LOCUS       sequence-304669-        3735 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway-compatible Entry vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3735)
  AUTHORS   Kim DK, Knapp JJ, Kuang D, Chawla A, Cassonnet P, Lee H, 
            Sheykhkarimli D, Samavarchi-Tehrani P, Abdouni H, Rayhan A, Li R, 
            Pogoutse O, Coyaud E, van der Werf S, Demeret C, Gingras AC, Taipale
            M, Raught B, Jacob Y, Roth FP
  TITLE     A Comprehensive, Flexible Collection of SARS-CoV-2 Coding Regions.
  JOURNAL   G3 (Bethesda). 2020 Aug 6. pii: g3.120.401554. doi: 
            10.1534/g3.120.401554.
  PUBMED    32763951
REFERENCE   2  (bases 1 to 3735)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "G3 
            (Bethesda). 2020 Aug 6. pii: g3.120.401554. doi: 
            10.1534/g3.120.401554."
COMMENT     Sequence Label: pDONR223 SARS-CoV2 TEV-NSP5
FEATURES             Location/Qualifiers
     source          1..3735
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(75..102)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(194..280)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     329..351
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     343..360
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     344..360
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    376..475
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             475..495
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     protein_bind    complement(1421..1520)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1537..1556)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1538..1556)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1561..1577)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1561..1577)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             2008..2799
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      2892..3480
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3381..3400
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3634..3651
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 gactggaaag cgggcagtga gcgcaacgca attaatacgc gtaccgctag ccaggaagag
       61 tttgtagaaa cgcaaaaagg ccatccgtca ggatggcctt ctgcttagtt tgatgcctgg
      121 cagtttatgg cgggcgtcct gcccgccacc ctccgggccg ttgcttcaca acgttcaaat
      181 ccgctcccgg cggatttgtc ctactcagga gagcgttcac cgacaaacaa cagataaaac
      241 gaaaggccca gtcttccgac tgagcctttc gttttatttg atgcctggca gttccctact
      301 ctcgcgttaa cgctagcatg gatgttttcc cagtcacgac gttgtaaaac gacggccagt
      361 cttaagctcg ggccccaaat aatgatttta ttttgactga tagtgacctg ttcgttgcaa
      421 caaattgatg agcaatgctt ttttataatg ccaactttgt acaaaaaagt tggcgaaaac
      481 ctgtacttcc aaggcatgtc cggattccgc aagatggcct tcccttctgg caaggtggag
      541 ggctgcatgg tgcaggtgac ctgcggaacc accaccctga acggtctgtg gctggacgac
      601 gtggtgtact gccccaggca cgtgatctgc acctctgagg acatgctgaa ccctaactac
      661 gaagacctgc tgatcagaaa gagcaaccac aacttcctgg tgcaggccgg taacgtgcag
      721 ctgcgtgtga tcggccactc tatgcagaac tgcgtgctga agctgaaggt ggacaccgct
      781 aaccccaaga cccctaagta caagttcgtg agaatccagc ctggtcagac cttctccgtg
      841 ctggcctgct acaacggaag cccatccggc gtgtaccagt gcgctatgcg tcccaacttc
      901 accatcaagg gcagcttcct gaacggctct tgcggaagcg tgggtttcaa catcgactac
      961 gactgcgtgt ccttctgcta catgcaccac atggagctgc caactggagt gcacgctgga
     1021 actgacctgg agggtaactt ctacggcccc ttcgtggaca gacagactgc tcaggctgct
     1081 ggcaccgaca ccaccatcac cgtgaacgtg ctggcttggc tgtacgccgc tgtgatcaac
     1141 ggagaccgct ggttcctgaa caggttcacc accaccctga acgacttcaa cctggtggct
     1201 atgaagtaca actacgagcc tctgacccag gaccacgtgg atatcctggg tccactgagc
     1261 gcccagactg gaatcgctgt gctggacatg tgcgcctccc tgaaggaact gctgcagaac
     1321 ggcatgaacg gacgcaccat cctgggatcc gctctgctgg aggacgaatt cacccccttc
     1381 gacgtggtga ggcagtgctc tggtgtgacc ttccagtaat acccaacttt cttgtacaaa
     1441 gttggcatta taagaaagca ttgcttatca atttgttgca acgaacaggt cactatcagt
     1501 caaaataaaa tcattatttg ccatccagct gatatcccct atagtgagtc gtattacatg
     1561 gtcatagctg tttcctggca gctctggccc gtgtctcaaa atctctgatg ttacattgca
     1621 caagataaaa atatatcatc atgcctcctc tagaccagcc aggacagaaa tgcctcgact
     1681 tcgctgctac ccaaggttgc cgggtgacgc acaccgtgga aacggatgaa ggcacgaacc
     1741 cagtggacat aagcctgttc ggttcgtaag ctgtaatgca agtagcgtat gcgctcacgc
     1801 aactggtcca gaaccttgac cgaacgcagc ggtggtaacg gcgcagtggc ggttttcatg
     1861 gcttgttatg actgtttttt tggggtacag tctatgcctc gggcatccaa gcagcaagcg
     1921 cgttacgccg tgggtcgatg tttgatgtta tggagcagca acgatgttac gcagcagggc
     1981 agtcgcccta aaacaaagtt aaacattatg agggaagcgg tgatcgccga agtatcgact
     2041 caactatcag aggtagttgg cgtcatcgag cgccatctcg aaccgacgtt gctggccgta
     2101 catttgtacg gctccgcagt ggatggcggc ctgaagccac acagtgatat tgatttgctg
     2161 gttacggtga ccgtaaggct tgatgaaaca acgcggcgag ctttgatcaa cgaccttttg
     2221 gaaacttcgg cttcccctgg agagagcgag attctccgcg ctgtagaagt caccattgtt
     2281 gtgcacgacg acatcattcc gtggcgttat ccagctaagc gcgaactgca atttggagaa
     2341 tggcagcgca atgacattct tgcaggtatc ttcgagccag ccacgatcga cattgatctg
     2401 gctatcttgc tgacaaaagc aagagaacat agcgttgcct tggtaggtcc agcggcggag
     2461 gaactctttg atccggttcc tgaacaggat ctatttgagg cgctaaatga aaccttaacg
     2521 ctatggaact cgccgcccga ctgggctggc gatgagcgaa atgtagtgct tacgttgtcc
     2581 cgcatttggt acagcgcagt aaccggcaaa atcgcgccga aggatgtcgc tgccgactgg
     2641 gcaatggagc gcctgccggc ccagtatcag cccgtcatac ttgaagctag acaggcttat
     2701 cttggacaag aagaagatcg cttggcctcg cgcgcagatc agttggaaga atttgtccac
     2761 tacgtgaaag gcgagatcac caaggtagtc ggcaaataac cctcgagcca cccatgacca
     2821 aaatccctta acgtgagtta cgcgtcgttc cactgagcgt cagaccccgt agaaaagatc
     2881 aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa
     2941 ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag
     3001 gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta
     3061 ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta
     3121 ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag
     3181 ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg
     3241 gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg
     3301 cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag
     3361 cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc
     3421 cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa
     3481 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg
     3541 ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct
     3601 gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa
     3661 gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgg
     3721 cacgacaggt ttccc
//
