LOCUS       sequence-304694-       10382 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  High purity C-to-T base editor.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10382)
  AUTHORS   Arbab M, Shen MW, Mok B, Wilson C, Matuszek Z, Cassa CA, Liu DR
  TITLE     Determinants of Base Editing Outcomes from Target Library Analysis 
            and Machine Learning.
  JOURNAL   Cell. 2020 Jun 9. pii: S0092-8674(20)30632-2. doi: 
            10.1016/j.cell.2020.05.037.
  PUBMED    32533916
REFERENCE   2  (bases 1 to 10382)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2020 
            Jun 9. pii: S0092-8674(20)30632-2. doi: 10.1016/j.cell.2020.05.037."
COMMENT     Sequence Label: p2T-CMV-eA3A-BE5-NG-BlastR
FEATURES             Location/Qualifiers
     source          1..10382
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     48..67
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        48..66
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             116..136
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             827..4927
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SIRPKRNSDKLIARKKDWDPKKYGGFVSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASARFLQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPRAFKYFDTTIDRKVYRSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             4958..5206
                     /codon_start=1
                     /product="uracil-DNA glycosylase inhibitor from a Bacillus 
                     subtilis bacteriophage (Mol et al., 1995)"
                     /label=UGI
                     /translation="TNLSDIIEKETGKQLVIQESILMLPEEVEEVIGNKPESDILVHTA
                     YDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIKML"
     CDS             5237..5485
                     /codon_start=1
                     /product="uracil-DNA glycosylase inhibitor from a Bacillus 
                     subtilis bacteriophage (Mol et al., 1995)"
                     /label=UGI
                     /translation="TNLSDIIEKETGKQLVIQESILMLPEEVEEVIGNKPESDILVHTA
                     YDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIKML"
     CDS             5528..5548
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             5557..5574
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(5597..5614)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5603..5827
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(5898..5914)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5898..5914)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5911..5933)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5922..5938
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5946..5952,5953..5970,5971..5976))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5991..6012
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     polyA_signal    6129..6250
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6140..6159)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     6194..6213
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(6408..6806)
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     promoter        complement(6825..6872)
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     promoter        complement(6920..7249)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6934..7069
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(6988..7007)
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     primer_bind     7234..7254
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     misc_feature    join(7373..7560,7561..7572)
                     /label=Tol2 left end
                     /note="left end of the Tol2 transposon from the medaka fish
                     Oryzias latipes (Kawakami, 2007)"
     rep_origin      complement(7633..8221)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7713..7732)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8392..9183,9184..9252))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9015..9034
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9253..9357)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(9436..9455)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     misc_feature    join(9647..9658,9659..9796)
                     /label=Tol2 right end
                     /note="right end of the Tol2 transposon from the medaka 
                     fish Oryzias latipes (Kawakami, 2007)"
     enhancer        9805..10184
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        join(10185..10382,1..6)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     10339..10359
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
ORIGIN
        1 agagctggtt tagtgaaccg tcagatccgc tagagatccg cggccgctaa tacgactcac
       61 tatagggaga gccgccacca tgaaacggac agccgacgga agcgagttcg agtcaccaaa
      121 gaagaagcgg aaagtcgagg caagcccagc aagcggccca cggcacctga tggaccccca
      181 cattttcacc agcaacttca acaacggaat cggacgacac aagacctacc tgtgctatga
      241 ggtggagagg ctggacaacg gcgacgccgt gaagatggat cagcacagag gcttcctgca
      301 cggccaggcc aagaatctgc tgtgcggctt ctacggccgg cacgcagagc tgcgctttct
      361 ggacctggtg ccatctctgc agctggatcc cgcccagatc tatagggtga cctggttcat
      421 cagctggtcc ccttgcttta gctggggatg tgcaggagag gtgagggcct tcctgcagga
      481 gaacacacac gtgaggctga gaatctttgc cgcccggatc tacgactatg atcccctgta
      541 caaggaggcc ctgcagatgc tgagggacgc aggagcccag gtgtctatca tgacctatga
      601 tgagttcaag cactgctggg acacatttgt ggatcaccag ggctgtccct ttcagccttg
      661 ggacggcctg gacgagcatt cccaggcact gagcggacgg ctgagggcaa ttctgcagaa
      721 ccagggcaat tctggcggat ctagcggagg atcctctggc agcgagacac caggaacaag
      781 cgagtcagca acaccagaga gcagtggcgg cagcagcggc ggcagcgaca agaagtacag
      841 catcggcctg gccatcggca ccaactctgt gggctgggcc gtgatcaccg acgagtacaa
      901 ggtgcccagc aagaaattca aggtgctggg caacaccgac cggcacagca tcaagaagaa
      961 cctgatcgga gccctgctgt tcgacagcgg cgaaacagcc gaggccaccc ggctgaagag
     1021 aaccgccaga agaagataca ccagacggaa gaaccggatc tgctatctgc aagagatctt
     1081 cagcaacgag atggccaagg tggacgacag cttcttccac agactggaag agtccttcct
     1141 ggtggaagag gataagaagc acgagcggca ccccatcttc ggcaacatcg tggacgaggt
     1201 ggcctaccac gagaagtacc ccaccatcta ccacctgaga aagaaactgg tggacagcac
     1261 cgacaaggcc gacctgcggc tgatctatct ggccctggcc cacatgatca agttccgggg
     1321 ccacttcctg atcgagggcg acctgaaccc cgacaacagc gacgtggaca agctgttcat
     1381 ccagctggtg cagacctaca accagctgtt cgaggaaaac cccatcaacg ccagcggcgt
     1441 ggacgccaag gccatcctgt ctgccagact gagcaagagc agacggctgg aaaatctgat
     1501 cgcccagctg cccggcgaga agaagaatgg cctgttcgga aacctgattg ccctgagcct
     1561 gggcctgacc cccaacttca agagcaactt cgacctggcc gaggatgcca aactgcagct
     1621 gagcaaggac acctacgacg acgacctgga caacctgctg gcccagatcg gcgaccagta
     1681 cgccgacctg tttctggccg ccaagaacct gtccgacgcc atcctgctga gcgacatcct
     1741 gagagtgaac accgagatca ccaaggcccc cctgagcgcc tctatgatca agagatacga
     1801 cgagcaccac caggacctga ccctgctgaa agctctcgtg cggcagcagc tgcctgagaa
     1861 gtacaaagag attttcttcg accagagcaa gaacggctac gccggctaca ttgacggcgg
     1921 agccagccag gaagagttct acaagttcat caagcccatc ctggaaaaga tggacggcac
     1981 cgaggaactg ctcgtgaagc tgaacagaga ggacctgctg cggaagcagc ggaccttcga
     2041 caacggcagc atcccccacc agatccacct gggagagctg cacgccattc tgcggcggca
     2101 ggaagatttt tacccattcc tgaaggacaa ccgggaaaag atcgagaaga tcctgacctt
     2161 ccgcatcccc tactacgtgg gccctctggc caggggaaac agcagattcg cctggatgac
     2221 cagaaagagc gaggaaacca tcaccccctg gaacttcgag gaagtggtgg acaagggcgc
     2281 ttccgcccag agcttcatcg agcggatgac caacttcgat aagaacctgc ccaacgagaa
     2341 ggtgctgccc aagcacagcc tgctgtacga gtacttcacc gtgtataacg agctgaccaa
     2401 agtgaaatac gtgaccgagg gaatgagaaa gcccgccttc ctgagcggcg agcagaaaaa
     2461 ggccatcgtg gacctgctgt tcaagaccaa ccggaaagtg accgtgaagc agctgaaaga
     2521 ggactacttc aagaaaatcg agtgcttcga ctccgtggaa atctccggcg tggaagatcg
     2581 gttcaacgcc tccctgggca cataccacga tctgctgaaa attatcaagg acaaggactt
     2641 cctggacaat gaggaaaacg aggacattct ggaagatatc gtgctgaccc tgacactgtt
     2701 tgaggacaga gagatgatcg aggaacggct gaaaacctat gcccacctgt tcgacgacaa
     2761 agtgatgaag cagctgaagc ggcggagata caccggctgg ggcaggctga gccggaagct
     2821 gatcaacggc atccgggaca agcagtccgg caagacaatc ctggatttcc tgaagtccga
     2881 cggcttcgcc aacagaaact tcatgcagct gatccacgac gacagcctga cctttaaaga
     2941 ggacatccag aaagcccagg tgtccggcca gggcgatagc ctgcacgagc acattgccaa
     3001 tctggccggc agccccgcca ttaagaaggg catcctgcag acagtgaagg tggtggacga
     3061 gctcgtgaaa gtgatgggcc ggcacaagcc cgagaacatc gtgatcgaaa tggccagaga
     3121 gaaccagacc acccagaagg gacagaagaa cagccgcgag agaatgaagc ggatcgaaga
     3181 gggcatcaaa gagctgggca gccagatcct gaaagaacac cccgtggaaa acacccagct
     3241 gcagaacgag aagctgtacc tgtactacct gcagaatggg cgggatatgt acgtggacca
     3301 ggaactggac atcaaccggc tgtccgacta cgatgtggac catatcgtgc ctcagagctt
     3361 tctgaaggac gactccatcg acaacaaggt gctgaccaga agcgacaaga accggggcaa
     3421 gagcgacaac gtgccctccg aagaggtcgt gaagaagatg aagaactact ggcggcagct
     3481 gctgaacgcc aagctgatta cccagagaaa gttcgacaat ctgaccaagg ccgagagagg
     3541 cggcctgagc gaactggata aggccggctt catcaagaga cagctggtgg aaacccggca
     3601 gatcacaaag cacgtggcac agatcctgga ctcccggatg aacactaagt acgacgagaa
     3661 tgacaagctg atccgggaag tgaaagtgat caccctgaag tccaagctgg tgtccgattt
     3721 ccggaaggat ttccagtttt acaaagtgcg cgagatcaac aactaccacc acgcccacga
     3781 cgcctacctg aacgccgtcg tgggaaccgc cctgatcaaa aagtacccta agctggaaag
     3841 cgagttcgtg tacggcgact acaaggtgta cgacgtgcgg aagatgatcg ccaagagcga
     3901 gcaggaaatc ggcaaggcta ccgccaagta cttcttctac agcaacatca tgaacttttt
     3961 caagaccgag attaccctgg ccaacggcga gatccggaag cggcctctga tcgagacaaa
     4021 cggcgaaacc ggggagatcg tgtgggataa gggccgggat tttgccaccg tgcggaaagt
     4081 gctgagcatg ccccaagtga atatcgtgaa aaagaccgag gtgcagacag gcggcttcag
     4141 caaagagtct atccggccca agaggaacag cgataagctg atcgccagaa agaaggactg
     4201 ggaccctaag aagtacggcg gcttcgtcag ccccaccgtg gcctattctg tgctggtggt
     4261 ggccaaagtg gaaaagggca agtccaagaa actgaagagt gtgaaagagc tgctggggat
     4321 caccatcatg gaaagaagca gcttcgagaa gaatcccatc gactttctgg aagccaaggg
     4381 ctacaaagaa gtgaaaaagg acctgatcat caagctgcct aagtactccc tgttcgagct
     4441 ggaaaacggc cggaagagaa tgctggcctc tgcccgcttc ctgcagaagg gaaacgaact
     4501 ggccctgccc tccaaatatg tgaacttcct gtacctggcc agccactatg agaagctgaa
     4561 gggctccccc gaggataatg agcagaaaca gctgtttgtg gaacagcaca agcactacct
     4621 ggacgagatc atcgagcaga tcagcgagtt ctccaagaga gtgatcctgg ccgacgctaa
     4681 tctggacaaa gtgctgtccg cctacaacaa gcaccgggat aagcccatca gagagcaggc
     4741 cgagaatatc atccacctgt ttaccctgac caatctggga gcccctcgcg ccttcaagta
     4801 ctttgacacc accatcgacc ggaaggtgta ccgcagcacc aaagaggtgc tggacgccac
     4861 cctgatccac cagagcatca ccggcctgta cgagacacgg atcgacctgt ctcagctggg
     4921 aggtgacagc ggcgggagcg gcgggagcgg ggggagcact aatctgagcg acatcattga
     4981 gaaggagact gggaaacagc tggtcattca ggagtccatc ctgatgctgc ctgaggaggt
     5041 ggaggaagtg atcggcaaca agccagagtc tgacatcctg gtgcacaccg cctacgacga
     5101 gtccacagat gagaatgtga tgctgctgac ctctgacgcc cccgagtata agccttgggc
     5161 cctggtcatc caggattcta acggcgagaa taagatcaag atgctgagcg gaggatccgg
     5221 aggatctgga ggcagcacca acctgtctga catcatcgag aaggagacag gcaagcagct
     5281 ggtcatccag gagagcatcc tgatgctgcc cgaagaagtc gaagaagtga tcggaaacaa
     5341 gcctgagagc gatatcctgg tccataccgc ctacgacgag agtaccgacg aaaatgtgat
     5401 gctgctgaca tccgacgccc cagagtataa gccctgggct ctggtcatcc aggattccaa
     5461 cggagagaac aaaatcaaaa tgctgtctgg cggctcaaaa agaaccgccg acggcagcga
     5521 attcgagccc aagaagaaga ggaaagtcta accggtcatc atcaccatca ccattgagtt
     5581 taaacccgct gatcagcctc gactgtgcct tctagttgcc agccatctgt tgtttgcccc
     5641 tcccccgtgc cttccttgac cctggaaggt gccactccca ctgtcctttc ctaataaaat
     5701 gaggaaattg catcgcattg tctgagtagg tgtcattcta ttctgggggg tggggtgggg
     5761 caggacagca agggggagga ttgggaagac aatagcaggc atgctgggga tgcggtgggc
     5821 tctatggctt ctgaggcgga aagaaccagc tggggctcga taccgtcgac ctctagctag
     5881 agcttggcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt
     5941 ccacacaaca tacgagccgg aagcataaag tgtaaagcct agggtgccta atgagtgagc
     6001 taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc
     6061 cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctac
     6121 agacatgata agatacattg atgagtttgg acaaaccaca actagaatgc agtgaaaaaa
     6181 atgctttatt tgtgaaattt gtgatgctat tgctttattt gtaaccatta taagctgcaa
     6241 taaacaagtt ggggtgggcg aagaactcca gcatgagatc cccgcgctgg aggatcatcc
     6301 agccggcgtc ccggaaaacg attccgaagc ccaacctttc atagaaggcg gcggtggaat
     6361 cgaaatctcg tagcacgtgt cagtcctgct cctcggccac gaagtgctta gccctcccac
     6421 acataaccag agggcagcaa ttcacgaatc ccaactgccg tcggctgtcc atcactgtcc
     6481 ttcactatgg ctttgatccc aggatgcaga tcgagaagca cctgtcggca ccgtccgcag
     6541 gggctcaaga tgcccctgtt ctcatttccg atcgcgacga tacaagtcag gttgccagct
     6601 gccgcagcag cagcagtgcc cagcaccacg agttctgcac aaggtccccc agtaaaatga
     6661 tatacattga caccagtgaa gatgcggccg tcgctagaga gagctgcgct ggcgacgctg
     6721 tagtcttcag agatggggat gctgttgatt gtagccgttg ctctttcaat gagggtggat
     6781 tcttcttgag acaaaggctt ggccatggtt tagttcctca ccttgtcgta ttatactatg
     6841 ccgatatact atgccgatga ttaattgtca acacgtgctg atcagatccg aaaatggata
     6901 tacaagctcc cgggagcttt ttgcaaaagc ctaggcctcc aaaaaagcct cctcactact
     6961 tctggaatag ctcagaggca gaggcggcct cggcctctgc ataaataaaa aaaattagtc
     7021 agccatgggg cggagaatgg gcggaactgg gcggagttag gggcgggatg ggcggagtta
     7081 ggggcgggac tatggttgct gactaattga gatgcatgct ttgcatactt ctgcctgctg
     7141 gggagcctgg ggactttcca cacctggttg ctgactaatt gagatgcatg ctttgcatac
     7201 ttctgcctgc tggggagcct ggggactttc cacaccctaa ctgacacaca ttccacagaa
     7261 ttcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag
     7321 gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat gtaagtgatc
     7381 tccaaaaaat aagtactttt tgactgtaaa taaaattgta aggagtaaaa agtacttttt
     7441 tttctaaaaa aatgtaatta agtaaaagta aaagtattga tttttaattg tactcaagta
     7501 aagtaaaaat ccccaaaaat aatacttaag tacagtaatc aagtaaaatt actcaagtac
     7561 tttacacctc tgcatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc
     7621 gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc
     7681 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag
     7741 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct
     7801 cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta
     7861 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc
     7921 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc
     7981 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt
     8041 gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct
     8101 gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc
     8161 tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca
     8221 agaagatcct ttgatctttt ctacggggtc tgacactcag tggaacgaaa actcacgtta
     8281 agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa
     8341 atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg
     8401 cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg
     8461 actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc
     8521 aatgataccg cgggacccac gctcaccggc tccagattta tcagcaataa accagccagc
     8581 cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa
     8641 ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc
     8701 cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg
     8761 ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc
     8821 cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat
     8881 ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg
     8941 tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc
     9001 ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg
     9061 aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat
     9121 gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg
     9181 gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg
     9241 ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct
     9301 catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac
     9361 atttccccga aaagtgccac ctgacgtcga cggatcggga gatcgatctc ccgatcccct
     9421 agggtcgact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atctgctccc
     9481 tgcttgtgtg ttggaggtcg ctgagtagtg cgcgagcaaa atttaagcta caacaaggca
     9541 aggcttgacc gacaattgca tgaagaatct gcttagggtt aggcgttttg cgctgcttcg
     9601 cgatgtacgg gccagatata cgcgggtacc ggcatatggt tcttgacaga ggtgtaaaaa
     9661 gtactcaaaa attttactca agtgaaagta caagtactta gggaaaattt tactcaatta
     9721 aaagtaaaag tatctggcta gaatcttact tgagtaaaag taaaaaagta ctccattaaa
     9781 attgtacttg agtattaacg cgttgacatt gattattgac tagttattaa tagtaatcaa
     9841 ttacggggtc attagttcat agcccatata tggagttccg cgttacataa cttacggtaa
     9901 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg
     9961 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt
    10021 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg
    10081 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc
    10141 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc
    10201 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca
    10261 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta
    10321 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa
    10381 gc
//
