LOCUS       sequence-304769-        9509 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses the AVAST type 3 defense system.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9509)
  AUTHORS   Gao L, Altae-Tran H, Bohning F, Makarova KS, Segel M, Schmid-Burgk 
            JL, Koob J, Wolf YI, Koonin EV, Zhang F
  TITLE     Diverse enzymatic activities mediate antiviral immunity in 
            prokaryotes.
  JOURNAL   Science. 2020 Aug 28;369(6507):1077-1084. doi: 
            10.1126/science.aba0372.
  PUBMED    32855333
REFERENCE   2  (bases 1 to 9509)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1126/science"; 
            journalName: "Science"; date: "2020-08-28- 28"; volume: "369"; 
            issue: "6507"; pages: "1077-1084"
COMMENT     Sequence Label: pLG019 (AVAST type 3)
FEATURES             Location/Qualifiers
     source          1..9509
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      7289..7336
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     CDS             complement(7556..8215)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     8130..8149
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(8216..8318)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     primer_bind     complement(8710..8727)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8844..9389)
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
     primer_bind     complement(8924..8943)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgagatc
       61 ccggtgccta atgagtgagc taacttacat taattgcgtt gcgcaacaat tttttgccat
      121 aagacgcttt cctgaaactc ttctcattct cagcaggaaa gcgttctctt ctcaatactc
      181 tctggttata gagtattaaa aaataaggag ttataatcct tgtagcccaa ctgacataag
      241 gacgatgctc aatgtctgac agcctgcttg ttcgcaccag tagagatggc gatcagtttc
      301 attatctttg ggcggctcgc cgcgcccttc gactactgga acctcagtca actcttgttg
      361 ccctgaccat tgaaggggca tcaacgacgg aaatgggctc tcagccagtg gttgaggatg
      421 gggaggagct gattgatatt gctgaatatt acggcagtaa cgagctcgca acagcaacaa
      481 ctgttcgtta tatgcagcta aagcattcaa caatgcactc agatactcca tttcccccta
      541 gtgggttaca aaaaaccatc gaaggttttg caacccgtta taaggcactt atacaaaaaa
      601 taccggtaga aacgttacgc actaaactcg agttctggtt tgtgacgaac cgtccagtca
      661 gtagcagctt cagtgaagcg atcaatgatg ccgcgaacca acacgttaca cgccatccac
      721 atgatctggc gaaacttgag aaatttaccg ggcttcaagg cgctgagtta tcgatattct
      781 gccagctttt acatatagaa ggtcagcagg acgatttatg gagtcagcgg aatatcctgc
      841 taagagaatc agcgggatat ctccccgacc tggatactga agcccctctg aaattaaaag
      901 agctggttaa cagaaaagcg ttaaccgaaa gcgccgcaaa tccttccatt accagaatgg
      961 atgtgttgcg tgctttgggg gtggatgaaa cagatctttt tcctgcgccc tgtcgtattg
     1021 aaagaataga aaattccgtc tcaagaactc aagaggcgac gctggttcaa cgtgttgttg
     1081 aagcattcgg cgcacctgtg atcatccatg ccgatgccgg tgtggggaaa tcaattttct
     1141 ctactcatat agaggagcat cttcccactg gttctgttag catcttatat gactgtttcg
     1201 gactgggtca gtaccgtaac gcgtcttcct accgccacca ccatcgtaca gcattggttc
     1261 agatggctaa tgaaatggca tctcgtggtc tctgtcatcc attgatccca aatgctggta
     1321 ctggcatatc ccagtatatg cgtgcgtttc tgcatcgcct ttctcagagc atttcaatac
     1381 tccgggcctc tgagcccttg gccgtattgt gtattattat tgatgctgcg gacaatgcac
     1441 agatggcggc ggaagaaatc ggtgaaacgc gttcttttat caaagattta attagagaaa
     1501 agcttcctga tggagtctgc cttgttgcac tttgccgacc ttatagacgg gaattacttg
     1561 atccacctcc tgaagcactc acattatccc tacaaacttt taatcgcgat gagacagccg
     1621 ctcatcttca ccaaaaattt ccagatgcca gcgaaagtga tgttgacgag ttccatcgtc
     1681 taagctcttg caacccccgg gttcaggctc tgtcattatc acaaaatctt ccactgaacg
     1741 acacattgag acttttgggg ccaaatccca aaacggtaga agatactatt ggtgaagtgc
     1801 tggaaaaatc cattgctcgc ttacgtgata cagccggaat atctgaacgt gctcaaattg
     1861 atacgatttg ttccgcactg gcaatattgc gtccattaat tccattatct gtgctatctg
     1921 ccatttccgg agtagctggt tctgctatta aaagtttcgc acttgatctg ggacgcccgt
     1981 taatcgttag tggcgagact attcagttct ttgatgaacc ggccgaaaca tggtttcaga
     2041 ggcgctttag gccatcggcc gctgatctgc atcagtttat tactaaactg agaccactaa
     2101 caaaagatag ttcctatgca gcatcagttt tacctgcatt gatgctggaa ggaaaccagc
     2161 tttctgaact gatcgagcta gcgatatcct cacaagctct gcctgaaacc agcgcggttg
     2221 aacgcaggga catagaactt caaagattac agtttgcgtt aaaagcagcc ttacgcacag
     2281 gtcgatacca ggatgcggct aaactggcac tgaaagctgg tggagaatgc gcgggtgaca
     2341 acaggcaaag agtcctgctg agggacaata tcgatctggc agcaaaattt gtgggaagca
     2401 acggcgttca ggaactggtt tcccgtaacg catttccaga tactggctgg cctggctcca
     2461 gaaatgctta ttatgccgca atactttccg aatatcctga actctcagga gaggcccgca
     2521 gtcgccttcg actcaccatg gagtggttaa caaactggag tcaattacca gatgatgagc
     2581 ggagcaggca aaatgttacc gatcaggaca gagcggtaat gctcattgcc tgcctgaata
     2641 ttcatggcgc ggaagcggca gcaagggagc tcagaaggtg gcggcctcga aaactatctt
     2701 ttgacgctgg aaaaattgtt gccatgcagt tactggccca cgcccgttat gatgaacttg
     2761 atcagttggc tattgcggct ggaaacgata tcagcctggt tatgggaatt gtactggaag
     2821 caagaaaact tcaccgtcca gtcgctgaac aagcaatcag aagaacctgg cgcttgttaa
     2881 aaagtcagcg agtcagcatt aaagacagaa accacgctaa taaccagaca atagcagcaa
     2941 tcactggcat ggttgaaatg gcgcttatcc aatctgtttg tactgaatca gaaagcatcc
     3001 agttgttgga tcgttattta ccaaaggttc ccccctatgc tctgacttct gagtatagta
     3061 aagaaagagt tgcttacgtc cgggcatatg ctctgcaggc aaacctgatg ggctctcaat
     3121 tagcgcttag cgatttagcc tccacagagg ttaaaaaaga acttatggct gaaaaacgcc
     3181 acggcgaatc tgatgacctg cgtcaactga agcagtacag cggagtatta atcccttggt
     3241 ataatttatg ggccaaagta attcttggta aaacaaggaa agcagactta gaaagtgagc
     3301 taagtgatac tcaaaaagaa tcgacggcta ttaaaggtca ttcttactct gagcattcat
     3361 tatcatcaaa tgagatcgca aatgtatggt ttgatattct gatcgaagca ggtaatgtat
     3421 caaaagacga tgtggaaaac atcatcaaat ggagtcagca taaagggaat agagtattca
     3481 caccaacgct tcaccgtttc agttctgtat gtgcagagat ttcagggctt ggagagcttt
     3541 catatcactt cgcagaactt gccttatctt tatggaggga tgagcactct gatgctcaga
     3601 tcaaagctga cggctatata gacctttccc gttcactcat ttcacttgat gaaccagaag
     3661 ctaaagaata ctttaaccaa gcgattgaag ttacaaataa gttaggcgat gaaaatttaa
     3721 gtcgatggga agcgatactt gatcttgctg aatatgttgc tggtaaaacg caagtccctc
     3781 ctgaaatttc ctataaacta gcccgatgtg cggaactaac cagagaatat gttgatcgtg
     3841 ataaacattt tgcatggagt gatactgttg agattttggc tgagttatgt ccatcttcag
     3901 ccctagcaat aataagtcgt tggcgtgacc gtacatttgg caatcataga agcatactgg
     3961 catggaccat tgagcatctt gtaaagaaaa ataaaattaa tgcactcgat gcacttcctt
     4021 taatcacatt tgagaatgat tggcataaat gcgacttgct tgattcagtt ttatcctcgt
     4081 gtactgatga caaagataag atcatggcat tcgaagtggt ttaccactat acaaaattta
     4141 acgtacaaaa tatccaaaat cttaaaaagc tggatgctat ttctacatca ttaggtattg
     4201 aacacacaga actgaaagaa agaatttcag gtctacaaca tactgagacg gtttcaaaaa
     4261 aatccagtct ctcatcgaat gataatgagc aaggccatga ccaggaatgg gagtccattt
     4321 ttaaagattg tgatttatcg tctattgatg gtattagtgc agcatacgaa aaatttcgta
     4381 atgttcctga attctattcc aaagaaacct tcatcaagaa agcaataagc cgagttaaga
     4441 cgggcaaaga atgtagtttc attactgcca ttggtgctat atttcactgg gggctttatg
     4501 attttaaata tattcttgaa tctatacccg acgaatggac atctcgttta agcattaaaa
     4561 ccaccctggc aggtttaata aaagaatatt gccaacgctt ctgtatgcga atcagaaaaa
     4621 gtcgcgttta cgagattttt cccttcagtc tggccagcag gctttctggt ataagtgaaa
     4681 aagagatttt cggtattacc ctggaggcca ttgcagaatc gccagagccc gcaaactctg
     4741 accgtttatt tagccttcct ggccttcttg ttagtaaact ggagagtaat gaagcgttag
     4801 atgtattatc ttatgccttg gatttattcg acgaggtgct aaaagatgag gatggtgacg
     4861 gcccatggaa cgagaaatta tctccgccaa ctcatgtaga ggattcactt gcaggctata
     4921 tttgggcgcg gctgggttct ccggaggcgg aaatgcgctg gcaggcagca catgcggttc
     4981 tggcactatg tcgaatgagt cgtacatgcg ttatacaagg aattttccag cacgcaataa
     5041 atgctaccac tttacctttt tgtgatcgca atctgccctt ttataccctc catgctcaat
     5101 tgtggttgat gatcgctgct gcaagggttg cgctggatga tggaaaatcg ctgattccca
     5161 atattggtta tttctaccat tatgccacta ctgatcagcc acatgtatta atccgtcatt
     5221 ttgctgccag aactttactt gcactgcatg atagcgacct gatctctatc ccagcacaag
     5281 aagagaataa actccgaaat ataaaccagt ctacgactct ccctgtgctt gataaggttg
     5341 aagatcatag aggtgaagat tcatatactt ttggtatcga ctttggccct tactggctaa
     5401 aacctctggg acgttgtttc ggtgtatctc aaaaacagtt agaacctgaa atgcttcgca
     5461 ttattcgtga tgttcttggt tttaaaggta gccgcaactg ggatgaggat gagcgtaata
     5521 aacgacgcta ttatcaagac agagataatc atcacagtca tggttcctat ccacgggtcg
     5581 atgactacca tttttacttg tcataccatg caatgtttat gaccgctggg cagttattag
     5641 cgacaaaacc attagttggt agtgactacg acgatgtcga ggatgttttc caggactggt
     5701 taagaagaca tgatatttct cggaacgatc atcgctggct cgccgatcgg agagatattc
     5761 cccccaaaga gcgctccagt tggcttaata gcagttctga caatagggat gaatggctag
     5821 cgtcaatctc tgaaaatgta tttaacgaaa cactatgtcc cagccccgga ctattaacgc
     5881 tatggggacg ttggtctgac gtttgttcag atcgaaaaga atctattatt gtccattctg
     5941 cgttagtatc gccggagcga tctttatcgc tcctcagagc attacaaaca actaaaaatg
     6001 tatatgacta taaaatccct gatgctggag ataatcttga aatagatcac gcacactatc
     6061 agctaaaagg atggattaaa gatattgctg aatactgtgg aattgatgag tttgatccct
     6121 gggcaggtaa tgtaaggttt ccaatcccag aaccagcctc atttatcatt gatgcgatga
     6181 aattaactac tgataaagat catcgggtat ggtattcacc ttctgatgtt gaaccggcga
     6241 tgatttccag tatctggggc catctatcag gtaaaaatga tgaggaaaaa tcacatggtt
     6301 ataggctatg tgcttcaata cacttcataa aatcagcatt agaaacattc aacatggatc
     6361 tcattttaga ggttgatgtt gatcgctatt cacggaacag cagatatgaa cggaataatg
     6421 aaaatgagct cgacaatatc ccttcaagca ctcgactctt cctcttccga catgacggaa
     6481 ccatccacac gctatacggc aattatagaa atggggaaaa aactagttga tgagcttgag
     6541 ctaaatgact ctgttgatac attaagcaga tggatggctc atcatatcgc agagctcatt
     6601 tatgatgctg aacattgtac agacgacatc gtccgtacag ctaaacaagc ggagattagg
     6661 gactctatct ggtcattctg gtctaacaga tacgaattgc caattggtag cagaccattt
     6721 caggagctcg aacctattct aagaacctta aaaggtcttg atcctgaaaa tgagcaaccg
     6781 agattttttt caccttaccg agatctaatt aatgtagaaa aagaaaccag tgaggtccaa
     6841 aaatggctaa ccgccgctaa ggatattgat tcagcagcaa aaatactgat tgattactgt
     6901 ttatcgttag cagcagaaaa tgctatcgat aaatcccaag aatgggtgga attagcacag
     6961 aaagctggat tgaacaaaga tgttgatctg cttgaaattc gtatctttca gttacgaggt
     7021 accccagcca atacagacaa tcccaataat gcacaacgga gaatactgga aaaaaggcaa
     7081 aaaaggcttg aagcttttct cttattgggc tcccagttaa acgaacaact caaatctcag
     7141 cttgaagcct taccagcaat tgaggatgag ccaacggatg acgacgaaga cttttgatat
     7201 gacttgcttt agcactggag acggctcaca agacggacca cataatagcc taacccaaga
     7261 cttttctact agtcctaatg cgcaataact agcataaccc cttggggcct ctaaacgggt
     7321 cttgaggggt tttttgctga aacctcaggc atttgagaag cacacggtca cactgcttcc
     7381 ggtagtcaat aaaccggtaa accagcaata gacataagcg gctatttaac gaccctgccc
     7441 tgaaccgacg accgggtcga atttgctttc gaatttctgc cattcatccg cttattatca
     7501 cttattcagg cgtagcacca ggcgtttaag ggcaccaata actgccttaa aaaaattacg
     7561 ccccgccctg ccactcatcg caatactgtt gtaattcatt taacattctg ccgacatgga
     7621 agccatcaca gacggcatga tgaacctgaa tcgccagcgg catcagcacc ttgtcgcctt
     7681 gcgtataata tttgcccata gtgaaaacgg gggcgaagaa gttgtccata ttggccacgt
     7741 ttaaatcaaa actggtgaaa ctcacccagg gattggctga gacgaaaaac atattctcaa
     7801 taaacccttt agggaaatag gccaggtttt caccgtaaca cgccacatct tgcgaatata
     7861 tgtgtagaaa ctgccggaaa tcgtcgtggt attcactcca gagcgatgaa aacgtttcag
     7921 tttgctcatg gaaaacggtg taacaagggt gaacactatc ccatatcacc agctcaccgt
     7981 ctttcatcgc catacggaac tctggatgag cattcatcag gcgggcaaga atgtgaataa
     8041 aggccggata aaacttgtgc ttatttttct ttacggtctt taaaaaggcc gtaatatcca
     8101 gctgaacggt ctggttatag gtacattgag caactgactg aaatgcctca aaatgttctt
     8161 tacgatgcca ttgggatata tcaacggtgg tatatccagt gatttttttc tccattttag
     8221 cttccttagc tcctgaaaat ctcgataact caaaaaatac gcccggtagt gatcttattt
     8281 cattatggtg aaagttggaa cctcttacgt gccgatcaac gtctcatttt cgccaaaagt
     8341 tggcccaggg cttcccggta tcaacaggga caccaggatt tatttattct gcgaagtgat
     8401 cttccgtcac aggtatttat tcggcgcaaa gtgcgtcggg tgatgctgcc aacttactga
     8461 tttagtgtat gatggtgttt ttgaggtgct ccagtggctt ctgtttctat cagctgtccc
     8521 tcctgttcag ctactgacgg ggtggtgcgt aacggcaaaa gcaccgccgg acatcagcgc
     8581 tagcggagtg tatactggct tactatgttg gcactgatga gggtgtcagt gaagtgcttc
     8641 atgtggcagg agaaaaaagg ctgcaccggt gcgtcagcag aatatgtgat acaggatata
     8701 ttccgcttcc tcgctcactg actcgctacg ctcggtcgtt cgactgcggc gagcggaaat
     8761 ggcttacgaa cggggcggag atttcctgga agatgccagg aagatactta acagggaagt
     8821 gagagggccg cggcaaagcc gtttttccat aggctccgcc cccctgacaa gcatcacgaa
     8881 atctgacgct caaatcagtg gtggcgaaac ccgacaggac tataaagata ccaggcgttt
     8941 ccccctggcg gctccctcgt gcgctctcct gttcctgcct ttcggtttac cggtgtcatt
     9001 ccgctgttat ggccgcgttt gtctcattcc acgcctgaca ctcagttccg ggtaggcagt
     9061 tcgctccaag ctggactgta tgcacgaacc ccccgttcag tccgaccgct gcgccttatc
     9121 cggtaactat cgtcttgagt ccaacccgga aagacatgca aaagcaccac tggcagcagc
     9181 cactggtaat tgatttagag gagttagtct tgaagtcatg cgccggttaa ggctaaactg
     9241 aaaggacaag ttttggtgac tgcgctcctc caagccagtt acctcggttc aaagagttgg
     9301 tagctcagag aaccttcgaa aaaccgccct gcaaggcggt tttttcgttt tcagagcaag
     9361 agattacgcg cagaccaaaa cgatctcaag aagatcatct tattaatcag ataaaatatt
     9421 tctagatttc agtgcaattt atctcttcaa atgtagcacc tgaagtcagc cccatacgat
     9481 ataagttgta attctcatgt tagtcatgc
//
