LOCUS       sequence-304760-        4114 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses the DRT type 2 defense system.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4114)
  AUTHORS   Gao L, Altae-Tran H, Bohning F, Makarova KS, Segel M, Schmid-Burgk 
            JL, Koob J, Wolf YI, Koonin EV, Zhang F
  TITLE     Diverse enzymatic activities mediate antiviral immunity in 
            prokaryotes.
  JOURNAL   Science. 2020 Aug 28;369(6507):1077-1084. doi: 
            10.1126/science.aba0372.
  PUBMED    32855333
REFERENCE   2  (bases 1 to 4114)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1126/science"; 
            journalName: "Science"; date: "2020-08-28- 28"; volume: "369"; 
            issue: "6507"; pages: "1077-1084"
COMMENT     Sequence Label: pLG010 (DRT type 2)
FEATURES             Location/Qualifiers
     source          1..4114
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      1894..1941
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     CDS             complement(2161..2820)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     2735..2754
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(2821..2923)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     primer_bind     complement(3315..3332)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3449..3994)
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
     primer_bind     complement(3529..3548)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgagatc
       61 ccggtgccta atgagtgagc taacttacat taattgcgtt gcgcaacacg atataaaacc
      121 atctcattgc ttgctgggtt aactgagttg ctgaattttt ttctagaatt tcgcaaaatt
      181 taataggtaa accttgtttt tttaaattta cgatgatata aaaataatgc cctaaacaaa
      241 ggtttagggg tattgtacag gttgtcaagc ctcccacagg tcttggtgaa accaatcact
      301 gtgacgacgg taagcaacac ttggatgata ttcataattg actccacgct actgattaca
      361 ttatacagca tatctaacat ttgcggcgag gttcacaatt tgtatttagg tactgattgt
      421 ggatgagaag gttggagaaa gaccacttgg ttaagccgga ggatgtgtcc tagaattgtc
      481 gctattctgt catcctccgg ttttgctaat ttcattcagg gaatataatg aataatgatg
      541 attacccatg gttcagaaaa cgtggttatt tgcatttcga tgaacctgtt tcattaaaaa
      601 aagcggttaa atatgtttcc tctccagaaa aaataataaa acattctttt ctgccatttt
      661 taagctttga agtaaaatcg tttaaaatca aaaaagacaa atcaacaaaa caattaagta
      721 aaactgaaaa attaagacct attgcctatt cctcacattt ggatagtcat atttatgcat
      781 tttacgcaga atatcttact ggacattatg aattattgat ccaagaaaac aatttacacg
      841 agaacatcct tgccttcaga tctttaaata aaagcaatat agaatttgcc aagagagcat
      901 ttgatacaat tactgaaatg ggtgagtgta gcgctgttgc attagatctt tctggttttt
      961 ttgacaattt agatcatcaa attttgaaac accagtggtg caaagttatt gggactgaag
     1021 cgttgccgca agaccatttt gccatataca aaagtataac aagatattct aaagttgata
     1081 aaaatagagc gtatgagatt ttaggtatat caaagaataa ccccaagtat aatagacgca
     1141 agatctgcac ccctgttgat tttagaaata agattagaaa aaatggtctt attatagtta
     1201 ataattccca aaaaggtata ccccaaggct cgccaattag tgctctactt tcaaatatat
     1261 atatgcttga ctttgatatt gaaatgagag attacgcgca ggaacgtggt ggccattatt
     1321 atcgctattg tgatgatatg ctattcattg taccaactaa gtataataaa actctagcag
     1381 gtgatgtagc ccagcggatt aagcatctta aggtagaact caatactaag aaaactgaga
     1441 ttcgagattt tatatacaaa gacagtacct tagtggcaaa tatgccttta cagtatcttg
     1501 ggtttatttt tgatgggagt aatatattat tacgttcatc ttctctcgca agatattcgg
     1561 aacgaatgaa aagaggtgtc cgcttagcaa aagctacaat ggacagcaag aataggatta
     1621 gagaaaataa aggtgaagct ttaaaagctt tatttaagaa aaaattatat gccagatatt
     1681 cacatattgg aagaaggaat tttttgactt atggttatcg cgccgcgaag atcatgaatt
     1741 cgaaagctat aaaaagacag ttaaaaccat tgcagaaaag attggaaaat gaaatactaa
     1801 aataaatatt tgctggcccg aatcatacag ggccacaata cagttgaaaa caagctataa
     1861 taaacaacat ctaattttta tataccgcaa taactagcat aaccccttgg ggcctctaaa
     1921 cgggtcttga ggggtttttt gctgaaacct caggcatttg agaagcacac ggtcacactg
     1981 cttccggtag tcaataaacc ggtaaaccag caatagacat aagcggctat ttaacgaccc
     2041 tgccctgaac cgacgaccgg gtcgaatttg ctttcgaatt tctgccattc atccgcttat
     2101 tatcacttat tcaggcgtag caccaggcgt ttaagggcac caataactgc cttaaaaaaa
     2161 ttacgccccg ccctgccact catcgcaata ctgttgtaat tcatttaaca ttctgccgac
     2221 atggaagcca tcacagacgg catgatgaac ctgaatcgcc agcggcatca gcaccttgtc
     2281 gccttgcgta taatatttgc ccatagtgaa aacgggggcg aagaagttgt ccatattggc
     2341 cacgtttaaa tcaaaactgg tgaaactcac ccagggattg gctgagacga aaaacatatt
     2401 ctcaataaac cctttaggga aataggccag gttttcaccg taacacgcca catcttgcga
     2461 atatatgtgt agaaactgcc ggaaatcgtc gtggtattca ctccagagcg atgaaaacgt
     2521 ttcagtttgc tcatggaaaa cggtgtaaca agggtgaaca ctatcccata tcaccagctc
     2581 accgtctttc atcgccatac ggaactctgg atgagcattc atcaggcggg caagaatgtg
     2641 aataaaggcc ggataaaact tgtgcttatt tttctttacg gtctttaaaa aggccgtaat
     2701 atccagctga acggtctggt tataggtaca ttgagcaact gactgaaatg cctcaaaatg
     2761 ttctttacga tgccattggg atatatcaac ggtggtatat ccagtgattt ttttctccat
     2821 tttagcttcc ttagctcctg aaaatctcga taactcaaaa aatacgcccg gtagtgatct
     2881 tatttcatta tggtgaaagt tggaacctct tacgtgccga tcaacgtctc attttcgcca
     2941 aaagttggcc cagggcttcc cggtatcaac agggacacca ggatttattt attctgcgaa
     3001 gtgatcttcc gtcacaggta tttattcggc gcaaagtgcg tcgggtgatg ctgccaactt
     3061 actgatttag tgtatgatgg tgtttttgag gtgctccagt ggcttctgtt tctatcagct
     3121 gtccctcctg ttcagctact gacggggtgg tgcgtaacgg caaaagcacc gccggacatc
     3181 agcgctagcg gagtgtatac tggcttacta tgttggcact gatgagggtg tcagtgaagt
     3241 gcttcatgtg gcaggagaaa aaaggctgca ccggtgcgtc agcagaatat gtgatacagg
     3301 atatattccg cttcctcgct cactgactcg ctacgctcgg tcgttcgact gcggcgagcg
     3361 gaaatggctt acgaacgggg cggagatttc ctggaagatg ccaggaagat acttaacagg
     3421 gaagtgagag ggccgcggca aagccgtttt tccataggct ccgcccccct gacaagcatc
     3481 acgaaatctg acgctcaaat cagtggtggc gaaacccgac aggactataa agataccagg
     3541 cgtttccccc tggcggctcc ctcgtgcgct ctcctgttcc tgcctttcgg tttaccggtg
     3601 tcattccgct gttatggccg cgtttgtctc attccacgcc tgacactcag ttccgggtag
     3661 gcagttcgct ccaagctgga ctgtatgcac gaaccccccg ttcagtccga ccgctgcgcc
     3721 ttatccggta actatcgtct tgagtccaac ccggaaagac atgcaaaagc accactggca
     3781 gcagccactg gtaattgatt tagaggagtt agtcttgaag tcatgcgccg gttaaggcta
     3841 aactgaaagg acaagttttg gtgactgcgc tcctccaagc cagttacctc ggttcaaaga
     3901 gttggtagct cagagaacct tcgaaaaacc gccctgcaag gcggtttttt cgttttcaga
     3961 gcaagagatt acgcgcagac caaaacgatc tcaagaagat catcttatta atcagataaa
     4021 atatttctag atttcagtgc aatttatctc ttcaaatgta gcacctgaag tcagccccat
     4081 acgatataag ttgtaattct catgttagtc atgc
//
