LOCUS       sequence-304745-        6707 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  mCherry is expressed under the mycobacterial phsp60 heat shock 
            promoter for in vivo and in vitro detection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6707)
  AUTHORS   Kong Y, Yang D, Cirillo SL, Li S, Akin A, Francis KP, Maloney T, 
            Cirillo JD
  TITLE     Application of Fluorescent Protein Expressing Strains to Evaluation 
            of Anti-Tuberculosis Therapeutic Efficacy In Vitro and In Vivo.
  JOURNAL   PLoS One. 2016 Mar 2;11(3):e0149972. doi: 
            10.1371/journal.pone.0149972. eCollection 2016.
  PUBMED    26934495
REFERENCE   2  (bases 1 to 6707)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2016-03-2"; volume: "11(3):e0149972. doi"; pages: " 
            10.1371/journal.pone.0149972. eCollection 2016"
COMMENT     Sequence Label: pJDC124
FEATURES             Location/Qualifiers
     source          1..6707
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             583..612
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="ASMTGGQQMG"
     CDS             join(616..624,625..639)
                     /codon_start=1
                     /product="Xpress(TM) epitope tag, including an enterokinase
                     recognition and cleavage site"
                     /label=Xpress(TM) tag
                     /translation="DLYDDDDK"
     regulatory      658..667
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             664..1374
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(809..827)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     1077..1096
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     complement(1451..1469)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      1465..1512
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     rep_origin      1609..2064
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1696..1715)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     1906..1927
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        2090..2194
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(2413..2432)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      complement(4628..5216)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4708..4727)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(6586..6632)
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 taacggccgc cagtgtgctg gaattcggct tttggtcgat accaagccat ttccgctgaa
       61 tatcgtggag ctcaccgcca gaatcggtgg ttgtggtgat gtacgtggcg aactccgttg
      121 tagtgcttgt ggtggcatcc gtggcgcggc cgcggtacca gatctttaaa tctagaggtg
      181 accacaacga cgcgcccgct ttgatcgggg acgtctgcgg ccgaccattt acgggtcttg
      241 ttgtcgttgg cggtcatggg ccgaacatac tcacccggat cggagggccg aggacaaggt
      301 cgaacgaggg gcatgacccg gtgcggggct tcttgcactc ggcataggcg agtgctaaga
      361 ataacgttgg cactcgcgac cggtgagtcg taggtcggga cggtgaggcc aggcccgtcg
      421 tcgcagcgag tggcagcgag gacaacttga gccgtccgtc gcgggcactg cgcccggcca
      481 gcgtaagtag cggggttgcc gtcacccggt gacccccggt ttcatccccg atccggagga
      541 atcacttcgc aatggccaag acaattgcgg atccagctgc aggctagcat gactggtgga
      601 cagcaaatgg gtcgggatct gtacgacgat gacgataagg atccccgggt accggtcgcc
      661 accatggtga gcaagggcga ggaggataac atggccatca tcaaggagtt catgcgcttc
      721 aaggtgcaca tggagggctc cgtgaacggc cacgagttcg agatcgaggg cgagggcgag
      781 ggccgcccct acgagggcac ccagaccgcc aagctgaagg tgaccaaggg tggccccctg
      841 cccttcgcct gggacatcct gtcccctcag ttcatgtacg gctccaaggc ctacgtgaag
      901 caccccgccg acatccccga ctacttgaag ctgtccttcc ccgagggctt caagtgggag
      961 cgcgtgatga acttcgagga cggcggcgtg gtgaccgtga cccaggactc ctccctgcag
     1021 gacggcgagt tcatctacaa ggtgaagctg cgcggcacca acttcccctc cgacggcccc
     1081 gtaatgcaga agaagaccat gggctgggag gcctcctccg agcggatgta ccccgaggac
     1141 ggcgccctga agggcgagat caagcagagg ctgaagctga aggacggcgg ccactacgac
     1201 gctgaggtca agaccaccta caaggccaag aagcccgtgc agctgcccgg cgcctacaac
     1261 gtcaacatca agttggacat cacctcccac aacgaggact acaccatcgt ggaacagtac
     1321 gaacgcgccg agggccgcca ctccaccggc ggcatggacg agctgtacaa gtagcggccg
     1381 cgactctaga attcgaagct tgatccggct gctaacaaag cccgaaagga agctgagttg
     1441 gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg
     1501 aggggttttt tgctgaaagg aggaactata tccggatctg gcgtaatagc gaagaggccc
     1561 gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg cgaatgggac gcgccctgta
     1621 gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca
     1681 gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct
     1741 ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt gctttacggc
     1801 acctcgaccc caaaaaactt gattagggtg atggttcacg tagtgggcca tcgccctgat
     1861 agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga ctcttgttcc
     1921 aaactggaac aacactcaac cctatctcgg tctattcttt tgatttataa gggattttgc
     1981 cgatttcggc ctattggtta aaaaatgagc tgatttaaca aaaatttaac gcgaatttta
     2041 acaaaatatt aacgcttaca atttaggtgg cacttttcgg ggaaatgtgc gcggaacccc
     2101 tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg
     2161 ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc
     2221 ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt
     2281 gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct
     2341 caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac
     2401 ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc aagagcaact
     2461 cggtcgccgc atacactatt ctcagaatga cttggttgag tactctgcag ctggatccat
     2521 ggatatctag atttaaagat ctggtaccgc ggccgcgcca cggatgccac cacaacacta
     2581 caacggagtt cgccacgtac atcaccacaa ccaccgattc tggcggtgag ctccccgata
     2641 ttcagcggaa atggcttggt atcgaccaag attcgtagaa ccccgtctcg tctggctggt
     2701 attcaaaacg ggcgcaacga aacacgcaac gagacaggca tggcccaaac cagaaaacta
     2761 gcgtctacca ggacttttac ctgtccgacc cgttgcaacg gaacccccca cggaaccccc
     2821 gcgacacccg ctccccaatt gcgttagaac agcggtggat tgtcggcttc gttgtgggcc
     2881 ttttgagccg cttcctgttc tgccgcacgc tctttcctcg cccgatagcc gagtcgctta
     2941 acggtgtcca gatgcagccc gaaatgtttg gccgtttgcg gccaagagtg gccctcgtcg
     3001 tcgtgatagg cgcggatgcg ttcgcggcgt gcagcctgct cggcgagcca ctcgctgcgt
     3061 tcctgcgcca cgagccggac gacgtggcgt tcggatagtc cggtgattcg agcgccttcg
     3121 gcggcggtca cgcgccgctt tttgcggaca gtcggctgcc ggttgtagcc gtcgctgtag
     3181 ccgtcgctgt agccgtcgct catagcaatg cctccatgca tggctgacgc ggactttgcg
     3241 cgccgcgcaa ctgtgctcgc cgccgtgcgc gctgctgcgc ccttccgcga gatggccgac
     3301 tggcgcgcac tgagtgtggc ctcgtagacc acgatcccgt ccgcccaaat gcgcgacttg
     3361 gttgtgatcc aacgccaaat gctgttggcg atggcgcgga cctcgctgtc cggtagcggt
     3421 ccgggacaca cgtcgttgca cggaaattcg gcgtttcgcg cgtggcactc ggcatagatc
     3481 gcgcggccga gtccgtccac gttccgggtc ggcaggtaga tccgcatgag ggcgggacga
     3541 taggcccaca acctgacgga atcgaacagt gcgcaattcc gccctagcgg cgtcggagcc
     3601 gctttgtacg tggtctgctg acgccagcgc ggcggtggca tgttcgcgcc gagctcggcc
     3661 tcgatgtggc tgagtgtgta gagatctgag tggagccatt ccgtttccca ggcgatgtgg
     3721 ccggggtttt tggtcatgag gcctgagtaa ctgcggtcgc catcgacggc gcgccgaagg
     3781 ccttcggcgc acgccgccat gtatgcgagc ggcttacgcc gcgcgtattc ggtgcgtgga
     3841 acaggggcgt tgagtgccca cactgcgtgt gcgtggccgt tggcgcgatt gcccacgatc
     3901 gcgttgggca gcggatggga cccccgggcg ctgagcgctc ggagcgctgc gtctggatgg
     3961 tctacgtcca cgaccagcag gtttgccagc gctgttgggt tcgcctcgat gtaccggcgg
     4021 cctagggccg acgcgcggct ttggcggtag atcccctcga gcagatcgtc gcttgccagc
     4081 ggccagtacg gcagccagag ctgctcaaat tcgtcggcga cgtggctcac gcttggtagt
     4141 agaccacgat taatcaccgg tgtatggtcc gacacgagct ccaagtcaga tatttcgctg
     4201 aggggccacc ccacaactgc acactccccc gctctcccgt cgagccctgg tggtggaaca
     4261 ccagcgacag ccgagcaccc ccaaccacct gtaccaacca ggaggaacac atgcgtcgtt
     4321 tcgaggacgt ttccgggccg ctgagagccg ctgtggcggc cgtacacgcc gccttagacc
     4381 cgttagaccc cctgccgcct gaatgcgcgg gtacgagcca cacagcgccc gaacttacgg
     4441 agctggtggg ctcacgcgtg cggccgcacg cgttgcgctc ggtcgttcgg ctgcggcgag
     4501 cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag
     4561 gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc
     4621 tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc
     4681 agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc
     4741 tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt
     4801 cgggaagcgt ggcgctttct caatgctcac gctgtaggta tctcagttcg gtgtaggtcg
     4861 ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat
     4921 ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag
     4981 ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt
     5041 ggtggcctaa ctacggctac actagaagga cagtatttgg tatctgcgct ctgctgaagc
     5101 cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta
     5161 gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag
     5221 atcctttgat cttttctacg gggtctgacg ctcagtggaa ctagagcatg agattatcaa
     5281 aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta
     5341 tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag
     5401 cgatctgtct atttcgttca tccatagttg cctgactccc cgacgtggcc gaccagcccg
     5461 tcatcgtcaa cgcctgaccg cggtgcggac aggccgtgtc gcgaccggcc gtgcggaatt
     5521 aagccggccc gtaccctgtg aatagaggtc cgctgtgaca caagaatccc tgttacttct
     5581 cgaccgtatt gattcggatg attcctacgc gagcctgcgg aacgaccagg aattctggga
     5641 gccgctggcc cgccgagccc tggaggagct cgggctgccg gtgccgccgg tgctgcgggt
     5701 gcccggcgag agcaccaacc ccgtactggt cggcgagccc gacccggtga tcaagctgtt
     5761 cggcgagcac tggtgcggtc cggagagcct cgcgtcggag tcggaggcgt acgcggtcct
     5821 ggcggacgcc ccggtgccgg tgccccgcct cctcggccgc ggcgagctgc ggcccggcac
     5881 cggagcctgg ccgtggccct acctggtgat gagccggatg accggcacca cctggcggtc
     5941 cgcgatggac ggcacgaccg accggaacgc gctgctcgcc ctggcccgcg aactcggccg
     6001 ggtgctcggc cggctgcaca gggtgccgct gaccgggaac accgtgctca ccccccattc
     6061 cgaggtcttc ccgaactgct gcgggacgcc gcgcggcgcc gctgaggacc accgcgggtg
     6121 gggctacctc tggccccggc tgctggaccg cctggaggac tggctgccgg acgtggacac
     6181 gctgctggcc ggccgcgaac cccggttcgt ccacggcgac ctgcacggga ccaacatctt
     6241 cgtggacctg gccgcgaccg aggtcaccgg gatcgtcgac ttcaccgacg tctatgcggg
     6301 agactcccgc tacagcctgg tgcaactgca tctcaacgcc ttccggggcg accgcgagat
     6361 cctggccgcg ctgctcgacg gggcgcagtg gaagcggacc gaggacttcg cccgcgaact
     6421 gctcgccttc accttcctgc acgacttcga ggtgttcgag gagaccccgc tggatctctc
     6481 cggcttcacc gatccggagg aactggcgca gttcctctgg gggccgccgg acaccgcccc
     6541 cggcgcctga cgccccctct agctgatcac cgcggccatg atggcataaa acgaaaggcc
     6601 cagtctttcg actgagcctt tcgttttatt tgatgcctgg cagtcgatcg tacgctagtt
     6661 aactacgtcg acatcgataa gcttggtacc gagctcggat ccactag
//
