LOCUS       sequence-304649-        5142 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses Borg3 (Cdc42ep5) in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5142)
  AUTHORS   Joberty G, Perlungher RR, Sheffield PJ, Kinoshita M, Noda M, 
            Haystead T, Macara IG
  TITLE     Borg proteins control septin organization and are negatively 
            regulated by Cdc42.
  JOURNAL   Nat Cell Biol. 2001 Oct;3(10):861-6. doi: 10.1038/ncb1001-861.
  PUBMED    11584266
REFERENCE   2  (bases 1 to 5142)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nat Cell Biol"; date: "2001-10"; volume: "3"; issue: "10"; pages: 
            "861-6"
COMMENT     Sequence Label: pKmyc-Borg3
FEATURES             Location/Qualifiers
     source          1..5142
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        14..393
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        394..597
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     548..568
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     594..618
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        828..846
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     828..845
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     859..878
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     CDS             933..962
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     regulatory      1442..1451
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     polyA_signal    1455..1589
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1492..1511)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1546..1565
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     promoter        1658..2015
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        1658..1849
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(1681..1701)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      1866..2001
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(2035..2052)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(2035..2051)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2044..2066)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      2264..2719
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2351..2370)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2561..2582
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(2736..2755)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     2855..2877
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(2915..2933)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        3001..3105
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3106..3174,3175..3966)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3324..3343)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4137..4725
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4626..4645
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4879..4896
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    5013..5034
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(5049..5054,5055..5072,5073..5079)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5087..5103
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     5092..5114
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     5111..5127
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     5111..5127
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 ttcgagctcg cccgacattg attattgact agttattaat agtaatcaat tacggggtca
       61 ttagttcata gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct
      121 ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta
      181 acgccaatag ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac
      241 ttggcagtac atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt
      301 aaatggcccg cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag
      361 tacatctacg tattagtcat cgctattacc atggtgatgc ggttttggca gtacatcaat
      421 gggcgtggat agcggtttga ctcacgggga tttccaagtc tccaccccat tgacgtcaat
      481 gggagtttgt tttggcacca aaatcaacgg gactttccaa aatgtcgtaa caactccgcc
      541 ccattgacgc aaatgggcgg taggcgtgta cggtgggagg tctatataag cagagctcgt
      601 ttagtgaacc gtcagatcgc ctggagacgc catccacgct gttttgacct ccatagaaga
      661 caccgggacc gatccagcct ccgcggccgg gaacggtgca ttggaacgcg gattccccgt
      721 gccaagagtg acgtaagtac cgcctataga gtctataggc ccaccccctt ggcttcgtta
      781 gaacgcggct acaattaata cataacctta tgtatcatac acatacgatt taggtgacac
      841 tatagaataa catccacttt gcctttctct ccacaggtgt ccactcccag gtccaactgc
      901 acctcggttc taagcttctg caggtcgaca tggaacagaa actcatctct gaagaggatc
      961 tgtctagagg atccatgccg gtaatgaagc agctgggccc tgcacaaccc aagaagcggc
     1021 tggatcgcgg agcactctcg atctcagcac ccctcggaga cttcaggcac acattgcacg
     1081 tgggacgcgg tggcgacgcc ttcggggaca cctcattcct gagtcgccac gggggcggtc
     1141 cgcctcccga gcctggagcc ccaccggttg tggccccaca ttctgtcgct ccacctgcag
     1201 ccccacagcc ccctgtggct gtgccttcac ctgcggaccc gctgctgtct ttccacctgg
     1261 acctgggccc ctctatgctg gacgcggtgt taggcgtcat ggacgcggag cgcagcgaga
     1321 caacagccac caagcccgat ggggatgccc accctagagt gcagcacccc aagacccgct
     1381 gctgctcaaa tgctgacctc cagctggacg acgtcatcgg tctgtaggaa ttcaatcgat
     1441 ggccgccatg gcccaacttg tttattgcag cttataatgg ttacaaataa agcaatagca
     1501 tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac
     1561 tcatcaatgt atcttatcat gtctggatcg ggaattaatt cggcgcagca ccatggcctg
     1621 aaataacctc tgaaagagga acttggttag gtaccttctg aggcggaaag aaccagctgt
     1681 ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc
     1741 aaagcatgca tctcaattag tcagcaacca ggtgtggaaa gtccccaggc tccccagcag
     1801 gcagaagtat gcaaagcatg catctcaatt agtcagcaac catagtcccg cccctaactc
     1861 cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa
     1921 ttttttttat ttatgcagag gccgaggccg cctcggcctc tgagctattc cagaagtagt
     1981 gaggaggctt ttttggaggc ctaggctttt gcaaaaagct gttaacagct tggcactggc
     2041 cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt acccaactta atcgccttgc
     2101 agcacatccc cctttcgcca gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc
     2161 ccaacagttg cgcagcctga atggcgaatg gcgcctgatg cggtattttc tccttacgca
     2221 tctgtgcggt atttcacacc gcatacgtca aagcaaccat agtacgcgcc ctgtagcggc
     2281 gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc
     2341 ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc
     2401 cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc
     2461 gaccccaaaa aacttgattt gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg
     2521 gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact
     2581 ggaacaacac tcaaccctat ctcgggctat tcttttgatt tataagggat tttgccgatt
     2641 tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa
     2701 atattaacgt ttacaatttt atggtgcact ctcagtacaa tctgctctga tgccgcatag
     2761 ttaagccagc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc
     2821 ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt
     2881 tcaccgtcat caccgaaacg cgcgagacga aagggcctcg tgatacgcct atttttatag
     2941 gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg
     3001 cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga
     3061 caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat
     3121 ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca
     3181 gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc
     3241 gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca
     3301 atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtat tgacgccggg
     3361 caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca
     3421 gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata
     3481 accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag
     3541 ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg
     3601 gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca
     3661 acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta
     3721 atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct
     3781 ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca
     3841 gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag
     3901 gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat
     3961 tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt
     4021 taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa
     4081 cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga
     4141 gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg
     4201 gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc
     4261 agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag
     4321 aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc
     4381 agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg
     4441 cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac
     4501 accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga
     4561 aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt
     4621 ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag
     4681 cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg
     4741 gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta
     4801 tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc
     4861 agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cccaatacgc
     4921 aaaccgcctc tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc
     4981 gactggaaag cgggcagtga gcgcaacgca attaatgtga gttagctcac tcattaggca
     5041 ccccaggctt tacactttat gcttccggct cgtatgttgt gtggaattgt gagcggataa
     5101 caatttcaca caggaaacag ctatgacatg attacgaatt aa
//
