LOCUS       sequence-304695-        3078 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  HA-TEV-VFT-TMD-CC-PreX-eGFP in pFastBac1 for Sf9 expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3078)
  AUTHORS   Shaye H, Ishchenko A, Lam JH, Han GW, Xue L, Rondard P, Pin JP, 
            Katritch V, Gati C, Cherezov V
  TITLE     Structural basis of the activation of a metabotropic GABA receptor.
  JOURNAL   Nature. 2020 Jun 17. pii: 10.1038/s41586-020-2408-4. doi: 
            10.1038/s41586-020-2408-4.
  PUBMED    32555460
REFERENCE   2  (bases 1 to 3078)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2020 Jun 17. pii: 10.1038/s41586-020-2408-4. doi: 
            10.1038/s41586-020-2408-4."
COMMENT     Sequence Label: IMPT-10316:GABR1
FEATURES             Location/Qualifiers
     source          1..3078
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             49..69
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             2335..2358
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     CDS             join(2359..2361,2362..2364,2365..3075)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
ORIGIN
        1 atgaaaacaa ttatagctct cagctacata ttttgtctgg tgttcgcgga gaacttgtac
       61 ttccagggat cggaacgccg tgccgtctac atcggagcac tgttcccaat gagtggagga
      121 tggccaggtg gacaggcttg tcaaccagca gtcgagatgg cactggaaga cgttaacagc
      181 aggagagaca tcctcccaga ttacgagctg aaactcattc accatgactc taagtgcgat
      241 cctggtcagg ctaccaagta cctgtacgaa ctgctctaca acgaccctat caagatcatt
      301 ttgatgcccg gctgttcttc agtcagcact ctggttgctg aggctgccag gatgtggaac
      361 ctgatcgtcc tcagttacgg cagttcgtcc cctgcgctgt cgaacaggca gagattccca
      421 accttcttcc gcactcaccc gtcagctacc ttgcataacc ctactcgtgt gaaactgttc
      481 gagaagtggg gatggaagaa aatcgccaca attcagcaaa ccactgaagt cttcacctcc
      541 actttggacg atctggagga aagggttaag gaggcaggta tcgaaattac cttcagacag
      601 tccttcttct ccgaccctgc cgttccggtg aagaacctga aacgccaaga cgcacgtatc
      661 attgtcggtc tcttctacga aaccgaggct cgtaaagtgt tctgcgaggt ctacaaggaa
      721 agactcttcg gcaagaagta cgtgtggttc ttgatcggct ggtacgccga taactggttc
      781 aagatctacg accctagcat taactgtaca gtggacgaaa tgacggaggc cgtcgaaggc
      841 cacatcacaa cggagattgt catgctgaac cccgcaaaca cccgtagtat ctcgaacatg
      901 acttctcagg agttcgtgga aaagctgaca aaacgcctca agcgtcatcc cgaggaaacg
      961 ggaggtttcc aagaggcgcc actggcttac gacgccatct gggcattggc gctggctctc
     1021 aacaagacct ctggcggagg tggccgttca ggtgtcagac tggaagattt caactacaac
     1081 aaccagacaa tcacggacca aatttaccgc gctatgaaca gctcttcatt cgagggtgtg
     1141 tccggccacg ttgtgttcga tgcgtccggc agccgtatgg cttggacttt gatcgaacag
     1201 ctgcaaggag gttcctacaa gaaaattgga tactacgaca gcaccaagga cgatttgtct
     1261 tggtcaaaaa ctgataagtg gatcggcgga tcaccaccgg ctgaccagac cctcgtcatt
     1321 aaaactttca ggttcctctc ccaaaagttg ttcatcagtg tctcggttct cagttcgttg
     1381 ggcattgtcc tcgctgtcgt ttgcttgagt ttcaacatct acaactcgca cgttagatac
     1441 attcagaact ctcaacccaa cctgaacaac ctcaccgccg ttggatgctc attggcactg
     1501 gcagcggtgt tcccgctcgg attggacggt taccatatcg gtcgcaacca gttccccttc
     1561 gtgtgtcaag cccgtctgtg gttgctggga ctgggtttct ccctcggcta cggaagcatg
     1621 ttcactaaga tctggtgggt tcacaccgtg ttcactaaga aagaggaaaa gaaagagtgg
     1681 cgcaaaacac tggaaccatg gaagctctac gcgacggttg gtctcttggt gggcatggat
     1741 gtcctgacac tcgctatctg gcagattgtc gaccctctgc acaggacaat cgagacgttc
     1801 gccaaagagg aaccgaagga agacatcgat gtgtcaattt tgcctcagct ggagcattgc
     1861 tccagcagaa agatgaacac ttggctggga atcttctacg gctacaaggg actgctcttg
     1921 ctgctcggta tcttcctcgc ttacgagaca aaatccgtta gcacggaaaa gatcaacgat
     1981 caccgcgcgg tgggtatggc tatttacaac gtggcggtct tgtgtctgat cacagctcct
     2041 gtgacgatga ttttgtcttc acagcaagac gctgccttcg ccttcgcatc tctggctatc
     2101 gtgttcagtt cgtacattac tctcgtggtc ttgttcgtcc ccaagatgcg ccgtctgatc
     2161 acacgtggcg agtggcagtc agaagcccaa gacaccatga agactggttc cagcaccaac
     2221 aacaacgagg aagagaaatc ccgcttgctg gaaaaggaga accgtgaact ggagaaaatc
     2281 attgctgaga aggaagagag ggtgagcgaa ttgagacacc agctgcaatc ccgcctggag
     2341 gtgctgtttc aggggcctat ggtgagcaag ggagaggagc tgttcaccgg agtggtgcct
     2401 atcctggtgg agctggacgg cgatgtgaat ggccacaagt tttctgtgag cggagaggga
     2461 gagggcgacg caacctatgg caagctgaca ctgaagttca tctgcaccac aggcaagctg
     2521 ccagtgccct ggcctaccct ggtgaccaca ctgacatacg gcgtgcagtg tttctctcgg
     2581 tatccagacc acatgaagca gcacgacttc ttcaagagcg ccatgcccga gggctacgtg
     2641 caggagagga ccatcttttt caaggacgat ggcaactata agacccgcgc cgaggtgaag
     2701 tttgagggcg acacactggt gaaccggatc gagctgaagg gcatcgactt caaggaggat
     2761 ggcaatatcc tgggccacaa gctggagtac aactataata gccacaacgt gtacatcatg
     2821 gccgataagc agaagaacgg catcaaggtg aacttcaaga tccgccacaa tatcgaggac
     2881 ggctccgtgc agctggccga tcactaccag cagaacaccc caatcggcga cggccctgtg
     2941 ctgctgccag ataatcacta tctgtccaca cagtctgccc tgagcaagga ccctaacgag
     3001 aagcgggatc acatggtgct gctggagttc gtgaccgcag caggaatcac actgggaatg
     3061 gacgagctgt ataaataa
//
