LOCUS       sequence-304676-       14552 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression vector for an inducible 3xflag-tagged 
            NRF2(a1584c_a1586t_a1589g).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14552)
  AUTHORS   Pereira EJ, Burns JS, Lee CY, Marohl T, Calderon D, Wang L, Atkins 
            KA, Wang CC, Janes KA
  TITLE     Sporadic activation of an oxidative stress-dependent NRF2-p53 
            signaling network in breast epithelial spheroids and 
            premalignancies.
  JOURNAL   Sci Signal. 2020 Apr 14;13(627). pii: 13/627/eaba4200. doi: 
            10.1126/scisignal.aba4200.
  PUBMED    32291314
REFERENCE   2  (bases 1 to 14552)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci 
            Signal."; date: "2020-04-14"; volume: "13(627). pii: 
            13/627/eaba4200. doi"; pages: " 10.1126/scisignal.aba4200"
COMMENT     Sequence Label: pSLIK_hygro_3xflag_NRF2(RR)
FEATURES             Location/Qualifiers
     source          1..14552
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        382..580
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             598..778
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    825..950
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1443..1676
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1861..1905
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2203..2320
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    2377..2401
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        2433..2747
                     /label=tight TRE promoter
                     /note="Tet-responsive promoter PTight, consisting of seven 
                     tet operator sequences followed by the minimal CMV 
                     promoter"
     protein_bind    2441..2459
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2477..2495
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2512..2530
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2548..2566
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2584..2602
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2619..2637
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2655..2673
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     2720..2744
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             2756..2821
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     protein_bind    complement(4678..4702)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        4752..5963
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     5800..5819
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     CDS             join(6088..6705,6706..6711,6712..6747,6748..6750,6751..6786,
                     6787..6789,6790..6825,6826..6834)
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     misc_feature    6997..7459
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(7053..7070)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     7280..7299
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             7518..8537
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGYV
                     LRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPET
                     ELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQTV
                     MDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFGD
                     SQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGNF
                     DDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE"
     CDS             7518..8537
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGYV
                     LRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPET
                     ELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQTV
                     MDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFGD
                     SQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGNF
                     DDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE"
     misc_feature    9003..9591
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(9056..9076)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(9474..9485)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(9594..9610)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9595..9611)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             10120..10300
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(10326..10343)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    10332..10556
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      10602..11030
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(10689..10708)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     10899..10920
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(11039..11059)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        11044..11373
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      11224..11359
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     11286..11305
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        11421..11468
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             11487..11861
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    11991..12112
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(12028..12047)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     12082..12101
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(12161..12177)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(12161..12177)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(12174..12196)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    12185..12201
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(12209..12215,12216..12233,12234..12239))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    12254..12275
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(12392..12409)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(12563..13151)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(12643..12662)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(13322..14113,14114..14182))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     13945..13964
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(14183..14287)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(14362..14381)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag cgcgttttgc ctgtactggg
      601 tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg
      661 cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt
      721 gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt
      781 ggcgcccgaa cagggacttg aaagcgaaag ggaaaccaga ggagctctct cgacgcagga
      841 ctcggcttgc tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg agtacgccaa
      901 aaattttgac tagcggaggc tagaaggaga gagatgggtg cgagagcgtc agtattaagc
      961 gggggagaat tagatcgcga tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat
     1021 ataaattaaa acatatagta tgggcaagca gggagctaga acgattcgca gttaatcctg
     1081 gcctgttaga aacatcagaa ggctgtagac aaatactggg acagctacaa ccatcccttc
     1141 agacaggatc agaagaactt agatcattat ataatacagt agcaaccctc tattgtgtgc
     1201 atcaaaggat agagataaaa gacaccaagg aagctttaga caagatagag gaagagcaaa
     1261 acaaaagtaa gaccaccgca cagcaagcgg ccgctgatct tcagacctgg aggaggagat
     1321 atgagggaca attggagaag tgaattatat aaatataaag tagtaaaaat tgaaccatta
     1381 ggagtagcac ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag agcagtggga
     1441 ataggagctt tgttccttgg gttcttggga gcagcaggaa gcactatggg cgcagcgtca
     1501 atgacgctga cggtacaggc cagacaatta ttgtctggta tagtgcagca gcagaacaat
     1561 ttgctgaggg ctattgaggc gcaacagcat ctgttgcaac tcacagtctg gggcatcaag
     1621 cagctccagg caagaatcct ggctgtggaa agatacctaa aggatcaaca gctcctgggg
     1681 atttggggtt gctctggaaa actcatttgc accactgctg tgccttggaa tgctagttgg
     1741 agtaataaat ctctggaaca gatttggaat cacacgacct ggatggagtg ggacagagaa
     1801 attaacaatt acacaagctt aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa
     1861 aagaatgaac aagaattatt ggaattagat aaatgggcaa gtttgtggaa ttggtttaac
     1921 ataacaaatt ggctgtggta tataaaatta ttcataatga tagtaggagg cttggtaggt
     1981 ttaagaatag tttttgctgt actttctata gtgaatagag ttaggcaggg atattcacca
     2041 ttatcgtttc agacccacct cccaaccccg aggggacccg acaggcccga aggaatagaa
     2101 gaagaaggtg gagagagaga cagagacaga tccattcgat tagtgaacgg atcggcactg
     2161 cgtgcgccaa ttctgcagac aaatggcagt attcatccac aattttaaaa gaaaaggggg
     2221 gattgggggg tacagtgcag gggaaagaat agtagacata atagcaacag acatacaaac
     2281 taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt cgggtttatt acagggacag
     2341 cagagatcca gtttggttaa ttctagacag ttatcaacaa gtttgtacaa aaaagcaggc
     2401 tttaaaggaa ccaattcagt cgactggatc ccgagtttac tccctatcag tgatagagaa
     2461 cgtatgtcga gtttactccc tatcagtgat agagaacgat gtcgagttta ctccctatca
     2521 gtgatagaga acgtatgtcg agtttactcc ctatcagtga tagagaacgt atgtcgagtt
     2581 tactccctat cagtgataga gaacgtatgt cgagtttatc cctatcagtg atagagaacg
     2641 tatgtcgagt ttactcccta tcagtgatag agaacgtatg tcgaggtagg cgtgtacggt
     2701 gggaggccta tataagcaga gctcgtttag tgaaccgtca gatcgccact agatggacta
     2761 caaagaccat gacggtgatt ataaagatca tgacatcgat tacaaggatg acgatgacaa
     2821 gactagtatg gacttggagc tgccgccgcc gggactcccg tcccagcagg acatggattt
     2881 gattgacata ctttggaggc aagatataga tcttggagta agtcgagaag tatttgactt
     2941 cagtcagcga cggaaagagt atgagctgga aaaacagaaa aaacttgaaa aggaaagaca
     3001 agaacaactc caaaaggagc aagagaaagc ctttttcgct cagttacaac tagatgaaga
     3061 gacaggtgaa tttctcccaa ttcagccagc ccagcacatc cagtcagaaa ccagtggatc
     3121 tgccaactac tcccaggttg cccacattcc caaatcagat gctttgtact ttgatgactg
     3181 catgcagctt ttggcgcaga cattcccgtt tgtagatgac aatgaggttt cttcggctac
     3241 gtttcagtca cttgttcctg atattcccgg tcacatcgag agcccagtct tcattgctac
     3301 taatcaggct cagtcacctg aaacttctgt tgctcaggta gcccctgttg atttagacgg
     3361 tatgcaacag gacattgagc aagtttggga ggagctatta tccattcctg agttacagtg
     3421 tcttaatatt gaaaatgaca agctggttga gactaccatg gttccaagtc cagaagccaa
     3481 actgacagaa gttgacaatt atcattttta ctcatctata ccctcaatgg aaaaagaagt
     3541 aggtaactgt agtccacatt ttcttaatgc ttttgaggat tccttcagca gcatcctctc
     3601 cacagaagac cccaaccagt tgacagtgaa ctcattaaat tcagatgcca cagtcaacac
     3661 agattttggt gatgaatttt attctgcttt catagctgag cccagtatca gcaacagcat
     3721 gccctcacct gctactttaa gccattcact ctctgaactt ctaaatgggc ccattgatgt
     3781 ttctgatcta tcactttgca aagctttcaa ccaaaaccac cctgaaagca cagcagaatt
     3841 caatgattct gactccggca tttcactaaa cacaagtccc agtgtggcat caccagaaca
     3901 ctcagtggaa tcttccagct atggagacac actacttggc ctcagtgatt ctgaagtgga
     3961 agagctagat agtgcccctg gaagtgtcaa acagaatggt cctaaaacac cagtacattc
     4021 ttctggggat atggtacaac ccttgtcacc atctcagggg cagagcactc acgtgcatga
     4081 tgcccaatgt gagaacacac cagagaaaga attgcctgta agtcctggtc atcggaaaac
     4141 cccattcaca aaagacaaac attcaagccg cttggaggct catctcacaa gagatgaact
     4201 tagggcaaaa gctctccata tcccattccc tgtagaaaaa atcattaacc tccctgttgt
     4261 tgacttcaac gaaatgatgt ccaaagagca gttcaatgaa gctcaacttg cattaattcg
     4321 ggatatacgt aggaggggta agaataaagt ggctgctcag aattgcagaa aaagaaaact
     4381 ggaaaatata gtagaactag agcaagattt cgttcgtttg aaagatgaaa aagaaaaatt
     4441 gctcaaagaa aaaggagaaa atgacaaaag ccttcaccta ctgaaaaaac aactcagcac
     4501 cttatatctc gaagttttca gcatgctacg tgatgaagat ggaaaacctt attctcctag
     4561 tgaatactcc ctgcagcaaa caagagatgg caatgttttc cttgttccca aaagtaagaa
     4621 gccagatgtt aagaaaaact agcaattgga agactaatgc gttttcgaga tatctagacc
     4681 cagctttctt gtacaaagtg gttgataaca tctcgagcca taaagatggt taattaaccc
     4741 acccaagatc tggcctccgc gccgggtttt ggcgcctccc gcgggcgccc ccctcctcac
     4801 ggcgagcgct gccacgtcag acgaagggcg cagcgagcgt cctgatcctt ccgcccggac
     4861 gctcaggaca gcggcccgct gctcataaga ctcggcctta gaaccccagt atcagcagaa
     4921 ggacatttta ggacgggact tgggtgactc tagggcactg gttttctttc cagagagcgg
     4981 aacaggcgag gaaaagtagt cccttctcgg cgattctgcg gagggatctc cgtggggcgg
     5041 tgaacgccga tgattatata aggacgcgcc gggtgtggca cagctagttc cgtcgcagcc
     5101 gggatttggg tcgcggttct tgtttgtgga tcgctgtgat cgtcacttgg tgagtagcgg
     5161 gctgctgggc tggccggggc tttcgtggcc gccgggccgc tcggtgggac ggaagcgtgt
     5221 ggagagaccg ccaagggctg tagtctgggt ccgcgagcaa ggttgccctg aactgggggt
     5281 tggggggagc gcagcaaaat ggcggctgtt cccgagtctt gaatggaaga cgcttgtgag
     5341 gcgggctgtg aggtcgttga aacaaggtgg ggggcatggt gggcggcaag aacccaaggt
     5401 cttgaggcct tcgctaatgc gggaaagctc ttattcgggt gagatgggct ggggcaccat
     5461 ctggggaccc tgacgtgaag tttgtcactg actggagaac tcggtttgtc gtctgttgcg
     5521 ggggcggcag ttatggcggt gccgttgggc agtgcacccg tacctttggg agcgcgcgcc
     5581 ctcgtcgtgt cgtgacgtca cccgttctgt tggcttataa tgcagggtgg ggccacctgc
     5641 cggtaggtgt gcggtaggct tttctccgtc gcaggacgca gggttcgggc ctagggtagg
     5701 ctctcctgaa tcgacaggcg ccggacctct ggtgagggga gggataagtg aggcgtcagt
     5761 ttctttggtc ggttttatgt acctatcttc ttaagtagct gaagctccgg ttttgaacta
     5821 tgcgctcggg gttggcgagt gtgttttgtg aagtttttta ggcacctttt gaaatgtaat
     5881 catttgggtc aatatgtaat tttcagtgtt agactagtaa attgtccgct aaattctggc
     5941 cgtttttggc ttttttgtta gacgaagctt ggtaccgagc tcggatctcc accccgtacc
     6001 ggtcctgcag gatttaaatg gcgcgccgct agcgctaccg gactcagatc tcgagctcaa
     6061 gcttcgaatt ctgcagtcga attcaccatg tctagactgg acaagagcaa agtcataaac
     6121 ggagctctgg aattactcaa tggtgtcggt atcgaaggcc tgacgacaag gaaactcgct
     6181 caaaagctgg gagttgagca gcctaccctg tactggcacg tgaagaacaa gcgggccctg
     6241 ctcgatgccc tgccaatcga gatgctggac aggcatcata cccacttctg ccccctggaa
     6301 ggcgagtcat ggcaagactt tctgcggaac aacgccaagt cataccgctg tgctctcctc
     6361 tcacatcgcg acggggctaa agtgcatctc ggcacccgcc caacagagaa acagtacgaa
     6421 accctggaaa atcagctcgc gttcctgtgt cagcaaggct tctccctgga gaacgcactg
     6481 tacgctctgt ccgccgtggg ccactttaca ctgggctgcg tattggagga acaggagcat
     6541 caagtagcaa aagaggaaag agagacacct accaccgatt ctatgccccc acttctgaga
     6601 caagcaattg agctgttcga ccggcaggga gccgaacctg ccttcctttt cggcctggaa
     6661 ctaatcatat gtggcctgga gaaacagcta aagtgcgaaa gcggcgggcc gaccgacgcc
     6721 cttgacgatt ttgacttaga catgctccca gccgatgccc ttgacgactt tgaccttgat
     6781 atgctgcctg ctgacgctct tgacgatttt gaccttgaca tgctccccgg gtaactaagt
     6841 aaggatccgc ggccgcacta gaggaattcc gcccctctcc ccccccccyc ctcnctcccc
     6901 cccccctaac gttactggcc gaagccgctt ggaataaggc cggtgtgcgt ttgtctatat
     6961 gttattttcc accatattgc cgtcttttgg caatgtgagg gcccggaaac ctggccctgt
     7021 cttcttgacg agcattccta ggggtctttc ccctctcgcc aaaggaatgc aaggtctgtt
     7081 gaatgtcgtg aaggaagcag ttcctctgga agcttcttga agacaaacaa cgtctgtagc
     7141 gaccctttgc aggcagcgga accccccacc tggcgacagg tgcctctgcg gccaaaagcc
     7201 acgtgtataa gatacacctg caaaggcggc acaaccccag tgccacgttg tgagttggat
     7261 agttgtggaa agagtcaaat ggctctcctc aagcgtattc aacaaggggc tgaaggatgc
     7321 ccagaaggta ccccattgta tgggatctga tctggggcct cggtgcacat gctttacatg
     7381 tgtttagtcg aggttaaaaa aacgtctagg ccccccgaac cacggggacg tggttttcct
     7441 ttgaaaaaca cgatgataag cttaccggta cgcgtgatag cttgccacaa cccgtaccaa
     7501 agatggatag atccggaaag cctgaactca ccgcgacgtc tgtcgagaag tttctgatcg
     7561 aaaagttcga cagcgtctcc gacctgatgc agctctcgga gggcgaagaa tctcgtgctt
     7621 tcagcttcga tgtaggaggg cgtggatatg tcctgcgggt aaatagctgc gccgatggtt
     7681 tctacaaaga tcgttatgtt tatcggcact ttgcatcggc cgcgctcccg attccggaag
     7741 tgcttgacat tggggaattc agcgagagcc tgacctattg catctcccgc cgtgcacagg
     7801 gtgtcacgtt gcaagacctg cctgaaaccg aactgcccgc tgttctgcag ccggtcgcgg
     7861 aggccatgga tgcgatcgct gcggccgatc ttagccagac gagcgggttc ggcccattcg
     7921 gaccgcaagg aatcggtcaa tacactacat ggcgtgattt catatgcgcg attgctgatc
     7981 cccatgtgta tcactggcaa actgtgatgg acgacaccgt cagtgcgtcc gtcgcgcagg
     8041 ctctcgatga gctgatgctt tgggccgagg actgccccga agtccggcac ctcgtgcacg
     8101 cggatttcgg ctccaacaat gtcctgacgg acaatggccg cataacagcg gtcattgact
     8161 ggagcgaggc gatgttcggg gattcccaat acgaggtcgc caacatcttc ttctggaggc
     8221 cgtggttggc ttgtatggag cagcagacgc gctacttcga gcggaggcat ccggagcttg
     8281 caggatcgcc gcggctccgg gcgtatatgc tccgcattgg tcttgaccaa ctctatcaga
     8341 gcttggttga cggcaatttc gatgatgcag cttgggcgca gggtcgatgc gacgcaatcg
     8401 tccgatccgg agccgggact gtcgggcgta cacaaatcgc ccgcagaagc gcggccgtct
     8461 ggaccgatgg ctgtgtagaa gtactcgccg atagtggaaa ccgacgcccc agcactcgtc
     8521 cgagggcaaa ggaatagagt agatgccgac cgaacaagag ctgatttcga gaacgcctca
     8581 gccagcaact cgcgcgagcc tagcaaggca aatgcgagag aacggcctta cgcttggtgg
     8641 cacagttctc gtccacagtt cgctaagctc gctcggctgg gtcgcgggag ggccggtcgc
     8701 agtgattcag gcccttctgg attgtgttgg tccccagggc acgattgtca tgcccacgca
     8761 ctcgggtgat ctgactgatc ccgcagattg gagatcgccg cccgtgcctg ccgattgggt
     8821 gcagatctct agatctgtac aagtaaagcg gccgcgactc tagatcataa tcagccatac
     8881 cacatttgta gaggttttac ttgctttaaa aaacctccca cacctccccc tgaacctgaa
     8941 acataaaatg aatgcaattg ttgttgttta gtccctccca attcgatatc aagcttatcg
     9001 ataatcaacc tctggattac aaaatttgtg aaagattgac tggtattctt aactatgttg
     9061 ctccttttac gctatgtgga tacgctgctt taatgccttt gtatcatgct attgcttccc
     9121 gtatggcttt cattttctcc tccttgtata aatcctggtt gctgtctctt tatgaggagt
     9181 tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca
     9241 ctggttgggg cattgccacc acctgtcagc tcctttccgg gactttcgct ttccccctcc
     9301 ctattgccac ggcggaactc atcgccgcct gccttgcccg ctgctggaca ggggctcggc
     9361 tgttgggcac tgacaattcc gtggtgttgt cggggaaatc atcgtccttt ccttggctgc
     9421 tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc
     9481 tcaatccagc ggaccttcct tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc
     9541 ttcgccttcg ccctcagacg agtcggatct ccctttgggc cgcctccccg catcgatacc
     9601 gtcgacctcg atcgagacct agaaaaacat ggagcaatca caagtagcaa tacagcagct
     9661 accaatgctg attgtgcctg gctagaagca caagaggagg aggaggtggg ttttccagtc
     9721 acacctcagg tacctttaag accaatgact tacaaggcag ctgtagatct tagccacttt
     9781 ttaaaagaaa aggggggact ggaagggcta attcactccc aacgaagaca agatatcctt
     9841 gatctgtgga tctaccacac acaaggctac ttccctgatt ggcagaacta cacaccaggg
     9901 ccagggatca gatatccact gacctttgga tggtgctaca agctagtacc agttgagcaa
     9961 gagaaggtag aagaagccaa tgaaggagag aacacccgct tgttacaccc tgtgagcctg
    10021 catgggatgg atgacccgga gagagaagta ttagagtgga ggtttgacag ccgcctagca
    10081 tttcatcaca tggcccgaga gctgcatccg gactgtactg ggtctctctg gttagaccag
    10141 atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc
    10201 ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga
    10261 tccctcagac ccttttagtc agtgtggaaa atctctagca gggcccgttt aaacccgctg
    10321 atcagcctcg actgtgcctt ctagttgcca gccatctgtt gtttgcccct cccccgtgcc
    10381 ttccttgacc ctggaaggtg ccactcccac tgtcctttcc taataaaatg aggaaattgc
    10441 atcgcattgt ctgagtaggt gtcattctat tctggggggt ggggtggggc aggacagcaa
    10501 gggggaggat tgggaagaca atagcaggca tgctggggat gcggtgggct ctatggcttc
    10561 tgaggcggaa agaaccagct ggggctctag ggggtatccc cacgcgccct gtagcggcgc
    10621 attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct
    10681 agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg
    10741 tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga
    10801 ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt
    10861 ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg
    10921 aacaacactc aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc
    10981 ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt aattctgtgg
    11041 aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa
    11101 agcatgcatc tcaattagtc agcaaccagg tgtggaaagt ccccaggctc cccagcaggc
    11161 agaagtatgc aaagcatgca tctcaattag tcagcaacca tagtcccgcc cctaactccg
    11221 cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt
    11281 ttttttattt atgcagaggc cgaggccgcc tctgcctctg agctattcca gaagtagtga
    11341 ggaggctttt ttggaggcct aggcttttgc aaaaagctcc cgggagcttg tatatccatt
    11401 ttcggatctg atcagcacgt gttgacaatt aatcatcggc atagtatatc ggcatagtat
    11461 aatacgacaa ggtgaggaac taaaccatgg ccaagttgac cagtgccgtt ccggtgctca
    11521 ccgcgcgcga cgtcgccgga gcggtcgagt tctggaccga ccggctcggg ttctcccggg
    11581 acttcgtgga ggacgacttc gccggtgtgg tccgggacga cgtgaccctg ttcatcagcg
    11641 cggtccagga ccaggtggtg ccggacaaca ccctggcctg ggtgtgggtg cgcggcctgg
    11701 acgagctgta cgccgagtgg tcggaggtcg tgtccacgaa cttccgggac gcctccgggc
    11761 cggccatgac cgagatcggc gagcagccgt gggggcggga gttcgccctg cgcgacccgg
    11821 ccggcaactg cgtgcacttc gtggccgagg agcaggactg acacgtgcta cgagatttcg
    11881 attccaccgc cgccttctat gaaaggttgg gcttcggaat cgttttccgg gacgccggct
    11941 ggatgatcct ccagcgcggg gatctcatgc tggagttctt cgcccacccc aacttgttta
    12001 ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat
    12061 ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct
    12121 gtataccgtc gacctctagc tagagcttgg cgtaatcatg gtcatagctg tttcctgtgt
    12181 gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag
    12241 cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt
    12301 tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag
    12361 gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg
    12421 ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat
    12481 caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta
    12541 aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa
    12601 atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc
    12661 cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt
    12721 ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca
    12781 gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg
    12841 accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat
    12901 cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta
    12961 cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct
    13021 gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac
    13081 aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa
    13141 aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa
    13201 actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt
    13261 taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca
    13321 gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca
    13381 tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc
    13441 ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa
    13501 accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc
    13561 agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca
    13621 acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat
    13681 tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag
    13741 cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac
    13801 tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt
    13861 ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt
    13921 gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc
    13981 tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat
    14041 ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca
    14101 gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga
    14161 cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg
    14221 gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg
    14281 ttccgcgcac atttccccga aaagtgccac ctgacgtcga cggatcggga gatctcccga
    14341 tcccctatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatct
    14401 gctccctgct tgtgtgttgg aggtcgctga gtagtgcgcg agcaaaattt aagctacaac
    14461 aaggcaaggc ttgaccgaca attgcatgaa gaatctgctt agggttaggc gttttgcgct
    14521 gcttcgcgat gtacgggcca gatatacgcg tt
//
