LOCUS       sequence-304722-        9997 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses a sgRNA targeting mouse Htt promoter for gene activation 
            using the SAM system.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9997)
  AUTHORS   Shen M, Wang F, Li M, Sah N, Stockton ME, Tidei JJ, Gao Y, 
            Korabelnikov T, Kannan S, Vevea JD, Chapman ER, Bhattacharyya A, van
            Praag H, Zhao X
  TITLE     Reduced mitochondrial fusion and Huntingtin levels contribute to 
            impaired dendritic maturation and behavioral deficits in Fmr1-mutant
            mice.
  JOURNAL   Nat Neurosci. 2019 Mar;22(3):386-400. doi: 
            10.1038/s41593-019-0338-y. Epub 2019 Feb 11.
  PUBMED    30742117
REFERENCE   2  (bases 1 to 9997)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41593-019-0338-y"; journalName: "Nat Neurosci"; date: 
            "2019-03"; volume: "22"; issue: "3"; pages: "386-400"
COMMENT     Sequence Label: 61427-sgHtt_10
FEATURES             Location/Qualifiers
     source          1..9997
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        29..277
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     29..49
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     200..219
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        316..334
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     misc_RNA        386..404
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     promoter        528..1705
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          759..1696
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1653..1673
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             1712..2086
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     misc_feature    2111..2699
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2164..2184)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2582..2593)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(2702..2718)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2703..2719)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             3224..3404
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(3430..3447)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    3436..3660
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      3706..4134
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3793..3812)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4003..4024
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4143..4163)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        4148..4477
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4328..4463
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     4390..4409
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        4525..4572
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             4591..4965
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    5095..5216
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5132..5151)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5186..5205
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(5265..5281)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5265..5281)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5278..5300)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5289..5305
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5313..5319,5320..5337,5338..5343))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5358..5379
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5496..5513)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5667..6255)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5747..5766)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6426..7217,7218..7286))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7049..7068
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7287..7391)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(7466..7485)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        7657..8036
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        8038..8236
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     8191..8211
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             8254..8434
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    8481..8606
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    9099..9332
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             9517..9561
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    9859..9976
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 agagatccag tttggttaat tagctagcga gggcctattt cccatgattc cttcatattt
       61 gcatatacga tacaaggctg ttagagagat aattggaatt aatttgactg taaacacaaa
      121 gatattagta caaaatacgt gacgtagaaa gtaataattt cttgggtagt ttgcagtttt
      181 aaaattatgt tttaaaatgg actatcatat gcttaccgta acttgaaagt atttcgattt
      241 cttggcttta tatatcttgt ggaaaggacg aaacaccggg tgtagggtgg ctttacgcgt
      301 tttagagcta ggccaacatg aggatcaccc atgtctgcag ggcctagcaa gttaaaataa
      361 ggctagtccg ttatcaactt ggccaacatg aggatcaccc atgtctgcag ggccaagtgg
      421 caccgagtcg gtgctttttt tggatcctgc aaagatggat aaagttttaa acagagagga
      481 atctttgcag ctaatggacc ttctaggtct tgaaaggagt gggaattggc tccggtgccc
      541 gtcagtgggc agagcgcaca tcgcccacag tccccgagaa gttgggggga ggggtcggca
      601 attgatccgg tgcctagaga aggtggcgcg gggtaaactg ggaaagtgat gtcgtgtact
      661 ggctccgcct ttttcccgag ggtgggggag aaccgtatat aagtgcagta gtcgccgtga
      721 acgttctttt tcgcaacggg tttgccgcca gaacacaggt aagtgccgtg tgtggttccc
      781 gcgggcctgg cctctttacg ggttatggcc cttgcgtgcc ttgaattact tccactggct
      841 gcagtacgtg attcttgatc ccgagcttcg ggttggaagt gggtgggaga gttcgaggcc
      901 ttgcgcttaa ggagcccctt cgcctcgtgc ttgagttgag gcctggcctg ggcgctgggg
      961 ccgccgcgtg cgaatctggt ggcaccttcg cgcctgtctc gctgctttcg ataagtctct
     1021 agccatttaa aatttttgat gacctgctgc gacgcttttt ttctggcaag atagtcttgt
     1081 aaatgcgggc caagatctgc acactggtat ttcggttttt ggggccgcgg gcggcgacgg
     1141 ggcccgtgcg tcccagcgca catgttcggc gaggcggggc ctgcgagcgc ggccaccgag
     1201 aatcggacgg gggtagtctc aagctggccg gcctgctctg gtgcctggcc tcgcgccgcc
     1261 gtgtatcgcc ccgccctggg cggcaaggct ggcccggtcg gcaccagttg cgtgagcgga
     1321 aagatggccg cttcccggcc ctgctgcagg gagctcaaaa tggaggacgc ggcgctcggg
     1381 agagcgggcg ggtgagtcac ccacacaaag gaaaagggcc tttccgtcct cagccgtcgc
     1441 ttcatgtgac tccacggagt accgggcgcc gtccaggcac ctcgattagt tctcgagctt
     1501 ttggagtacg tcgtctttag gttgggggga ggggttttat gcgatggagt ttccccacac
     1561 tgagtgggtg gagactgaag ttaggccagc ttggcacttg atgtaattct ccttggaatt
     1621 tgcccttttt gagtttggat cttggttcat tctcaagcct cagacagtgg ttcaaagttt
     1681 ttttcttcca tttcaggtgt cgtgatgtac aatggccaag ttgaccagtg ccgttccggt
     1741 gctcaccgcg cgcgacgtcg ccggagcggt cgagttctgg accgaccggc tcgggttctc
     1801 ccgggacttc gtggaggacg acttcgccgg tgtggtccgg gacgacgtga ccctgttcat
     1861 cagcgcggtc caggaccagg tggtgccgga caacaccctg gcctgggtgt gggtgcgcgg
     1921 cctggacgag ctgtacgccg agtggtcgga ggtcgtgtcc acgaacttcc gggacgcctc
     1981 cgggccggcc atgaccgaga tcggcgagca gccgtggggg cgggagttcg ccctgcgcga
     2041 cccggccggc aactgcgtgc acttcgtggc cgaggagcag gactgagaat tcgatatcaa
     2101 gcttatcggt aatcaacctc tggattacaa aatttgtgaa agattgactg gtattcttaa
     2161 ctatgttgct ccttttacgc tatgtggata cgctgcttta atgcctttgt atcatgctat
     2221 tgcttcccgt atggctttca ttttctcctc cttgtataaa tcctggttgc tgtctcttta
     2281 tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc
     2341 aacccccact ggttggggca ttgccaccac ctgtcagctc ctttccggga ctttcgcttt
     2401 ccccctccct attgccacgg cggaactcat cgccgcctgc cttgcccgct gctggacagg
     2461 ggctcggctg ttgggcactg acaattccgt ggtgttgtcg gggaaatcat cgtcctttcc
     2521 ttggctgctc gcctgtgttg ccacctggat tctgcgcggg acgtccttct gctacgtccc
     2581 ttcggccctc aatccagcgg accttccttc ccgcggcctg ctgccggctc tgcggcctct
     2641 tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc ctttgggccg cctccccgca
     2701 tcgataccgt cgacctcgag acctagaaaa acatggagca atcacaagta gcaatacagc
     2761 agctaccaat gctgattgtg cctggctaga agcacaagag gaggaggagg tgggttttcc
     2821 agtcacacct caggtacctt taagaccaat gacttacaag gcagctgtag atcttagcca
     2881 ctttttaaaa gaaaaggggg gactggaagg gctaattcac tcccaacgaa gacaagatat
     2941 ccttgatctg tggatctacc acacacaagg ctacttccct gattggcaga actacacacc
     3001 agggccaggg atcagatatc cactgacctt tggatggtgc tacaagctag taccagttga
     3061 gcaagagaag gtagaagaag ccaatgaagg agagaacacc cgcttgttac accctgtgag
     3121 cctgcatggg atggatgacc cggagagaga agtattagag tggaggtttg acagccgcct
     3181 agcatttcat cacatggccc gagagctgca tccggactgt actgggtctc tctggttaga
     3241 ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata
     3301 aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta
     3361 gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagggccc gtttaaaccc
     3421 gctgatcagc ctcgactgtg ccttctagtt gccagccatc tgttgtttgc ccctcccccg
     3481 tgccttcctt gaccctggaa ggtgccactc ccactgtcct ttcctaataa aatgaggaaa
     3541 ttgcatcgca ttgtctgagt aggtgtcatt ctattctggg gggtggggtg gggcaggaca
     3601 gcaaggggga ggattgggaa gacaatagca ggcatgctgg ggatgcggtg ggctctatgg
     3661 cttctgaggc ggaaagaacc agctggggct ctagggggta tccccacgcg ccctgtagcg
     3721 gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg
     3781 ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc
     3841 cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct ttacggcacc
     3901 tcgaccccaa aaaacttgat tagggtgatg gttcacgtag tgggccatcg ccctgataga
     3961 cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc ttgttccaaa
     4021 ctggaacaac actcaaccct atctcggtct attcttttga tttataaggg attttgccga
     4081 tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattaattct
     4141 gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc tccccagcag gcagaagtat
     4201 gcaaagcatg catctcaatt agtcagcaac caggtgtgga aagtccccag gctccccagc
     4261 aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accatagtcc cgcccctaac
     4321 tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact
     4381 aatttttttt atttatgcag aggccgaggc cgcctctgcc tctgagctat tccagaagta
     4441 gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag ctcccgggag cttgtatatc
     4501 cattttcgga tctgatcagc acgtgttgac aattaatcat cggcatagta tatcggcata
     4561 gtataatacg acaaggtgag gaactaaacc atggccaagt tgaccagtgc cgttccggtg
     4621 ctcaccgcgc gcgacgtcgc cggagcggtc gagttctgga ccgaccggct cgggttctcc
     4681 cgggacttcg tggaggacga cttcgccggt gtggtccggg acgacgtgac cctgttcatc
     4741 agcgcggtcc aggaccaggt ggtgccggac aacaccctgg cctgggtgtg ggtgcgcggc
     4801 ctggacgagc tgtacgccga gtggtcggag gtcgtgtcca cgaacttccg ggacgcctcc
     4861 gggccggcca tgaccgagat cggcgagcag ccgtgggggc gggagttcgc cctgcgcgac
     4921 ccggccggca actgcgtgca cttcgtggcc gaggagcagg actgacacgt gctacgagat
     4981 ttcgattcca ccgccgcctt ctatgaaagg ttgggcttcg gaatcgtttt ccgggacgcc
     5041 ggctggatga tcctccagcg cggggatctc atgctggagt tcttcgccca ccccaacttg
     5101 tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa
     5161 gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat
     5221 gtctgtatac cgtcgacctc tagctagagc ttggcgtaat catggtcata gctgtttcct
     5281 gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt
     5341 aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc
     5401 gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg
     5461 agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg
     5521 gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca
     5581 gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac
     5641 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac
     5701 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg
     5761 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac
     5821 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat
     5881 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag
     5941 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac
     6001 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt
     6061 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt
     6121 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc
     6181 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga
     6241 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac
     6301 gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc
     6361 cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct
     6421 gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca
     6481 tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct
     6541 ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca
     6601 ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc
     6661 atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg
     6721 cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct
     6781 tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa
     6841 aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta
     6901 tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc
     6961 ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg
     7021 agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa
     7081 gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg
     7141 agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc
     7201 accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg
     7261 gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat
     7321 cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata
     7381 ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tcgacggatc gggagatctc
     7441 ccgatcccct atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt
     7501 atctgctccc tgcttgtgtg ttggaggtcg ctgagtagtg cgcgagcaaa atttaagcta
     7561 caacaaggca aggcttgacc gacaattgca tgaagaatct gcttagggtt aggcgttttg
     7621 cgctgcttcg cgatgtacgg gccagatata cgcgttgaca ttgattattg actagttatt
     7681 aatagtaatc aattacgggg tcattagttc atagcccata tatggagttc cgcgttacat
     7741 aacttacggt aaatggcccg cctggctgac cgcccaacga cccccgccca ttgacgtcaa
     7801 taatgacgta tgttcccata gtaacgccaa tagggacttt ccattgacgt caatgggtgg
     7861 agtatttacg gtaaactgcc cacttggcag tacatcaagt gtatcatatg ccaagtacgc
     7921 cccctattga cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag tacatgacct
     7981 tatgggactt tcctacttgg cagtacatct acgtattagt catcgctatt accatggtga
     8041 tgcggttttg gcagtacatc aatgggcgtg gatagcggtt tgactcacgg ggatttccaa
     8101 gtctccaccc cattgacgtc aatgggagtt tgttttggca ccaaaatcaa cgggactttc
     8161 caaaatgtcg taacaactcc gccccattga cgcaaatggg cggtaggcgt gtacggtggg
     8221 aggtctatat aagcagcgcg ttttgcctgt actgggtctc tctggttaga ccagatctga
     8281 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
     8341 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
     8401 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
     8461 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
     8521 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
     8581 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
     8641 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
     8701 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
     8761 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     8821 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     8881 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     8941 aagcggccgc tgatcttcag acctggagga ggagatatga gggacaattg gagaagtgaa
     9001 ttatataaat ataaagtagt aaaaattgaa ccattaggag tagcacccac caaggcaaag
     9061 agaagagtgg tgcagagaga aaaaagagca gtgggaatag gagctttgtt ccttgggttc
     9121 ttgggagcag caggaagcac tatgggcgca gcgtcaatga cgctgacggt acaggccaga
     9181 caattattgt ctggtatagt gcagcagcag aacaatttgc tgagggctat tgaggcgcaa
     9241 cagcatctgt tgcaactcac agtctggggc atcaagcagc tccaggcaag aatcctggct
     9301 gtggaaagat acctaaagga tcaacagctc ctggggattt ggggttgctc tggaaaactc
     9361 atttgcacca ctgctgtgcc ttggaatgct agttggagta ataaatctct ggaacagatt
     9421 tggaatcaca cgacctggat ggagtgggac agagaaatta acaattacac aagcttaata
     9481 cactccttaa ttgaagaatc gcaaaaccag caagaaaaga atgaacaaga attattggaa
     9541 ttagataaat gggcaagttt gtggaattgg tttaacataa caaattggct gtggtatata
     9601 aaattattca taatgatagt aggaggcttg gtaggtttaa gaatagtttt tgctgtactt
     9661 tctatagtga atagagttag gcagggatat tcaccattat cgtttcagac ccacctccca
     9721 accccgaggg gacccgacag gcccgaagga atagaagaag aaggtggaga gagagacaga
     9781 gacagatcca ttcgattagt gaacggatcg gcactgcgtg cgccaattct gcagacaaat
     9841 ggcagtattc atccacaatt ttaaaagaaa aggggggatt ggggggtaca gtgcagggga
     9901 aagaatagta gacataatag caacagacat acaaactaaa gaattacaaa aacaaattac
     9961 aaaaattcaa aattttcggg tttattacag ggacagc
//
