LOCUS       sequence-304768-        7443 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses the AVAST type 2 defense system.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7443)
  AUTHORS   Gao L, Altae-Tran H, Bohning F, Makarova KS, Segel M, Schmid-Burgk 
            JL, Koob J, Wolf YI, Koonin EV, Zhang F
  TITLE     Diverse enzymatic activities mediate antiviral immunity in 
            prokaryotes.
  JOURNAL   Science. 2020 Aug 28;369(6507):1077-1084. doi: 
            10.1126/science.aba0372.
  PUBMED    32855333
REFERENCE   2  (bases 1 to 7443)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1126/science"; 
            journalName: "Science"; date: "2020-08-28- 28"; volume: "369"; 
            issue: "6507"; pages: "1077-1084"
COMMENT     Sequence Label: pLG018 (AVAST type 2)
FEATURES             Location/Qualifiers
     source          1..7443
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      5223..5270
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     CDS             complement(5490..6149)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     6064..6083
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(6150..6252)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     primer_bind     complement(6644..6661)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6778..7323)
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
     primer_bind     complement(6858..6877)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgagatc
       61 ccggtgccta atgagtgagc taacttacat taattgcgtt gcgcagggat ttccaccacc
      121 tcccaccgac catctaagac tttatgccac tgtccctagg actgctatgt actaggagcg
      181 gatgttaaac tcagactcgt ttcagctaca ttgcgttttg aataatattc catcataata
      241 actctttgaa aaatgtgatc ttttcattta taacactgat gacttgctta tctcattggg
      301 atatcggagg agaatactta actatgacaa gcccgattat tatgacactg gctatattat
      361 atagattgat attaaaatgt aggattaggt tcttgccaag gtgtcaagat ttacagatag
      421 gtttaaaacc atataaatat gttttacggt gagatacaat acatattgta aggcataaac
      481 gcttggtaaa attttaatta ttggaagaag ctaatcatgg aacccatatc aattacagtg
      541 gcaacttatg tagcaactaa acttattgat caattcatct ctcaagaagg atatggttgt
      601 attaagaaag cattattccc ccaaaaaaga tatgtggata gattatatca actaattgaa
      661 gagacggcaa ttgagtttga agaaacatat ccagtagaaa gtggagcaat accattttat
      721 cattccgaac cattgtttga gatgttgaat gagcacatct tttttaaaga gttccctgac
      781 aaagagatat tattagacaa gttcaaagaa tatccaagta tcactccccc aactcaacaa
      841 caactcagcc ttttttatga gatgttatca ttaaaaatca ataattgttc gaagttaaaa
      901 aagctacata tcgaagaaac gtataaagaa aaaatattcg atattaatga agagctcatt
      961 caagtcaaac ttattttacg gtctatagat gagaaactaa cttttcactt aagtgatgat
     1021 tggttaaatg aaaaaaatag tcaagcaata gctgacttgg gaggtcgata cacacccgaa
     1081 ctcaacgtaa agctagaaat agcagagata tttgatggcc tcggtagaac taatgatttt
     1141 tctaaaatat tttattcgca tatagatagc tttctggtcg ctggaaagaa attacatagt
     1201 tgcgatgtaa tttcctcaga attatttgaa ataaaccagt ccttaaaaga aatttctgat
     1261 atatatcagg agattaattt ttctaaatta gatgaaatcc ctataaataa atttaataac
     1321 tatgtttcta gctgccagac agctattggc ggagcggtat caatattgtg ggaactccga
     1381 gaaaagtcag agcaagtagg tgaaaccaag cattacagtg ataagtattc atctactctg
     1441 cgaatgcttc gggaatttga ctatgcgtgc aatgaattac gtatattcat taattcaaca
     1501 acagtgaagt tggctaacaa cccattctta cttctcgaag gaaaagcagg aattggtaag
     1561 tctcatttac tggctgatgt gattaaaaat cgaattgctt ctgggtatcc ttcactactc
     1621 atactagggc aacaacttac ttcagatgaa tctccatggt cacaaatctt caagagatta
     1681 cagcttaaaa tcacttctcg tgaattccta gaaaaactga atttatatgg caaaaaaaca
     1741 ggaaaaagag tcttagtttt tattgatgct attaatgaag gtaatggaaa taaattctgg
     1801 aatgacaata ttaacagttt tgtcgatgaa atcagatgct ttgaatggct tggtctgata
     1861 atgtcagtca gaacaacata tagaaatgta acaatttcac atgagaatgt tgtgcgaaat
     1921 aattttgaaa ttcatgaaca tattggattc cagaacgttg agttggaagc ggttagtcta
     1981 ttttatgatt attacaatat tgagaggcct tcatctccta accttaatcc agagtttaaa
     2041 aatcctctat ttcttaagtt attgtgtgaa ggcattaaga aaaatggttt aaccaaagtg
     2101 cctgttggat ttaatgggat ttcaaatatt tttaactttt tagttgaagg ggtaaataaa
     2161 tcattagcat cgccaaaaaa atatgcattc gatcccagtt ttcctcttgt taaagatgct
     2221 ctcaatgaaa tcataaaatt caaattagag attggtcgta atagtatttc acttaaagat
     2281 gctcactcag tggttcaatc tgtagttaat gattatgttg ctgataaaac cttcctcagc
     2341 gccttgattg acgaaggatt attgactaaa ggcatagtga gaaatgatga taattctact
     2401 gaggaagtag tttatgtggc ttttgaaagg tttgatgatc atttaactgt taatttttta
     2461 ttaaatgatg ttgaaaatat cgaaagtgaa tttaagcctg atggtcgtct gaaaaaatat
     2521 tttcatgatg aatgtgattt ttatataaaa tcgggaatag tagaggcgtt gtctattcaa
     2581 ttgccagaaa ggtatgaaaa agagctttat gaatttctgc cggagttcag caataatctt
     2641 aaattactag aagcctttat tgatagcttg atatggcgcg atattaaggc tattgatttc
     2701 gaaaaaatta gacctttcat caatgaacat gtttttaaat ttaaagatag ttttgatcat
     2761 ttcctcgagg cagtgatctc tatttcaggt ttagttggcc atccctttaa tgctaatttc
     2821 ttgcatgatt ggctaaaaga ttattctttg gcaaatcgag attcgttttg gactacagaa
     2881 cttaaatata aatatagtga agactcagca tttaggcatc taatcgattg ggcatgggcc
     2941 agaacagata aaagctttgt ttcggatgag tcaatcgagc tagttgcaac tagtttatgc
     3001 tggtttttaa cttctagtaa ccgagaactt cgagattgct caactaaggc tttagtgagt
     3061 ttactcgagc caagaattcc tgtattgaga aaaataattg ataagtttta tggtgtaaat
     3121 gatccttacg tttgggaaag aatatttgca gttgcattag gctgtacatt gcgaactgat
     3181 aatattaaag aactaaaata tttagccgaa actgtttacc aaaaggtatt ttgttctaag
     3241 tatgtgtatc caaatatatt acttagagat tatgctagag agattattga atttgctaat
     3301 catcttggat tggaacttga aagcattgaa ttatccaaga ctagaccacc ctacaacagc
     3361 atttggcctg acaagattcc ttcaaaagag gaactagagt ccctttatga taaagaacct
     3421 tatcgggaac tctggagctc tattatggaa gatggtgact tttcacgata tactattgga
     3481 acaaattata atcattctga ttggtctggt tgcaagttta atgaaacccc tgttgaccgt
     3541 aagcaagttt ttaaaacttt caaatgtaaa ctaactgatc aacaaaaaga cttgtatgat
     3601 gccacagatc ctttcattta tgatgataaa tgcgaaggaa ttaaatttgg tcgtgtggtc
     3661 ggtagaaaag cacaggaaga aataaaggcg agcaagaaat tatttaagaa ttcattgtca
     3721 tacgatctgt taagtgagtt tgaaaatgaa atagagccat acctggatca taataataat
     3781 ctgctggaaa ctgataaaca ctttgatctt cgactagctc aacaatttat attcaatcgt
     3841 gttatagagc ttggttggga tccggagaag catggtaatt ttgaccaaca aataggaact
     3901 ggacgtggac gtagagaggc attccaagaa cggattggta aaaaatacca atggattgct
     3961 tattatgaat acatggcaag gctagccgat aattttactc gttttgaagg ttatggtgac
     4021 gaacgaaagg aaaatccata ccaagggcca tgggagcctt acgtaagaga tatagatccc
     4081 actatcttac ttaaagaaac tggaacgaaa aaaataagca ataaagaaat gtggtggctt
     4141 aatgatgaag tgtttgattg gacttgctct aatgaagact gggttaaaag ttctactact
     4201 ataactaatt catatgcttt tattgaagtt aaagatgata atggtgatga atggatagta
     4261 ttagaaagtc atccatcatg gaaagaacca aaaattattg gaaacgatga ttgggggcac
     4321 ccacgaaaag aggtttggta tcagatcaga agttatatcg ttaaagttga agaatttgaa
     4381 aattttagat gttgggcaat agctcaagac tttatgggca ggtggatgcc ggaatgtact
     4441 gatagatacc aattatttaa tagggagtac tattggtccg aagcatttaa gtcttttaaa
     4501 tcagattatt atggtggatc tgactggact tcggtaacag accgggagtc tggagctaag
     4561 atagctgatg ttagtgtcac ttcgattaat tatttgtggg aagaggagtt cgacaaatca
     4621 aaaatagaaa ctttgaattt tttgaagcct agtaacttaa tctttgaaaa gatgggatta
     4681 aaaagtgggg aagtagaggg tagcttcaat gatgaaaatg gaactatggt ttgctttgca
     4741 gctgaagctg tatatgcttc aaagccgcat ctacttgtta aaaaagaacc atttttaaca
     4801 atgttaaggg acaatggttt tgaaatcgtt tggacattat taggtgaaaa gggcgttata
     4861 gggggctcac tcatatcaag tcatcattat ggtcgacagg agtttagtgg agcattttat
     4921 tatgaagaca gtcagctaac aggaagtcat aaaactagct ttacgagata aaaatgaatc
     4981 tcagagctga atatataagt agtattagaa accgggttat acttaagaaa tcaatcttaa
     5041 gtgtggcagt cgaatggtag ctaatatgct agcggcgcta atgcctgttt gttgctcata
     5101 acaggcattc actttagtta tggcagaaaa gtatacatgc tgggttggga aagtgtgaaa
     5161 gaaaggaaga ttgctgcgcc gtttgtcgtc acgtttatct tcattggcta tgcacgcaat
     5221 aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaacctc
     5281 aggcatttga gaagcacacg gtcacactgc ttccggtagt caataaaccg gtaaaccagc
     5341 aatagacata agcggctatt taacgaccct gccctgaacc gacgaccggg tcgaatttgc
     5401 tttcgaattt ctgccattca tccgcttatt atcacttatt caggcgtagc accaggcgtt
     5461 taagggcacc aataactgcc ttaaaaaaat tacgccccgc cctgccactc atcgcaatac
     5521 tgttgtaatt catttaacat tctgccgaca tggaagccat cacagacggc atgatgaacc
     5581 tgaatcgcca gcggcatcag caccttgtcg ccttgcgtat aatatttgcc catagtgaaa
     5641 acgggggcga agaagttgtc catattggcc acgtttaaat caaaactggt gaaactcacc
     5701 cagggattgg ctgagacgaa aaacatattc tcaataaacc ctttagggaa ataggccagg
     5761 ttttcaccgt aacacgccac atcttgcgaa tatatgtgta gaaactgccg gaaatcgtcg
     5821 tggtattcac tccagagcga tgaaaacgtt tcagtttgct catggaaaac ggtgtaacaa
     5881 gggtgaacac tatcccatat caccagctca ccgtctttca tcgccatacg gaactctgga
     5941 tgagcattca tcaggcgggc aagaatgtga ataaaggccg gataaaactt gtgcttattt
     6001 ttctttacgg tctttaaaaa ggccgtaata tccagctgaa cggtctggtt ataggtacat
     6061 tgagcaactg actgaaatgc ctcaaaatgt tctttacgat gccattggga tatatcaacg
     6121 gtggtatatc cagtgatttt tttctccatt ttagcttcct tagctcctga aaatctcgat
     6181 aactcaaaaa atacgcccgg tagtgatctt atttcattat ggtgaaagtt ggaacctctt
     6241 acgtgccgat caacgtctca ttttcgccaa aagttggccc agggcttccc ggtatcaaca
     6301 gggacaccag gatttattta ttctgcgaag tgatcttccg tcacaggtat ttattcggcg
     6361 caaagtgcgt cgggtgatgc tgccaactta ctgatttagt gtatgatggt gtttttgagg
     6421 tgctccagtg gcttctgttt ctatcagctg tccctcctgt tcagctactg acggggtggt
     6481 gcgtaacggc aaaagcaccg ccggacatca gcgctagcgg agtgtatact ggcttactat
     6541 gttggcactg atgagggtgt cagtgaagtg cttcatgtgg caggagaaaa aaggctgcac
     6601 cggtgcgtca gcagaatatg tgatacagga tatattccgc ttcctcgctc actgactcgc
     6661 tacgctcggt cgttcgactg cggcgagcgg aaatggctta cgaacggggc ggagatttcc
     6721 tggaagatgc caggaagata cttaacaggg aagtgagagg gccgcggcaa agccgttttt
     6781 ccataggctc cgcccccctg acaagcatca cgaaatctga cgctcaaatc agtggtggcg
     6841 aaacccgaca ggactataaa gataccaggc gtttccccct ggcggctccc tcgtgcgctc
     6901 tcctgttcct gcctttcggt ttaccggtgt cattccgctg ttatggccgc gtttgtctca
     6961 ttccacgcct gacactcagt tccgggtagg cagttcgctc caagctggac tgtatgcacg
     7021 aaccccccgt tcagtccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc
     7081 cggaaagaca tgcaaaagca ccactggcag cagccactgg taattgattt agaggagtta
     7141 gtcttgaagt catgcgccgg ttaaggctaa actgaaagga caagttttgg tgactgcgct
     7201 cctccaagcc agttacctcg gttcaaagag ttggtagctc agagaacctt cgaaaaaccg
     7261 ccctgcaagg cggttttttc gttttcagag caagagatta cgcgcagacc aaaacgatct
     7321 caagaagatc atcttattaa tcagataaaa tatttctaga tttcagtgca atttatctct
     7381 tcaaatgtag cacctgaagt cagccccata cgatataagt tgtaattctc atgttagtca
     7441 tgc
//
