LOCUS       sequence-304708-        3829 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3829)
  AUTHORS   Prior JE, Lynch MD, Gill RT
  TITLE     Broad-host-range vectors for protein expression across gram negative
            hosts.
  JOURNAL   Biotechnol Bioeng. 2010 Jun 1. 106(2):326-32.
  PUBMED    20148414
REFERENCE   2  (bases 1 to 3829)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biotechnol 
            Bioeng. 2010 Jun 1. 106(2):326-32."
COMMENT     Sequence Label: pBTBX-2
FEATURES             Location/Qualifiers
     source          1..3829
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(60..96)
                     /label=soxR terminator
                     /note="bidirectional E. coli soxR transcription terminator"
     rep_origin      102..873
                     /label=pBBR1 oriV
                     /note="replication origin of the broad-host-range plasmid 
                     pBBR1 from Bordetella bronchiseptica; requires the pBBR1 
                     Rep protein for replication"
     CDS             874..1539
                     /codon_start=1
                     /product="replication protein for the broad-host-range 
                     plasmid pBBR1 from Bordetella bronchiseptica"
                     /label=pBBR1 Rep
                     /translation="MATQSREIGIQAKNKPGHWVQTERKAHEAWAGLIARKPTAAMLLH
                     HLVAQMGHQNAVVVSQKTLSKLIGRSLRTVQYAVKDLVAERWISVVKLNGPGTVSAYVV
                     NDRVAWGQPRDQLRLSVFSAAVVVDHDDQDESLLGHGDLRRIPTLYPGEQQLPTGPGEE
                     PPSQPGIPGMEPDLPALTETEEWERRGQQRLPMPDEPCFLDDGEPLEPPTRVTLPRRT"
     CDS             1629..2444
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNGDRVFRLAQAQSRMNNGLVGA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(1702..1721)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     terminator      complement(2461..2492)
                     /label=tonB terminator
                     /note="bidirectional E. coli tonB-P14 transcription 
                     terminator"
     CDS             complement(2520..3398)
                     /codon_start=1
                     /gene="araC"
                     /product="L-arabinose regulatory protein"
                     /label=araC
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKGY
                     ILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRAYW
                     HEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLLLRR
                     MEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQLGIS
                     VLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCE
                     EKVNDVAVKLS"
     promoter        3425..3709
                     /gene="araBAD"
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the 
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
ORIGIN
        1 aacgaattca agcttgatat cattcaggac gagcctcaga ctccagcgta actggactga
       61 aaacaaacta aagcgccctt gtggcgcttt agttttgttc cgcggccacc ggctggctcg
      121 cttcgctcgg cccgtggaca accctgctgg acaagctgat ggacaggctg cgcctgccca
      181 cgagcttgac cacagggatt gcccaccggc tacccagcct tcgaccacat acccaccggc
      241 tccaactgcg cggcctgcgg ccttgcccca tcaatttttt taattttctc tggggaaaag
      301 cctccggcct gcggcctgcg cgcttcgctt gccggttgga caccaagtgg aaggcgggtc
      361 aaggctcgcg cagcgaccgc gcagcggctt ggccttgacg cgcctggaac gacccaagcc
      421 tatgcgagtg ggggcagtcg aagggcgaag cccgcccgcc tgccccccga gcctcacggc
      481 ggcgagtgcg ggggttccaa gggggcagcg ccaccttggg caaggccgaa ggccgcgcag
      541 tcgatcaaca agccccggag gggccacttt ttgccggagg gggagccgcg ccgaaggcgt
      601 gggggaaccc cgcaggggtg cccttctttg ggcaccaaag aactagatat agggcgaaat
      661 gcgaaagact taaaaatcaa caacttaaaa aaggggggta cgcaacagct cattgcggca
      721 ccccccgcaa tagctcattg cgtaggttaa agaaaatctg taattgactg ccacttttac
      781 gcaacgcata attgttgtcg cgctgccgaa aagttgcagc tgattgcgca tggtgccgca
      841 accgtgcggc acccctaccg catggagata agcatggcca cgcagtccag agaaatcggc
      901 attcaagcca agaacaagcc cggtcactgg gtgcaaacgg aacgcaaagc gcatgaggcg
      961 tgggccgggc ttattgcgag gaaacccacg gcggcaatgc tgctgcatca cctcgtggcg
     1021 cagatgggcc accagaacgc cgtggtggtc agccagaaga cactttccaa gctcatcgga
     1081 cgttctttgc ggacggtcca atacgcagtc aaggacttgg tggccgagcg ctggatctcc
     1141 gtcgtgaagc tcaacggccc cggcaccgtg tcggcctacg tggtcaatga ccgcgtggcg
     1201 tggggccagc cccgcgacca gttgcgcctg tcggtgttca gtgccgccgt ggtggttgat
     1261 cacgacgacc aggacgaatc gctgttgggg catggcgacc tgcgccgcat cccgaccctg
     1321 tatccgggcg agcagcaact accgaccggc cccggcgagg agccgcccag ccagcccggc
     1381 attccgggca tggaaccaga cctgccagcc ttgaccgaaa cggaggaatg ggaacgacgc
     1441 gggcagcagc gcctgccgat gcccgatgag ccgtgttttc tggacgatgg cgagccgttg
     1501 gagccgccga cacgggtcac gctgccgcgc cgtacgtaag aggttccaac tttcaccata
     1561 atgaaataag atcactaccg ggcgtatttt ttgagttatc gagattttca ggagctaagg
     1621 aagctaaaat gagccatatt caacgggaaa cgtcttgctc gaggccgcga ttaaattcca
     1681 acatggatgc tgatttatat gggtataaat gggctcgcga taatgtcggg caatcaggtg
     1741 cgacaatcta tcgattgtat gggaagcccg atgcgccaga gttgtttctg aaacatggca
     1801 aaggtagcgt tgccaatgat gttacagatg agatggtcag gctaaactgg ctgacggaat
     1861 ttatgcctct tccgaccatc aagcatttta tccgtactcc tgatgatgca tggttactca
     1921 ccactgcgat cccagggaaa acagcattcc aggtattaga agaatatcct gattcaggtg
     1981 aaaatattgt tgatgcgctg gcagtgttcc tgcgccggtt gcattcgatt cctgtttgta
     2041 attgtccttt taacggcgat cgcgtatttc gtctcgctca ggcgcaatca cgaatgaata
     2101 acggtttggt tggtgcgagt gattttgatg acgagcgtaa tggctggcct gttgaacaag
     2161 tctggaaaga aatgcataag cttttgccat tctcaccgga ttcagtcgtc actcatggtg
     2221 atttctcact tgataacctt atttttgacg aggggaaatt aataggttgt attgatgttg
     2281 gacgagtcgg aatcgcagac cgataccagg atcttgccat cctatggaac tgcctcggtg
     2341 agttttctcc ttcattacag aaacggcttt ttcaaaaata tggtattgat aatcctgata
     2401 tgaataaatt gcagtttcac ttgatgctcg atgagttttt ctgagggcgg atccccctca
     2461 agtcaaaagc ctccggtcgg aggtttttga ctttctgcta tggaggtcag gtatgatttt
     2521 tatgacaact tgacggctac atcattcact ttttcttcac aaccggcacg gaactcgctc
     2581 gggctggccc cggtgcattt tttaaatacc cgcgagaaat agagttgatc gtcaaaacca
     2641 acattgcgac cgacggtggc gataggcatc cgggtggtgc tcaaaagcag cttcgcctgg
     2701 ctgatacgtt ggtcctcgcg ccagcttaag acgctaatcc ctaactgctg gcggaaaaga
     2761 tgtgacagac gcgacggcga caagcaaaca tgctgtgcga cgctggcgat atcaaaattg
     2821 ctgtctgcca ggtgatcgct gatgtactga caagcctcgc gtacccgatt atccatcggt
     2881 ggatggagcg actcgttaat cgcttccatg cgccgcagta acaattgctc aagcagattt
     2941 atcgccagca gctccgaata gcgcccttcc ccttgcccgg cgttaatgat ttgcccaaac
     3001 aggtcgctga aatgcggctg gtgcgcttca tccgggcgaa agaaccccgt attggcaaat
     3061 attgacggcc agttaagcca ttcatgccag taggcgcgcg gacgaaagta aacccactgg
     3121 tgataccatt cgcgagcctc cggatgacga ccgtagtgat gaatctctcc tggcgggaac
     3181 agcaaaatat cacccggtcg gcaaacaaat tctcgtccct gatttttcac caccccctga
     3241 ccgcgaatgg tgagattgag aatataacct ttcattccca gcggtcggtc gataaaaaaa
     3301 tcgagataac cgttggcctc aatcggcgtt aaacccgcca ccagatgggc attaaacgag
     3361 tatcccggca gcaggggatc attttgcgct tcagccatac ttttcatact cccgccattc
     3421 agagaagaaa ccaattgtcc atattgcatc agacattgcc gtcactgcgt cttttactgg
     3481 ctcttctcgc taaccaaacc ggtaaccccg cttattaaaa gcattctgta acaaagcggg
     3541 accaaagcca tgacaaaaac gcgtaacaaa agtgtctata atcacggcag aaaagtccac
     3601 attgattatt tgcacggcgt cacactttgc tatgccataa catttttatc cataagatta
     3661 gcggatccta cctgacgctt tttatcgcaa ctctctactg tttctccata cccgtttttt
     3721 tgggctagca ccgcctatct cgtgtgagat aggcggagat acgaacttta agaaggagat
     3781 atacccatgg tcaaatggtc agtattgagc gatatctaga gaattcgtc
//
