LOCUS       sequence-304733-        4620 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway-compatible Entry vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4620)
  AUTHORS   Kim DK, Knapp JJ, Kuang D, Chawla A, Cassonnet P, Lee H, 
            Sheykhkarimli D, Samavarchi-Tehrani P, Abdouni H, Rayhan A, Li R, 
            Pogoutse O, Coyaud E, van der Werf S, Demeret C, Gingras AC, Taipale
            M, Raught B, Jacob Y, Roth FP
  TITLE     A Comprehensive, Flexible Collection of SARS-CoV-2 Coding Regions.
  JOURNAL   G3 (Bethesda). 2020 Aug 6. pii: g3.120.401554. doi: 
            10.1534/g3.120.401554.
  PUBMED    32763951
REFERENCE   2  (bases 1 to 4620)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "G3 
            (Bethesda). 2020 Aug 6. pii: g3.120.401554. doi: 
            10.1534/g3.120.401554."
COMMENT     Sequence Label: pDONR223 SARS-CoV2 TEV-NSP13
FEATURES             Location/Qualifiers
     source          1..4620
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(130..157)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(249..335)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     384..406
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     398..415
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     399..415
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    431..530
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             530..550
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     protein_bind    complement(2361..2460)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2477..2496)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2478..2496)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2501..2517)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2501..2517)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             2948..3739
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      3832..4420
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4321..4340
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4574..4591
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 gcctctcccc gcgcgttggc cgattcatta atgcagctgg cacgacaggt ttcccgactg
       61 gaaagcgggc agtgagcgca acgcaattaa tacgcgtacc gctagccagg aagagtttgt
      121 agaaacgcaa aaaggccatc cgtcaggatg gccttctgct tagtttgatg cctggcagtt
      181 tatggcgggc gtcctgcccg ccaccctccg ggccgttgct tcacaacgtt caaatccgct
      241 cccggcggat ttgtcctact caggagagcg ttcaccgaca aacaacagat aaaacgaaag
      301 gcccagtctt ccgactgagc ctttcgtttt atttgatgcc tggcagttcc ctactctcgc
      361 gttaacgcta gcatggatgt tttcccagtc acgacgttgt aaaacgacgg ccagtcttaa
      421 gctcgggccc caaataatga ttttattttg actgatagtg acctgttcgt tgcaacaaat
      481 tgatgagcaa tgctttttta taatgccaac tttgtacaaa aaagttggcg aaaacctgta
      541 cttccaaggc atggccgtgg gcgcttgcgt gctgtgcaac tctcagacca gcctgagatg
      601 tggagcttgc atccgcaggc ctttcctgtg ctgcaagtgc tgctacgacc acgtgatcag
      661 cacctcccac aagctggtgc tgtccgtgaa cccatacgtg tgcaacgctc ctggttgtga
      721 cgtgactgac gtgacccagc tgtacctggg cggaatgagc tactactgca agtcccacaa
      781 gccccctatc agcttcccac tgtgcgctaa cggccaggtg ttcggactgt acaagaacac
      841 ctgcgtgggc tccgacaacg tgaccgactt caacgccatc gctacctgcg actggaccaa
      901 cgccggagac tacatcctgg ctaacacctg caccgaaagg ctgaagctgt tcgccgctga
      961 gaccctgaag gccaccgagg aaaccttcaa gctgtcttac ggtatcgcta ccgtgcgtga
     1021 ggtgctgtct gaccgcgaac tgcacctgtc ttgggaagtg ggcaagccaa gaccacccct
     1081 gaaccgtaac tacgtgttca ccggatacag ggtgaccaag aacagcaagg tgcagatcgg
     1141 agagtacacc ttcgaaaagg gagactacgg tgacgccgtg gtgtacagag gaaccaccac
     1201 ctacaagctg aacgtgggtg actacttcgt gctgacctcc cacaccgtga tgccactgtc
     1261 tgctcccacc ctggtgcctc aggaacacta cgtgcgcatc accggactgt acccaaccct
     1321 gaacatctcc gacgagttca gctccaacgt ggccaactac cagaaagtgg gcatgcagaa
     1381 gtactccacc ctgcagggac ctccaggtac cggcaagtct cacttcgcca tcggtctggc
     1441 tctgtactac ccctctgccc gtatcgtgta cactgcttgt tctcacgctg ctgtggatgc
     1501 tctgtgcgag aaggctctga agtacctgcc tatcgacaag tgctcccgca tcatcccagc
     1561 cagagctcgt gtggaatgct tcgacaagtt caaggtgaac tctaccctgg agcagtacgt
     1621 gttctgcacc gtgaacgccc tgcctgaaac caccgctgac atcgtggtgt tcgacgagat
     1681 cagcatggcc accaactacg acctgtctgt ggtgaacgct agactgaggg ctaagcacta
     1741 cgtgtacatc ggtgacccag ctcagctgcc tgctcctcgt accctgctga ccaagggcac
     1801 cctggagcca gaatacttca acagcgtgtg ccgcctgatg aagaccatcg gtccagacat
     1861 gttcctgggt acctgccgcc gctgccctgc tgaaatcgtg gacaccgtgt ctgctctggt
     1921 gtacgacaac aagctgaagg cccacaagga caagagcgcc cagtgcttca agatgttcta
     1981 caagggagtg atcacccacg acgtgtctag cgccatcaac aggccccaga tcggtgtggt
     2041 gcgcgagttc ctgaccagga accccgcctg gagaaaggcc gtgttcatct ccccttacaa
     2101 ctctcagaac gccgtggctt ccaagatcct gggtctgcct acccagaccg tggactcctc
     2161 tcagggctct gaatacgact acgtgatctt cacccagacc accgagaccg cccacagctg
     2221 caacgtgaac aggttcaacg tggccatcac cagagctaaa gtgggaatcc tgtgcatcat
     2281 gagcgacaga gacctgtacg acaagctgca gttcacctcc ctggagatcc cccgcaggaa
     2341 cgtggctacc ctgcagtaat acccaacttt cttgtacaaa gttggcatta taagaaagca
     2401 ttgcttatca atttgttgca acgaacaggt cactatcagt caaaataaaa tcattatttg
     2461 ccatccagct gatatcccct atagtgagtc gtattacatg gtcatagctg tttcctggca
     2521 gctctggccc gtgtctcaaa atctctgatg ttacattgca caagataaaa atatatcatc
     2581 atgcctcctc tagaccagcc aggacagaaa tgcctcgact tcgctgctac ccaaggttgc
     2641 cgggtgacgc acaccgtgga aacggatgaa ggcacgaacc cagtggacat aagcctgttc
     2701 ggttcgtaag ctgtaatgca agtagcgtat gcgctcacgc aactggtcca gaaccttgac
     2761 cgaacgcagc ggtggtaacg gcgcagtggc ggttttcatg gcttgttatg actgtttttt
     2821 tggggtacag tctatgcctc gggcatccaa gcagcaagcg cgttacgccg tgggtcgatg
     2881 tttgatgtta tggagcagca acgatgttac gcagcagggc agtcgcccta aaacaaagtt
     2941 aaacattatg agggaagcgg tgatcgccga agtatcgact caactatcag aggtagttgg
     3001 cgtcatcgag cgccatctcg aaccgacgtt gctggccgta catttgtacg gctccgcagt
     3061 ggatggcggc ctgaagccac acagtgatat tgatttgctg gttacggtga ccgtaaggct
     3121 tgatgaaaca acgcggcgag ctttgatcaa cgaccttttg gaaacttcgg cttcccctgg
     3181 agagagcgag attctccgcg ctgtagaagt caccattgtt gtgcacgacg acatcattcc
     3241 gtggcgttat ccagctaagc gcgaactgca atttggagaa tggcagcgca atgacattct
     3301 tgcaggtatc ttcgagccag ccacgatcga cattgatctg gctatcttgc tgacaaaagc
     3361 aagagaacat agcgttgcct tggtaggtcc agcggcggag gaactctttg atccggttcc
     3421 tgaacaggat ctatttgagg cgctaaatga aaccttaacg ctatggaact cgccgcccga
     3481 ctgggctggc gatgagcgaa atgtagtgct tacgttgtcc cgcatttggt acagcgcagt
     3541 aaccggcaaa atcgcgccga aggatgtcgc tgccgactgg gcaatggagc gcctgccggc
     3601 ccagtatcag cccgtcatac ttgaagctag acaggcttat cttggacaag aagaagatcg
     3661 cttggcctcg cgcgcagatc agttggaaga atttgtccac tacgtgaaag gcgagatcac
     3721 caaggtagtc ggcaaataac cctcgagcca cccatgacca aaatccctta acgtgagtta
     3781 cgcgtcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc
     3841 tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt
     3901 ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc
     3961 gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc
     4021 tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg
     4081 cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg
     4141 gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga
     4201 actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc
     4261 ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg
     4321 gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg
     4381 atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt
     4441 tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc
     4501 tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg
     4561 aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc
//
