LOCUS       sequence-304753-        4255 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses the RT-Abi-P2 defense system.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4255)
  AUTHORS   Gao L, Altae-Tran H, Bohning F, Makarova KS, Segel M, Schmid-Burgk 
            JL, Koob J, Wolf YI, Koonin EV, Zhang F
  TITLE     Diverse enzymatic activities mediate antiviral immunity in 
            prokaryotes.
  JOURNAL   Science. 2020 Aug 28;369(6507):1077-1084. doi: 
            10.1126/science.aba0372.
  PUBMED    32855333
REFERENCE   2  (bases 1 to 4255)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1126/science"; 
            journalName: "Science"; date: "2020-08-28- 28"; volume: "369"; 
            issue: "6507"; pages: "1077-1084"
COMMENT     Sequence Label: pLG004 (RT-Abi-P2)
FEATURES             Location/Qualifiers
     source          1..4255
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      2035..2082
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     CDS             complement(2302..2961)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     2876..2895
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(2962..3064)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     primer_bind     complement(3456..3473)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3590..4135)
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
     primer_bind     complement(3670..3689)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgagatc
       61 ccggtgccta atgagtgagc taacttacat taattgcgtt gcgcaacatc ccgtcatcat
      121 gccatcacga cgcgctgaga cgctgaaaaa ataaaatcag caccaccgtc agcgcgcagt
      181 gctttccccg cctcgcccgc ccgcttcatg agacggtttt aatgcagttg cattatgtcc
      241 cgctcctcag tgctgcgctc catcctgatt acaaaaaccg ttatcaaaaa cacatgcaaa
      301 tagacgcagt caaatgcgct accgcctctc gcaatacctt caatttcatg ataaaaaaca
      361 tcatccctaa caagagcatt atcctcatga aaaaagtata tgaactaacc agtgaagaag
      421 cactgtcata ttttcttcgc catgactcct acacaacatt agaattaccg gcttatatta
      481 atttcaccac attattaaat gatattaatt catctatcca taacaaaaaa attaaaattg
      541 aaccaaccgc caaggagctg atgggtaaag atatcaatta tgaggtgctt gtcagtaaag
      601 atggtctata tagctggcgt aggataacac ttatcaatcc cctttattat gtctacttct
      661 gtagaaaaat cacagcacca gcaacctggg aaatcataac agaaaaattc aaatcttttg
      721 aatcaaacga cctttttaca tgttcaagca tccccgtcag aaaagacaac tcgtcaaaca
      781 ttgctgcgtc tgtaatgaat tggtgggaag attttgaaca aaaaagcctt gcccttgctc
      841 ttgaatacga attcatgttc agcactgaca tctcaaactt ctacccatca atatatactc
      901 atagttttga atgggtattc atatcaaaag aagaggcaaa gaagaaaaaa agcaaaaata
      961 acccaggggg attaattgac agccacattc aaatgatgat gaacaaccag acaaatggta
     1021 ttccactcgg cagcacattg atggatacat ttgctgagct tatcttgggt caaatcgata
     1081 tagaattaag aaaaaaaact aacgaactca aaataataaa ctacaaggta gtacgctacc
     1141 gtgatgatta ccggatcttc tctaatagca aagatgattt agacataata tcaaaatgtt
     1201 tagtcaatgt attgggcgat tttggtttag atctaaactc aaaaaaaact gaactatatg
     1261 aagacatcat acttcattcg ttgaaacaag ctaaaaaaga ctacatcaaa gaaaaaagac
     1321 ataagtcact ccagaaaatg ctctattcaa tatatttatt ttcacttaaa catccaaact
     1381 cgaaaacaac cgttagatat ctaaatgatt ttcttaggaa tttatttaag cgaaagacaa
     1441 ttaaagataa cggccaacag gttgatgcta tgcttggtat tatttcaagc atcatggcaa
     1501 aaaaccctac aacgtaccca gtaggaacgg caattttctc aaaactcctc agttttcttt
     1561 atggtgatga cacccaaaaa aaattaacaa agctagaaca actccataaa aaactggata
     1621 aacaacccaa tacagaaatg cttgacatat ggtttcagcg aactcaagca aaaataaacc
     1681 tagagtggaa taaatcttat aagtcagctc tatgcgtccg tataaatgat gaactcacaa
     1741 aagagaaaac attttctgta aataatttat ggaatattga ctggatccaa ggaaaagaaa
     1801 caagccccaa taaagccaaa atattatcct tgctaagaaa aacaaaaatc gttgacacag
     1861 ataaatttga taaaatggat gacaatataa cacctgaaga agttaatcta ttctttaaag
     1921 agcacagcaa ttaatatccc aaagccatgt tagtaacata acatggcttt tttaaatcac
     1981 tcattatcag ttatcaagaa cgaacataac attctattcc gaggagcgca ataactagca
     2041 taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaacc tcaggcattt
     2101 gagaagcaca cggtcacact gcttccggta gtcaataaac cggtaaacca gcaatagaca
     2161 taagcggcta tttaacgacc ctgccctgaa ccgacgaccg ggtcgaattt gctttcgaat
     2221 ttctgccatt catccgctta ttatcactta ttcaggcgta gcaccaggcg tttaagggca
     2281 ccaataactg ccttaaaaaa attacgcccc gccctgccac tcatcgcaat actgttgtaa
     2341 ttcatttaac attctgccga catggaagcc atcacagacg gcatgatgaa cctgaatcgc
     2401 cagcggcatc agcaccttgt cgccttgcgt ataatatttg cccatagtga aaacgggggc
     2461 gaagaagttg tccatattgg ccacgtttaa atcaaaactg gtgaaactca cccagggatt
     2521 ggctgagacg aaaaacatat tctcaataaa ccctttaggg aaataggcca ggttttcacc
     2581 gtaacacgcc acatcttgcg aatatatgtg tagaaactgc cggaaatcgt cgtggtattc
     2641 actccagagc gatgaaaacg tttcagtttg ctcatggaaa acggtgtaac aagggtgaac
     2701 actatcccat atcaccagct caccgtcttt catcgccata cggaactctg gatgagcatt
     2761 catcaggcgg gcaagaatgt gaataaaggc cggataaaac ttgtgcttat ttttctttac
     2821 ggtctttaaa aaggccgtaa tatccagctg aacggtctgg ttataggtac attgagcaac
     2881 tgactgaaat gcctcaaaat gttctttacg atgccattgg gatatatcaa cggtggtata
     2941 tccagtgatt tttttctcca ttttagcttc cttagctcct gaaaatctcg ataactcaaa
     3001 aaatacgccc ggtagtgatc ttatttcatt atggtgaaag ttggaacctc ttacgtgccg
     3061 atcaacgtct cattttcgcc aaaagttggc ccagggcttc ccggtatcaa cagggacacc
     3121 aggatttatt tattctgcga agtgatcttc cgtcacaggt atttattcgg cgcaaagtgc
     3181 gtcgggtgat gctgccaact tactgattta gtgtatgatg gtgtttttga ggtgctccag
     3241 tggcttctgt ttctatcagc tgtccctcct gttcagctac tgacggggtg gtgcgtaacg
     3301 gcaaaagcac cgccggacat cagcgctagc ggagtgtata ctggcttact atgttggcac
     3361 tgatgagggt gtcagtgaag tgcttcatgt ggcaggagaa aaaaggctgc accggtgcgt
     3421 cagcagaata tgtgatacag gatatattcc gcttcctcgc tcactgactc gctacgctcg
     3481 gtcgttcgac tgcggcgagc ggaaatggct tacgaacggg gcggagattt cctggaagat
     3541 gccaggaaga tacttaacag ggaagtgaga gggccgcggc aaagccgttt ttccataggc
     3601 tccgcccccc tgacaagcat cacgaaatct gacgctcaaa tcagtggtgg cgaaacccga
     3661 caggactata aagataccag gcgtttcccc ctggcggctc cctcgtgcgc tctcctgttc
     3721 ctgcctttcg gtttaccggt gtcattccgc tgttatggcc gcgtttgtct cattccacgc
     3781 ctgacactca gttccgggta ggcagttcgc tccaagctgg actgtatgca cgaacccccc
     3841 gttcagtccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggaaaga
     3901 catgcaaaag caccactggc agcagccact ggtaattgat ttagaggagt tagtcttgaa
     3961 gtcatgcgcc ggttaaggct aaactgaaag gacaagtttt ggtgactgcg ctcctccaag
     4021 ccagttacct cggttcaaag agttggtagc tcagagaacc ttcgaaaaac cgccctgcaa
     4081 ggcggttttt tcgttttcag agcaagagat tacgcgcaga ccaaaacgat ctcaagaaga
     4141 tcatcttatt aatcagataa aatatttcta gatttcagtg caatttatct cttcaaatgt
     4201 agcacctgaa gtcagcccca tacgatataa gttgtaattc tcatgttagt catgc
//
