LOCUS       sequence-304764-        7860 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses the RADAR defense system.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7860)
  AUTHORS   Gao L, Altae-Tran H, Bohning F, Makarova KS, Segel M, Schmid-Burgk 
            JL, Koob J, Wolf YI, Koonin EV, Zhang F
  TITLE     Diverse enzymatic activities mediate antiviral immunity in 
            prokaryotes.
  JOURNAL   Science. 2020 Aug 28;369(6507):1077-1084. doi: 
            10.1126/science.aba0372.
  PUBMED    32855333
REFERENCE   2  (bases 1 to 7860)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1126/science"; 
            journalName: "Science"; date: "2020-08-28- 28"; volume: "369"; 
            issue: "6507"; pages: "1077-1084"
COMMENT     Sequence Label: pLG014 (RADAR)
FEATURES             Location/Qualifiers
     source          1..7860
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      5640..5687
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     CDS             complement(5907..6566)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     6481..6500
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(6567..6669)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     primer_bind     complement(7061..7078)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7195..7740)
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
     primer_bind     complement(7275..7294)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgagatc
       61 ccggtgccta atgagtgagc taacttacat taattgcgtt gcgcagagga tttatgcaca
      121 aaatcctgat gcgaaatgtt ttcaaaaatt gtcaggttaa cgttcctgca gatctttgcg
      181 ttacatgtca tttctggatc ctttcccgac aggttaggtt gtgattgata tgatgcccat
      241 ctctcatttt agtgatcgtt atccctttat aaacaggagt ttatatgtta tctatatgca
      301 atagacttaa atcgatatac gtgcgcagct tacgattcac ctctctactt actatttaag
      361 gaaaagagtg aggggagaat tgattttcat taagatatta tgagagaatt atgactagtg
      421 aaatagtgtt aaatcttgat ttcccagaat ataaggatga tttttgtact gatagcattg
      481 atgagcaaga taatgagttg tggcagcaac aggccaataa aaagctactt tcgtttctcg
      541 aggtgatggg ggaggaagca agacgatata aagaaaataa ttcccgtagt acgcatccac
      601 attataagac attgagtagt tatcaccatg caatctttat cagtggcgcg cggggggcgg
      661 ggaaaactgt tttcatgaga aatgccagat ttagctggca aaaacattat aataaagatc
      721 taaaacgccc taagctatat tttattgatg tgattgaccc gacgctattg aatattgatg
      781 accgtttttc tgaagtcatt atcgcttcaa tatatgctac ggtagaaaag cggatgaagc
      841 aacctgatat tgcgcagaat atcaaagata attttattaa ttcgcttaag acgttgtccg
      901 gtgcattagg taaatcaaaa gattatgatg aatatagggg cattgatcgt attcaaaaat
      961 atcgttctgg aatccacctt gaaaaatatt tccatcagtt cttgatttca agcgttgagt
     1021 tactggattg cgatgcgctg gttttgccga ttgatgatgt tgatatgaaa atagataacg
     1081 cttttggtgt tctggacgat attcgctgcc tgttgtcatg tccattagtt ctaccattag
     1141 ttagtgggga taatgatctt tatcggttca ttgccaaaag taaatttgag gaattattaa
     1201 atcgtaaagc aaactctaat tatgctaaag aaggcagcga gatagcagaa agattatcag
     1261 aagcatatat tactaaagta ttccccagcc atgtgaagat acccctccaa ccgatagatg
     1321 agttgttgcc atatctttat atacattcta atgaagatga aaataaacaa catacaagct
     1381 attctgaatt tatcaaactt gtacaacaaa aattctactt tctttgtaat gggcaagaac
     1441 gaagcacaaa ttggccgcag ccgagaagcg cacgtgaagt tacgcaacta atccgttctt
     1501 tacctccgtc tactcttagt aaggaagatg attcgggaac tgatttatgg caacgcttcg
     1561 ctgtctgggc ggaagaacgt cgcgatggat tagcattaac caatgttgaa tcttatctgt
     1621 ttattaagaa tgcgaaagca gtagaagatt taaatctgtc aaatcttatt gcttttaatc
     1681 ctttactgca aaaaggaaaa tatccctggg cagaaaagga tttttataaa cagcagtccc
     1741 aacgtcggaa agagctcaat gcccccgaaa caaattcagg tatccttaat accgtatttt
     1801 ccgaacaaag gaaagatttt attttaagaa gtatgcctgc gctggaactc attatggagc
     1861 ctatgtatgt cactaagacg gtagcagaaa aaaatgataa ttctgcgctt atagcgatct
     1921 atacccattc tgattattac agccagcagc agaacagacg atgtcatata ttttttggca
     1981 gagcttttga aataatgttc tggtcagtat tagcgaaaac tgaaaatctt ccacaagaat
     2041 tttatgaaaa agataagttt aaatctttat ttggtaatat tttcaaaaaa gtaccattct
     2101 actcaatatt ttcaatgaac cctacaaagg ttgttgatga agaaaatgac gatggcagtg
     2161 aacctgattt ttcgcaaaaa ctggacgata gcattaatga actggtggaa gatatatata
     2221 tctgggcaac cagtaataaa ttgcgagcct tcaaaaataa aaatttaata cccttaatga
     2281 cgtgcgtttt taataaggta ttttcacaga tcaatgtact gagaaaaaac gtgcaggaca
     2341 gagttaaatt tagagatgaa catttgtcag atctggctaa gcgatttgag tatatgttta
     2401 ttaatgctat ctttactttc atcagagaag gggtagttgt caataccaat gtggcaacag
     2461 gcgcagctcc tgccagagta cgtaatttat cagagtttaa taggtatgat aaaacattat
     2521 ccaggaatat gtccgggatt ttatccgtga aagaggataa tggcttaacg atagtcaaag
     2581 agagtgaggg cgatatcgca gatctgttat ttgaaatttg gcatagccca ttatttaaat
     2641 taacaaccag gacatgttac ccaataggta aaataaattc gcaaaatacg gcccaggaaa
     2701 atttatcatc agattttaat tcattttttg aaaatggtat caacttcgaa ttgataaaac
     2761 aatattattg gcaaacttca aatcatgata atatcaggac agcagacgtt agggaatggg
     2821 caacttcacg tcttaatgaa gcaatcatcc ttttttcatg gatgaaagaa agcaagtcta
     2881 ttaaagcgaa aattgacgga cagagctacg agggtcggct ctttcgcggg cttcagcagg
     2941 cgctggaagg ttatgaggag gtctgagtat gtttaatcag gatccttatt ggctcattcc
     3001 taccctttgt ctggcatcag accgaatttt ttatgcacaa ttgcgagacc acttaggcca
     3061 gaaaagtagc ggtgaacgca aaaaagaaaa aaatggatat atactggtac aggcggcaca
     3121 agactatcaa ttctattttg gcggccgtat tcggaaagag gatgtgcaaa ataatgcctt
     3181 aatgtggcag atagaaactg gtaatgaaaa ttgcttatcg atgcttgata gtttgtcagc
     3241 atatttcctc acatggcgcg gcaattgttt tgaggtcagg cgtgagcgac ttgaaccctg
     3301 gctgatgatc tgttccgtga tagatcccgc atggattatt gcctatgcat accaacaatt
     3361 gattaaacaa aatgttgtat gtgatagtga gcttatttct ttgctgacag aacatcaatg
     3421 tccatttgcc tttccaaaag gcagagggga catttccttt gctgataatc atgtccatct
     3481 taatggtcat ggttatagtt caatttcaat gctgaacttt atagatggaa attataaggt
     3541 taaaaaaggg ataaaatggc cctatcggca ggaatacacc ctctttgaaa gtggtcttct
     3601 ggataaaaat gatcttcccc gctggctgtc cgcttatagc tcttgcttac ttaaaaatgt
     3661 atataattca tttcaacaag gaaaaagatc cgaggtagat ttcacatgtc tgaaggatgc
     3721 ggtcgaaacg gtgcttgcgg atgaggataa atattatttt ttagaggtag cttcgctata
     3781 tgatgttgtc accttgcagc aaagagtgct ttatgaagcc gcccagcaga aatatcactc
     3841 acatcaacgt tggttactgt atacttgcgg aataatgtta ggtacagaat ctgaagatta
     3901 tgcgaatgcg ctggctaacc tgatccgaat cagcaatatt ctaagaaact atatggttgt
     3961 atctgcggtt ggattgggac aatttattga ttttttcggc ttcaactatc gtcgaataac
     4021 aaagccagct gatacaaaca accgagttca ttatgattct tctgctggta tttccagaga
     4081 atatcgtgtc tctcctgatt ttgtactggg tagcggcgta atgcctgata tatatgccag
     4141 gcaacttttc gatttttatt gtacccaagc acgcaagggc gtacccgaac aaggacatat
     4201 tgttgttcat tttacacgtt cctttcctga caaaaaatca acatatgata aattgctaac
     4261 cgagtgtcgc gaacggttac gttctcagtg tgattatttt ggccgttttt taacatcgct
     4321 tactttgcag tcgatagaat ataaaaattt atctactgat gaagatcgaa gcatagacat
     4381 tagaaaatta gttcgtggct atgatgttgc tggaaatgaa aacgagctac aaatagaggt
     4441 atttgccccg gttctccggg tactgcgtgc tgctaaattt aaaggggagg gggtgaactt
     4501 taaaaggcta cagcgccctt ttattactgt acatgctggt gaggattatt gtcatatact
     4561 cagtggcctt cgggctatgg atgaagccgt tgaattttgt atgttaggag aaggcgatcg
     4621 tatagggcat ggattagctc tgggagtaga tataaaacta tgggcgaatc gccaaaagcg
     4681 agcatacctg acggttggac aacatcttga taatttggtt tgggcatatc atcaggcagt
     4741 attactttct caacatattg tcgagcatat accagtaatg catgaattaa gggataagat
     4801 ccattattgg tctcatcaat tatatagtga aacttatacg ccagatttac tctttaaagc
     4861 atggctgctc cgccgtaact ggccggatta taagtcaatc atatctgatc cagcaaatat
     4921 caatgaatgg gtgcctgacc aacatatttt agtcagtaca gatgagacta cagctaaggc
     4981 cagaaaaatt tgggaacgtt atttaaatag cggtctggca gaaaatgatg tttttaacag
     5041 aataatttca gtaaattgtg cgcccgatac agcgcaaaat ttttcaatga cctttaatga
     5101 aaatgaagat attttatcca aaggggaatt attattgtat gaagctatcc aggatttctt
     5161 aatcgaaaaa tatagtaggt tgggtttagt catagaagct tgtccaacct caaatattta
     5221 tattggcaga ctggagaaat atcatgagca cccattattc cgttggaatc ctcctgactc
     5281 ccaatggatt aaacctggtg ggaaatttaa tcgctttgga ttgcgcacag gacctttatc
     5341 tgtctgtata aatacagatg acagtgcatt gatgccaacc acaattgaaa acgaacatcg
     5401 cttaatgaga gactgcgcca tacattttta tggtattgga acatggatgg cggatttatg
     5461 gataaactca atacgcataa aaggtattga aatattcaaa ggtaatcatt taagtcagga
     5521 tttagataat ttaatctaaa tgtaaacaag aaatccacgc aaatgcgtgg attttaagtc
     5581 aacttattat tctctgaaac ggtttaaccg ttcggaacaa cagattaaat ccgcaataac
     5641 tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaacctcagg
     5701 catttgagaa gcacacggtc acactgcttc cggtagtcaa taaaccggta aaccagcaat
     5761 agacataagc ggctatttaa cgaccctgcc ctgaaccgac gaccgggtcg aatttgcttt
     5821 cgaatttctg ccattcatcc gcttattatc acttattcag gcgtagcacc aggcgtttaa
     5881 gggcaccaat aactgcctta aaaaaattac gccccgccct gccactcatc gcaatactgt
     5941 tgtaattcat ttaacattct gccgacatgg aagccatcac agacggcatg atgaacctga
     6001 atcgccagcg gcatcagcac cttgtcgcct tgcgtataat atttgcccat agtgaaaacg
     6061 ggggcgaaga agttgtccat attggccacg tttaaatcaa aactggtgaa actcacccag
     6121 ggattggctg agacgaaaaa catattctca ataaaccctt tagggaaata ggccaggttt
     6181 tcaccgtaac acgccacatc ttgcgaatat atgtgtagaa actgccggaa atcgtcgtgg
     6241 tattcactcc agagcgatga aaacgtttca gtttgctcat ggaaaacggt gtaacaaggg
     6301 tgaacactat cccatatcac cagctcaccg tctttcatcg ccatacggaa ctctggatga
     6361 gcattcatca ggcgggcaag aatgtgaata aaggccggat aaaacttgtg cttatttttc
     6421 tttacggtct ttaaaaaggc cgtaatatcc agctgaacgg tctggttata ggtacattga
     6481 gcaactgact gaaatgcctc aaaatgttct ttacgatgcc attgggatat atcaacggtg
     6541 gtatatccag tgattttttt ctccatttta gcttccttag ctcctgaaaa tctcgataac
     6601 tcaaaaaata cgcccggtag tgatcttatt tcattatggt gaaagttgga acctcttacg
     6661 tgccgatcaa cgtctcattt tcgccaaaag ttggcccagg gcttcccggt atcaacaggg
     6721 acaccaggat ttatttattc tgcgaagtga tcttccgtca caggtattta ttcggcgcaa
     6781 agtgcgtcgg gtgatgctgc caacttactg atttagtgta tgatggtgtt tttgaggtgc
     6841 tccagtggct tctgtttcta tcagctgtcc ctcctgttca gctactgacg gggtggtgcg
     6901 taacggcaaa agcaccgccg gacatcagcg ctagcggagt gtatactggc ttactatgtt
     6961 ggcactgatg agggtgtcag tgaagtgctt catgtggcag gagaaaaaag gctgcaccgg
     7021 tgcgtcagca gaatatgtga tacaggatat attccgcttc ctcgctcact gactcgctac
     7081 gctcggtcgt tcgactgcgg cgagcggaaa tggcttacga acggggcgga gatttcctgg
     7141 aagatgccag gaagatactt aacagggaag tgagagggcc gcggcaaagc cgtttttcca
     7201 taggctccgc ccccctgaca agcatcacga aatctgacgc tcaaatcagt ggtggcgaaa
     7261 cccgacagga ctataaagat accaggcgtt tccccctggc ggctccctcg tgcgctctcc
     7321 tgttcctgcc tttcggttta ccggtgtcat tccgctgtta tggccgcgtt tgtctcattc
     7381 cacgcctgac actcagttcc gggtaggcag ttcgctccaa gctggactgt atgcacgaac
     7441 cccccgttca gtccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg
     7501 aaagacatgc aaaagcacca ctggcagcag ccactggtaa ttgatttaga ggagttagtc
     7561 ttgaagtcat gcgccggtta aggctaaact gaaaggacaa gttttggtga ctgcgctcct
     7621 ccaagccagt tacctcggtt caaagagttg gtagctcaga gaaccttcga aaaaccgccc
     7681 tgcaaggcgg ttttttcgtt ttcagagcaa gagattacgc gcagaccaaa acgatctcaa
     7741 gaagatcatc ttattaatca gataaaatat ttctagattt cagtgcaatt tatctcttca
     7801 aatgtagcac ctgaagtcag ccccatacga tataagttgt aattctcatg ttagtcatgc
//
