LOCUS       sequence-304744-        7404 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  tdTomato is expressed under the mycobacterial phsp60 heat shock 
            promoter for in vivo and in vitro detection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7404)
  AUTHORS   Kong Y, Yang D, Cirillo SL, Li S, Akin A, Francis KP, Maloney T, 
            Cirillo JD
  TITLE     Application of Fluorescent Protein Expressing Strains to Evaluation 
            of Anti-Tuberculosis Therapeutic Efficacy In Vitro and In Vivo.
  JOURNAL   PLoS One. 2016 Mar 2;11(3):e0149972. doi: 
            10.1371/journal.pone.0149972. eCollection 2016.
  PUBMED    26934495
REFERENCE   2  (bases 1 to 7404)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2016-03-2"; volume: "11(3):e0149972. doi"; pages: " 
            10.1371/journal.pone.0149972. eCollection 2016"
COMMENT     Sequence Label: pJDC122
FEATURES             Location/Qualifiers
     source          1..7404
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             583..612
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="ASMTGGQQMG"
     CDS             join(616..624,625..639)
                     /codon_start=1
                     /product="Xpress(TM) epitope tag, including an enterokinase
                     recognition and cleavage site"
                     /label=Xpress(TM) tag
                     /translation="DLYDDDDK"
     CDS             655..2085
                     /codon_start=1
                     /product="tandem dimeric (pseudo-monomeric) derivative of 
                     DsRed (Shaner et al., 2004)"
                     /label=tdTomato
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG
                     LVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIH
                     QALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRH
                     HLFLGHGTGSTGSGSSGTASSEDNNMAVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPY
                     EGTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVM
                     NFEDGGLVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGV
                     LKGEIHQALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYE
                     RSEGRHHLFLYGMDELYK"
     primer_bind     complement(2148..2166)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      2162..2209
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     rep_origin      2306..2761
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2393..2412)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2603..2624
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        2787..2891
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(3110..3129)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      complement(5325..5913)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5405..5424)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(7283..7329)
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 taacggccgc cagtgtgctg gaattcggct tttggtcgat accaagccat ttccgctgaa
       61 tatcgtggag ctcaccgcca gaatcggtgg ttgtggtgat gtacgtggcg aactccgttg
      121 tagtgcttgt ggtggcatcc gtggcgcggc cgcggtacca gatctttaaa tctagaggtg
      181 accacaacga cgcgcccgct ttgatcgggg acgtctgcgg ccgaccattt acgggtcttg
      241 ttgtcgttgg cggtcatggg ccgaacatac tcacccggat cggagggccg aggacaaggt
      301 cgaacgaggg gcatgacccg gtgcggggct tcttgcactc ggcataggcg agtgctaaga
      361 ataacgttgg cactcgcgac cggtgagtcg taggtcggga cggtgaggcc aggcccgtcg
      421 tcgcagcgag tggcagcgag gacaacttga gccgtccgtc gcgggcactg cgcccggcca
      481 gcgtaagtag cggggttgcc gtcacccggt gacccccggt ttcatccccg atccggagga
      541 atcacttcgc aatggccaag acaattgcgg atccagctgc aggctagcat gactggtgga
      601 cagcaaatgg gtcgggatct gtacgacgat gacgataagg atcgatgggg atccatggtg
      661 agcaagggcg aggaggtcat caaagagttc atgcgcttca aggtgcgcat ggagggctcc
      721 atgaacggcc acgagttcga gatcgagggc gagggcgagg gccgccccta cgagggcacc
      781 cagaccgcca agctgaaggt gaccaagggc ggccccctgc ccttcgcctg ggacatcctg
      841 tccccccagt tcatgtacgg ctccaaggcg tacgtgaagc accccgccga catccccgat
      901 tacaagaagc tgtccttccc cgagggcttc aagtgggagc gcgtgatgaa cttcgaggac
      961 ggcggtctgg tgaccgtgac ccaggactcc tccctgcagg acggcacgct gatctacaag
     1021 gtgaagatgc gcggcaccaa cttccccccc gacggccccg taatgcagaa gaagaccatg
     1081 ggctgggagg cctccaccga gcgcctgtac ccccgcgacg gcgtgctgaa gggcgagatc
     1141 caccaggccc tgaagctgaa ggacggcggc cactacctgg tggagttcaa gaccatctac
     1201 atggccaaga agcccgtgca actgcccggc tactactacg tggacaccaa gctggacatc
     1261 acctcccaca acgaggacta caccatcgtg gaacagtacg agcgctccga gggccgccac
     1321 cacctgttcc tggggcatgg caccggcagc accggcagcg gcagctccgg caccgcctcc
     1381 tccgaggaca acaacatggc cgtcatcaaa gagttcatgc gcttcaaggt gcgcatggag
     1441 ggctccatga acggccacga gttcgagatc gagggcgagg gcgagggccg cccctacgag
     1501 ggcacccaga ccgccaagct gaaggtgacc aagggcggcc ccctgccctt cgcctgggac
     1561 atcctgtccc cccagttcat gtacggctcc aaggcgtacg tgaagcaccc cgccgacatc
     1621 cccgattaca agaagctgtc cttccccgag ggcttcaagt gggagcgcgt gatgaacttc
     1681 gaggacggcg gtctggtgac cgtgacccag gactcctccc tgcaggacgg cacgctgatc
     1741 tacaaggtga agatgcgcgg caccaacttc ccccccgacg gccccgtaat gcagaagaag
     1801 accatgggct gggaggcctc caccgagcgc ctgtaccccc gcgacggcgt gctgaagggc
     1861 gagatccacc aggccctgaa gctgaaggac ggcggccact acctggtgga gttcaagacc
     1921 atctacatgg ccaagaagcc cgtgcaactg cccggctact actacgtgga caccaagctg
     1981 gacatcacct cccacaacga ggactacacc atcgtggaac agtacgagcg ctccgagggc
     2041 cgccaccacc tgttcctgta cggcatggac gagctgtaca agtaagaatt cgaagcttga
     2101 tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata
     2161 actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg
     2221 aactatatcc ggatctggcg taatagcgaa gaggcccgca ccgatcgccc ttcccaacag
     2281 ttgcgcagcc tgaatggcga atgggacgcg ccctgtagcg gcgcattaag cgcggcgggt
     2341 gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc
     2401 gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg
     2461 gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat
     2521 tagggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg
     2581 ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaaccct
     2641 atctcggtct attcttttga tttataaggg attttgccga tttcggccta ttggttaaaa
     2701 aatgagctga tttaacaaaa atttaacgcg aattttaaca aaatattaac gcttacaatt
     2761 taggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac
     2821 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa
     2881 aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat
     2941 tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc
     3001 agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga
     3061 gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg
     3121 cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc
     3181 agaatgactt ggttgagtac tctgcagctg gatccatgga tatctagatt taaagatctg
     3241 gtaccgcggc cgcgccacgg atgccaccac aacactacaa cggagttcgc cacgtacatc
     3301 accacaacca ccgattctgg cggtgagctc cccgatattc agcggaaatg gcttggtatc
     3361 gaccaagatt cgtagaaccc cgtctcgtct ggctggtatt caaaacgggc gcaacgaaac
     3421 acgcaacgag acaggcatgg cccaaaccag aaaactagcg tctaccagga cttttacctg
     3481 tccgacccgt tgcaacggaa ccccccacgg aacccccgcg acacccgctc cccaattgcg
     3541 ttagaacagc ggtggattgt cggcttcgtt gtgggccttt tgagccgctt cctgttctgc
     3601 cgcacgctct ttcctcgccc gatagccgag tcgcttaacg gtgtccagat gcagcccgaa
     3661 atgtttggcc gtttgcggcc aagagtggcc ctcgtcgtcg tgataggcgc ggatgcgttc
     3721 gcggcgtgca gcctgctcgg cgagccactc gctgcgttcc tgcgccacga gccggacgac
     3781 gtggcgttcg gatagtccgg tgattcgagc gccttcggcg gcggtcacgc gccgcttttt
     3841 gcggacagtc ggctgccggt tgtagccgtc gctgtagccg tcgctgtagc cgtcgctcat
     3901 agcaatgcct ccatgcatgg ctgacgcgga ctttgcgcgc cgcgcaactg tgctcgccgc
     3961 cgtgcgcgct gctgcgccct tccgcgagat ggccgactgg cgcgcactga gtgtggcctc
     4021 gtagaccacg atcccgtccg cccaaatgcg cgacttggtt gtgatccaac gccaaatgct
     4081 gttggcgatg gcgcggacct cgctgtccgg tagcggtccg ggacacacgt cgttgcacgg
     4141 aaattcggcg tttcgcgcgt ggcactcggc atagatcgcg cggccgagtc cgtccacgtt
     4201 ccgggtcggc aggtagatcc gcatgagggc gggacgatag gcccacaacc tgacggaatc
     4261 gaacagtgcg caattccgcc ctagcggcgt cggagccgct ttgtacgtgg tctgctgacg
     4321 ccagcgcggc ggtggcatgt tcgcgccgag ctcggcctcg atgtggctga gtgtgtagag
     4381 atctgagtgg agccattccg tttcccaggc gatgtggccg gggtttttgg tcatgaggcc
     4441 tgagtaactg cggtcgccat cgacggcgcg ccgaaggcct tcggcgcacg ccgccatgta
     4501 tgcgagcggc ttacgccgcg cgtattcggt gcgtggaaca ggggcgttga gtgcccacac
     4561 tgcgtgtgcg tggccgttgg cgcgattgcc cacgatcgcg ttgggcagcg gatgggaccc
     4621 ccgggcgctg agcgctcgga gcgctgcgtc tggatggtct acgtccacga ccagcaggtt
     4681 tgccagcgct gttgggttcg cctcgatgta ccggcggcct agggccgacg cgcggctttg
     4741 gcggtagatc ccctcgagca gatcgtcgct tgccagcggc cagtacggca gccagagctg
     4801 ctcaaattcg tcggcgacgt ggctcacgct tggtagtaga ccacgattaa tcaccggtgt
     4861 atggtccgac acgagctcca agtcagatat ttcgctgagg ggccacccca caactgcaca
     4921 ctcccccgct ctcccgtcga gccctggtgg tggaacacca gcgacagccg agcaccccca
     4981 accacctgta ccaaccagga ggaacacatg cgtcgtttcg aggacgtttc cgggccgctg
     5041 agagccgctg tggcggccgt acacgccgcc ttagacccgt tagaccccct gccgcctgaa
     5101 tgcgcgggta cgagccacac agcgcccgaa cttacggagc tggtgggctc acgcgtgcgg
     5161 ccgcacgcgt tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg
     5221 gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc
     5281 cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc
     5341 ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga
     5401 ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc
     5461 ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcaa
     5521 tgctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg
     5581 cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc
     5641 aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga
     5701 gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact
     5761 agaaggacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt
     5821 ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag
     5881 cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg
     5941 tctgacgctc agtggaacta gagcatgaga ttatcaaaaa ggatcttcac ctagatcctt
     6001 ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac
     6061 agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc
     6121 atagttgcct gactccccga cgtggccgac cagcccgtca tcgtcaacgc ctgaccgcgg
     6181 tgcggacagg ccgtgtcgcg accggccgtg cggaattaag ccggcccgta ccctgtgaat
     6241 agaggtccgc tgtgacacaa gaatccctgt tacttctcga ccgtattgat tcggatgatt
     6301 cctacgcgag cctgcggaac gaccaggaat tctgggagcc gctggcccgc cgagccctgg
     6361 aggagctcgg gctgccggtg ccgccggtgc tgcgggtgcc cggcgagagc accaaccccg
     6421 tactggtcgg cgagcccgac ccggtgatca agctgttcgg cgagcactgg tgcggtccgg
     6481 agagcctcgc gtcggagtcg gaggcgtacg cggtcctggc ggacgccccg gtgccggtgc
     6541 cccgcctcct cggccgcggc gagctgcggc ccggcaccgg agcctggccg tggccctacc
     6601 tggtgatgag ccggatgacc ggcaccacct ggcggtccgc gatggacggc acgaccgacc
     6661 ggaacgcgct gctcgccctg gcccgcgaac tcggccgggt gctcggccgg ctgcacaggg
     6721 tgccgctgac cgggaacacc gtgctcaccc cccattccga ggtcttcccg aactgctgcg
     6781 ggacgccgcg cggcgccgct gaggaccacc gcgggtgggg ctacctctgg ccccggctgc
     6841 tggaccgcct ggaggactgg ctgccggacg tggacacgct gctggccggc cgcgaacccc
     6901 ggttcgtcca cggcgacctg cacgggacca acatcttcgt ggacctggcc gcgaccgagg
     6961 tcaccgggat cgtcgacttc accgacgtct atgcgggaga ctcccgctac agcctggtgc
     7021 aactgcatct caacgccttc cggggcgacc gcgagatcct ggccgcgctg ctcgacgggg
     7081 cgcagtggaa gcggaccgag gacttcgccc gcgaactgct cgccttcacc ttcctgcacg
     7141 acttcgaggt gttcgaggag accccgctgg atctctccgg cttcaccgat ccggaggaac
     7201 tggcgcagtt cctctggggg ccgccggaca ccgcccccgg cgcctgacgc cccctctagc
     7261 tgatcaccgc ggccatgatg gcataaaacg aaaggcccag tctttcgact gagcctttcg
     7321 ttttatttga tgcctggcag tcgatcgtac gctagttaac tacgtcgaca tcgataagct
     7381 tggtaccgag ctcggatcca ctag
//
