LOCUS       sequence-304701-        2475 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  HA-3xFLAG-VFT-TMD-CC in pFastBac1 for Sf9 expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2475)
  AUTHORS   Shaye H, Ishchenko A, Lam JH, Han GW, Xue L, Rondard P, Pin JP, 
            Katritch V, Gati C, Cherezov V
  TITLE     Structural basis of the activation of a metabotropic GABA receptor.
  JOURNAL   Nature. 2020 Jun 17. pii: 10.1038/s41586-020-2408-4. doi: 
            10.1038/s41586-020-2408-4.
  PUBMED    32555460
REFERENCE   2  (bases 1 to 2475)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2020 Jun 17. pii: 10.1038/s41586-020-2408-4. doi: 
            10.1038/s41586-020-2408-4."
COMMENT     Sequence Label: IMPT-10314:GABBR2
FEATURES             Location/Qualifiers
     source          1..2475
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             49..114
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             118..138
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
ORIGIN
        1 atgaaaacaa ttatagctct cagctacata ttttgtctgg tgttcgcgga ttataaggac
       61 cacgatggag actataaaga ccatgacatt gattataagg atgatgacga taaattggag
      121 aacttgtact tccagggatg ggctcgcggt gcaccccgtc ctcccccatc cagcccgcct
      181 ctgtctatca tgggattgat gccactgacc aaggaggtgg ctaaaggttc aatcggaaga
      241 ggagttctgc cagcagtgga gctcgcaatc gaacagattc gcaacgagtc cctgctccgt
      301 ccctacttct tggacctgcg tctctacgac accgaatgcg ataacgccaa gggcctcaaa
      361 gcgttctacg atgctatcaa gtacggaccc aaccacttga tggtcttcgg tggcgtttgt
      421 ccaagtgtga cttcgatcat tgctgagagt ctccagggct ggaacctcgt gcaattgtcg
      481 ttcgctgcca ccactccagt cttggccgac aagaaaaagt acccgtactt cttccgcacc
      541 gtgccttccg ataacgccgt caaccccgca atcctgaagt tgctgaaaca ttaccagtgg
      601 aagcgtgtcg gaaccctcac tcaggacgtt caacgcttca gcgaggtgcg taacgatctg
      661 actggcgtgc tctacggaga ggacatcgaa atttctgata cagaaagttt ctcgaacgac
      721 ccttgcacgt cagtcaaaaa gctgaagggc aacgacgttc gtatcattct cggacagttc
      781 gatcaaaaca tggcagcgaa ggtgttctgc tgtgcatacg aggaaaacat gtacggtagt
      841 aaataccagt ggatcattcc cggctggtac gagccatcgt ggtgggaaca agtccacacc
      901 gaggccaact cttcacgctg tctccgtaag aacctcttgg ctgccatgga aggctacatc
      961 ggagtggact tcgagccgct gagttcgaag cagatcaaaa ccattagcgg aaagactcct
     1021 cagcaatacg agagggaata caacaacaag agatctggtg tcggcccctc aaaattccac
     1081 ggatacgctt acgacggtat ctgggttatt gcgaagacat tgcaaagggc tatggagacg
     1141 ctgcacgcct ccagcaggca tcagagaatc caagacttca actacacaga tcatacgttg
     1201 ggcagaatca ttctgaacgc catgaacgag acaaacttct tcggtgtcac gggccaggtg
     1261 gtcttcagga acggtgaaag aatgggcaca atcaagttca cgcagttcca agactctcgc
     1321 gaagttaaag tgggagagta caacgcagtg gcggacaccc tggaaatcat taacgatact
     1381 atccgcttcc agggcagtga acccccaaag gacaaaacta tcattctgga gcaactccgt
     1441 aagatctcgt tgcctctgta ctccattttg agcgctctga caatcctcgg tatgattatg
     1501 gcttcagcct tcttgttctt caacatcaag aaccgtaacc agaagctgat taaaatgtct
     1561 tcaccctaca tgaacaactt gatcattctg ggtggtatgc tgtcttacgc ctcaatcttc
     1621 ctcttcggat tggacggtag tttcgtgtcg gagaagacct tcgaaactct ctgcacagtc
     1681 aggacgtgga tcttgacagt tggctacaca acggcattcg gagcgatgtt cgctaagacc
     1741 tggagagtgc acgctatctt caagaacgtc aagatgaaaa agaaaatcat taaggaccag
     1801 aaactgctcg tcatcgttgg cggaatgttg ctgattgacc tgtgcatcct catttgttgg
     1861 caagcagttg atccactgcg ccgtaccgtg gaaaagtact ccatggagcc ggaccctgcg
     1921 ggaagggata tcagcattag acctctcttg gagcactgcg aaaacaccca tatgactatc
     1981 tggctgggta ttgtctacgc ctacaagggt ctgctcatgc tcttcggctg tttcttggca
     2041 tgggagacac gcaacgtgtc tatccccgcg ctgaacgaca gtaaatacat tggcatgtcg
     2101 gtttacaacg tgggaatcat gtgcatcatt ggtgcagcgg tttcattctt gactagggat
     2161 cagccaaacg tgcaattctg tattgtcgct ctggttatca ttttctgcag tacaatcacg
     2221 ctctgtttgg tgttcgtccc gaagttgatc acactgagaa cgaaccctga cgctgccacc
     2281 cagaacagga gattccaatt cactcagaac caaaagaaag aggattccaa gacctccact
     2341 agcgtgacaa gcgtcaacca ggcctctacg tcaaggctcg aaggtttgca aagcgagaac
     2401 cacaggctga gaatgaagat caccgaattg gacaaagatc tggaggaagt caccatgcag
     2461 ctccaagaca cttaa
//
