LOCUS       sequence-304730-        5282 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Plasmid harboring four plant enhancers (35S, AB80, Cab-1, and 
            rbcS-E9)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5282)
  AUTHORS   Jores T, Tonnies J, Dorrity MW, Cuperus J, Fields S, Queitsch C
  TITLE     Identification of Plant Enhancers and Their Constituent Elements by 
            STARR-seq in Tobacco Leaves.
  JOURNAL   Plant Cell. 2020 May 14. pii: tpc.20.00155. doi: 
            10.1105/tpc.20.00155.
  PUBMED    32409318
REFERENCE   2  (bases 1 to 5282)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant Cell.
            2020 May 14. pii: tpc.20.00155. doi: 10.1105/tpc.20.00155."
COMMENT     Sequence Label: pZS*11_4enh
FEATURES             Location/Qualifiers
     source          1..5282
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(443..537)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(join(726..1517,1518..1586))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1349..1368
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1587..1691)
                     /gene="bla"
                     /label=AmpR promoter
     promoter        join(2035..2053,2054..2059,2060..2076,2077..2082,
                     2083..2108)
                     /label=PLtetO-1 promoter
                     /note="modified phage lambda PL promoter with tet operator 
                     sites (Lutz and Bujard, 1997)"
     protein_bind    2035..2053
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2060..2078
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     RBS             2121..2143
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     terminator      3149..3235
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     CDS             complement(3773..4723)
                     /codon_start=1
                     /gene="rep101"
                     /product="RepA protein needed for replication with the 
                     pSC101 origin"
                     /label=Rep101
                     /translation="MSELVVFKANELAISRYDLTEHETKLILCCVALLNPTIENPTRKE
                     RTVSFTYNQYAQMMNISRENAYGVLAKATRELMTRTVEIRNPLVKGFEIFQWTNYAKFS
                     SEKLELVFSEEILPYLFQLKKFIKYNLEHVKSFENKYSMRIYEWLLKELTQKKTHKANI
                     EISLDEFKFMLMLENNYHEFKRLNQWVLKPISKDLNTYSNMKLVVDKRGRPTDTLIFQV
                     ELDRQMDLVTELENNQIKMNGDKIPTTITSDSYLHNGLRKTLHDALTAKIQLTSFEAKF
                     LSDMQSKYDLNGSFSWLTQKQRTTLENILAKYGRI"
     rep_origin      4771..4993
                     /label=pSC101 ori
                     /note="low-copy replication origin that requires the Rep101
                     protein"
ORIGIN
        1 catgtttaat ccttatactg ttgttagttt tttcagttag cttagtgggc atcttacacg
       61 tggcattatt atcctattgg tggctaatga taaggttagc acacaaaact tttcaatctt
      121 gtgtggttaa tatgactgca aagtttatca ttttcacaat ccaacaaact ggttctaggc
      181 agtggggcgc cttttatgga ttcatgcaag gaaactaccc ataatacaag aaaagcccgt
      241 cacgggcttc tcagggcgtt ttatggcggg tctgctatgt ggtgctatct gactttttgc
      301 tgttcagcag ttcctgccct ctgattttcc agtctgacca cttcggatta tcccgtgaca
      361 ggtcattcag actggctaat gcacccagta aggcagcggt atcatcaaca ggcttacccg
      421 tcttactgtc cctagtgctt ggattctcac caataaaaaa cgcccggcgg caaccgagcg
      481 ttctgaacaa atccagatgg agttctgagg tcattactgg atctatcaac aggagtccaa
      541 gcgagctcag atctctctgc cgacagtggt cccaaagatg gacccccacc cacgaggagc
      601 atcgtggaaa aagaagacgt tccaaccacg tcttcaaagc aagtggattg atgtgacatc
      661 tccactgacg taagggatga cgcacaatcc cactatcctt cgagctcgta aacttggtct
      721 gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca
      781 tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct
      841 ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca
      901 ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc
      961 atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg
     1021 cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct
     1081 tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa
     1141 aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta
     1201 tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc
     1261 ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg
     1321 agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa
     1381 gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg
     1441 agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc
     1501 accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg
     1561 gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat
     1621 cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata
     1681 ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tccaacatat ggcaccggag
     1741 gctttcgtct tcacctcgag atcgaatttg ttgggcaagc gcccagtgtg atgtacgttg
     1801 ggaaaacttg caagaggatg cgaccaaatg aactggtaaa ccatcccgtg agcgtggcct
     1861 acacatttta agccagcgga ctctttcgac ttgtcttaca aaagctggtc cagtcacgag
     1921 ccttagccct aaccatagcc acaagtacat ctcatccatt taaggcctct gcgtgcacca
     1981 atggcatcca agctgcagat ttcttttcac caccgtctct cttgtcagct cgagtcccta
     2041 tcagtgatag agattgacat ccctatcagt gatagagata ctgagcacat cagcaggacg
     2101 cactgaccga attcaataat tttgtttaac tttaagaagg agatggtacc atgagcaaag
     2161 gtgaagaact gttcaccggc gttgtgccaa ttctggttga gctggatggt gacgtgaatg
     2221 gccacaaatt ttccgtgtct ggtgaaggcg agggtgatgc tacttatggc aaactgactc
     2281 tgaaactgat ctgtaccacc ggcaaactgc ctgttccatg gccaactctg gtcactactc
     2341 tgggttacgg cctgatgtgt tttgcgcgtt acccggatca catgaaacag catgacttct
     2401 tcaaatctgc catgccggaa ggctatgtcc aagaacgtac gatctttttc aaggacgacg
     2461 gcaactataa aacccgtgcc gaagttaaat tcgagggtga caccctggtt aaccgcatcg
     2521 aactgaaagg cattgacttc aaagaggacg gcaacattct gggtcacaag ctggaataca
     2581 actacaactc ccacaacgtt tacattactg ctgacaagca gaaaaacggc atcaaagcaa
     2641 acttcaagat ccgtcacaac attgaagatg gtggcgtaca gctggcagat cactaccagc
     2701 agaacactcc aatcggtgat ggcccagtac tgctgccaga taaccattac ctgtcctacc
     2761 agagcaaact gtctaaagac ccgaacgaaa aacgtgacca catggtactg ctggaatttg
     2821 ttaccgcggc aggcattacc cacggtatgg acgaactgta taaatagaag cttaattagc
     2881 tgagtctaga ctgccataat gtcacaattt ttctcaaatc ttgtggctct caaacactgt
     2941 ataaaacacg acaaatgtgg acccaaaata tataccttac acttctgagt tagagaagca
     3001 gagccccata attaagccta ttttatgaaa aaaataatat tatgttgagt catatatcca
     3061 taagaatccc cacagtcaca catggaagag cagcattgga tacaaatgat atgaagattt
     3121 tgatccatga acggatttct agaggcatca aataaaacga aaggctcagt cgaaagactg
     3181 ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc ctgagtagga caaatccgcc
     3241 gccctagacc tagggtacgg gttttgctgc ccgcaaacgg gctgttctgg tgttgctagt
     3301 ttgttatcag aatcgcagat ccggcttcag ccggtttgcc ggctgaaagc gctatttctt
     3361 ccagaattgc catgattttt tccccacggg aggcgtcact ggctcccgtg ttgtcggcag
     3421 ctttgattcg ataagcagca tcgcctgttt caggctgtct atgtgtgact gttgagctgt
     3481 aacaagttgt ctcaggtgtt caatttcatg ttctagttgc tttgttttac tggtttcacc
     3541 tgttctatta ggtgttacat gctgttcatc tgttacattg tcgatctgtt catggtgaac
     3601 agctttgaat gcaccaaaaa ctcgtaaaag ctctgatgta tctatctttt ttacaccgtt
     3661 ttcatctgtg catatggaca gttttccctt tgatatgtaa cggtgaacag ttgttctact
     3721 tttgtttgtt agtcttgatg cttcactgat agatacaaga gccataagaa cctcagatcc
     3781 ttccgtattt agccagtatg ttctctagtg tggttcgttg tttttgcgtg agccatgaga
     3841 acgaaccatt gagatcatac ttactttgca tgtcactcaa aaattttgcc tcaaaactgg
     3901 tgagctgaat ttttgcagtt aaagcatcgt gtagtgtttt tcttagtccg ttatgtaggt
     3961 aggaatctga tgtaatggtt gttggtattt tgtcaccatt catttttatc tggttgttct
     4021 caagttcggt tacgagatcc atttgtctat ctagttcaac ttggaaaatc aacgtatcag
     4081 tcgggcggcc tcgcttatca accaccaatt tcatattgct gtaagtgttt aaatctttac
     4141 ttattggttt caaaacccat tggttaagcc ttttaaactc atggtagtta ttttcaagca
     4201 ttaacatgaa cttaaattca tcaaggctaa tctctatatt tgccttgtga gttttctttt
     4261 gtgttagttc ttttaataac cactcataaa tcctcataga gtatttgttt tcaaaagact
     4321 taacatgttc cagattatat tttatgaatt tttttaactg gaaaagataa ggcaatatct
     4381 cttcactaaa aactaattct aatttttcgc ttgagaactt ggcatagttt gtccactgga
     4441 aaatctcaaa gcctttaacc aaaggattcc tgatttccac agttctcgtc atcagctctc
     4501 tggttgcttt agctaataca ccataagcat tttccctact gatgttcatc atctgagcgt
     4561 attggttata agtgaacgat accgtccgtt ctttccttgt agggttttca atcgtggggt
     4621 tgagtagtgc cacacagcat aaaattagct tggtttcatg ctccgttaag tcatagcgac
     4681 taatcgctag ttcatttgct ttgaaaacaa ctaattcaga catacatctc aattggtcta
     4741 ggtgatttta atcactatac caattgagat gggctagtca atgataatta ctagtccttt
     4801 tcccgggaga tctgggtatc tgtaaattct gctagacctt tgctggaaaa cttgtaaatt
     4861 ctgctagacc ctctgtaaat tccgctagac ctttgtgtgt tttttttgtt tatattcaag
     4921 tggttataat ttatagaata aagaaagaat aaaaaaagat aaaaagaata gatcccagcc
     4981 ctgtgtataa ctcactactt tagtcagttc cgcagtatta caaaaggatg tcgcaaacgc
     5041 tgtttgctcc tctacaaaac agaccttaaa accctaaagg cttaagtagc accctcgcaa
     5101 gctcgggcaa atcgctgaat attccttttg tctccgacca tcaggcacct gagtcgctgt
     5161 ctttttcgtg acattcagtt cgctgcgctc acggctctgg cagtgaatgg gggtaaatgg
     5221 cactacaggc gccacactaa catcgaatgt acttatctca ttagtttaaa ttattgtttg
     5281 at
//
