; issue: "4"; pages: "388-399"
COMMENT     Sequence Label: pmirGLO-3'UTR-IL22
FEATURES             Location/Qualifiers
     source          1..7907
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(49..548)
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(97..116)
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     primer_bind     490..513
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     misc_feature    605..888
                     /label=cer region
                     /note="ColE1-derived recombination site that helps to 
                     maintain plasmids as monomers"
     rep_origin      1004..1592
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1493..1512
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1750..2541,2542..2610))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2373..2392
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2611..2715)
                     /gene="bla"
                     /label=AmpR promoter
     polyA_signal    2871..2919
                     /note="synthetic polyadenylation signal"
     CDS             complement(2983..3774)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="IEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRPV
                     LFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLSS
                     HLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQG
                     LAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIAL
                     ATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(3094..3113)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     3704..3723
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     CDS             complement(3826..4758)
                     /codon_start=1
                     /product="Renilla luciferase"
                     /label=hRluc
                     /note="human codon-optimized"
                     /translation="MASKVYDPEQRKRMITGPQWWARCKQMNVLDSFINYYDSEKHAEN
                     AVIFLHGNAASSYLWRHVVPHIEPVARCIIPDLIGMGKSGKSGNGSYRLLDHYKYLTAW
                     FELLNLPKKIIFVGHDWGACLAFHYSYEHQDKIKAIVHAESVVDVIESWDEWPDIEEDI
                     ALIKSEEGEKMVLENNFFVETMLPSKIMRKLEPEEFAAYLEPFKEKGEVRRPTLSWPRE
                     IPLVKGGKPDVVQIVRNYNAYLRASDDLPKMFIESDPGFFSNAIVEGAKKFPNTEFVKV
                     KGLHFSQEDAPDEMGKYIKSFVERVLKNEQ"
     regulatory      4755..4764
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(4809..5166)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4823..4958
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     enhancer        4975..5166
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     5123..5143
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     polyA_signal    5411..5532
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5448..5467)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5502..5521
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     CDS             complement(join(6257..7907,1..2))
                     /codon_start=1
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="synthetic luc2 version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     regulatory      join(7906..7907,1..8)
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 atggtggctt taccaacagt accggattgc caagcttggg ctgcaggtcg aaaggcccgg
       61 agatgaggaa gaggagaaca gcgcggcaga cgtgcgcttt tgaagcgtgc agaatgccgg
      121 gcctccggag gaccttcggg cgcccgcccc gcccctgagc ccgcccctga gcccgccccc
      181 ggacccaccc cttcccagcc tctgagccca gaaagcgaag gagcaaagct gctattggcc
      241 gctgccccaa aggcctaccc gcttccattg ctcagcggtg ctgtccatct gcacgagact
      301 agtgagacgt gctacttcca tttgtcacgt cctgcacgac gcgagctgcg gggcgggggg
      361 gaacttcctg actaggggag gagtagaagg tggcgcgaag gggccaccaa agaacggagc
      421 cggttggcgc ctaccggtgg atgtggaatg tgtgcgaggc cagaggccac ttgtgtagcg
      481 ccaagtgccc agcggggctg ctaaagcgca tgctccagac tgccttggga aaagcgcctc
      541 ccctacccgg tagatctcga tcctctacgc cggacgcatc gtggccggag tactgcggtt
      601 tatctcgatg gctacgaggg cagacagtaa gtggatttac cataatccct taattgtacg
      661 caccgctaaa acgcgttcag cgcgatcacg gcagcagaca ggtaaaaatg gcaacaaacc
      721 acccgaaaaa ctgccgcaat cgcgcctgat aaattttaac cgtatgaata cctatgcaac
      781 cagagggtac aggccacatt acccccactt aatccactga agctgccatt tttcatggtt
      841 tcaccatccc agcgaagggc catccagcgt gcgttcctgt atttccggac tataaaagga
      901 tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt
      961 tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgaaat cctttttttc
     1021 tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc
     1081 cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac
     1141 caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac
     1201 cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt
     1261 cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct
     1321 gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat
     1381 acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt
     1441 atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg
     1501 cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt
     1561 gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt
     1621 tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg
     1681 tggataaccg tattaccgcc tttgagtgag ctgataccgg ttgggcgtcg cttggtcggt
     1741 catttcgaat taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg
     1801 ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc
     1861 atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc
     1921 agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc
     1981 ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag
     2041 tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat
     2101 ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg
     2161 caaaaaagcg gttagctcct tcggtcctcc gatagttgtc agaagtaagt tggccgcagt
     2221 gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag
     2281 atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg
     2341 accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt
     2401 gaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct
     2461 gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac
     2521 tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat
     2581 aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat
     2641 ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca
     2701 aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtctaag aaaccattac
     2761 gtagaaagcc agtccgcaga aacggtgctg accccggatg aatgtcagct actgggctat
     2821 ctggacaagg gaaaacgcaa gcgcaaagag gatccttatc gctatcgatt cacacaaaaa
     2881 accaacacac agatgtaatg aaaataaaga tattttattg cggccatcgt gatggcaggt
     2941 tgggcgtcgc ttggtcggtc atttcgaacc ccagagtccc gctcagaaga actcgtcaag
     3001 aaggcgatag aaggcgatgc gctgcgaatc gggagcggcg ataccgtaaa gcacgaggaa
     3061 gcggtcagcc cattcgccgc caagctcttc agcaatatca cgggtagcca acgctatgtc
     3121 ctgatagcgg tccgccacac ccagccggcc acagtcgatg aatccagaaa agcggccatt
     3181 ttccaccatg atattcggca agcaggcatc gccatgggtc acgacgagat cctcgccgtc
     3241 gggcatgcgc gccttgagcc tggcgaacag ttcggctggc gcgagcccct gatgctcttc
     3301 gtccagatca tcctgatcga caagaccggc ttccatccga gtacgtgctc gctcgatgcg
     3361 atgtttcgct tggtggtcga atgggcaggt agccggatca agcgtatgca gccgccgcat
     3421 tgcatcagcc atgatggata ctttctcggc aggagcaagg tgagatgaca ggagatcctg
     3481 ccccggcact tcgcccaata gcagccagtc ccttcccgct tcagtgacaa cgtcgagcac
     3541 agctgcgcaa ggaacgcccg tcgtggccag ccacgatagc cgcgctgcct cgtcctgcag
     3601 ttcattcagg gcaccggaca ggtcggtctt gacaaaaaga accgggcgcc cctgcgctga
     3661 cagccggaac acggcggcat cagagcagcc gattgtctgt tgtgcccagt catagccgaa
     3721 tagcctctcc acccaagcgg ccggagaacc tgcgtgcaat ccatcttgtt caatccctcc
     3781 ggagcctccg ccacctgatc cgccacctcc gctcccacca ccggtctgct cgttcttcag
     3841 cacgcgctcc acgaagctct tgatgtactt acccatttca tctggagcgt cctcctggct
     3901 gaagtggagg cccttcacct tcacgaactc ggtgttaggg aacttcttag ctccctcgac
     3961 aatagcgttg gaaaagaacc cagggtcgga ctcgatgaac atcttaggca gatcgtcgct
     4021 ggcccgaagg taggcgttgt agttgcggac aatctggacg acgtcgggct tgcctccctt
     4081 aacgagaggg atctcgcgag gccaggagag ggtaggccgt ctaacctcgc ccttctcctt
     4141 gaatggctcc aggtaggcag cgaactcctc aggctccagt ttccgcatga tcttgcttgg
     4201 gagcatggtc tcgacgaaga agttattctc aagcaccatt ttctcgccct cttcgctctt
     4261 gatcagggcg atatcctcct cgatgtcagg ccactcgtcc caggactcga tcacgtccac
     4321 gacactctca gcatggacga tggccttgat cttgtcttgg tgctcgtagg agtagtgaaa
     4381 ggccagacaa gccccccagt cgtggcccac aaagatgatt ttctttggaa ggttcagcag
     4441 ctcgaaccaa gcggtgaggt acttgtagtg atccaggagg cgatatgagc cattcccgct
     4501 cttgccggac ttacccattc cgatcagatc agggatgatg catctagcca cgggctcgat
     4561 gtgaggcacg acgtgcctcc acaggtagct ggaggcagcg ttaccatgca gaaaaatcac
     4621 ggcgttctcg gcgtgcttct cggaatcata gtagttgatg aaggagtcca gcacgttcat
     4681 ttgcttgcag cgagcccacc actgaggccc agtgatcatg cgtttgcgtt gctcggggtc
     4741 gtacaccttg gaagccatgg tggctccagc ctttggttcg agactgttgt gtcagaagaa
     4801 tcaagctttt tgcaaaagcc taggcctcca aaaaagcctc ctcactactt ctggaatagc
     4861 tcagaggccg aggcggcctc ggcctctgca taaataaaaa aaattagtca gccatggggc
     4921 ggagaatggg cggaactggg cggagttagg ggcgggatgg gcggagttag gggcgggact
     4981 atggttgctg actaattgag atgcatgctt tgcatacttc tgcctgctgg ggagcctggg
     5041 gactttccac acctggttgc tgactaattg agatgcatgc tttgcatact tctgcctgct
     5101 ggggagcctg gggactttcc acaccctaac tgacacacat tccacagctg gttctttccg
     5161 cctcagaagg tacctaacca agttcctctt tcagaggtta tttcaggcca tggtgctgcg
     5221 cagatccgcg tatgcggtgt gaaataccgc acagatgcgt aaggagaaaa taccgcatca
     5281 ggaaattgta agcgttaata ttttgttaaa attcgatttt accacatttg tagaggtttt
     5341 acttgcttaa aaaaacctcc cacatctccc cctgaacctg aaacataaaa tgaatgcaat
     5401 tgttgttgtt aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac
     5461 aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat
     5521 caatgtatct tatcatgtct gctcgaagcg gccgccccaa ggggttatgc tagttattgc
     5581 tcagcggtgg cagcagccaa ctcagcttcc tttcgggctt tgttagcagc cggatcagct
     5641 tgcatgcctg caggtcgact ctagattagg atatccaagt gtttattgag gtcaaataaa
     5701 catgttatag ctctataatt attgtcataa atatcaatat tagccttaca ctcaagtagc
     5761 aatatcgaat gatgtttcta taaataaatc catattaata aaatgatata aataaaatgc
     5821 agtcttataa taataataaa tacatttata aatataaagt actgattatg gaaacatgaa
     5881 gctatttagg ggttttttcc cctaaaggaa tggaaagtaa tcttttttat ggaaaaagac
     5941 aattatttaa aaaataaatt gttttctgtg tatagaacac cagttacaat gaaatgttat
     6001 caataaatat ctatgcttag aaagtctacc ttctggtctt ataaacaaaa gtggcattgg
     6061 tttcctttgt aactaacgca ggggttcatt tggaatccac ccatcatgat ggagtttggc
     6121 ttcccatctt ccttttggtt aaaaaaaatc gctttggggc atctaattgt tatttctagc
     6181 agggaaaggg ggttagttat tcatttttca gctttgctct gggaattcgc tagcgagctc
     6241 gtttaaacaa ctagaattac acggcgatct tgccgccctt cttggcctta atgagaatct
     6301 cgcggatctt gcgggcgtcc aacttgccgg tcagtccttt aggcacctcg tccacgaaca
     6361 caacaccacc gcgcagcttc ttggcggttg taacctggct ggccacatag tccacgatct
     6421 ccttctcggt catggtttta ccgtgttcca gcacgacgac tgcggcgggc agctcgccgg
     6481 catcgtcgtc gggcaggccg gcgaccccgg cgtcgaagat gttggggtgt tgcagcagga
     6541 tgctctccag ttcggctggg gctacctggt agcccttgta tttgatcagg ctcttcagcc
     6601 ggtccacgat gaagaagtgc tcgtcctcgt cccagtaggc gatgtcgccg ctgtgcagcc
     6661 agccgtcctt gtcgatgaga gcgtttgtag cctcggggtt gttaacgtag ccgctcatga
     6721 tcatggggcc acggacgcac agctcgccgc gctggttcac acccagtgtc ttaccggtgt
     6781 ccaagtccac caccttagcc tcgaagaagg gcaccacctt gcctactgcg ccaggcttgt
     6841 cgtccccttc gggggtgatc agaatggcgc tggttgtttc tgtcaggccg tagccctggc
     6901 ggatgcctgg taggtggaag cgtttggcca cggcctcacc tacctccttg ctgagcggcg
     6961 ccccgccgct ggcgatctcg tgcaagttgc ttaggtcgta cttgtcgatg agagtgctct
     7021 tagcgaagaa gctaaatagt gtgggcacca gcagggcaga ttgaatctta tagtcttgca
     7081 agctgcgcaa gaatagctcc tcctcgaagc ggtacatgag cacgacccga aagccgcaga
     7141 tcaagtagcc cagcgtggtg aacatgccga agccgtggtg aaatggcacc acgctgagga
     7201 tagcggtgtc ggggatgatc tggttgccga agatggggtc gcgggcatga ctgaatcgga
     7261 cacaagcggt gcggtgcggt agggctacgc ccttgggcaa tccggtactg ccactactgt
     7321 tcatgatcag ggcgatggtt ttgtcccggt cgaagctctc gggcacgaag tcgtactcgt
     7381 tgaagccggg tggcaaatgg gaagtcacga aggtgtacat gctttggaag ccctggtagt
     7441 cggtcttgct atccatgatg atgatctttt gtatgatcgg tagcttcttt tgcacgttga
     7501 ggatcttttg cagccctttc ttgctcacga atacgacggt gggctggctg atgcccatgc
     7561 tgttcagcag ctcgcgctcg ttgtagatgt cgttagctgg ggccacagcc acaccgatga
     7621 acagggcacc caacacgggc atgaagaact gcaagctatt ctcgctgcac accacgatcc
     7681 gatggtttgt attcagccca tagcgcttca tagcttctgc cagccgaacg ctcatctcga
     7741 agtactcggc gtaggtaatg tccacctcga tatgtgcgtc ggtaaaggcg atggtgccgg
     7801 gcaccagggc gtagcgcttc atggctttgt gcagctgctc gccggcggtc ccgtcttcga
     7861 gtgggtagaa tggcgctggg cccttcttaa tgtttttggc atcttcc
//
