LOCUS       sequence-304765-        9023 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses the RADAR defense system.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9023)
  AUTHORS   Gao L, Altae-Tran H, Bohning F, Makarova KS, Segel M, Schmid-Burgk 
            JL, Koob J, Wolf YI, Koonin EV, Zhang F
  TITLE     Diverse enzymatic activities mediate antiviral immunity in 
            prokaryotes.
  JOURNAL   Science. 2020 Aug 28;369(6507):1077-1084. doi: 
            10.1126/science.aba0372.
  PUBMED    32855333
REFERENCE   2  (bases 1 to 9023)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1126/science"; 
            journalName: "Science"; date: "2020-08-28- 28"; volume: "369"; 
            issue: "6507"; pages: "1077-1084"
COMMENT     Sequence Label: pLG015 (RADAR)
FEATURES             Location/Qualifiers
     source          1..9023
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      6803..6850
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     CDS             complement(7070..7729)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     7644..7663
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(7730..7832)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     primer_bind     complement(8224..8241)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8358..8903)
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
     primer_bind     complement(8438..8457)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgagatc
       61 ccggtgccta atgagtgagc taacttacat taattgcgtt gcgcatgtgg ttagttatca
      121 cagcactaac ctattttcga gctttttgat tgaccaatac catttctttt aattatgaat
      181 aatgatgcgt caaccgatgg cgaacgggcc aaatccactc ttctacaact gcccattgtc
      241 acggtgtgga ataattaaaa attttagatt tttgagatta ttctcattac catcttgatt
      301 ttatttggtt ttgcatcaaa attcatagtt cacaagcttt tctcactcca aaaacaactg
      361 taaagggatt attgtgaaca cgatatacat accattagac agcggagagt ctgcggttct
      421 taaggatcca gataccttac ttccccgaaa tatttacgaa cagcttactc gatttattga
      481 aaaggctgtt aatgaagtac cgaagcctca cgaagcgctt aatgaaaccc gtagccataa
      541 ggctatatcg attgacggcg caagggggac aggaaaaacg tcggtgctag tgaatttgaa
      601 cgactatctg cagagtaatg ctcagcaact ggcggggaaa attcatatcc ttgatcctat
      661 cgatccgact ctacttgaag atggtgagtc gctgttcttg catattattg ttgctgccgt
      721 gcttcatgat aaagagatca aaactgccca aagcagagac ctcgataagt ccagagtgtt
      781 tacccagaag cttgagaact tggcacacgg actggagtcc gttgatttgc aacagaatca
      841 acgtggaatg gataaaattc gctccttata tggcagcaag catctggcaa attgcgttga
      901 agagttttta aaatctgcgt tggagttgat cggaaagaaa ttattgatac taccgattga
      961 tgatgtggac acttcactaa accgggcatt tgaaaatctg gaaatattgc gtcgttatct
     1021 tacctctccg tatgttttgc cggtagtgag cggcgatcgc cgtttatatg atgaggtctg
     1081 ctggcgagat tttcatggaa ggttgaataa ggattcagca tataatcgca agaacacata
     1141 tgatattgct agagatttgg caattgagta tcagcgtaaa attctgccgc taccgcgcag
     1201 actgagtatg cccgatgtaa gtgattactg gcagcaagat ggtatcgaag ttacgctaga
     1261 taaaaatggc attcctctgc gtaattttat ggcatggttg aaaatattta ttactggccc
     1321 cgtgaatggc cttgagggta gtgatttacc tctaccgata ccttcaatac gtgctttaac
     1381 ccagttcatc aaccattgca gggatttaat tcgtgagctt cctgaaccat tcagaaagaa
     1441 agtcagtacg ctggccttac gtcgtatgtg gcaaatgcct gatgttcctc ttgatgttct
     1501 tgaaagtttt gctgaaaaac atcgggaatt gagtaaagaa gctaagcgtg aatatgggga
     1561 ggcttacaag ctattttatg atggactaaa gaattttact gcttgggata gtaaggctta
     1621 tctagaagat gataaacaat ctgcatggct cgataggttg tgtgagtatt ttcgttttga
     1681 acctaaggct ggggctgtgt ttttaacgct tcaggcaaaa cagttctggg tctcatgggc
     1741 gcagggtgac aatcgtaatc aatcgattct tgcgactccg ctttttcaac ccttattgca
     1801 taattttcgt gaatacgatg tctttgaaag gtatgatgat ctttctgatt gggaatctca
     1861 gttaagaaca aggttaccgg agagttggtt gactgccatt aaagggcaaa aaacgctttt
     1921 accctatcct gtagcagaag cgggaattaa taccagttta aagtggaggt attgggaaga
     1981 attagagaac tatgggtttg atcctgcttt ggaaagcaag gcaaatttcc ttttgtccac
     2041 gttgatgcag aggaattttt atacaaactc taaacagtca gtcgtgataa atattggtag
     2101 agtttttgaa ataattattg ctagtcttgt ttcggattta gagttggccg acttgcagag
     2161 aattagacaa cgttctccat tttactctgc tagcgcgctt gcacctacca aaacgttaga
     2221 tttggaagag gattttacga aaaagaatac aagatttatg aataacagaa gtgaaactga
     2281 cagagacatt tctgatgata ttcttgttga tgtgccggat aaaaatgagg acgcatggaa
     2341 aaaaatttgt gatgaaataa accattggag aaagacacac aatgtggcta gtacaaactt
     2401 atcaccttgg ctggtttata aggtctttaa taaaacatat agtcaggttg ctaataatgt
     2461 gtttgttccc agtggaatgc aaaatgttga tgcggctcta aatgtttttg gtagggtttt
     2521 ttatgcagtt tggtcagcat ttggtagttt tgaaaaaggc gaattgttcg gactatccga
     2581 tgtggttgct acaactaata ttatttcggc aaaaaatttt tataatcatg ataacttccg
     2641 agtgaatgtt ggaccgttta cgcctgagca aaaccaaaat tctgacagcg atcgtgaggc
     2701 atatcagcat cgcaaaatgt atggtgaaaa aaccagagcg gtaagttatg tattagcaac
     2761 tcatccgctg aaaaaatgga tcgacgaggt attacgcact gagtttaaac aaaaacagaa
     2821 tgctcagatt cagaccgaga gaaaaatgcc gattcaggct gagaaaatta tagatatcag
     2881 cccggcaaga gagtttatca caagaaaact ttcattaaat tcacactccc ggttggttaa
     2941 aacacgtata ataaaacagc ttaagatgtt atatccaaac tacgataagg ctaaggactt
     3001 cattgatgaa gttacaaacc acttccctca gaatgatccc gcaattaata cgcttcagaa
     3061 agcatttgca gaactttacc ccgatggtga caaataatgt taactcggtc tctaagtgaa
     3121 catgctgcag ggtgtttttt cactgatgag cgtctgtcac aacgctttct agatatcctt
     3181 ttatcgccac ccaaggattt tgaaacgtgg tcatcattgc aggaggaatc tttcaagctg
     3241 ctcgttaaga gcatcgatag ccgatatcca cgcacttacc ggttaaccga cgtacgccag
     3301 cttgtgggga acatatgtga caacgggtta ctgacgagtc cgacactacc ttggctcgat
     3361 gtcattgcgg atcagttact gttgcggaat ggcgacttac tctattaccg cgaaaataag
     3421 gttcaagact acgtgcgaat agctgcggaa ctcgaccctg cccttctagt gggatggcgt
     3481 cttggcgact ggcttttgca aagcccaccg ccgcgattga cggacataac ccgtgtggtg
     3541 atggcgcaga atccgttttt tgctccacct gctaatgcag gtaaaccttt tgccgagggg
     3601 cacgtacatc tcgggggagt gacggctgga gatactattt tggatggcta tctttttgaa
     3661 gagattgaac tacccaaaag caaagatatg ttgttgtggg cgcacaaaga gcatgatgag
     3721 ttaacaccgt tgataaatcg agcaaagtct ttgcttacag ttctactttc tgccccccct
     3781 caaacggttt ctgagcaaac tcaaaatggt tttgatcagc gtaaaactgt atctgagaag
     3841 tacaaggcat tacagaaccc aatggatagc atccatcgtc tcccagactg gttattgctt
     3901 gctaaaaaga atcgcggaac tgaaagcgtc agccccggct ggtttttaaa ccaactggcg
     3961 catgcctccg aaaaaaaaca tccctcgcgc tggctgtggc tgcagctata cctttgccac
     4021 tcttatcagc ttaaagacac tcatccactg gagcgcacgg caatactctg tttttggctt
     4081 acggtaaatg cgctacggcg tcacattatt atggacggac aggggcttgc gtgttttacc
     4141 gagcgttatt ttaatggtgc tttacgtgcg ggtaagaaag ctgacagtag caatatgcgc
     4201 tacctgtttg ccggtaaaga cgatgtggcc gaagtgaaag catccccaaa ggctttcgat
     4261 catgagatgg tcactggatt ttcctcgaca ttgctgaaaa ccctcggcat tccagctgtt
     4321 tttccaccgt atatttttgg tgagcatgag attaagccag atgaacgcgt gctgcgctat
     4381 attggagcac tggagcgctg gcagttttgt gggcactttt ctcgctctaa aactgcaagt
     4441 cgcggcaagc gagcaaaggc tgatttgcag gctaactgga cagaagcgga gcgattgtta
     4501 cagaaactgt acagtcataa tggctggaat catcccgtct tcttaggggg taaacgtaac
     4561 ccacattttc attttcagcc gtcgaactgg tttcgggggc ttgatgttgc aggggatgaa
     4621 aacgtactaa aaattgcagg ctttgccccg atgctgcgct ggctacgaag tggattatat
     4681 cccgtaccag aagggcttcg cgccagtatg agttttcatt tcagtattca tgccggggag
     4741 gattacgcac atccggcgtc aggattgcgt catattgatg aaacggttcg cttctgcgaa
     4801 atgcgggagg gagaccggct aggacatgct ctggctctcg gaattgaacc tgcgctctgg
     4861 gcgaaacggc atggtgaaat gatactacct ctggatgaac atttagataa tcttgtctgg
     4921 cagtggcact atgctacgct tttatcggct tcattgcctc tcgctcaggc ggtattaccg
     4981 ctgcttgagc gtagaattgc acgctttatt gcacggtgcg aatggtgcaa aaagagacct
     5041 ccgcaaatag ataacagtgt ggtggggaaa caggcctgta gtgatgataa acctctggaa
     5101 aatattacac ctgatacgct ctaccgggcc tggctactgc ggcgtaattg ttcatatcga
     5161 ctccagcaac tccacggcgg ttcccctttg acctcgcaag agaaatgtgc gctgccggat
     5221 tgggccacgc tcagcgataa aggcaatgtg gcggcgcagc tttatcagca aagacactcg
     5281 agtctccttg acgatatgcc gccgcaactg gtagttgtgc gtgtagcgga cgaatgggga
     5341 actcaggagc ttattggctt gggaaatcct ggtaaactgc gtcagcaggc tcttgacggt
     5401 aaagatatcc tccaagacat tgatacgccg gtagagctgc aatttatgca tgctttacag
     5461 gactatttgc tagatcacta tgatcgtaaa gggttaatta tagaaaccaa cccaacatca
     5521 aacgtatata tcgcgcgatt caaaaagcac gtagagcatc ctatttttcg ttggaatcct
     5581 ccggatgaag aactgttgaa accaggcgct gaatttaatc gttatggatt gcgccgtggg
     5641 ccagtcaggg ttctggtcaa tactgacgat ccagggatta tgcctacgac attacggacg
     5701 gaatttttac tactgcgaga ggctgcgatt gagcgtggtg tcagccgaac gatggcagaa
     5761 tattggctgg aaaggctgcg cctgtacggg ctggaacagt ttcagcgtaa tcatttaaat
     5821 gtatttgaag ttattgaata gaggatttta tcgtgagtgg tacattccct tacttgcaat
     5881 atacggatgt caatgggcta caacctaagc tcaaagaaga gttgaaaaat ttacggagaa
     5941 aagagtattt gtcctactgg cctcgttttc tgatacgtag aatttcgctt tatgctcttc
     6001 cattcctcat gttcttcact tttttctttt gtctgagtct gacgaagaaa gttggggcag
     6061 aggaagtgac taatattctt ggaaccgtga gtatatcctt cagtagttgc ctgctgctgg
     6121 ggattattat ttctggtgtc gtgttactct tgcagtggac gtgcttcaac tgtaaataca
     6181 gtccgcagga tacgaatgga gttgttgggg ctcgtaagtt aaattataaa ttacttgctc
     6241 atgttgtatt tgttattgca tgcgtgcttt tatttgtttt tatttattgc accaataata
     6301 aagtgtttta tggttttatc gtgtttcttg gtttgacatt attaccattg gtaattgacc
     6361 gtaccttggg ggtgactcgt caaaatgaac gtcacaaact ctatatcaga aggttagagc
     6421 gcctcgatga attgaatatt ctccgggaga aaatgaatat taaattcgaa gaatcccatt
     6481 tcatcgagta tatgaagctt gttgatgaag ctgatcacgg aaaaaaccag gatacagtaa
     6541 gcgatacatc ctattttatg acgttgatag aaaataagct aaaagtgtaa tcggttttaa
     6601 tatgatgctg tataaaaaac tacgcaattg cgtggttttt tgtcggacta tgagggcaag
     6661 gttgccctaa aacagaggtt aaacgttggg atgtgattta ttgcacatca tgccgtgccc
     6721 atccagtaga atccggttcg aaatgtgtat aggattgtgt atatgtttct gttcggtctc
     6781 ggattcttat acaccgcaat aactagcata accccttggg gcctctaaac gggtcttgag
     6841 gggttttttg ctgaaacctc aggcatttga gaagcacacg gtcacactgc ttccggtagt
     6901 caataaaccg gtaaaccagc aatagacata agcggctatt taacgaccct gccctgaacc
     6961 gacgaccggg tcgaatttgc tttcgaattt ctgccattca tccgcttatt atcacttatt
     7021 caggcgtagc accaggcgtt taagggcacc aataactgcc ttaaaaaaat tacgccccgc
     7081 cctgccactc atcgcaatac tgttgtaatt catttaacat tctgccgaca tggaagccat
     7141 cacagacggc atgatgaacc tgaatcgcca gcggcatcag caccttgtcg ccttgcgtat
     7201 aatatttgcc catagtgaaa acgggggcga agaagttgtc catattggcc acgtttaaat
     7261 caaaactggt gaaactcacc cagggattgg ctgagacgaa aaacatattc tcaataaacc
     7321 ctttagggaa ataggccagg ttttcaccgt aacacgccac atcttgcgaa tatatgtgta
     7381 gaaactgccg gaaatcgtcg tggtattcac tccagagcga tgaaaacgtt tcagtttgct
     7441 catggaaaac ggtgtaacaa gggtgaacac tatcccatat caccagctca ccgtctttca
     7501 tcgccatacg gaactctgga tgagcattca tcaggcgggc aagaatgtga ataaaggccg
     7561 gataaaactt gtgcttattt ttctttacgg tctttaaaaa ggccgtaata tccagctgaa
     7621 cggtctggtt ataggtacat tgagcaactg actgaaatgc ctcaaaatgt tctttacgat
     7681 gccattggga tatatcaacg gtggtatatc cagtgatttt tttctccatt ttagcttcct
     7741 tagctcctga aaatctcgat aactcaaaaa atacgcccgg tagtgatctt atttcattat
     7801 ggtgaaagtt ggaacctctt acgtgccgat caacgtctca ttttcgccaa aagttggccc
     7861 agggcttccc ggtatcaaca gggacaccag gatttattta ttctgcgaag tgatcttccg
     7921 tcacaggtat ttattcggcg caaagtgcgt cgggtgatgc tgccaactta ctgatttagt
     7981 gtatgatggt gtttttgagg tgctccagtg gcttctgttt ctatcagctg tccctcctgt
     8041 tcagctactg acggggtggt gcgtaacggc aaaagcaccg ccggacatca gcgctagcgg
     8101 agtgtatact ggcttactat gttggcactg atgagggtgt cagtgaagtg cttcatgtgg
     8161 caggagaaaa aaggctgcac cggtgcgtca gcagaatatg tgatacagga tatattccgc
     8221 ttcctcgctc actgactcgc tacgctcggt cgttcgactg cggcgagcgg aaatggctta
     8281 cgaacggggc ggagatttcc tggaagatgc caggaagata cttaacaggg aagtgagagg
     8341 gccgcggcaa agccgttttt ccataggctc cgcccccctg acaagcatca cgaaatctga
     8401 cgctcaaatc agtggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct
     8461 ggcggctccc tcgtgcgctc tcctgttcct gcctttcggt ttaccggtgt cattccgctg
     8521 ttatggccgc gtttgtctca ttccacgcct gacactcagt tccgggtagg cagttcgctc
     8581 caagctggac tgtatgcacg aaccccccgt tcagtccgac cgctgcgcct tatccggtaa
     8641 ctatcgtctt gagtccaacc cggaaagaca tgcaaaagca ccactggcag cagccactgg
     8701 taattgattt agaggagtta gtcttgaagt catgcgccgg ttaaggctaa actgaaagga
     8761 caagttttgg tgactgcgct cctccaagcc agttacctcg gttcaaagag ttggtagctc
     8821 agagaacctt cgaaaaaccg ccctgcaagg cggttttttc gttttcagag caagagatta
     8881 cgcgcagacc aaaacgatct caagaagatc atcttattaa tcagataaaa tatttctaga
     8941 tttcagtgca atttatctct tcaaatgtag cacctgaagt cagccccata cgatataagt
     9001 tgtaattctc atgttagtca tgc
//
