LOCUS       sequence-304767-        9684 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses the AVAST type 1 defense system.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9684)
  AUTHORS   Gao L, Altae-Tran H, Bohning F, Makarova KS, Segel M, Schmid-Burgk 
            JL, Koob J, Wolf YI, Koonin EV, Zhang F
  TITLE     Diverse enzymatic activities mediate antiviral immunity in 
            prokaryotes.
  JOURNAL   Science. 2020 Aug 28;369(6507):1077-1084. doi: 
            10.1126/science.aba0372.
  PUBMED    32855333
REFERENCE   2  (bases 1 to 9684)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1126/science"; 
            journalName: "Science"; date: "2020-08-28- 28"; volume: "369"; 
            issue: "6507"; pages: "1077-1084"
COMMENT     Sequence Label: pLG017 (AVAST type 1)
FEATURES             Location/Qualifiers
     source          1..9684
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        105..209
                     /gene="bla"
                     /label=AmpR promoter
     terminator      7464..7511
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     CDS             complement(7731..8390)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     8305..8324
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(8391..8493)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     primer_bind     complement(8885..8902)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9019..9564)
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
     primer_bind     complement(9099..9118)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgagatc
       61 ccggtgccta atgagtgagc taacttacat taattgcgtt gcgccgcgga acccctattt
      121 gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa
      181 tgcttcaata atattgaaaa aggaagagta tggtagcgat aaaaatgtat ccggcaaagg
      241 atggggatgc ttttcttatt atttgcgatg aggaaaaaag tgcatttctg attgacggag
      301 gctacgcgga aacgttcagg caacatattt tgcctgactt acgtgagctg agttttaacg
      361 gttaccggtt acgtctggtc atggcaacac atattgattc agatcacatt ggtggtctcg
      421 tggacttctt tcttgtaaat ggacacgcag cagagcctgc agtgattact gttgaccgcg
      481 tatggcacaa cagcctcagg gcgatgacga gacccgaaaa taatgcacaa aaagtggatt
      541 cccgagaaat cactgacttt ttgagacgga gatatcatgt cgaagccgat aaagccaaac
      601 cgcatgaaat cagcgcgcgt caggggagtt cactggctgc cagccttctg gctggcgatt
      661 atcattggaa tgagggaaaa gggtatcagt gtatctgcac cggtacctcc attcccaact
      721 tgatgtgcga taacagtcta acaattctga gcccctctaa ggagagaatt tcagcgctct
      781 gcctgtggtg gcgcagacaa cttgcatcgc tgggcttttc gggacggtcc tcctcgagtg
      841 aggcatttga tgatgctttc gaattttttt gtaaaaggga agcatctcag gttcctcttc
      901 cgcatgtcat caatgcaaga acaccgttgc ttgagaggga ttatgcacgg gatacctcgc
      961 caacaaatgg cagttcgata gcgttcagtc tggtgctcaa taagaagaga atattgatgc
     1021 taggagatgc ctgggcggaa gaagttgtga catctctggg tgccagtggg gcgtcccatc
     1081 attttgatat cattaaaatc tcacatcacg gtagtattag aaacacaagc ccgaatcttt
     1141 taaagatcat agatgctcct gtgtacctga tctcaaccga cggaaaaaag catgccagac
     1201 accctaacct ggcggttctg aaagcgattg tggacagacc tgcggcgttt acgcgaacgc
     1261 tctattttaa ctatgccaac agcgcatctg cttttatgaa aaattacctt tctgcaagtg
     1321 gtgcacaatt cagaatcatt gaaggatcaa cggattggat aacactgtga gatatgctgc
     1381 tactgaaact gaaataagga acgcaactgt actcattgaa tgcgcgggtt acactggttc
     1441 cggaaccctg atcgcagcag acaaggtcct tacggctgca cattgtgtag tatcggatga
     1501 tcctgagaca ccaattacag tgacattttt tggtgcggat gaagacgtct gtgtcaatgc
     1561 gacaatttca gaaatagata catcgtgcga tgcctgtctg ctaacacttt ctgactctgt
     1621 cgacattccg cctattacac ttatgacaca gccggagcga gagggaagcc aatggaaagc
     1681 ctttggctat ccggcatcac gcaatgggcc atcacattat cttcatggca ctataagtca
     1741 gattttacca aggcttttcc atggcgttga tatggatttg tcggtcagtg ccgattgtgt
     1801 tctggaagag tacagtggag tttctggtgc cgccattcta tcagaaaata aatgcattgc
     1861 gatggtgcgc atcaggatgg atggtggact aggtgcagta agtcttgata agttaagcgg
     1921 tttgctgatt cgaaacggcc tcatcccaga tgacattgca tccctgccag attcatcact
     1981 gtcgggtgaa gttgtcctga accgcacaga atttcgcgac aactttgaat cgttcgtcct
     2041 ggagcacaag ggacgtgcag tgcttttgga aggtagtccc ggctctggta agactacctt
     2101 ctgccgccat tatcagcccc gtagtgagca actcgcagtg gcgggtgtct atgaatttac
     2161 accggaagac ggtgctggta cgacattcaa aattcttcct gaggtatttg ccgattggct
     2221 gcataaccag gtttctatac tgctttcagg taggcctgct cgcagggagg aaacagaaaa
     2281 gatcaatctg acccaaaagg tgtctgacct tctacatact ttctcagatt actggaagca
     2341 caaaggaaaa tatggcgtca ttttcattga tgctgtgaat gaggcaagcg agtgcgggga
     2401 tgaggcagta tcgcgcttta cagcattact gccggtgaca cttccggaga acgtcaaact
     2461 tgttttcacc gcaccatcat tatcatcagc tggtaaggct ttccggcact ggctcacacc
     2521 tcaggattgt atcagcctaa cgcttttaag ccatagggag gtgttacagc taacagctcg
     2581 agagcttaaa acttccgccc cttctttgtc actactcaca cgagttagtg atatagctca
     2641 gggccatcca ctttatctcc gatacattct tgggtatctg aaagcgaatc cggatcaggt
     2701 taatctggag atattcccgg ttttcagtgg cagcattgaa acctactacg aaaggctctg
     2761 gcaggggctg gttaaggatg agagcgctgt aaatctgctc ggtattctct cgcggatgcg
     2821 ctggggcatt gatatttcat cactgatccc tgttctaaca ccgcaggaac agacggtgtt
     2881 tgttccaacc cttgaccgta ttcagcatct gcttcttaat gataaatcat cagcattgtg
     2941 ccaccaatca tttgcggcgt ttatcaacag taaaacggcg gtaattaact cgctgctgca
     3001 cggacgcctt gccgacttct gccttaccag tggagagagt tatggcctga ttaatcgcgc
     3061 ttatcacctg ctcctagcct ctcacgacag acatcctgaa gccgcattgg tgtgcacgca
     3121 ggaatgggct gacgcctgta tcgtcaaggg ggctcagccg gatattctaa ttcacgatat
     3181 ccgtcagacc ctgaagaaca cgcttattcg tgccgatgca gtggcatcga ttcgtctgtt
     3241 gctgcttttc caacgcatga ccttcagaca ccattttttg tttctgcagt cagcttatca
     3301 ctcaggcctt gccctggctg cacttggcag accggatgag gcccttgagc agctcatacc
     3361 atctggaagc ctcgttgttg atgcagttga tgcaattgtc agcgcacaga ctctcgcgcg
     3421 tatgggaaac agtgaacacg cgctgaagct attggaaaag gtgaagtcag ctgtcgacca
     3481 agaatttgaa cgcaatcccg tcaatctatc tgattttatc ggcctttccc tggcttgggt
     3541 gagagctgag ctgatggctg gggtggttga tggccacgga cgcacacgcg aggttgttga
     3601 gtatttgtac ggttgtgggc aagtcgttcg cgataatttt gaacaatcag cgcatagtaa
     3661 atcagcatat acacgcgctt tttatcctct tcaggcagaa atggaagccg tgaacatagc
     3721 ctttaatgac cgctccgtat ctttacggac ggttaaagaa aagtttggta gcttaccgga
     3781 aaatattctt gatctgatgc tcagttcagt tatgcgggca catgacatca ttctgcaaca
     3841 tcagttgccg atgccccagc atgctttgca acccgtttgg tacaatctgg acagattact
     3901 tcatactgat attccgtatt cgaacgaaat tcgttttaat tcattaagta gccttatttt
     3961 tttcaatgcg ccttctgctc ttattatcag gatggcgggg gtattttctt tcgaagtagt
     4021 acccgaaata acgttgctca atgaagaaaa tgagatagca gcagacagca ttgacgttag
     4081 tgaacaggga caactctggc tggtgagcgc ctaccttaat gaaacgcaac cctgtcccga
     4141 tattaaacat ccgagtcagg gatgttctga atggctcaag acattgactg aggctatttt
     4201 ttggtacagc gggcaggcgc gccgggcagt tattgacggc aacgatgaga aaaaagaact
     4261 gcttttagtc aaggtgcaga atgatattct ccctgctctt tcgtactcgc tggaagagcg
     4321 catggcatgg ccgaattcat gggcaatgcc tgaacagatt atccccatga tttacgaaga
     4381 gttagtaaac atgttcggcg catgctggcc cgataagata tcagtgatca ctgatttcat
     4441 tctggctcat acgcctcagc aatgtggact ttattccgag gggtacaggc gtttactgaa
     4501 cagagttatt cagactcttc taaatgagca tcggtttttg gggcaatctg atacgacatt
     4561 tcaactactt gagacgttgc atgcgtttgt ttctgctttt actgagaatc ggcaggagct
     4621 ggttcctgaa ttactgaata ttattccagc ttatattagc cttgatgctc ctcagctggc
     4681 acaggacact tacactgagc ttttaggtgt gtcgatgggc cctgactggt acaaagaaga
     4741 ccaatttgcc ctcatgacaa ctatgctgcg cgtgatacca cagcatacag acacaaatac
     4801 tacactttca caagttgcag gattccttga acatgcttcg ggtgaaatga catttaggcg
     4861 ttatgttagg caggaaaaat cacagtttat tggcgaactt attcgtcgtg ggaattatgc
     4921 acacgggttt aactattatc gtcagcagtc ctgcggatcc catgaggaaa tgctcaccca
     4981 acttagccac ccagctgcag atagccctca tccattgaaa ggcatgcggt tcccgggggg
     5041 agcgctggat gaggaacatg ctgtagaatg cattgtcagt gaactgcgaa acagagtcga
     5101 ctggcggctt cgctggggac ttcttgaaat attcagcttt ggcagtattg gtaatcttgc
     5161 agtgcccttt gctgaactta tcaatgaatt ttctgcagac actgaagacc ttaatgaaat
     5221 acccaaaagg ttgcacaaca ttttacatgg tgatgtgcct ttctcagaac acagaaattt
     5281 tatcaaaaat ttcacagagc accttgcaga caaccataag ccactctttg ctgaatttat
     5341 cagtttgcta tccgaagaca ctagcgataa cgacgttaag cctcccccct ctggtgatgc
     5401 taaccagaag ggtactgata cctcagatga tgtggcaatg cagccaggac tttttgggaa
     5461 gcgttctgcg atcaataggg ctgaagcctg catggaaaat gcccgaaaag ccgcagcacg
     5521 cagaaacaca gttcgtgcaa gtgagttagc cgttgaaagc ctgcatataa ttcaggatgg
     5581 tgactggtca gtctggagaa agaacaacca tctggcggaa cttacacgga cgtacatatt
     5641 ggacaactct gcggatgcag gttcggtcat tcgtgcttat gcttcgcttg tagaaaaaga
     5701 acgttatgcc ccggcatggg taattgctag tcatctcatc gaaatagcag ccagtaaatt
     5761 ctctgatcaa gaagcccaag ctattaacca gatcgtactt gaacacaacc gccacatgct
     5821 tgggaatacc gaagcggatg ctgcgcattt ttcttttctt aatgaacctg atacctcaga
     5881 tgcaggtgaa gaaacactct attttctgtt ttggctgctg gaacacccac tgaaattcag
     5941 acgcgaacgg gctctggaag tactgaagtg gcttgcatca gacgatgata agattctggg
     6001 ccaatgcgtg acggaggcac tcgtttcaga cattgcctca cgagctgaag cactaatggc
     6061 attgacagac tgggtgtcag ctagatctcc tcagcgaata tgggacttta tagttaaaga
     6121 gcgcagcctt tttgaatggc ttgaaggcac tactgcacta agccaagtcc atctcctgga
     6181 gcgagtaacc agcagagcgg gatttgtttt aagaaatgag attgccgcat ttgagcgacc
     6241 ccgaaagctt ttactgacat cagaagcctc tggacaacgg aatattccag aaaatttacc
     6301 aacatgggtg caatccttgt cgcagaccct tgccgtgatg gaaaagcagg gaatagatat
     6361 cccagctttg cttaccttac tcgaaaaacg ggttttacag cagagtggat tggctgatat
     6421 cacggtggct tttgagctgg aaaagttact tgcgcgtggt tttactgtga atagaacacc
     6481 aagtcaccat cgctgggaga cgatggtgcg atttgcatta aaccagatca tacatgaggc
     6541 ggccgcacag gatgaactgc aaaacattga acccttgcta cgtgcctgga accccgcgtc
     6601 agaggagtgt gttgagccgt gggaggtttg taaccgggca aaacagatta tctgcgctgt
     6661 tatggaaggt agacatcagc aagcttcggg catagaggat ggctttttct tgcattatct
     6721 tgatgaagtg gaggtttccc gagaaggtca aacgcatctg gtggaaatct cagcggtgtt
     6781 aacgacagct cataatggtc atgagagcct tagaccaggt gcagaaagcg aatttaatgc
     6841 aacacagaca cctgatatag agcggacgct tagtgtgcac cttacatgcc agcgagtcaa
     6901 aatgcagcct ttgctttttg ggggagctac gcctgccgca gtgtcgaaaa agtttatgca
     6961 gatgactgga acgttgcctt cagactttat tcgcaggcaa tggcgaagcg ggcgttctct
     7021 tagtaaaaac agatgggggg aaccaataag cagaggaagt ctgttactca tgaaaagaac
     7081 aactaccctc cctccaggac tgggcttagc gtggtatgtc actgtcgatg ggaagttgat
     7141 gaatatattt tcatatgccc cgaggaggag ataatgaaat acagttcaat ggaaacgcca
     7201 aaaacgcgag aggaatttga ggctcgctgt tttcacctgc tcaatgcgat caagttagga
     7261 cggtatcatg gcattccggg tgaaggtaac aaagagcagg ttccttttct ccctaacgga
     7321 cgagttgatc tggcaaacat tgataccatg actcgcctct cgatgaactc gttatatgat
     7381 ttccactata acagggataa ttatccgcag tttgatctct ctgaaaatga cgagaatgaa
     7441 gaggctacgg attgacgcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga
     7501 ggggtttttt gctgaaacct caggcatttg agaagcacac ggtcacactg cttccggtag
     7561 tcaataaacc ggtaaaccag caatagacat aagcggctat ttaacgaccc tgccctgaac
     7621 cgacgaccgg gtcgaatttg ctttcgaatt tctgccattc atccgcttat tatcacttat
     7681 tcaggcgtag caccaggcgt ttaagggcac caataactgc cttaaaaaaa ttacgccccg
     7741 ccctgccact catcgcaata ctgttgtaat tcatttaaca ttctgccgac atggaagcca
     7801 tcacagacgg catgatgaac ctgaatcgcc agcggcatca gcaccttgtc gccttgcgta
     7861 taatatttgc ccatagtgaa aacgggggcg aagaagttgt ccatattggc cacgtttaaa
     7921 tcaaaactgg tgaaactcac ccagggattg gctgagacga aaaacatatt ctcaataaac
     7981 cctttaggga aataggccag gttttcaccg taacacgcca catcttgcga atatatgtgt
     8041 agaaactgcc ggaaatcgtc gtggtattca ctccagagcg atgaaaacgt ttcagtttgc
     8101 tcatggaaaa cggtgtaaca agggtgaaca ctatcccata tcaccagctc accgtctttc
     8161 atcgccatac ggaactctgg atgagcattc atcaggcggg caagaatgtg aataaaggcc
     8221 ggataaaact tgtgcttatt tttctttacg gtctttaaaa aggccgtaat atccagctga
     8281 acggtctggt tataggtaca ttgagcaact gactgaaatg cctcaaaatg ttctttacga
     8341 tgccattggg atatatcaac ggtggtatat ccagtgattt ttttctccat tttagcttcc
     8401 ttagctcctg aaaatctcga taactcaaaa aatacgcccg gtagtgatct tatttcatta
     8461 tggtgaaagt tggaacctct tacgtgccga tcaacgtctc attttcgcca aaagttggcc
     8521 cagggcttcc cggtatcaac agggacacca ggatttattt attctgcgaa gtgatcttcc
     8581 gtcacaggta tttattcggc gcaaagtgcg tcgggtgatg ctgccaactt actgatttag
     8641 tgtatgatgg tgtttttgag gtgctccagt ggcttctgtt tctatcagct gtccctcctg
     8701 ttcagctact gacggggtgg tgcgtaacgg caaaagcacc gccggacatc agcgctagcg
     8761 gagtgtatac tggcttacta tgttggcact gatgagggtg tcagtgaagt gcttcatgtg
     8821 gcaggagaaa aaaggctgca ccggtgcgtc agcagaatat gtgatacagg atatattccg
     8881 cttcctcgct cactgactcg ctacgctcgg tcgttcgact gcggcgagcg gaaatggctt
     8941 acgaacgggg cggagatttc ctggaagatg ccaggaagat acttaacagg gaagtgagag
     9001 ggccgcggca aagccgtttt tccataggct ccgcccccct gacaagcatc acgaaatctg
     9061 acgctcaaat cagtggtggc gaaacccgac aggactataa agataccagg cgtttccccc
     9121 tggcggctcc ctcgtgcgct ctcctgttcc tgcctttcgg tttaccggtg tcattccgct
     9181 gttatggccg cgtttgtctc attccacgcc tgacactcag ttccgggtag gcagttcgct
     9241 ccaagctgga ctgtatgcac gaaccccccg ttcagtccga ccgctgcgcc ttatccggta
     9301 actatcgtct tgagtccaac ccggaaagac atgcaaaagc accactggca gcagccactg
     9361 gtaattgatt tagaggagtt agtcttgaag tcatgcgccg gttaaggcta aactgaaagg
     9421 acaagttttg gtgactgcgc tcctccaagc cagttacctc ggttcaaaga gttggtagct
     9481 cagagaacct tcgaaaaacc gccctgcaag gcggtttttt cgttttcaga gcaagagatt
     9541 acgcgcagac caaaacgatc tcaagaagat catcttatta atcagataaa atatttctag
     9601 atttcagtgc aatttatctc ttcaaatgta gcacctgaag tcagccccat acgatataag
     9661 ttgtaattct catgttagtc atgc
//
