LOCUS       sequence-304735-        3855 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway-compatible Entry vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3855)
  AUTHORS   Kim DK, Knapp JJ, Kuang D, Chawla A, Cassonnet P, Lee H, 
            Sheykhkarimli D, Samavarchi-Tehrani P, Abdouni H, Rayhan A, Li R, 
            Pogoutse O, Coyaud E, van der Werf S, Demeret C, Gingras AC, Taipale
            M, Raught B, Jacob Y, Roth FP
  TITLE     A Comprehensive, Flexible Collection of SARS-CoV-2 Coding Regions.
  JOURNAL   G3 (Bethesda). 2020 Aug 6. pii: g3.120.401554. doi: 
            10.1534/g3.120.401554.
  PUBMED    32763951
REFERENCE   2  (bases 1 to 3855)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "G3 
            (Bethesda). 2020 Aug 6. pii: g3.120.401554. doi: 
            10.1534/g3.120.401554."
COMMENT     Sequence Label: pDONR223 SARS-CoV2 TEV-NSP15
FEATURES             Location/Qualifiers
     source          1..3855
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     114..133
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     367..384
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(543..570)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(662..748)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     797..819
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     811..828
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     812..828
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    844..943
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             943..963
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     protein_bind    complement(2009..2108)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2125..2144)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2126..2144)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2149..2165)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2149..2165)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             2596..3387
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      join(3480..3855,1..213)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg
       61 tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac
      121 gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg
      181 tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg
      241 ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct
      301 gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc
      361 gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa accgcctctc
      421 cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg
      481 ggcagtgagc gcaacgcaat taatacgcgt accgctagcc aggaagagtt tgtagaaacg
      541 caaaaaggcc atccgtcagg atggccttct gcttagtttg atgcctggca gtttatggcg
      601 ggcgtcctgc ccgccaccct ccgggccgtt gcttcacaac gttcaaatcc gctcccggcg
      661 gatttgtcct actcaggaga gcgttcaccg acaaacaaca gataaaacga aaggcccagt
      721 cttccgactg agcctttcgt tttatttgat gcctggcagt tccctactct cgcgttaacg
      781 ctagcatgga tgttttccca gtcacgacgt tgtaaaacga cggccagtct taagctcggg
      841 ccccaaataa tgattttatt ttgactgata gtgacctgtt cgttgcaaca aattgatgag
      901 caatgctttt ttataatgcc aactttgtac aaaaaagttg gcgaaaacct gtacttccaa
      961 ggcatgtctc tggagaacgt ggccttcaac gtggtgaaca agggtcactt cgacggtcag
     1021 cagggcgaag tgcctgtgag catcatcaac aacaccgtgt acaccaaggt ggacggcgtg
     1081 gacgtggagc tgttcgaaaa caagaccacc ctgccagtga acgtggcctt cgagctgtgg
     1141 gctaagcgca acatcaagcc cgtgcctgaa gtgaagatcc tgaacaacct gggagtggac
     1201 atcgccgcta acaccgtgat ctgggactac aagagggacg cccccgctca catcagcacc
     1261 atcggcgtgt gctccatgac cgacatcgcc aagaagccta ccgagaccat ctgcgctcca
     1321 ctgaccgtgt tcttcgacgg aagagtggac ggtcaggtgg acctgttccg caacgctagg
     1381 aacggcgtgc tgatcaccga gggctctgtg aagggactgc agccaagcgt gggacccaag
     1441 caggcctccc tgaacggtgt gaccctgatc ggcgaagctg tgaagaccca gttcaactac
     1501 tacaagaagg tggacggcgt ggtgcagcag ctgccagaga cctacttcac ccagtctaga
     1561 aacctgcagg aattcaagcc ccgtagccag atggagatcg acttcctgga actggccatg
     1621 gacgagttca tcgaaagata caagctggag ggatacgctt tcgaacacat cgtgtacggt
     1681 gacttctctc acagccagct gggcggactg cacctgctga tcggtctggc caagcgtttc
     1741 aaggagtccc ccttcgagct ggaagacttc atccctatgg actctaccgt gaagaactac
     1801 ttcatcaccg acgctcagac cggaagctcc aagtgcgtgt gcagcgtgat cgacctgctg
     1861 ctggacgact tcgtggagat catcaagtcc caggacctgt ccgtggtgtc taaggtggtg
     1921 aaggtgacca tcgactacac cgagatctcc ttcatgctgt ggtgcaagga cggccacgtg
     1981 gaaaccttct accctaagct gcagtaatac ccaactttct tgtacaaagt tggcattata
     2041 agaaagcatt gcttatcaat ttgttgcaac gaacaggtca ctatcagtca aaataaaatc
     2101 attatttgcc atccagctga tatcccctat agtgagtcgt attacatggt catagctgtt
     2161 tcctggcagc tctggcccgt gtctcaaaat ctctgatgtt acattgcaca agataaaaat
     2221 atatcatcat gcctcctcta gaccagccag gacagaaatg cctcgacttc gctgctaccc
     2281 aaggttgccg ggtgacgcac accgtggaaa cggatgaagg cacgaaccca gtggacataa
     2341 gcctgttcgg ttcgtaagct gtaatgcaag tagcgtatgc gctcacgcaa ctggtccaga
     2401 accttgaccg aacgcagcgg tggtaacggc gcagtggcgg ttttcatggc ttgttatgac
     2461 tgtttttttg gggtacagtc tatgcctcgg gcatccaagc agcaagcgcg ttacgccgtg
     2521 ggtcgatgtt tgatgttatg gagcagcaac gatgttacgc agcagggcag tcgccctaaa
     2581 acaaagttaa acattatgag ggaagcggtg atcgccgaag tatcgactca actatcagag
     2641 gtagttggcg tcatcgagcg ccatctcgaa ccgacgttgc tggccgtaca tttgtacggc
     2701 tccgcagtgg atggcggcct gaagccacac agtgatattg atttgctggt tacggtgacc
     2761 gtaaggcttg atgaaacaac gcggcgagct ttgatcaacg accttttgga aacttcggct
     2821 tcccctggag agagcgagat tctccgcgct gtagaagtca ccattgttgt gcacgacgac
     2881 atcattccgt ggcgttatcc agctaagcgc gaactgcaat ttggagaatg gcagcgcaat
     2941 gacattcttg caggtatctt cgagccagcc acgatcgaca ttgatctggc tatcttgctg
     3001 acaaaagcaa gagaacatag cgttgccttg gtaggtccag cggcggagga actctttgat
     3061 ccggttcctg aacaggatct atttgaggcg ctaaatgaaa ccttaacgct atggaactcg
     3121 ccgcccgact gggctggcga tgagcgaaat gtagtgctta cgttgtcccg catttggtac
     3181 agcgcagtaa ccggcaaaat cgcgccgaag gatgtcgctg ccgactgggc aatggagcgc
     3241 ctgccggccc agtatcagcc cgtcatactt gaagctagac aggcttatct tggacaagaa
     3301 gaagatcgct tggcctcgcg cgcagatcag ttggaagaat ttgtccacta cgtgaaaggc
     3361 gagatcacca aggtagtcgg caaataaccc tcgagccacc catgaccaaa atcccttaac
     3421 gtgagttacg cgtcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
     3481 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
     3541 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
     3601 agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc
     3661 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
     3721 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
     3781 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
     3841 tacaccgaac tgaga
//
